Standard name
Human Ortholog
Description Nuclear protein with a role in protein quality control; localizes to the intranuclear quality control compartment (INQ) in response to proteasome inhibition or DNA replication stress; INQ likely sequesters proteins involved in DNA metabolism for degradation or re-folding; DNA-binding protein with preference for UV-damaged DNA; contains three WD domains (WD-40 repeat); human ortholog WDR76 also exhibits perinuclear localization under similar stress conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.25 0.19 0.36 0.25 0.29 0.31 0.29 0.19 0.21 0.23 0.14 0.11 0.47 0.62 0.6 0.45 0.39 0.35 0.15 0.13 0.15 0.31 0.46 0.41
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.12 0.05 0 0 0 0 0 0
Endosome 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.14 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.46 0.13 0.21 0.5 0.34 0.52 0.67 0.73 0.73 0.75 0.83 0.81 0 0 0 0.06 0 0 0 0.05 0.08 0 0.05 0
Nucleus 0.42 0.37 0.25 0.28 0.27 0.25 0.2 0.3 0.28 0.3 0.26 0.32 0.26 0.12 0.08 0.27 0.24 0.21 0.46 0.43 0.31 0.11 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0.05 0 0 0.06 0.07 0.09
Vac/Vac Membrane 0.15 0.33 0.23 0.2 0.24 0.18 0.12 0 0.08 0.09 0.07 0.11 0.37 0.36 0.44 0.21 0.32 0.37 0.24 0.27 0.28 0.33 0.26 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 1 0 2
Bud 0 1 0 1 1 1 1 1 6 3 4 2 0 0 0 0 0 0 6 7 5 4 3 4
Bud Neck 0 2 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 1 1 2 0 3
Bud Site 0 0 0 0 0 0 1 1 6 5 3 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 62 66 43 24 40 44 58 29 48 58 34 23 74 130 170 181 72 73 47 64 24 38 76 84
Endoplasmic Reticulum 1 2 2 0 0 0 1 1 0 0 1 0 1 1 2 28 22 10 1 3 2 2 2 1
Endosome 0 35 4 4 3 0 1 1 0 0 0 0 5 6 6 33 19 30 2 5 2 4 6 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 2 0 3 1 1 0 5
Mitochondria 116 44 25 48 47 74 135 112 164 193 200 168 1 5 1 22 2 6 6 21 13 2 8 6
Nucleus 107 128 30 27 37 35 40 46 62 78 62 67 41 25 23 106 45 44 142 209 50 13 6 5
Nuclear Periphery 0 3 0 0 0 0 0 3 1 1 1 3 0 0 0 0 0 1 0 0 0 0 1 0
Nucleolus 0 0 0 1 0 1 2 1 1 1 1 0 0 0 1 3 3 6 2 2 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 1 0 0 1 1 1 1 1 2 2 0 1 20 12 15 16 19 6 7 12 18
Vac/Vac Membrane 38 114 27 19 33 25 25 5 17 24 16 22 57 77 123 82 59 76 74 131 44 40 42 62
Unique Cell Count 253 343 118 96 139 142 202 154 225 256 240 207 156 211 281 399 185 208 312 481 161 124 167 205
Labelled Cell Count 324 396 132 125 161 180 265 201 306 364 323 292 184 244 330 477 235 263 312 481 161 124 167 205


