Standard name | |
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Human Ortholog | |
Description | RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication; Air2p and Air1p have nonredundant roles in regulation of substrate specificity of the exosome |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 1 | 1 | 4 | 1 | 4 | 3 | 5 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 3 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 5 | 19 | 9 | 13 | 11 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 7 | 0 | 9 | 16 | 6 | 14 | 24 | 16 | 19 | 11 | 8 | 7 | 19 | 37 | 4 | 7 | 14 | 0 | 0 | 0 | 3 | 0 | 3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 4 | 3 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 31 | 8 | 2 | 9 | 4 | 62 | 110 | 96 | 213 | 175 | 213 | 144 | 3 | 2 | 1 | 3 | 4 | 6 | 5 | 0 | 0 | 0 | 4 | 8 |
Nucleus | 294 | 105 | 55 | 153 | 284 | 230 | 305 | 304 | 323 | 434 | 325 | 336 | 205 | 220 | 198 | 100 | 88 | 99 | 281 | 102 | 77 | 147 | 178 | 167 |
Nuclear Periphery | 4 | 1 | 0 | 0 | 3 | 2 | 3 | 6 | 3 | 4 | 7 | 3 | 2 | 2 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6 |
Nucleolus | 2 | 2 | 0 | 3 | 0 | 1 | 5 | 10 | 13 | 12 | 26 | 21 | 5 | 1 | 4 | 4 | 6 | 2 | 4 | 0 | 0 | 11 | 22 | 35 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 6 | 2 | 1 | 1 | 4 | 0 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 6 | 5 |
Vac/Vac Membrane | 2 | 2 | 0 | 0 | 1 | 3 | 2 | 2 | 1 | 7 | 4 | 6 | 2 | 1 | 5 | 2 | 6 | 8 | 6 | 3 | 5 | 18 | 59 | 76 |
Unique Cell Count | 310 | 118 | 57 | 164 | 303 | 253 | 342 | 364 | 412 | 515 | 386 | 403 | 215 | 243 | 245 | 107 | 103 | 126 | 314 | 114 | 87 | 192 | 286 | 321 |
Labelled Cell Count | 340 | 126 | 57 | 176 | 312 | 308 | 449 | 457 | 594 | 664 | 605 | 535 | 224 | 250 | 251 | 117 | 113 | 132 | 314 | 114 | 87 | 192 | 286 | 321 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 6.0 | 5.3 | 5.7 | 5.8 | 4.7 | 4.4 | 5.0 | 4.2 | 4.5 | 4.1 | 4.5 | 6.3 | 6.6 | 6.3 | 8.3 | 9.0 | 9.2 | 5.6 | 6.1 | 6.5 |
Std Deviation (1e-4) | 0.7 | 1.1 | 0.6 | 1.6 | 1.5 | 1.2 | 1.1 | 1.6 | 0.9 | 1.2 | 1.0 | 0.9 | 1.1 | 1.4 | 1.4 | 1.7 | 2.0 | 2.0 | 1.3 | 1.6 | 1.2 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.14 | -0.17 | -0.28 | -0.08 | -0.35 | -0.23 | -0.36 | -0.23 | 0.26 | 0.33 | 0.24 | 0.64 | 0.76 | 0.79 | 0.09 | 0.22 | 0.3 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.9 | -0.8 | -1.4 | -1.7 | -2.6 | -3.2 | -2.5 | -2.5 | -2.6 | -0.4 | -1.5 | -2.9 | -0.8 | -2.2 | -3.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7817 | 2.9134 | 2.6315 | 2.5495 | 2.9027 | 2.5433 | 2.0906 | 2.4186 | 2.1561 | 1.593 | 1.6601 | 1.9728 | 1.1219 | 1.1046 | 0.801 | 0.952 | 0.5929 | 0.7886 |
Actin | 0.0271 | 0.0001 | 0.0143 | 0.016 | 0.0116 | 0.0012 | 0.0109 | 0 | 0.0046 | 0 | 0.0045 | 0.0002 | 0.0636 | 0.0002 | 0.0057 | 0.016 | 0.0269 | 0.0017 |
Bud | 0.0005 | 0 | 0.0002 | 0.0017 | 0.0013 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0013 | 0.0003 | 0.0003 | 0.0083 | 0.0002 | 0.0002 |
Bud Neck | 0.0012 | 0.0002 | 0.0007 | 0.0009 | 0.001 | 0.0021 | 0.0011 | 0.0001 | 0.0007 | 0.0001 | 0.0032 | 0.0012 | 0.0039 | 0.0002 | 0.0005 | 0.0013 | 0.0008 | 0.0012 |
Bud Periphery | 0.0006 | 0.0001 | 0.0004 | 0.0025 | 0.0073 | 0.0007 | 0.0002 | 0 | 0.0002 | 0 | 0.0008 | 0.0003 | 0.0025 | 0.0005 | 0.0005 | 0.0401 | 0.0003 | 0.0005 |
