Standard name
Human Ortholog
Description Transcriptional activator for GABA-dependent induction of GABA genes; binds to DNA elements found in the promoters of target genes and increases their expression in the presence of GABA (gamma-aminobutyrate); zinc finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus; examples of GABA genes include UGA1, UGA2, and UGA4
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.06 0.1 0.08 0.1 0.1 0.08 0.09 0.08 0.09 0.23 0.34 0.5 0.35 0.31 0.28 0 0 0 0.08 0.05 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.09 0.05 0 0 0 0 0 0.05 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.28 0.11 0.13 0.16 0.19 0.56 0.6 0.55 0.7 0.72 0.74 0.77 0.05 0 0 0.13 0 0.08 0 0 0 0 0 0
Nucleus 0.82 0.61 0.77 0.83 0.68 0.56 0.5 0.49 0.5 0.48 0.45 0.39 0.53 0.28 0.13 0.37 0.42 0.44 0.84 0.77 0.71 0.55 0.51 0.35
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.11
Vac/Vac Membrane 0.08 0.38 0.11 0 0.12 0.11 0.11 0.06 0.07 0.07 0.1 0.08 0.21 0.4 0.45 0.19 0.24 0.32 0.06 0.13 0.18 0.22 0.16 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3
Bud 0 0 0 0 3 1 9 9 12 10 3 4 0 0 0 0 0 0 2 4 2 3 7 19
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 7
Bud Site 1 0 0 0 0 5 9 9 13 14 4 12 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 6 5 17 12 36 40 67 71 44 58 22 35 44 141 218 75 47 22 1 4 8 13 23 20
Endoplasmic Reticulum 0 1 1 0 0 1 2 1 1 0 0 1 1 1 1 18 3 2 0 0 0 2 0 3
Endosome 0 5 3 4 11 2 8 4 0 2 0 0 9 13 21 19 8 1 1 2 4 1 23 30
Golgi 0 0 1 1 0 0 0 1 0 0 0 0 0 0 1 0 2 1 0 2 2 1 2 2
Mitochondria 41 18 37 34 66 265 405 374 374 459 215 284 9 13 5 29 5 6 0 0 2 4 14 14
Nucleus 120 104 224 176 233 264 339 334 267 307 130 143 103 114 58 80 64 34 128 145 239 93 218 129
Nuclear Periphery 0 2 0 1 1 1 6 4 4 2 1 1 1 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 2 2 0 2 1 1 3 12 7 12 1 5 0 0 0 2 2 0 3 0 2 1 11 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1
SpindlePole 1 0 1 0 1 0 3 1 0 0 0 1 0 4 0 6 1 0 5 1 4 4 43 39
Vac/Vac Membrane 11 65 32 9 40 52 78 44 37 47 30 31 41 166 198 42 36 25 9 24 60 36 70 73
Unique Cell Count 146 171 291 213 343 472 679 680 531 641 290 370 195 410 437 216 153 78 154 190 337 169 432 366
Labelled Cell Count 182 202 316 239 392 632 930 864 759 911 406 517 208 453 502 274 168 91 154 190 337 169 432 366


