Standard name
Human Ortholog
Description Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase; inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has a calmodulin-binding motif and binds calmodulin in vitro; INH1 has a paralog, STF1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0.16 0.14 0 0 0 0 0 0.06
Bud 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.11 0.16 0.08 0 0 0 0 0 0
Cytoplasm 0.72 0.14 0.3 0.2 0.07 0 – 0 0 0 0 0 0.4 0.34 0.33 0.26 0.34 0.18 0.41 0 0.05 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Endosome 0 0.07 0 0 0.26 0 – 0 0 0 0 0 0.3 0.14 0.23 0.13 0.09 0.12 0 0.05 0.1 0.09 0.1 0.08
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.28 0.28 0.26 0 0.08 0 0 0.05 0.05
Mitochondria 0.28 0.69 0.74 0.86 0.73 0.88 – 0.9 0.92 0.92 0.95 0.91 0.21 0.41 0.29 0.07 0.18 0.08 0.35 0.66 0.66 0.66 0.56 0.53
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.05 – 0.07 0.07 0.06 0.07 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.12 – 0.19 0.12 0.12 0.1 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0.08 0 0.12 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.12 0 0.05 0.14 0.08 – 0.06 0.05 0 0 0 0.13 0.08 0.13 0.23 0.11 0.26 0.06 0.05 0 0.06 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 3 1 0 0 – 0 0 0 0 1 0 1 2 8 12 9 6 9 7 4 7 13
Bud 1 4 13 8 5 5 – 18 7 12 11 15 3 5 4 3 3 0 3 1 0 1 3 7
Bud Neck 1 0 6 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 1 2 1 5 5
Bud Site 0 1 1 1 0 0 – 7 2 4 1 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 6 0 3 1 3 – 4 0 2 3 3 1 6 8 10 12 5 1 0 1 1 0 1
Cytoplasm 147 25 74 39 17 12 – 4 4 2 1 3 58 84 79 23 25 12 102 6 15 1 7 9
Endoplasmic Reticulum 0 7 4 2 0 4 – 1 2 0 2 4 0 4 8 6 0 3 1 1 3 1 0 3
Endosome 4 12 1 2 65 7 – 2 2 3 0 1 43 35 55 12 7 8 9 9 28 14 23 20
Golgi 0 3 0 0 1 0 – 1 0 0 1 0 5 6 7 25 21 17 1 16 1 6 11 11
Mitochondria 58 122 184 169 182 287 – 378 283 317 276 322 30 101 71 6 13 5 85 137 191 106 126 126
Nucleus 1 2 3 0 1 4 – 2 1 1 0 2 0 2 1 1 0 1 1 1 0 0 0 1
Nuclear Periphery 0 0 1 0 4 16 – 28 22 19 20 32 0 0 0 0 0 0 0 0 1 1 0 2
Nucleolus 0 3 0 2 6 38 – 79 38 43 28 36 0 0 0 1 0 2 0 0 0 0 1 2
Peroxisomes 1 8 1 1 8 2 – 2 1 8 3 8 11 11 30 0 0 0 1 6 7 5 9 5
SpindlePole 1 1 7 1 7 2 – 3 2 5 3 4 5 2 2 3 2 1 6 1 10 3 7 9
Vac/Vac Membrane 20 22 6 10 35 25 – 27 15 10 10 8 19 20 32 21 8 17 14 10 6 10 16 13
Unique Cell Count 205 178 248 197 251 325 421 308 344 292 353 144 245 242 90 74 66 247 208 288 163 225 238
Labelled Cell Count 235 219 304 240 332 405 556 379 426 359 444 175 277 299 119 103 80 247 208 288 163 225 238