Bud Site | 0.0013 | 0.0002 | 0.001 | 0.0117 | 0.0009 | 0.0003 | 0.0006 | 0 | 0.0003 | 0 | 0.0009 | 0.0001 | 0.0124 | 0.0004 | 0.0124 | 0.0065 | 0.0009 | 0.0002 |
Cell Periphery | 0.0003 | 0 | 0.0002 | 0.0004 | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0.0018 | 0.0001 | 0.0001 |
Cytoplasm | 0.0096 | 0.0051 | 0.0072 | 0.0021 | 0.002 | 0.0008 | 0.0023 | 0.0001 | 0.0007 | 0.0001 | 0.0015 | 0.0001 | 0.001 | 0.001 | 0.0008 | 0.0016 | 0.0661 | 0.0001 |
Cytoplasmic Foci | 0.0129 | 0.0004 | 0.0054 | 0.0105 | 0.0095 | 0.001 | 0.009 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0046 | 0 | 0.0019 | 0.0063 | 0.0224 | 0.0001 |
Eisosomes | 0.0006 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0027 | 0.0003 | 0.0019 | 0.0029 | 0.0045 | 0.0006 | 0.0008 | 0 | 0.0004 | 0 | 0.0012 | 0.0001 | 0.0014 | 0.0001 | 0.0006 | 0.0016 | 0.0019 | 0.0003 |
Endosome | 0.0168 | 0.0004 | 0.0087 | 0.0357 | 0.0277 | 0.0066 | 0.0082 | 0 | 0.001 | 0 | 0.0141 | 0.001 | 0.017 | 0.0006 | 0.009 | 0.0187 | 0.0243 | 0.0022 |
Golgi | 0.0075 | 0 | 0.0039 | 0.0091 | 0.02 | 0.0016 | 0.0021 | 0 | 0.0006 | 0 | 0.0011 | 0.0001 | 0.0141 | 0.0002 | 0.0049 | 0.0152 | 0.006 | 0.0031 |
Lipid Particles | 0.0191 | 0 | 0.0125 | 0.0222 | 0.0918 | 0.0067 | 0.0069 | 0 | 0.0006 | 0 | 0.0023 | 0.0001 | 0.0076 | 0.0002 | 0.0012 | 0.0056 | 0.0277 | 0.0006 |
Mitochondria | 0.024 | 0.0005 | 0.0125 | 0.0736 | 0.0164 | 0.0108 | 0.0012 | 0.0002 | 0.0009 | 0.0001 | 0.0054 | 0.0013 | 0.0306 | 0.0106 | 0.0147 | 0.0138 | 0.0061 | 0.0224 |
None | 0.013 | 0.0017 | 0.0116 | 0.0021 | 0.0043 | 0.0006 | 0.0044 | 0 | 0.0003 | 0 | 0.0014 | 0.0003 | 0.001 | 0.0007 | 0.0002 | 0.0006 | 0.0032 | 0.0001 |
Nuclear Periphery | 0.0139 | 0.0055 | 0.0074 | 0.0157 | 0.0584 | 0.0072 | 0.0105 | 0.0065 | 0.006 | 0.0007 | 0.0406 | 0.0031 | 0.0079 | 0.0069 | 0.0051 | 0.012 | 0.0144 | 0.0016 |
Nucleolus | 0.0512 | 0.0728 | 0.122 | 0.0699 | 0.0267 | 0.1192 | 0.0527 | 0.0544 | 0.1005 | 0.0361 | 0.0372 | 0.1199 | 0.0347 | 0.039 | 0.091 | 0.1395 | 0.0205 | 0.1154 |
Nucleus | 0.7593 | 0.9071 | 0.7776 | 0.6937 | 0.6609 | 0.8318 | 0.8731 | 0.9348 | 0.8789 | 0.9301 | 0.8327 | 0.8695 | 0.7754 | 0.9353 | 0.8437 | 0.6897 | 0.7439 | 0.848 |
Peroxisomes | 0.0165 | 0 | 0.0058 | 0.0109 | 0.0265 | 0.0006 | 0.0033 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0097 | 0.0001 | 0.0014 | 0.0032 | 0.0173 | 0.0003 |
Punctate Nuclear | 0.0166 | 0.005 | 0.004 | 0.0036 | 0.0075 | 0.0045 | 0.0106 | 0.0033 | 0.0024 | 0.0327 | 0.0431 | 0.0015 | 0.0013 | 0.0008 | 0.0019 | 0.0013 | 0.0105 | 0.0006 |
Vacuole | 0.0033 | 0.0003 | 0.0015 | 0.0075 | 0.0102 | 0.0019 | 0.0009 | 0.0001 | 0.0008 | 0 | 0.0054 | 0.0006 | 0.0055 | 0.0016 | 0.0021 | 0.0127 | 0.0038 | 0.0005 |
Vacuole Periphery | 0.0019 | 0.0001 | 0.0011 | 0.0071 | 0.0109 | 0.0011 | 0.0006 | 0.0002 | 0.0004 | 0 | 0.0024 | 0.0005 | 0.0033 | 0.0013 | 0.0016 | 0.0039 | 0.0024 | 0.0008 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.9957 | 7.6242 | 16.2652 | 10.6315 | 11.0579 | 9.5895 | 15.8994 | 10.4058 | 11.0332 | 13.7729 |
Translational Efficiency | 2.6283 | 2.5856 | 0.9898 | 1.1932 | 1.6585 | 2.7033 | 1.4672 | 1.8302 | 1.6504 | 1.4297 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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