Standard name | |
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Human Ortholog | |
Description | Major mitochondrial D-lactate dehydrogenase; oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 4 | – | 5 | 4 | 1 | 1 | – | 2 | 2 | 0 | 0 | – | – | – | 9 | 8 | 3 | 5 | 1 | 2 | 0 | 0 | 0 |
Bud | 2 | 1 | – | 2 | 4 | 5 | 10 | – | 7 | 7 | 7 | 9 | – | – | – | 4 | 6 | 9 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | – | 2 | 1 | 0 | 1 | – | 2 | 3 | 0 | 1 | – | – | – | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 2 | 0 | 0 | 4 | – | 2 | 3 | 5 | 1 | – | – | – | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 6 | – | 1 | 2 | 5 | 4 | – | 2 | 2 | 0 | 0 | – | – | – | 16 | 13 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 25 | 34 | – | 51 | 39 | 39 | 38 | – | 29 | 33 | 31 | 17 | – | – | – | 14 | 22 | 23 | 4 | 5 | 1 | 0 | 0 | 1 |
Endoplasmic Reticulum | 12 | 7 | – | 6 | 4 | 6 | 4 | – | 4 | 3 | 4 | 2 | – | – | – | 13 | 7 | 8 | 1 | 1 | 0 | 0 | 0 | 1 |
Endosome | 9 | 17 | – | 15 | 30 | 30 | 20 | – | 6 | 6 | 1 | 1 | – | – | – | 37 | 29 | 45 | 29 | 30 | 9 | 0 | 2 | 2 |
Golgi | 1 | 0 | – | 4 | 15 | 3 | 2 | – | 0 | 1 | 0 | 0 | – | – | – | 13 | 16 | 15 | 8 | 2 | 2 | 1 | 2 | 5 |
Mitochondria | 145 | 45 | – | 66 | 75 | 160 | 192 | – | 204 | 221 | 225 | 218 | – | – | – | 34 | 29 | 35 | 158 | 139 | 49 | 3 | 15 | 24 |
Nucleus | 0 | 2 | – | 3 | 5 | 10 | 16 | – | 6 | 11 | 8 | 10 | – | – | – | 5 | 3 | 8 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 2 | 0 | – | 0 | 0 | 0 | 1 | – | 1 | 1 | 3 | 2 | – | – | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 1 | 0 | 1 | – | 1 | 2 | 2 | 2 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 3 | 2 | – | 4 | 6 | 9 | 9 | – | 5 | 2 | 1 | 0 | – | – | – | 11 | 23 | 17 | 11 | 1 | 3 | 0 | 0 | 0 |
SpindlePole | 7 | 9 | – | 12 | 20 | 22 | 31 | – | 30 | 22 | 6 | 17 | – | – | – | 21 | 11 | 30 | 4 | 2 | 2 | 0 | 0 | 0 |
Vac/Vac Membrane | 17 | 24 | – | 15 | 32 | 30 | 18 | – | 11 | 26 | 11 | 8 | – | – | – | 27 | 24 | 50 | 4 | 12 | 6 | 0 | 2 | 3 |
Unique Cell Count | 192 | 120 | 154 | 189 | 262 | 285 | 255 | 280 | 256 | 253 | 166 | 160 | 199 | 235 | 202 | 84 | 8 | 26 | 45 | |||||
Labelled Cell Count | 224 | 151 | 188 | 238 | 320 | 352 | 312 | 345 | 304 | 288 | 205 | 193 | 256 | 235 | 202 | 84 | 8 | 26 | 45 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 5.0 | – | 5.8 | 6.5 | 5.1 | 5.0 | – | 4.1 | 4.5 | 4.0 | 4.1 | – | – | – | 6.5 | 6.7 | 6.2 | – | – | – |
Std Deviation (1e-4) | 0.8 | 0.8 | – | 2.2 | 2.3 | 1.8 | 1.7 | – | 1.3 | 1.3 | 1.6 | 0.8 | – | – | – | 1.4 | 1.7 | 1.3 | – | – | – |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.204 | 8.0442 | 7.177 | 8.5949 | 6.4012 | 7.5087 | 5.8334 | 7.7052 | 7.3511 | 6.3142 | 5.2053 | 7.927 | 6.4275 | 7.9165 | 6.9379 | 5.799 | 7.6332 | 7.43 |
Actin | 0.0168 | 0.0025 | 0.0011 | 0.0005 | 0.0011 | 0.0026 | 0.0202 | 0.0031 | 0.0132 | 0.0143 | 0.0095 | 0.0026 | 0.04 | 0.003 | 0.015 | 0.0072 | 0.0008 | 0.0037 |
Bud | 0.0002 | 0.0004 | 0.0002 | 0 | 0.0001 | 0 | 0.0007 | 0.0006 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0015 | 0.0003 | 0.0002 | 0 | 0 |
Bud Neck | 0.0007 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0014 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0003 | 0.0012 | 0.0003 | 0.0003 | 0.0003 | 0 | 0.0001 |
Bud Periphery | 0.0005 | 0.0006 | 0.0013 | 0.0001 | 0.0003 | 0.0001 | 0.0017 | 0.0017 | 0.0023 | 0.0004 | 0.0007 | 0.0001 | 0.0011 | 0.0024 | 0.0005 | 0.0004 | 0.0001 | 0.0001 |
