Standard name | |
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Human Ortholog | |
Description | Adenosine 5'-monophosphoramidase; interacts physically and genetically with Kin28p, a CDK and TFIIK subunit, and genetically with CAK1; member of histidine triad (HIT) superfamily of nucleotide-binding proteins; protein abundance increases in response to DNA replication stress; human homolog HINT1 can complement yeast hnt1 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 3 | 6 | 8 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 1 | 5 | 6 | 5 | 10 | – | 12 | 15 | 13 | 17 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Cytoplasm | 223 | 229 | 177 | 242 | 314 | 285 | 415 | – | 336 | 307 | 310 | 338 | 379 | 361 | 477 | 86 | 89 | 69 | 214 | 208 | 173 | 199 | 383 | 343 |
Endoplasmic Reticulum | 0 | 1 | 0 | 4 | 6 | 8 | 23 | – | 17 | 18 | 14 | 33 | 2 | 3 | 0 | 3 | 2 | 2 | 0 | 0 | 0 | 4 | 6 | 4 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 3 | 3 | 2 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 0 | 1 | 0 | 0 | 0 | 4 | 0 | – | 3 | 2 | 5 | 13 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 1 | 0 | 3 | 2 | 10 | – | 11 | 12 | 9 | 12 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 6 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 1 | 29 | 19 | 17 | 55 | – | 26 | 34 | 20 | 83 | 0 | 0 | 3 | 0 | 0 | 1 | 0 | 3 | 0 | 3 | 2 | 1 |
Unique Cell Count | 224 | 231 | 178 | 266 | 335 | 313 | 503 | 395 | 382 | 364 | 485 | 381 | 363 | 479 | 87 | 92 | 70 | 221 | 223 | 179 | 217 | 401 | 362 | |
Labelled Cell Count | 224 | 234 | 180 | 280 | 348 | 326 | 517 | 413 | 400 | 381 | 520 | 384 | 365 | 481 | 92 | 95 | 72 | 221 | 223 | 179 | 217 | 401 | 362 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 31.0 | 26.3 | 24.0 | 24.4 | 25.4 | 17.6 | 22.3 | – | 20.2 | 20.4 | 19.0 | 19.7 | 31.8 | 31.0 | 31.5 | 35.7 | 35.2 | 37.7 | 25.9 | 28.8 | 29.6 |
Std Deviation (1e-4) | 4.8 | 5.4 | 4.4 | 4.1 | 4.0 | 4.4 | 4.5 | – | 4.4 | 4.1 | 4.4 | 4.1 | 5.7 | 6.1 | 6.2 | 14.0 | 14.4 | 11.8 | 6.0 | 5.4 | 5.8 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.08 | -0.45 | -0.11 | – | -0.25 | -0.24 | -0.34 | -0.29 | 0.4 | 0.37 | 0.39 | 0.57 | 0.55 | 0.65 | 0.11 | 0.26 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.8 | -3.0 | -3.8 | -5.8 | – | -5.2 | -6.1 | -5.2 | -8.2 | 0.1 | 0 | 0.2 | -0.5 | -1.7 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.9 | – | 2.8 | 2.9 | 0 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 4.3 | 2.8 | 2.8 | 4.3 | – | 3.1 | 3.8 | 2.8 | 5.7 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 18.7087 | 25.4219 | 23.1099 | 21.906 | 21.9905 | 22.3308 | 23.3881 | 29.9791 | 28.3556 | 27.0594 | 25.2369 | 28.8156 | 28.5112 | 33.1717 | 30.9294 | 28.7092 | 25.3473 | 29.6843 |
Actin | 0.0213 | 0.0002 | 0.015 | 0.0001 | 0.0001 | 0.0016 | 0.0386 | 0.0037 | 0.0085 | 0.0027 | 0.0192 | 0.0035 | 0.0113 | 0.0001 | 0.0008 | 0.0002 | 0.0004 | 0.0005 |
Bud | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0009 | 0.0002 | 0.0002 | 0.0031 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0003 | 0 |
Bud Neck | 0.0004 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0014 | 0.0037 | 0.0002 | 0.0004 | 0.0003 | 0.0008 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0.0005 | 0.0006 | 0.0005 |
