Standard name
Human Ortholog
Description Sorting nexin family member; required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0.09 0 0 0.05 0 0.06 0 0 0.09 0.05 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 0.06 0.1 0.1 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.21 0.27 0.35 0.17 0.18 0.21 0.24 0.24 0.23 0.22 0.21 0.18 0.22 0.36 0.48 0.17 0.2 0.18 0.09 0.06 0.08 0.09 0.08 0.12
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.58 0.6 0.43 0.34 0.4 0.4 0.34 0.25 0.23 0.23 0.19 0.25 0.57 0.51 0.44 0.45 0.42 0.53 0.7 0.7 0.54 0.62 0.59 0.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Mitochondria 0.13 0 0 0.07 0 0.17 0.12 0.15 0.44 0.32 0.5 0.36 0 0 0 0.08 0 0 0.06 0 0.15 0.08 0.08 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.08 0 0 0 0.07 0.05 0.07 0.08 0.06 0 0 0 0.05 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
SpindlePole 0.17 0.13 0.17 0.46 0.43 0.25 0.36 0.35 0.14 0.27 0.12 0.29 0.22 0.22 0.18 0.29 0.32 0.3 0 0 0 0.08 0.05 0.09
Vac/Vac Membrane 0.07 0.1 0.1 0.05 0.07 0 0 0 0.05 0 0 0 0.1 0.05 0.05 0.17 0.17 0.11 0 0.06 0.09 0.08 0.13 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 8 5 6 5 1 8 15 3 7 7 5 6 5 0 20 9 18 6 5 3 0 1 2
Bud 7 2 2 1 2 7 9 11 13 19 8 10 2 0 1 2 0 3 1 1 1 0 0 1
Bud Neck 0 0 0 2 0 0 0 1 1 0 2 0 1 0 0 0 0 0 2 1 2 1 2 1
Bud Site 0 0 0 0 0 0 3 0 0 0 1 3 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 1 2 0 1 0 0 1 2 2 1 0 0 0 0 0 0
Cytoplasm 58 50 39 25 30 26 48 43 30 40 31 30 23 49 79 36 36 46 27 13 12 11 13 19
Endoplasmic Reticulum 6 9 2 1 2 5 4 3 2 1 1 0 3 3 1 6 8 5 5 2 2 0 2 2
Endosome 157 109 48 49 66 51 68 44 30 42 28 42 60 69 71 95 75 135 223 157 86 78 100 95
Golgi 0 5 0 1 2 0 1 0 0 0 0 0 0 0 1 13 10 7 2 6 1 0 1 0
Mitochondria 35 2 1 10 3 21 25 27 58 59 73 61 0 0 0 17 8 11 20 5 24 10 13 9
Nucleus 0 1 1 0 1 1 2 4 1 0 4 2 1 0 0 2 2 0 0 1 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 2 3 0 12 6 5 9 13 6 13 12 10 0 1 4 11 7 10 0 0 0 0 0 0
Peroxisomes 7 2 1 5 7 5 9 2 3 3 4 5 2 3 9 1 1 6 0 1 0 0 0 0
SpindlePole 45 24 19 67 70 31 72 62 18 49 18 50 23 30 30 61 57 76 13 8 6 10 8 13
Vac/Vac Membrane 19 18 11 8 11 3 9 4 6 8 2 6 11 7 8 35 31 28 12 14 14 9 21 9
Unique Cell Count 272 183 111 146 164 126 201 176 132 182 147 171 105 135 163 212 180 257 319 224 162 127 171 158
Labelled Cell Count 343 233 129 187 205 156 269 229 172 243 191 225 132 167 205 301 246 347 319 224 162 127 171 158