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.4 4.5 3.8 4.2 3.8 3.3 3.5 3.6 3.4 3.3 3.2 5.1 4.8 5.0 5.6 6.7 6.2 4.5 4.3 5.2
Std Deviation (1e-4) 0.5 0.7 1.6 1.1 1.9 1.8 1.5 1.5 1.8 1.8 1.3 1.3 1.5 1.5 1.7 1.6 1.9 1.9 1.4 1.3 1.7
Intensity Change (Log2) – – – -0.23 -0.09 -0.24 -0.45 -0.35 -0.33 -0.41 -0.45 -0.48 0.2 0.11 0.17 0.33 0.58 0.47 0.02 -0.05 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – -0.5 1.7 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8047 2.0733 2.1448 1.9999 1.6337 2.2292 2.471 2.9657 3.078 3.2779 2.3094 3.0507 5.1172 3.3208 3.7842 3.7529 4.3412 3.4463
Actin 0.0597 0.0238 0.0022 0.0002 0.0003 0.0007 0.0004 0.0003 0.0001 0.0358 0.0005 0.0051 0.0656 0.0001 0.0114 0.0041 0.0008 0.0002
Bud 0.0008 0.0003 0.0002 0.0001 0 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0013 0.0001 0.0001 0.003 0.0001 0.0001
Bud Neck 0.0041 0.0009 0.0003 0.0013 0.0016 0.0032 0.0008 0.0005 0.0004 0.0005 0.0008 0.0063 0.024 0.0005 0.0005 0.0115 0.0007 0.0026
Bud Periphery 0.0012 0.0002 0.0002 0.0001 0 0.0003 0.0003 0 0 0.0001 0.0001 0.0002 0.0034 0.0001 0.0001 0.0041 0.0001 0.0001
Bud Site 0.0037 0.0024 0.0012 0.0005 0.0001 0.0005 0.0006 0.001 0.0001 0.0004 0.0002 0.0003 0.0086 0.0006 0.0003 0.0133 0.0003 0.0002
Cell Periphery 0.0004 0.0002 0.0001 0 0 0.0001 0.0001 0 0 0 0 0 0.0006 0 0 0.0002 0.0001 0
Cytoplasm 0.0542 0.0987 0.0086 0.0296 0.038 0.05 0.0321 0.0391 0.0149 0.025 0.0418 0.0255 0.0596 0.0326 0.0075 0.0282 0.0287 0.0387
Cytoplasmic Foci 0.0437 0.0026 0.0017 0.0004 0.0017 0.0029 0.0087 0.0012 0.0007 0.0088 0.0116 0.0019 0.0301 0.0007 0.0008 0.0059 0.0117 0.0018
Eisosomes 0.0005 0.0016 0.0002 0 0 0.0001 0 0 0 0.0003 0 0 0.0011 0 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.0155 0.003 0.001 0.0007 0.0008 0.0023 0.0014 0.001 0.0003 0.0016 0.0008 0.0008 0.0124 0.0008 0.0003 0.0017 0.0019 0.0006
Endosome 0.0423 0.0026 0.0052 0.0007 0.0021 0.004 0.0039 0.0009 0.0004 0.0031 0.0023 0.0012 0.0329 0.0006 0.0022 0.0023 0.0043 0.001
Golgi 0.0114 0.0005 0.0044 0 0 0.0002 0.0005 0 0 0.0016 0.0002 0.0002 0.0098 0 0.0023 0.0009 0.0002 0
Lipid Particles 0.0217 0.0009 0.0007 0.0001 0.0002 0.0011 0.0041 0.0001 0 0.0016 0.0003 0.0003 0.0268 0.0001 0.0027 0.0007 0.0021 0.0004
Mitochondria 0.0052 0.0016 0.0025 0.0007 0.0009 0.0008 0.0022 0.0004 0.0002 0.001 0.0008 0.0002 0.0038 0.0004 0.01 0.0016 0.0009 0.0003
None 0.2022 0.2008 0.0959 0.095 0.1552 0.1041 0.0782 0.103 0.0647 0.1491 0.0909 0.0851 0.0954 0.0663 0.0307 0.1201 0.2638 0.0994
Nuclear Periphery 0.0318 0.027 0.0163 0.0093 0.0151 0.0091 0.01 0.0074 0.006 0.0112 0.0116 0.0075 0.047 0.0096 0.0047 0.0104 0.0167 0.0057
Nucleolus 0.014 0.0152 0.0071 0.0072 0.0177 0.0158 0.0075 0.0075 0.0056 0.0066 0.0092 0.0108 0.0115 0.0084 0.0062 0.0112 0.0104 0.0115
Nucleus 0.374 0.5912 0.8417 0.7931 0.7135 0.7404 0.7781 0.7809 0.8626 0.6455 0.6738 0.745 0.4916 0.8302 0.8992 0.734 0.5608 0.7914
Peroxisomes 0.0538 0.0028 0.0002 0 0.0001 0.0008 0.0023 0.0001 0 0.0017 0.0012 0.0007 0.0081 0 0.0021 0.0019 0.0005 0.0001
Punctate Nuclear 0.055 0.0207 0.0086 0.0602 0.0517 0.0609 0.066 0.0558 0.0435 0.1054 0.1528 0.1082 0.0572 0.0481 0.0182 0.0434 0.0933 0.0453
Vacuole 0.0032 0.0024 0.0009 0.0007 0.0006 0.0021 0.002 0.0005 0.0002 0.0003 0.0008 0.0005 0.0068 0.0006 0.0003 0.0011 0.002 0.0006
Vacuole Periphery 0.0016 0.0007 0.0005 0.0002 0.0003 0.0003 0.0006 0.0001 0.0001 0.0002 0.0003 0.0001 0.0027 0.0002 0.0002 0.0003 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.6586 5.658 7.464 13.1733 13.677 13.3544 8.899 7.0788 13.4366 16.5257
Translational Efficiency 1.2167 0.9002 0.7357 0.9873 0.8407 1.3866 0.6255 0.8662 0.7133 0.8867

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nuclear protein with a role in protein quality control; localizes to the intranuclear quality control compartment (INQ) in response to proteasome inhibition or DNA replication stress; INQ likely sequesters proteins involved in DNA metabolism for degradation or re-folding; DNA-binding protein with preference for UV-damaged DNA; contains three WD domains (WD-40 repeat); human ortholog WDR76 also exhibits perinuclear localization under similar stress conditions
Localization
Cell Percentages nucleus (75%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Cmr1

Cmr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cmr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available