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.1 5.3 4.9 4.8 4.0 3.7 4.0 3.7 3.6 3.6 3.5 5.0 4.8 4.8 5.2 6.5 6.6 5.3 5.1 6.2
Std Deviation (1e-4) 0.7 0.8 1.4 1.1 1.9 1.5 1.7 1.8 1.5 1.6 1.3 1.5 1.1 1.4 1.5 1.4 1.8 1.4 1.5 1.2 1.8
Intensity Change (Log2) – – – -0.13 -0.16 -0.42 -0.52 -0.42 -0.52 -0.58 -0.59 -0.61 -0.11 -0.17 -0.14 -0.05 0.28 0.3 -0.01 -0.07 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 2.1 1.3 2.0 2.3 1.3 1.7 0.8 1.7 5.5 8.9 12.5 8.3 7.1 5.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 4.7 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 4.2 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.0 0 0 0 0 0 0 0 0 -3.0 0 0 0.2 -3.2 -1.2
Nucleus 1.5 -2.5 -5.9 -7.8 -8.0 -7.5 -8.3 -7.9 -9.8 -5.6 -12.8 -17.3 -9.1 -7.4 -5.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.7 0.3 0 0.2 -2.4 -2.0 -1.9 -0.3 -1.1 3.0 8.5 9.8 2.7 3.5 4.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3748 0.6803 0.4957 0.2241 0.1228 0.4749 1.9777 2.1108 1.5225 1.7667 1.5834 2.0196 2.5191 2.7133 2.4838 2.2859 2.4523 2.4785
Actin 0.016 0.0033 0.0039 0.001 0.0001 0.0004 0.0575 0.0001 0.0428 0.0428 0.0013 0.0129 0.0466 0.0002 0.0097 0.0003 0.0006 0.0082
Bud 0.0007 0.0003 0.0003 0.0001 0 0 0.0166 0.0001 0.0004 0.0001 0.0001 0.0005 0.0008 0.0001 0.0001 0.0001 0 0.0001
Bud Neck 0.0058 0.0003 0.0009 0.0005 0.0007 0.0026 0.0016 0.0003 0.0007 0.0012 0.0007 0.0039 0.0022 0.0007 0.0036 0.0005 0.0014 0.0018
Bud Periphery 0.0017 0.0005 0.0004 0.0007 0 0.0001 0.0037 0 0.0006 0.0002 0.0001 0.0014 0.0012 0.0001 0.0003 0.0001 0.0001 0.0001
Bud Site 0.0155 0.0017 0.0054 0.0002 0.0001 0.0001 0.0044 0.0003 0.0028 0.0008 0.0003 0.001 0.003 0.0018 0.0007 0.0002 0.0001 0.0001
Cell Periphery 0.0011 0.0001 0.0001 0.0005 0 0 0.0003 0 0.0001 0.0001 0 0.0003 0.0003 0.0001 0.0005 0 0 0
Cytoplasm 0.0442 0.0036 0.0269 0.034 0.0074 0.0293 0.026 0.0582 0.0165 0.0092 0.0275 0.0152 0.0193 0.0369 0.0245 0.0186 0.0184 0.0156
Cytoplasmic Foci 0.0134 0.0023 0.0029 0.0011 0.0001 0.0009 0.0414 0.002 0.0075 0.0058 0.0104 0.0035 0.0072 0.0009 0.0011 0.0003 0.0018 0.0007
Eisosomes 0.0005 0.0001 0.0003 0.0002 0 0.0001 0.0007 0.0004 0.0004 0.0002 0.0001 0.0001 0.0006 0 0.0018 0 0 0.0001
Endoplasmic Reticulum 0.0048 0.0003 0.002 0.0021 0.0002 0.0009 0.0059 0.0011 0.001 0.0007 0.0009 0.0012 0.0026 0.001 0.0013 0.0003 0.0006 0.001
Endosome 0.0111 0.0038 0.0098 0.0012 0.0004 0.001 0.0591 0.0014 0.0058 0.0079 0.0155 0.0073 0.008 0.001 0.003 0.0002 0.0028 0.0031
Golgi 0.0035 0.0036 0.0037 0.0002 0 0 0.0304 0 0.0057 0.0059 0.0073 0.014 0.0042 0.0001 0.0022 0 0.0035 0.0012
Lipid Particles 0.0125 0.0037 0.0044 0.0004 0 0.0002 0.0401 0.0001 0.0028 0.0146 0.0436 0.0035 0.0055 0.0001 0.0034 0 0.0166 0.001
Mitochondria 0.0031 0.0149 0.0038 0.0407 0.0002 0.0002 0.0136 0.0004 0.0102 0.0021 0.0042 0.012 0.0055 0.0003 0.0029 0.0005 0.0038 0.0011
None 0.0672 0.0003 0.0329 0.074 0.0025 0.0383 0.0467 0.0506 0.0411 0.0539 0.1034 0.0189 0.0208 0.0436 0.0437 0.0679 0.041 0.0316
Nuclear Periphery 0.0177 0.0056 0.0212 0.0121 0.0112 0.0062 0.0219 0.0187 0.006 0.0062 0.014 0.0052 0.0175 0.0079 0.0067 0.0082 0.0058 0.0074
Nucleolus 0.0086 0.0048 0.0068 0.0071 0.0074 0.0067 0.0047 0.0049 0.0038 0.0051 0.0099 0.0089 0.0072 0.006 0.0038 0.0048 0.007 0.0089
Nucleus 0.7443 0.9401 0.8519 0.8141 0.9391 0.8992 0.5598 0.8288 0.8342 0.8257 0.6615 0.8699 0.8182 0.8706 0.8826 0.8841 0.88 0.9068
Peroxisomes 0.0043 0.0046 0.003 0.0001 0 0 0.0418 0.0001 0.009 0.0026 0.0457 0.0039 0.0125 0.0001 0.0009 0 0.0016 0.0002
Punctate Nuclear 0.0187 0.0043 0.0169 0.0061 0.0301 0.0128 0.0161 0.0315 0.0075 0.014 0.0511 0.0137 0.0147 0.0271 0.0064 0.0137 0.0141 0.0102
Vacuole 0.0044 0.0013 0.0016 0.0017 0.0002 0.0007 0.0048 0.0005 0.0007 0.0007 0.002 0.0024 0.0012 0.0013 0.0005 0.0001 0.0005 0.0005
Vacuole Periphery 0.0011 0.0003 0.0009 0.0019 0.0001 0.0001 0.0029 0.0002 0.0004 0.0003 0.0006 0.0004 0.0008 0.0001 0.0003 0.0001 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.4248 17.403 9.6853 14.4293 17.1277 15.9825 14.9253 15.0573 13.727 17.9277
Translational Efficiency 0.4621 0.5182 0.7041 0.6904 0.6337 0.5669 0.6531 0.686 0.6414 0.474

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Transcriptional activator for GABA-dependent induction of GABA genes; binds to DNA elements found in the promoters of target genes and increases their expression in the presence of GABA (gamma-aminobutyrate); zinc finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus; examples of GABA genes include UGA1, UGA2, and UGA4
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Uga3

Uga3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Uga3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available