Standard name
Human Ortholog
Description Catalytic subunit of protein phosphatase 2A, PP2A; functionally redundant with Pph22p; C-terminally methylated; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.94 0.89 0.56 0.64 0.69 0.66 0.55 0.63 0.59 0.6 0.63 0.79 0.86 0.92 0.49 0.44 0.48 0.85 0.82 0.8 0.79 0.63 0.52
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0 0 0.08 0.13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.2 0.14 0.27 0.19 0.29 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.08 0.05 0 0.05 0.06 0 0 0 0 0 0 0 0.09 0.06 0.1 0.06 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.09
Vac/Vac Membrane 0 0.08 0.12 0.52 0.41 0.24 0.23 0.31 0.19 0.29 0.17 0.27 0.31 0.29 0.16 0.56 0.6 0.46 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 1 0 1 0 1 0 0 0 1 0 0 1 1 0 0 1 3
Bud 0 0 0 0 0 1 5 4 3 3 5 2 1 0 0 0 0 0 0 0 1 1 6 10
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 4 0 2 1 3 6 3 5 0 0 0 2 5 0 0 0 0 0 0 0
Cytoplasm 123 160 102 97 172 167 202 185 139 152 132 155 229 297 335 148 100 105 114 150 134 185 190 157
Endoplasmic Reticulum 1 2 1 0 2 0 1 3 3 3 0 3 1 0 0 9 4 4 1 1 2 0 2 3
Endosome 0 0 2 2 5 2 0 2 0 1 1 1 3 1 2 15 14 13 1 2 4 2 25 39
Golgi 1 0 0 0 0 0 1 1 0 0 0 0 2 1 0 7 5 6 2 0 0 1 12 5
Mitochondria 4 1 0 4 7 26 60 46 59 48 65 30 0 0 0 1 3 9 1 1 3 3 6 4
Nucleus 0 1 2 3 9 9 7 27 12 9 12 15 10 5 4 10 5 9 4 15 9 24 17 14
Nuclear Periphery 0 0 1 0 0 0 1 1 0 3 0 0 0 0 0 0 1 0 0 0 0 1 1 1
Nucleolus 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 12 8 9 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 4
SpindlePole 0 0 0 2 1 0 0 1 0 0 0 0 0 0 0 7 5 4 0 0 2 1 24 27
Vac/Vac Membrane 2 13 14 90 110 59 71 106 42 74 38 66 89 101 59 170 136 101 3 8 6 9 6 15
Unique Cell Count 127 170 115 173 269 243 306 339 222 259 221 247 289 346 365 301 226 218 134 184 169 236 303 300
Labelled Cell Count 131 177 122 200 311 264 350 378 261 300 256 279 335 405 400 382 286 260 134 184 169 236 303 300


