ORF | |
---|---|
Human Ortholog | |
Description | Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 8 | 5 | 1 | 1 | 4 |
Bud | 0 | 0 | 1 | 0 | 1 | 3 | 6 | 2 | 25 | 11 | 14 | 22 | 0 | 0 | 0 | – | 0 | 0 | 5 | 6 | 5 | 8 | 11 | 18 |
Bud Neck | 1 | 2 | 9 | 2 | 5 | 5 | 7 | 17 | 5 | 12 | 10 | 7 | 0 | 2 | 2 | – | 0 | 0 | 5 | 6 | 1 | 2 | 2 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 15 | 4 | 10 | 13 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Cytoplasm | 111 | 340 | 290 | 285 | 260 | 338 | 373 | 236 | 161 | 155 | 89 | 128 | 185 | 302 | 391 | – | 123 | 132 | 98 | 261 | 224 | 78 | 88 | 124 |
Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 25 | 36 | 0 | 0 | 3 | 0 | 2 | 3 |
Endosome | 8 | 3 | 6 | 4 | 0 | 10 | 13 | 21 | 3 | 10 | 10 | 5 | 1 | 3 | 3 | – | 46 | 39 | 11 | 11 | 6 | 6 | 10 | 9 |
Golgi | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3 | 1 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 26 | 64 | 2 | 8 | 35 | 70 | 172 | 76 | 498 | 379 | 399 | 407 | 1 | 1 | 0 | – | 27 | 11 | 14 | 36 | 47 | 14 | 21 | 20 |
Nucleus | 76 | 39 | 11 | 4 | 5 | 13 | 40 | 76 | 127 | 191 | 198 | 218 | 28 | 14 | 32 | – | 128 | 140 | 111 | 72 | 15 | 73 | 47 | 36 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | – | 0 | 1 | 1 | 2 | 2 | 1 | 2 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 8 | 6 | 2 | 10 | 0 | 0 | 0 | – | 2 | 1 | 3 | 3 | 2 | 10 | 10 | 10 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 6 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 4 | 4 | 2 | 5 | 24 | 26 | 13 | 32 | 25 | 28 | 0 | 1 | 1 | – | 6 | 21 | 28 | 42 | 24 | 35 | 41 | 38 |
Vac/Vac Membrane | 63 | 32 | 12 | 9 | 6 | 31 | 33 | 32 | 24 | 24 | 19 | 24 | 12 | 23 | 33 | – | 79 | 66 | 66 | 86 | 38 | 51 | 70 | 68 |
Unique Cell Count | 260 | 456 | 328 | 305 | 288 | 445 | 600 | 449 | 667 | 682 | 608 | 684 | 221 | 336 | 448 | 359 | 378 | 362 | 571 | 401 | 299 | 332 | 366 | |
Labelled Cell Count | 288 | 482 | 338 | 318 | 316 | 476 | 673 | 489 | 879 | 825 | 778 | 865 | 228 | 346 | 462 | 437 | 456 | 362 | 571 | 401 | 299 | 332 | 366 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.3 | 3.6 | 4.9 | 4.6 | 3.9 | 4.1 | 4.0 | 4.4 | 3.4 | 3.9 | 3.6 | 3.9 | 4.8 | 4.8 | 4.6 | 4.8 | 6.3 | 6.2 | 7.9 | 7.4 | 6.5 |
Std Deviation (1e-4) | 0.6 | 0.5 | 1.0 | 1.3 | 1.3 | 1.2 | 1.2 | 1.0 | 0.9 | 0.9 | 0.8 | 0.9 | 1.4 | 1.7 | 1.1 | 1.6 | 2.4 | 1.7 | 8.1 | 6.5 | 4.6 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.33 | -0.27 | -0.29 | -0.16 | -0.53 | -0.34 | -0.44 | -0.33 | -0.04 | -0.05 | -0.11 | -0.05 | 0.35 | 0.34 | 0.69 | 0.59 | 0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 7.1 | 5.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.8505 | 3.5693 | 3.1793 | 3.6025 | 3.5196 | 3.4988 | 7.2001 | 5.0867 | 5.2699 | 3.9599 | 5.7807 | 5.7527 | 2.4968 | 2.7099 | 2.3548 | 2.4409 | 2.1868 | 2.5209 |
Actin | 0.0713 | 0.0006 | 0.0201 | 0.0668 | 0.0006 | 0.0025 | 0.0842 | 0.0016 | 0.0207 | 0.0038 | 0.0057 | 0.0018 | 0.0068 | 0.0002 | 0.0074 | 0.0002 | 0.0003 | 0.0007 |
Bud | 0.0007 | 0.0002 | 0.0002 | 0.0016 | 0.0022 | 0.0003 | 0.0061 | 0.0046 | 0.0009 | 0.0146 | 0.0004 | 0.0004 | 0.001 | 0.0077 | 0.001 | 0 | 0.0005 | 0.0002 |
Bud Neck | 0.005 | 0.0009 | 0.0022 | 0.0024 | 0.0012 | 0.0018 | 0.0058 | 0.0058 | 0.0015 | 0.0023 | 0.0026 | 0.0064 | 0.0063 | 0.0003 | 0.0021 | 0.0003 | 0.0018 | 0.0029 |