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 7.4 7.1 6.6 7.7 6.6 6.6 7.0 5.6 6.1 5.7 6.1 7.4 7.5 7.6 10.5 11.8 9.8 6.6 6.9 7.3
Std Deviation (1e-4) 0.7 0.9 1.6 1.6 1.8 1.7 1.4 1.5 1.2 1.2 1.2 1.6 1.2 1.1 1.3 2.1 3.4 2.4 1.5 1.2 1.4
Intensity Change (Log2) – – – -0.11 0.11 -0.11 -0.11 -0.03 -0.36 -0.22 -0.32 -0.22 0.06 0.07 0.1 0.56 0.72 0.46 -0.11 -0.04 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 1.3 0 0 0.1 0 0.4 0 0 1.6 0.2 0.9
Bud 0 0 0 0 1.8 2.6 2.8 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.3 -3.2 -2.5 -2.1 -2.0 -2.1 -2.5 -2.5 -3.4 -2.1 0.2 2.2 -3.7 -2.9 -3.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.6 -0.5 -0.4 -1.6 -3.2 -3.4 -3.6 -4.2 -3.3 2.0 1.2 0.1 0.3 -0.3 1.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.2 3.5 4.0 7.8 6.5 8.6 6.9 0 0 0 2.6 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.1 0 0 0 2.9 0 0 3.1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 4.8 4.4 1.4 3.5 3.3 -0.8 1.9 -1.1 2.3 0.9 1.0 0.3 2.3 2.7 2.5
Vacuole -1.3 -1.0 -2.5 -1.9 -2.8 -1.6 -1.9 -3.1 -2.2 0.1 -1.4 -1.6 1.6 1.7 0.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2617 0.9006 0.8532 0.5274 0.3132 0.864 1.2562 1.509 1.395 1.2742 1.0872 1.5482 1.3483 1.6877 1.2594 1.3175 2.0967 1.387
Actin 0.021 0.0034 0.004 0.0001 0.0008 0.0021 0.0032 0.001 0.0016 0.0036 0.0146 0.0038 0.0284 0.0002 0.022 0 0.0683 0.029
Bud 0.0006 0.0029 0.0043 0.0002 0.0001 0.0007 0.0003 0.0009 0.0021 0.0005 0.0006 0.0003 0.0035 0.0006 0.0017 0.0002 0.0026 0.0003
Bud Neck 0.0011 0.001 0.0006 0.0002 0.0004 0.001 0.0007 0.0005 0.0011 0.0019 0.0005 0.0022 0.0021 0.0004 0.0011 0.0003 0.0106 0.001
Bud Periphery 0.0006 0.0013 0.0021 0.0001 0.0001 0.0007 0.0003 0.0009 0.0032 0.0008 0.0007 0.0004 0.0022 0.0003 0.0017 0.0001 0.0035 0.0004
Bud Site 0.0049 0.0242 0.0028 0.0004 0.0004 0.0005 0.0028 0.0053 0.0047 0.0006 0.0009 0.0007 0.0142 0.0025 0.0087 0.0002 0.0408 0.0007
Cell Periphery 0.0003 0.0005 0.0002 0 0 0.0001 0.0002 0.0002 0.0004 0.0001 0.0001 0.0001 0.0011 0.0001 0.0002 0 0.0011 0.0001
Cytoplasm 0.1258 0.2878 0.1846 0.1636 0.1081 0.2274 0.1196 0.1029 0.1626 0.1503 0.051 0.1411 0.1529 0.3051 0.1835 0.1538 0.0524 0.2009
Cytoplasmic Foci 0.258 0.2429 0.2618 0.2235 0.224 0.3097 0.2317 0.3364 0.2671 0.228 0.2254 0.2968 0.2109 0.2619 0.2563 0.1782 0.1998 0.3382
Eisosomes 0.0003 0.0001 0.0002 0 0.0001 0 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0003 0 0.0001 0 0.0004 0.0002
Endoplasmic Reticulum 0.0071 0.0046 0.0034 0.0036 0.002 0.0032 0.0045 0.0025 0.0029 0.0041 0.0022 0.0029 0.0132 0.0043 0.0037 0.0028 0.0041 0.0042
Endosome 0.2982 0.2854 0.3413 0.3186 0.4566 0.3079 0.4303 0.4289 0.3683 0.4372 0.5393 0.4189 0.3632 0.3269 0.3785 0.5635 0.409 0.2981
Golgi 0.0252 0.011 0.0168 0.0074 0.0321 0.0173 0.0361 0.0294 0.0265 0.0282 0.0573 0.0348 0.0265 0.015 0.0367 0.0164 0.0765 0.0256
Lipid Particles 0.0493 0.0137 0.0295 0.0522 0.0367 0.0327 0.0465 0.0243 0.0408 0.0056 0.0133 0.0216 0.0558 0.0176 0.0183 0.0059 0.0422 0.0337
Mitochondria 0.0138 0.0058 0.0044 0.0017 0.0311 0.0026 0.0084 0.0024 0.0208 0.0467 0.0138 0.0151 0.0064 0.0018 0.0052 0.0007 0.0176 0.0112
None 0.0764 0.0801 0.0858 0.1792 0.0776 0.0586 0.0653 0.0238 0.0532 0.0201 0.0127 0.0212 0.0412 0.0248 0.0355 0.0318 0.0088 0.025
Nuclear Periphery 0.021 0.0014 0.0027 0.0026 0.0012 0.0007 0.0092 0.0008 0.0008 0.0025 0.001 0.0007 0.0107 0.001 0.0011 0.0013 0.0088 0.0026
Nucleolus 0.0019 0.0021 0.0038 0.0005 0.0002 0.0002 0.0005 0.0003 0.0005 0.0002 0.0002 0.0001 0.0077 0.0002 0.0002 0.0001 0.0075 0.0004
Nucleus 0.0081 0.0024 0.0084 0.0013 0.0006 0.0008 0.0024 0.0005 0.0008 0.0015 0.0005 0.0004 0.0096 0.0008 0.0005 0.0007 0.0029 0.0006
Peroxisomes 0.0307 0.0062 0.0176 0.0156 0.0143 0.0204 0.0192 0.0244 0.0237 0.0418 0.0523 0.0279 0.0196 0.0126 0.0292 0.0016 0.0208 0.0153
Punctate Nuclear 0.0181 0.0025 0.0076 0.0028 0.0028 0.0046 0.0024 0.0016 0.0014 0.0008 0.0007 0.0012 0.0083 0.0023 0.0019 0.0007 0.0061 0.0056
Vacuole 0.0272 0.0194 0.0157 0.0223 0.0077 0.0071 0.0138 0.011 0.0146 0.0201 0.0095 0.0077 0.0189 0.0191 0.0112 0.036 0.0133 0.0052
Vacuole Periphery 0.0104 0.0014 0.0027 0.0039 0.0031 0.0015 0.0023 0.0019 0.0028 0.0053 0.0033 0.002 0.0033 0.0024 0.0028 0.0057 0.003 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.3283 11.2846 11.038 13.1969 11.9032 10.3226 11.7988 13.6516 14.7088 12.5684
Translational Efficiency 0.8102 0.871 0.8504 0.8352 0.7337 1.0108 0.9151 0.8435 0.8463 0.7834

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Sorting nexin family member; required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate
Localization
Cell Percentages endosome (39%), cytoplasm (14%), mixed (26%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Atg20

Atg20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available