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 7.2 3.5 4.1 5.7 5.8 – 7.7 9.1 10.1 10.7 11.8 5.7 6.0 5.6 9.9 9.3 9.6 6.3 6.1 6.5
Std Deviation (1e-4) 2.1 2.6 0.8 1.8 1.5 1.7 – 3.0 4.2 4.9 5.3 5.7 2.5 2.6 1.3 3.6 2.2 2.4 5.4 3.1 4.4
Intensity Change (Log2) – – – 0.25 0.72 0.73 – 1.15 1.39 1.54 1.63 1.77 0.71 0.79 0.69 1.51 1.42 1.47 0.86 0.81 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -1.9 -2.5 – -0.6 -1.9 -1.0 -0.8 -0.6 -1.5 -1.9 -2.2 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.4 -6.7 -8.7 – -11.2 -9.6 -10.5 -9.9 -10.5 2.1 1.1 0.7 -0.8 0.6 -1.9
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 1.2 0 0 0
Endosome 0 8.4 0 – 0 0 0 0 0 8.9 5.9 7.8 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 8.6 0 0
Mitochondria 0 -0.4 4.4 – 5.3 5.7 6.0 6.6 5.6 -10.2 -7.4 -9.9 -11.1 -8.8 -9.8
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 3.2 – 3.8 4.0 3.4 3.9 4.6 0 0 0 0 0 0
Nucleolus 0 0 5.6 – 7.3 5.7 5.8 5.0 5.2 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 2.9 5.5 0 0 0
SpindlePole 0 0 0 – 0 0 -1.2 0 0 0 0 0 0 0 0
Vacuole 1.5 4.7 2.8 – 2.3 1.5 0.4 0.7 -0.1 4.2 2.9 4.5 6.3 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.9508 4.1066 4.365 3.298 3.5295 4.2107 3.7222 3.0879 3.0698 3.1288 2.9592 3.1651 10.9378 6.3585 7.0636 3.9449 5.4566 9.1735
Actin 0.0069 0.0169 0.0053 0.004 0.0035 0.002 0.0238 0.0314 0.038 0.02 0.0339 0.0075 0.0644 0.0252 0.0487 0.0442 0.0052 0.0218
Bud 0.0004 0.0017 0.0013 0.0003 0.0003 0.0002 0.0008 0.0028 0.0021 0.0007 0.002 0.0003 0.0035 0.005 0.006 0.0278 0.0013 0.0009
Bud Neck 0.0002 0.0004 0.0004 0.0001 0.0001 0.0002 0.0009 0.0005 0.0044 0.0007 0.0004 0.0004 0.0011 0.0007 0.0009 0.0008 0.0003 0.0016
Bud Periphery 0.0008 0.003 0.0018 0.0006 0.0006 0.0004 0.0014 0.0037 0.0057 0.001 0.0026 0.0007 0.0044 0.0097 0.0142 0.0412 0.0047 0.0024
Bud Site 0.0013 0.0105 0.0082 0.0002 0.0003 0.0002 0.0025 0.011 0.0087 0.0013 0.0031 0.0003 0.0046 0.0247 0.0057 0.0153 0.001 0.0017
Cell Periphery 0.0002 0.0003 0.003 0.0002 0.0001 0.0001 0.0004 0.0005 0.0008 0.0003 0.0002 0.0002 0.0005 0.0013 0.0019 0.0019 0.0009 0.001
Cytoplasm 0.0027 0.0017 0.001 0.0007 0.0004 0.0029 0.0036 0.0037 0.0037 0.0198 0.0029 0.0014 0.0695 0.0346 0.0523 0.0299 0.0106 0.0566
Cytoplasmic Foci 0.0174 0.0389 0.0145 0.0137 0.0171 0.012 0.0195 0.0335 0.0149 0.0264 0.0296 0.0135 0.0444 0.0442 0.0353 0.0222 0.0343 0.0383
Eisosomes 0.0002 0.0002 0.0004 0.0004 0.0002 0.0001 0.0007 0.0005 0.0005 0.0003 0.0003 0.0002 0.0005 0.0009 0.0004 0.0003 0.0022 0.0013
Endoplasmic Reticulum 0.0007 0.0003 0.0003 0.0005 0.0003 0.0005 0.0023 0.0005 0.0009 0.0012 0.0062 0.0004 0.0034 0.0007 0.0021 0.0203 0.0014 0.0014
Endosome 0.0335 0.0314 0.0284 0.0542 0.0442 0.0241 0.059 0.0736 0.034 0.0749 0.0648 0.0396 0.1027 0.0819 0.0773 0.1011 0.0885 0.0913
Golgi 0.0443 0.0761 0.0312 0.0371 0.0608 0.0409 0.0679 0.0875 0.0542 0.1314 0.0751 0.0585 0.1033 0.0901 0.0595 0.1167 0.1213 0.1071
Lipid Particles 0.0192 0.0092 0.005 0.0017 0.0017 0.0063 0.0199 0.0056 0.0059 0.0057 0.0218 0.0122 0.064 0.0185 0.0085 0.0101 0.0073 0.0193
Mitochondria 0.8424 0.7632 0.8688 0.8682 0.847 0.8928 0.7575 0.7164 0.7796 0.6855 0.6821 0.8422 0.4485 0.5985 0.6193 0.5273 0.5791 0.6127
None 0.0028 0.0009 0.0003 0.0001 0.0001 0.0004 0.002 0.0004 0.0014 0.0038 0.0008 0.0002 0.0052 0.0128 0.0054 0.0032 0.1055 0.0025
Nuclear Periphery 0.0005 0.0001 0.0003 0.0008 0.0002 0.0003 0.0022 0.0002 0.0056 0.0004 0.0228 0.0001 0.0146 0.0002 0.0009 0.0033 0.0005 0.0008
Nucleolus 0.0006 0.0007 0.0008 0.0001 0 0.0001 0.0011 0.0005 0.0006 0.0003 0.0002 0.0001 0.0005 0.0019 0.0016 0.0005 0.0005 0.0003
Nucleus 0.0003 0.0003 0.0003 0.0001 0.0001 0.0001 0.0009 0.0002 0.0033 0.0009 0.0024 0.0001 0.0027 0.0009 0.0016 0.0011 0.0005 0.0008
Peroxisomes 0.0102 0.0374 0.015 0.0027 0.0136 0.0117 0.0227 0.0207 0.0242 0.0099 0.0373 0.0173 0.0474 0.0395 0.0347 0.0071 0.0242 0.0288
Punctate Nuclear 0.0005 0.0002 0.0002 0 0.0001 0.0002 0.002 0.0001 0.0051 0.0003 0.0044 0.0002 0.0061 0.0003 0.0004 0.0008 0.0006 0.001
Vacuole 0.0049 0.004 0.0064 0.0044 0.0036 0.0011 0.005 0.0037 0.0041 0.0105 0.0034 0.0018 0.0044 0.005 0.0163 0.0151 0.0045 0.004
Vacuole Periphery 0.0099 0.0027 0.007 0.01 0.0056 0.0034 0.0038 0.003 0.0023 0.0048 0.0038 0.0029 0.0041 0.0035 0.0071 0.0099 0.0054 0.0043

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.6606 3.8232 2.837 16.3297 19.4075 17.0976 10.6304 14.3496 12.9379 12.9603
Translational Efficiency 1.7666 6.7196 8.8223 2.2143 2.6431 2.461 1.7361 1.7875 2.552 1.83

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase; inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has a calmodulin-binding motif and binds calmodulin in vitro; INH1 has a paralog, STF1, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (76%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Inh1

Inh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Inh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available