Bud Periphery | 0.0012 | 0.0002 | 0.0003 | 0.0051 | 0.0007 | 0.0004 | 0.0041 | 0.0073 | 0.0007 | 0.0214 | 0.0008 | 0.0009 | 0.0009 | 0.0021 | 0.0036 | 0 | 0.0005 | 0.0002 |
Bud Site | 0.0097 | 0.0011 | 0.0009 | 0.0019 | 0.0004 | 0.0001 | 0.0216 | 0.0147 | 0.0034 | 0.0043 | 0.0014 | 0.0003 | 0.0079 | 0.0118 | 0.0014 | 0.0001 | 0.0002 | 0.0002 |
Cell Periphery | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0005 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.1249 | 0.166 | 0.1992 | 0.144 | 0.1644 | 0.2411 | 0.1332 | 0.2141 | 0.1809 | 0.2366 | 0.151 | 0.218 | 0.1325 | 0.1154 | 0.1248 | 0.1288 | 0.124 | 0.1597 |
Cytoplasmic Foci | 0.0266 | 0.0106 | 0.0114 | 0.0067 | 0.005 | 0.0074 | 0.0299 | 0.0302 | 0.0093 | 0.0036 | 0.0066 | 0.0201 | 0.0161 | 0.0035 | 0.0046 | 0.0053 | 0.0033 | 0.0123 |
Eisosomes | 0.0013 | 0.0003 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0077 | 0.0021 | 0.0059 | 0.0041 | 0.0025 | 0.0025 | 0.0141 | 0.0066 | 0.0022 | 0.0026 | 0.003 | 0.0037 | 0.0029 | 0.0016 | 0.002 | 0.0007 | 0.0008 | 0.0014 |
Endosome | 0.0175 | 0.0015 | 0.0087 | 0.0102 | 0.0024 | 0.0041 | 0.025 | 0.0033 | 0.0034 | 0.0017 | 0.0211 | 0.0053 | 0.0044 | 0.0012 | 0.0026 | 0.0015 | 0.0015 | 0.0026 |
Golgi | 0.0063 | 0.0001 | 0.0027 | 0.0023 | 0.0001 | 0.0005 | 0.0068 | 0.0005 | 0.0029 | 0.0001 | 0.0034 | 0.0004 | 0.0009 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0001 |
Lipid Particles | 0.0134 | 0.0004 | 0.0032 | 0.0025 | 0.0003 | 0.0013 | 0.0192 | 0.001 | 0.0007 | 0.0002 | 0.0167 | 0.001 | 0.0018 | 0.0004 | 0.003 | 0.0001 | 0.0002 | 0.0003 |
Mitochondria | 0.0044 | 0.0006 | 0.002 | 0.0025 | 0.001 | 0.0007 | 0.0033 | 0.0015 | 0.0019 | 0.0019 | 0.0042 | 0.0014 | 0.0014 | 0.0006 | 0.0044 | 0.0006 | 0.0009 | 0.0006 |
None | 0.4217 | 0.6821 | 0.4832 | 0.4431 | 0.6309 | 0.4896 | 0.3965 | 0.5669 | 0.5807 | 0.3502 | 0.4592 | 0.5408 | 0.5897 | 0.7569 | 0.634 | 0.59 | 0.5525 | 0.5876 |
Nuclear Periphery | 0.0233 | 0.0035 | 0.015 | 0.03 | 0.0058 | 0.0094 | 0.047 | 0.0066 | 0.0084 | 0.0077 | 0.0659 | 0.0116 | 0.0085 | 0.0034 | 0.0072 | 0.0066 | 0.0041 | 0.0061 |
Nucleolus | 0.0047 | 0.0021 | 0.002 | 0.0031 | 0.0024 | 0.0027 | 0.0063 | 0.0022 | 0.0026 | 0.004 | 0.0121 | 0.0018 | 0.0034 | 0.0022 | 0.0025 | 0.0028 | 0.0066 | 0.0031 |
Nucleus | 0.2087 | 0.1099 | 0.1915 | 0.2169 | 0.1613 | 0.2022 | 0.1371 | 0.0895 | 0.1108 | 0.322 | 0.2035 | 0.1352 | 0.1739 | 0.0771 | 0.1659 | 0.205 | 0.2566 | 0.163 |
Peroxisomes | 0.0112 | 0.0005 | 0.0076 | 0.0028 | 0.0002 | 0.0004 | 0.0163 | 0.0013 | 0.0009 | 0.0005 | 0.007 | 0.0005 | 0.0056 | 0.0002 | 0.0044 | 0.0001 | 0.0002 | 0.0006 |
Punctate Nuclear | 0.0363 | 0.0156 | 0.0417 | 0.0508 | 0.0174 | 0.0313 | 0.0347 | 0.0372 | 0.0666 | 0.0196 | 0.029 | 0.0469 | 0.0337 | 0.0139 | 0.0265 | 0.0572 | 0.0449 | 0.0571 |
Vacuole | 0.0023 | 0.0014 | 0.0014 | 0.0014 | 0.0011 | 0.001 | 0.0052 | 0.0035 | 0.0009 | 0.0019 | 0.0031 | 0.0026 | 0.0015 | 0.0012 | 0.001 | 0.0004 | 0.0008 | 0.0011 |
Vacuole Periphery | 0.001 | 0.0002 | 0.0004 | 0.001 | 0.0002 | 0.0003 | 0.0027 | 0.0006 | 0.0003 | 0.0006 | 0.0032 | 0.0007 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.7065 | 4.504 | 5.0132 | 6.9595 | 8.1656 | 7.5534 | 10.5311 | 6.9157 | 8.6237 | 8.5717 |
Translational Efficiency | 1.1038 | 2.0571 | 1.4306 | 0.7434 | 0.913 | 0.9273 | 0.506 | 0.4771 | 0.5956 | 0.8885 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|