Bud Periphery | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0024 | 0.0007 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0006 | 0 | 0.0003 | 0 | 0 | 0 | 0.0023 | 0.0002 | 0.0007 | 0.0014 | 0.0021 | 0.0002 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 |
Cytoplasm | 0.832 | 0.9915 | 0.9524 | 0.9827 | 0.9859 | 0.9451 | 0.8122 | 0.9752 | 0.942 | 0.906 | 0.8601 | 0.9522 | 0.9184 | 0.9934 | 0.9641 | 0.957 | 0.9175 | 0.9538 |
Cytoplasmic Foci | 0.0148 | 0.0002 | 0.0029 | 0.0002 | 0.0004 | 0.0009 | 0.0149 | 0.0013 | 0.0034 | 0.0047 | 0.0052 | 0.0025 | 0.0044 | 0.0001 | 0.0014 | 0.0004 | 0.0232 | 0.003 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0036 | 0.0002 | 0.0006 | 0.0004 | 0.0007 | 0.0025 | 0.003 | 0.0005 | 0.0007 | 0.0013 | 0.0013 | 0.0013 | 0.0018 | 0.0001 | 0.0003 | 0.003 | 0.0009 | 0.0006 |
Endosome | 0.0215 | 0 | 0.0007 | 0.0001 | 0.0002 | 0.0009 | 0.0137 | 0.0009 | 0.0014 | 0.008 | 0.0103 | 0.0009 | 0.0037 | 0 | 0.0026 | 0.0012 | 0.0166 | 0.0023 |
Golgi | 0.0096 | 0 | 0.001 | 0 | 0 | 0.0001 | 0.0062 | 0.0015 | 0.0013 | 0.0017 | 0.01 | 0.0003 | 0.0011 | 0 | 0.0007 | 0 | 0.002 | 0.0012 |
Lipid Particles | 0.0188 | 0 | 0.0007 | 0 | 0 | 0 | 0.0102 | 0.0036 | 0.0044 | 0.0001 | 0.0183 | 0.0009 | 0.0059 | 0 | 0.0023 | 0.0001 | 0.0146 | 0.0006 |
Mitochondria | 0.0073 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0065 | 0.0011 | 0.0027 | 0.0361 | 0.0276 | 0.0004 | 0.002 | 0 | 0.0043 | 0.0002 | 0.0005 | 0.0015 |
None | 0.0044 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0004 | 0.0028 | 0.0005 | 0.0007 | 0.0053 | 0.0023 | 0.0016 | 0.0021 | 0.0001 | 0.0008 | 0.0004 | 0.0033 | 0.0003 |
Nuclear Periphery | 0.0064 | 0.0007 | 0.0022 | 0.0022 | 0.0018 | 0.0055 | 0.0095 | 0.0011 | 0.0022 | 0.0032 | 0.0084 | 0.0033 | 0.0047 | 0.0004 | 0.0049 | 0.0049 | 0.0014 | 0.0057 |
Nucleolus | 0.0008 | 0 | 0 | 0 | 0 | 0.0001 | 0.004 | 0 | 0.0002 | 0.0003 | 0.0008 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0345 | 0.0066 | 0.0193 | 0.0137 | 0.0095 | 0.0395 | 0.0478 | 0.0087 | 0.0244 | 0.0163 | 0.0136 | 0.0275 | 0.0376 | 0.0055 | 0.012 | 0.0293 | 0.0097 | 0.0288 |
Peroxisomes | 0.0061 | 0 | 0.0032 | 0 | 0 | 0 | 0.0044 | 0.0006 | 0.0046 | 0.0007 | 0.0128 | 0.0002 | 0.0028 | 0 | 0.0027 | 0 | 0.0039 | 0.0002 |
Punctate Nuclear | 0.013 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0009 | 0.0149 | 0.0002 | 0.0007 | 0.0004 | 0.0033 | 0.0039 | 0.0013 | 0 | 0.0004 | 0.0005 | 0.0013 | 0.0004 |
Vacuole | 0.0028 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.002 | 0.0002 | 0.0008 | 0.0031 | 0.0009 | 0.0002 | 0.0008 | 0 | 0.0007 | 0.0017 | 0.0028 | 0.0003 |
Vacuole Periphery | 0.001 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.001 | 0.0001 | 0.0002 | 0.0028 | 0.001 | 0.0001 | 0.0003 | 0 | 0.0009 | 0.0003 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 191.997 | 190.2445 | 190.2721 | 202.7305 | 196.4467 | 150.2759 | 197.583 | 193.3298 | 181.5762 | 188.7776 |
Translational Efficiency | 1.9818 | 1.9448 | 1.8803 | 1.6667 | 1.7001 | 2.3761 | 1.8547 | 1.7441 | 1.9108 | 1.7126 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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