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 9.8 7.6 8.4 8.4 7.3 7.0 7.4 6.8 7.2 6.8 7.6 9.0 9.5 9.6 10.9 11.9 11.5 8.7 8.6 9.2
Std Deviation (1e-4) 0.9 2.0 1.2 1.9 1.9 1.8 1.7 1.3 1.6 1.5 1.6 1.6 1.4 1.3 1.3 2.6 2.6 2.8 1.5 1.4 1.9
Intensity Change (Log2) – – – 0.14 0.14 -0.07 -0.12 -0.05 -0.15 -0.07 -0.17 -0.01 0.24 0.32 0.34 0.52 0.64 0.59 0.19 0.17 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -5.9 -4.9 -4.1 -4.6 -6.6 -5.0 -5.7 -5.5 -5.1 -2.2 -0.8 1.0 -7.4 -7.9 -7.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1.8 1.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.6 5.1 4.2 6.1 4.9 6.5 3.9 0 0 0 0 0 0
Nucleus 0 0 0 0 2.4 0 0 0 1.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 6.9 5.5 2.7 2.5 4.0 1.6 3.4 1.2 3.1 3.9 3.7 1.0 8.1 8.4 6.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.528 6.9575 6.066 5.9281 4.5333 6.0194 4.2038 5.5641 5.0511 4.4233 4.2625 5.0375 3.6237 4.9197 3.8897 3.6869 3.3094 4.1266
Actin 0.0102 0.0012 0.0013 0.0004 0.0314 0.0008 0.0311 0.0004 0.0079 0.003 0.0029 0.0034 0.0538 0.0002 0.0167 0.1251 0.0242 0.0006
Bud 0.0005 0.0002 0.0002 0.0001 0.0067 0.0001 0.0092 0.0004 0.0051 0.001 0.0003 0.0002 0.001 0.0001 0.0008 0.0009 0.0002 0.0014
Bud Neck 0.0023 0.0006 0.0011 0.0007 0.0043 0.0021 0.0015 0.0008 0.0028 0.0017 0.0012 0.0024 0.0041 0.0003 0.001 0.0031 0.0009 0.0025
Bud Periphery 0.0003 0.0001 0.0001 0 0.0032 0.0001 0.0034 0.0001 0.0019 0.0003 0.0003 0.0001 0.002 0 0.0009 0.0006 0.0002 0.001
Bud Site 0.0049 0.0016 0.0006 0.0001 0.0039 0.0002 0.0037 0.001 0.0023 0.0008 0.0008 0.0002 0.0058 0.0005 0.0145 0.002 0.0006 0.0005
Cell Periphery 0.0004 0 0 0 0.0003 0 0.0003 0 0.0002 0.0001 0.0001 0 0.0006 0 0.0003 0.0002 0 0.0001
Cytoplasm 0.4974 0.5262 0.4337 0.3189 0.4728 0.497 0.5131 0.5882 0.5442 0.3688 0.5397 0.518 0.6127 0.7442 0.559 0.4914 0.3438 0.6643
Cytoplasmic Foci 0.0186 0.0025 0.0105 0.0019 0.0356 0.0066 0.0185 0.0036 0.0127 0.0394 0.0376 0.0057 0.0099 0.0013 0.0235 0.0048 0.0282 0.0055
Eisosomes 0.0001 0 0 0 0.0003 0 0.0002 0 0 0 0 0 0.0004 0 0.0002 0.0004 0.0001 0
Endoplasmic Reticulum 0.0094 0.0015 0.0031 0.001 0.0049 0.0027 0.0095 0.0025 0.005 0.0042 0.0027 0.0031 0.0034 0.0019 0.0036 0.0015 0.0013 0.0053
Endosome 0.0337 0.0013 0.0068 0.001 0.0236 0.0069 0.032 0.0031 0.0124 0.0405 0.0264 0.0088 0.0126 0.0008 0.0264 0.0026 0.0066 0.0135
Golgi 0.0068 0.0001 0.0012 0 0.0067 0.0007 0.0086 0.0001 0.0021 0.0039 0.0247 0.0013 0.0116 0 0.0265 0.0066 0.02 0.0009
Lipid Particles 0.011 0 0.0022 0 0.011 0.0004 0.0103 0 0.0028 0.0082 0.0315 0.0034 0.038 0 0.0278 0.0016 0.0509 0
Mitochondria 0.0063 0.0003 0.0124 0.0002 0.0149 0.0043 0.012 0.0009 0.0053 0.0014 0.0056 0.0011 0.0287 0.0002 0.0293 0.0015 0.0046 0.0015
None 0.0264 0.0137 0.0118 0.0664 0.0345 0.0089 0.0067 0.0072 0.0043 0.0043 0.0054 0.0029 0.0023 0.0022 0.0021 0.0026 0.0006 0.0026
Nuclear Periphery 0.0169 0.0104 0.0144 0.0087 0.0193 0.0126 0.023 0.0106 0.0137 0.0163 0.0123 0.0171 0.0193 0.0091 0.0185 0.0107 0.0173 0.0219
Nucleolus 0.0008 0.0004 0.0005 0.0002 0.003 0.0004 0.0006 0.0003 0.0024 0.0015 0.0006 0.0004 0.0004 0.0001 0.0009 0.0014 0.0002 0.0002
Nucleus 0.3296 0.4344 0.4801 0.5956 0.2881 0.4414 0.298 0.3737 0.359 0.4622 0.2362 0.4203 0.1679 0.2368 0.2087 0.2777 0.4316 0.2665
Peroxisomes 0.005 0.0003 0.0081 0.0002 0.0094 0.0021 0.0046 0.0003 0.0014 0.0086 0.0267 0.0016 0.0132 0 0.0215 0.0014 0.0644 0.0002
Punctate Nuclear 0.0158 0.0038 0.0102 0.0041 0.0224 0.0107 0.0092 0.0051 0.0108 0.0307 0.0426 0.0078 0.0028 0.0016 0.0066 0.0631 0.0034 0.0046
Vacuole 0.0025 0.001 0.0013 0.0003 0.0029 0.0015 0.003 0.0014 0.0027 0.0023 0.0017 0.0016 0.0063 0.0005 0.0086 0.0008 0.0007 0.0055
Vacuole Periphery 0.0009 0.0002 0.0003 0.0001 0.0009 0.0003 0.0015 0.0002 0.0009 0.0007 0.0007 0.0006 0.0031 0.0001 0.0025 0.0003 0.0003 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 67.0989 61.3153 52.0926 75.1968 62.5087 67.5585 76.147 77.925 85.4675 66.537
Translational Efficiency 1.079 1.1616 1.3931 0.944 0.9433 1.1988 0.963 0.9871 0.8812 0.9703

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Catalytic subunit of protein phosphatase 2A, PP2A; functionally redundant with Pph22p; C-terminally methylated; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (89%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pph21

Pph21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pph21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available