Standard name
Human Ortholog
Description Subunit of the CSN and 26S proteasome lid complexes; similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein; the COP9 signalosome is also known as the CSN
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.1 0.08 0.11 0.09 0.08 0.07 0.06 0.06 0.05 0.12 0.23 0 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.07 0.09 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.83 0.76 0.84 0.66 0.55 0.43 0.48 0.51 0.54 0.5 0.45 0.48 0.76 0.6 0.54 0.63 0.57 0.5 0.75 0.85 0.59 0.88 0.79 0.71
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.19 0.06 0.08 0.14 0.05 0.08 0.11 0.09 0.12 0.1 0.16 0.11 0 0 0 0.06 0 0 0.17 0.07 0.26 0 0.07 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.22 0.07 0.16 0.33 0.43 0.32 0.31 0.28 0.28 0.3 0.31 0.16 0.25 0.21 0.31 0.36 0.4 0.05 0.07 0.08 0.05 0.09 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 3 1 0 5 1 0 0 0 0 0 0 0 0 1 1 1 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 2 0 4 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 3 6 1 13 12 20 19 12 17 9 11 9 20 45 3 13 9 0 0 0 1 3 2
Endoplasmic Reticulum 0 0 0 0 0 1 1 0 0 1 0 1 0 0 0 6 3 2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 1 1 0 0 0 2 0 1 0 0 0 2 0 2 0
Golgi 0 0 0 1 1 0 0 2 2 1 0 0 0 6 3 4 2 2 0 0 0 0 0 1
Mitochondria 1 0 0 1 0 2 5 1 3 16 13 9 0 0 0 1 0 0 0 0 0 0 1 1
Nucleus 30 105 118 38 72 61 85 107 86 116 64 85 129 104 107 90 110 64 49 135 111 150 211 238
Nuclear Periphery 0 0 2 2 0 0 0 4 0 1 2 1 0 0 3 0 0 1 0 0 6 0 0 0
Nucleolus 7 8 11 8 7 12 19 19 19 24 23 20 5 5 6 9 6 5 11 10 49 6 18 26
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0
Vac/Vac Membrane 3 31 10 9 43 61 56 65 44 65 42 54 27 43 43 44 70 51 3 11 15 9 22 54
Unique Cell Count 36 139 141 58 131 142 177 211 159 230 142 176 169 173 200 143 192 128 66 160 191 172 268 336
Labelled Cell Count 43 148 147 62 136 149 190 220 166 252 156 183 170 178 209 157 205 134 66 160 191 172 268 336


