Standard name | |
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Human Ortholog | |
Description | Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 24 | 47 | 41 | 12 | 23 | 23 | 30 | 52 | 44 | 42 | 25 | 23 | 38 | 42 | 42 | 38 | 27 | 42 | 0 | 1 | 0 | 0 | 1 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 60 | 63 | 86 | 68 | 98 | 178 | 276 | 177 | 175 | 243 | 218 | 228 | 28 | 21 | 28 | 31 | 15 | 9 | 0 | 0 | 1 | 0 | 2 | 1 |
Nucleus | 175 | 207 | 323 | 246 | 381 | 343 | 640 | 589 | 418 | 532 | 449 | 509 | 197 | 286 | 244 | 187 | 150 | 93 | 146 | 176 | 291 | 166 | 259 | 231 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 1 | 3 | 5 |
Nucleolus | 22 | 24 | 54 | 66 | 118 | 213 | 402 | 389 | 251 | 413 | 331 | 371 | 23 | 41 | 69 | 20 | 11 | 9 | 6 | 6 | 20 | 7 | 18 | 27 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | 0 | 0 | 1 | 5 | 1 | 3 | 7 | 6 | 3 | 6 | 3 | 0 | 2 | 6 | 5 | 8 | 4 | 2 | 2 | 7 | 8 | 17 | 46 |
Unique Cell Count | 185 | 226 | 364 | 275 | 447 | 452 | 851 | 851 | 602 | 800 | 658 | 754 | 223 | 337 | 329 | 230 | 180 | 126 | 164 | 197 | 340 | 190 | 313 | 333 |
Labelled Cell Count | 282 | 342 | 504 | 394 | 625 | 758 | 1352 | 1218 | 896 | 1234 | 1032 | 1136 | 286 | 395 | 395 | 283 | 214 | 159 | 164 | 197 | 340 | 190 | 313 | 333 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 123.5 | 125.1 | 126.5 | 110.5 | 94.5 | 72.2 | 62.3 | 62.2 | 56.6 | 49.8 | 45.5 | 44.5 | 131.2 | 122.5 | 111.6 | 167.8 | 161.9 | 177.1 | 116.5 | 128.8 | 138.7 |
Std Deviation (1e-4) | 37.2 | 42.2 | 51.9 | 38.1 | 40.7 | 28.3 | 24.5 | 29.0 | 24.5 | 22.3 | 20.2 | 19.4 | 56.7 | 63.6 | 56.2 | 68.6 | 75.9 | 88.0 | 48.1 | 53.6 | 60.3 |
Intensity Change (Log2) | – | – | – | -0.2 | -0.42 | -0.81 | -1.02 | -1.02 | -1.16 | -1.35 | -1.48 | -1.51 | 0.05 | -0.05 | -0.18 | 0.41 | 0.36 | 0.48 | -0.12 | 0.03 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.1 | -3.2 | -3.3 | -5.3 | -3.1 | -2.1 | -3.7 | -4.6 | -5.5 | 2.0 | 0.5 | 0.6 | 1.8 | 1.2 | 5.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.3 | -0.6 | 4.8 | 3.1 | -1.1 | 1.8 | 2.4 | 3.2 | 2.3 | -3.3 | -6.4 | -5.4 | -3.0 | -4.3 | -4.0 |
Nucleus | 0.3 | -1.5 | -4.7 | -5.3 | -7.2 | -6.9 | -8.0 | -7.3 | -7.6 | -0.1 | -1.5 | -5.0 | -2.5 | -1.8 | -4.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.9 | 4.0 | 9.8 | 10.7 | 10.2 | 8.7 | 11.9 | 11.2 | 11.1 | -1.6 | -1.0 | 2.1 | -2.2 | -3.0 | -2.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 191.5251 | 181.4523 | 182.3865 | 159.3778 | 157.2807 | 163.6701 | 185.7691 | 226.1555 | 201.2919 | 194.9701 | 201.2373 | 203.5379 | 199.2887 | 206.4551 | 191.6894 | 190.8351 | 183.4769 | 185.5389 |
Actin | 0.0046 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0095 | 0.0001 | 0 | 0 | 0.0101 | 0 | 0 | 0 | 0 | 0.0007 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0003 | 0 | 0 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Periphery | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0.0004 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0002 | 0 | 0.0002 | 0.002 | 0.0001 | 0.0013 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 |
Cytoplasm | 0.0006 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0023 | 0.0001 | 0.0005 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0003 | 0.0004 | 0.0114 | 0.0003 | 0 | 0 | 0.0058 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Eisosomes | 0.0001 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0017 | 0 | 0 | 0 | 0 | 0 | 0.0007 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Endosome | 0.0028 | 0 | 0 | 0 | 0 | 0 | 0.003 | 0 | 0.0014 | 0.0001 | 0 | 0 | 0.0033 | 0 | 0 | 0 | 0 | 0.0011 |
Golgi | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0017 | 0 | 0 | 0 | 0.0017 | 0 | 0 | 0 | 0 | 0.0004 |
Lipid Particles | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0019 | 0.0001 | 0 | 0 | 0.007 | 0 | 0 | 0 | 0 | 0.0009 |
Mitochondria | 0.0026 | 0.0025 | 0.0008 | 0.0171 | 0.0062 | 0.0024 | 0.0075 | 0.0025 | 0.0083 | 0.0118 | 0.0136 | 0.0011 | 0.0082 | 0.0009 | 0.0018 | 0.001 | 0.0129 | 0.0014 |
None | 0.001 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0.0009 | 0.0003 | 0.0016 | 0.0001 | 0 | 0 | 0.0008 | 0 | 0 | 0 | 0 | 0.0001 |
Nuclear Periphery | 0.0021 | 0.001 | 0.0002 | 0.0005 | 0.0001 | 0 | 0.0281 | 0.0004 | 0.0055 | 0.002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0009 |
Nucleolus | 0.062 | 0.0562 | 0.0271 | 0.067 | 0.176 | 0.075 | 0.0806 | 0.0299 | 0.0495 | 0.0678 | 0.2025 | 0.0758 | 0.0845 | 0.0443 | 0.042 | 0.051 | 0.2497 | 0.0644 |
Nucleus | 0.9201 | 0.9389 | 0.9719 | 0.9127 | 0.8105 | 0.9219 | 0.8689 | 0.9652 | 0.8971 | 0.9153 | 0.7825 | 0.922 | 0.8679 | 0.9545 | 0.9555 | 0.9478 | 0.7351 | 0.9288 |
Peroxisomes | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0025 | 0 | 0 | 0 | 0.0076 | 0 | 0 | 0 | 0 | 0.0001 |
Punctate Nuclear | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.001 | 0 | 0.0058 | 0 | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0 | 0.0003 |
Vacuole | 0.0002 | 0.0002 | 0 | 0.0002 | 0.0021 | 0.0001 | 0.0014 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 |
Vacuole Periphery | 0.0003 | 0.0004 | 0.0001 | 0.0008 | 0.002 | 0.0003 | 0.0017 | 0.0006 | 0.0011 | 0.0014 | 0.0009 | 0.0005 | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0015 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 672.6347 | 624.0339 | 652.8207 | 632.214 | 544.4544 | 820.2184 | 790.7731 | 735.7074 | 743.5 | 690.9137 |
Translational Efficiency | 1.0691 | 1.0794 | 0.9726 | 0.9566 | 1.0298 | 1.1357 | 1.1228 | 1.0344 | 0.9816 | 1.0136 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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