Bud Site | 0.0009 | 0.0012 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0046 | 0.001 | 0.0025 | 0.0002 | 0.0008 | 0.0001 | 0.0022 | 0.0034 | 0.001 | 0.0005 | 0 | 0 |
Cell Periphery | 0.0004 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0007 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.0008 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
Cytoplasm | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0053 | 0 | 0.0007 | 0.0011 | 0.0003 | 0 | 0.0043 | 0.0002 | 0.001 | 0.0043 | 0 | 0.0003 |
Cytoplasmic Foci | 0.004 | 0.0037 | 0.0026 | 0.0049 | 0.007 | 0.0014 | 0.0305 | 0.0012 | 0.0075 | 0.0106 | 0.0183 | 0.003 | 0.0072 | 0.0026 | 0.0221 | 0.0152 | 0.0002 | 0.0006 |
Eisosomes | 0.0035 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0003 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0015 | 0.0002 | 0.0008 | 0.0005 | 0.0003 | 0 | 0.0021 | 0.0001 | 0.0004 | 0.0047 | 0 | 0.0008 |
Endosome | 0.0184 | 0.0127 | 0.0081 | 0.0199 | 0.0135 | 0.0055 | 0.0245 | 0.0074 | 0.0127 | 0.0291 | 0.0335 | 0.0034 | 0.014 | 0.0146 | 0.0146 | 0.0106 | 0.0014 | 0.0046 |
Golgi | 0.0151 | 0.0221 | 0.0253 | 0.0262 | 0.0244 | 0.0164 | 0.0136 | 0.0069 | 0.0125 | 0.0328 | 0.0331 | 0.0076 | 0.0112 | 0.0175 | 0.0136 | 0.0119 | 0.0027 | 0.0065 |
Lipid Particles | 0.0156 | 0.0045 | 0.0051 | 0.0188 | 0.0443 | 0.0033 | 0.0362 | 0.0101 | 0.01 | 0.036 | 0.0466 | 0.0023 | 0.021 | 0.0185 | 0.0163 | 0.0764 | 0.0007 | 0.0037 |
Mitochondria | 0.8991 | 0.9227 | 0.9356 | 0.9238 | 0.8781 | 0.9637 | 0.7534 | 0.9518 | 0.9028 | 0.8233 | 0.8005 | 0.97 | 0.8431 | 0.8899 | 0.8625 | 0.8227 | 0.9878 | 0.9438 |
None | 0.0011 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0245 | 0.0001 | 0.0006 | 0.004 | 0.0005 | 0 | 0.005 | 0 | 0.0016 | 0.0061 | 0 | 0.0013 |
Nuclear Periphery | 0.0044 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0009 | 0.0059 | 0.0002 | 0.0007 | 0.002 | 0.0008 | 0 | 0.007 | 0.0001 | 0.0006 | 0.0023 | 0 | 0.0143 |
Nucleolus | 0.0015 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0088 | 0.0002 | 0.0011 | 0.0002 | 0.0007 | 0 | 0.0013 | 0.0061 | 0.0002 | 0.0007 | 0 | 0.0004 |
Nucleus | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0016 | 0.0001 | 0.0006 | 0.0003 | 0.0004 | 0 | 0.0024 | 0.0008 | 0.0002 | 0.0008 | 0 | 0.0008 |
Peroxisomes | 0.0062 | 0.0071 | 0.0066 | 0.0033 | 0.0211 | 0.0029 | 0.0262 | 0.0095 | 0.0103 | 0.0207 | 0.0287 | 0.0078 | 0.0143 | 0.0051 | 0.0239 | 0.0224 | 0.0006 | 0.0016 |
Punctate Nuclear | 0.0009 | 0 | 0.0001 | 0 | 0.0011 | 0.0006 | 0.0095 | 0.0001 | 0.0032 | 0.0051 | 0.0016 | 0 | 0.0019 | 0 | 0.0016 | 0.0021 | 0 | 0.013 |
Vacuole | 0.0027 | 0.008 | 0.0016 | 0.0006 | 0.0039 | 0.0003 | 0.0079 | 0.0013 | 0.0092 | 0.0023 | 0.0061 | 0.0006 | 0.0071 | 0.0159 | 0.0078 | 0.0032 | 0.0006 | 0.0005 |
Vacuole Periphery | 0.005 | 0.0127 | 0.0111 | 0.0014 | 0.0036 | 0.0015 | 0.0204 | 0.0043 | 0.0069 | 0.0163 | 0.0163 | 0.0017 | 0.0121 | 0.0169 | 0.0162 | 0.0074 | 0.005 | 0.0039 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 32.8394 | 34.6686 | 24.8957 | 28.1827 | 25.5466 | 49.3881 | 36.4668 | 42.1658 | 41.2318 | 30.4287 |
Translational Efficiency | 1.016 | 0.7107 | 0.9571 | 1.0905 | 0.81 | 0.6666 | 0.6464 | 0.6763 | 0.7781 | 0.7392 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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