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 26.6 42.4 32.8 29.0 27.8 27.1 24.4 24.9 23.9 22.3 21.1 22.3 33.6 32.7 29.6 39.7 38.2 43.7 30.0 31.3 34.5
Std Deviation (1e-4) 6.3 8.6 7.6 7.0 7.0 6.9 6.6 6.6 7.0 6.1 6.2 6.0 8.6 9.7 9.5 12.7 12.0 13.7 7.9 9.7 9.2
Intensity Change (Log2) – – – -0.18 -0.24 -0.28 -0.43 -0.4 -0.46 -0.56 -0.64 -0.56 0.03 -0.0 -0.15 0.28 0.22 0.41 -0.13 -0.07 0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.8 1.4 2.3 1.7 1.2 1.2 0.8 0.8 0.4 2.3 4.7 0 1.0 1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.2 3.7 0 0 0 0 0 0 0
Nucleus -2.8 -5.2 -7.1 -6.6 -6.3 -5.5 -6.4 -6.8 -6.5 -1.6 -4.6 -5.8 -3.9 -5.1 -5.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.3 -0.8 0.2 0.9 0.4 1.2 0.8 2.2 1.1 -1.9 -2.0 -2.0 -0.5 -1.9 -1.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.8 5.4 7.0 5.4 5.3 4.6 4.9 4.9 5.2 2.4 4.2 3.6 5.1 6.2 6.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 28.5403 33.1078 28.4447 25.5225 25.9402 28.9971 29.0501 38.3635 33.8228 33.2198 29.8899 33.909 29.4981 33.3104 31.5181 30.2504 26.5478 30.5078
Actin 0.0003 0.0004 0.0037 0.0001 0.0231 0.0003 0.0164 0.0003 0.0001 0.0004 0.0011 0.0038 0.0145 0 0.0014 0.0001 0.0351 0.0001
Bud 0.0004 0.0001 0.0002 0.0001 0.0005 0.0001 0.0006 0 0.0025 0.0001 0.0003 0.0001 0.0001 0 0 0.0001 0.0002 0
Bud Neck 0.0003 0.0001 0.0001 0 0.0004 0.0007 0.0003 0 0.0001 0.0001 0.0001 0.0007 0.0004 0 0.0001 0 0.0003 0.0003
Bud Periphery 0.0005 0.0001 0.0004 0 0.0005 0.0001 0.0005 0 0.0009 0.0001 0.0008 0.0002 0.0002 0 0 0.0001 0.0004 0.0001
Bud Site 0.0008 0.0001 0.0003 0.0004 0.0026 0 0.0011 0.0002 0.0002 0.0002 0.0002 0.0001 0.0005 0 0.0001 0 0.0005 0
Cell Periphery 0.0007 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0 0.0002 0.0003 0.0002 0.0001 0.0002 0 0 0 0.0002 0.0001
Cytoplasm 0.0045 0.0019 0.0004 0.0006 0.0006 0.0011 0.0091 0.0005 0.0089 0.003 0.0009 0.0015 0.0075 0.0002 0.0005 0.0005 0.0002 0.0006
Cytoplasmic Foci 0.0143 0 0.009 0.0298 0.0077 0.0044 0.0072 0.0023 0.0039 0.0011 0.0026 0.0024 0.0118 0 0.0001 0 0.0025 0.0075
Eisosomes 0 0 0 0 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0007 0.0001 0.0001 0 0.0004 0.0008 0.0059 0 0.0001 0.0003 0.0008 0.0005 0.0088 0 0.0015 0 0.0001 0
Endosome 0.0055 0 0.0023 0.0005 0.0045 0.003 0.0225 0.0001 0.0002 0.0003 0.0146 0.0046 0.0089 0 0.0025 0 0.0011 0.0003
Golgi 0.0005 0 0.0014 0.0001 0.0034 0.0002 0.0062 0.0001 0.0001 0.0001 0.003 0.0021 0.0015 0 0.0002 0 0.0022 0.0001
Lipid Particles 0.0074 0 0.0031 0.0072 0.0081 0.0023 0.0035 0.0016 0.001 0.0005 0.003 0.0013 0.0063 0 0.0002 0 0.0035 0.0026
Mitochondria 0.005 0.0006 0.0092 0.0017 0.0037 0.001 0.0068 0.0008 0.0029 0.0034 0.0255 0.003 0.0039 0.0004 0.001 0.0015 0.0013 0.0009
None 0.0025 0 0.001 0.0011 0.0002 0.0003 0.0042 0.0001 0.0012 0.0011 0.0019 0.0002 0.0031 0 0.0002 0 0.0002 0.0004
Nuclear Periphery 0.0212 0.0249 0.0087 0.0072 0.0308 0.0091 0.0482 0.0169 0.0073 0.0165 0.0207 0.0135 0.0134 0.0099 0.0141 0.0035 0.0132 0.0068
Nucleolus 0.0361 0.0103 0.0135 0.0174 0.0235 0.0293 0.0215 0.0068 0.0141 0.0192 0.0468 0.0277 0.0219 0.0091 0.0033 0.0033 0.0549 0.0162
Nucleus 0.8788 0.9592 0.9374 0.929 0.8735 0.944 0.819 0.9687 0.9499 0.944 0.8626 0.9337 0.8857 0.9801 0.9738 0.9904 0.8759 0.9624
Peroxisomes 0.0005 0 0.0045 0.0006 0.0074 0.0002 0.0012 0.0003 0.0001 0.0001 0.0005 0.0007 0.0007 0 0 0 0.0061 0.0001
Punctate Nuclear 0.0082 0.0003 0.0015 0.0015 0.0029 0.0007 0.0176 0.0004 0.0039 0.0018 0.0018 0.0013 0.0055 0 0.0003 0.0001 0.0007 0.0005
Vacuole 0.0066 0.0005 0.0011 0.0014 0.0017 0.001 0.0037 0.0002 0.001 0.0027 0.0024 0.0008 0.0026 0.0001 0.0002 0.0001 0.0007 0.0005
Vacuole Periphery 0.0051 0.0011 0.0018 0.0011 0.0044 0.0013 0.0041 0.0004 0.0012 0.0047 0.0102 0.0015 0.0024 0.0002 0.0005 0.0002 0.0007 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 115.8657 108.3687 99.5605 130.1374 101.041 100.5537 117.5843 117.9732 122.3743 115.9658
Translational Efficiency 2.1586 1.8826 2.0451 1.6001 1.7235 2.6023 1.7266 1.7279 1.6759 1.599

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the CSN and 26S proteasome lid complexes; similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein; the COP9 signalosome is also known as the CSN
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Rpn5

Rpn5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpn5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available