Standard name | |
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Human Ortholog | |
Description | Homocitrate synthase isozyme and functions in DNA repair; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 4 | 0 | 3 | 2 | 4 | 4 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 70 | 12 | 41 | 14 | 27 | 30 | 33 | 45 | 44 | 18 | 13 | 13 | 22 | 22 | 11 | 34 | 21 | – | 3 | 0 | 1 | 1 | 3 | 3 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 7 | – | 0 | 0 | 0 | 1 | 1 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 42 | 12 | 76 | 50 | 83 | 260 | 359 | 249 | 223 | 311 | 225 | 260 | 27 | 14 | 25 | 7 | 6 | – | 0 | 0 | 1 | 1 | 2 | 2 |
Nucleus | 232 | 96 | 328 | 242 | 259 | 401 | 536 | 433 | 380 | 533 | 389 | 458 | 361 | 366 | 336 | 66 | 63 | – | 221 | 91 | 295 | 292 | 303 | 268 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 7 | 5 | 15 | 9 | 10 | 10 |
Nucleolus | 13 | 18 | 53 | 56 | 97 | 274 | 369 | 343 | 296 | 432 | 309 | 388 | 50 | 50 | 61 | 9 | 5 | – | 9 | 3 | 17 | 20 | 30 | 40 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 7 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 1 | 6 | 3 | 3 | 0 | 2 | 13 | 2 | 2 | 1 | 3 | 1 | 6 | 10 | 1 | 1 | – | 3 | 1 | 7 | 11 | 25 | 30 |
Unique Cell Count | 261 | 112 | 371 | 269 | 329 | 560 | 774 | 690 | 588 | 778 | 568 | 695 | 396 | 415 | 404 | 92 | 81 | 267 | 106 | 362 | 359 | 406 | 385 | |
Labelled Cell Count | 360 | 140 | 506 | 365 | 469 | 965 | 1300 | 1084 | 947 | 1299 | 939 | 1123 | 464 | 463 | 454 | 120 | 103 | 267 | 106 | 362 | 359 | 406 | 385 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 172.8 | 182.3 | 151.4 | 135.4 | 106.0 | 86.6 | 73.9 | 70.9 | 61.9 | 53.2 | 47.6 | 44.1 | 155.9 | 153.8 | 140.1 | 240.2 | 218.5 | – | 143.2 | 151.0 | 152.6 |
Std Deviation (1e-4) | 57.8 | 75.3 | 48.5 | 42.6 | 37.4 | 33.5 | 31.2 | 32.0 | 26.9 | 20.4 | 20.0 | 18.4 | 49.4 | 52.8 | 48.9 | 86.6 | 85.1 | – | 50.1 | 53.1 | 58.3 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.51 | -0.81 | -1.04 | -1.09 | -1.29 | -1.51 | -1.67 | -1.78 | 0.04 | 0.02 | -0.11 | 0.67 | 0.53 | – | -0.08 | -0.0 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Cytoplasm | -2.6 | -1.3 | -3.2 | -4.4 | -2.6 | -1.9 | -6.3 | -5.6 | -6.5 | -2.8 | -3.0 | -4.6 | 6.0 | 3.5 | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Mitochondria | -0.6 | 1.5 | 8.1 | 8.4 | 5.3 | 5.7 | 6.5 | 6.1 | 5.7 | -5.5 | -7.5 | -5.9 | -2.9 | -2.8 | – |
Nucleus | 0.6 | -3.5 | -6.1 | -7.1 | -8.8 | -8.2 | -7.3 | -7.0 | -8.0 | 1.3 | -0.1 | -2.1 | -4.0 | -2.5 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Nucleolus | 2.2 | 4.9 | 10.8 | 11.0 | 11.4 | 11.3 | 13.2 | 12.3 | 13.1 | -0.7 | -0.9 | 0.3 | -1.1 | -2.0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – |
Vacuole | 0 | 0 | 0 | 0 | 0.3 | 0 | 0 | 0 | 0 | 0 | -0.2 | 0.8 | 0 | 0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 488.6892 | 451.4349 | 437.024 | 425.9124 | 417.7193 | 425.1257 | 457.8695 | 526.9491 | 506.2605 | 507.4828 | 465.8065 | 462.6642 | 566.2144 | 562.1984 | 520.4659 | 536.2219 | 461.3776 | 531.7499 |
Actin | 0 | 0 | 0 | 0 | 0 | 0 | 0.0294 | 0 | 0.002 | 0 | 0 | 0.0103 | 0.0067 | 0 | 0.0012 | 0 | 0.0034 | 0 |
Bud | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0003 | 0 | 0 | 0.0012 | 0.0007 | 0 | 0.0014 | 0 | 0.0011 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0.0015 | 0 | 0.0004 | 0 | 0 | 0.0006 | 0.0006 | 0 | 0.0002 | 0 | 0.0007 | 0 |
Bud Periphery | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0013 | 0 | 0.0006 | 0 | 0 | 0.0062 | 0.0008 | 0 | 0.0053 | 0 | 0.0092 | 0 |
Bud Site | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0031 | 0 | 0.0008 | 0 | 0 | 0.0006 | 0.0027 | 0 | 0.0009 | 0 | 0.0009 | 0 |
Cell Periphery | 0.001 | 0 | 0 | 0 | 0.0001 | 0.0003 | 0.0022 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0023 | 0.0018 | 0.0001 | 0.0006 | 0.0001 | 0.0038 | 0.0001 |
Cytoplasm | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0087 | 0 | 0.0002 | 0 | 0 | 0.0016 | 0.0074 | 0 | 0.0003 | 0 | 0.0003 | 0 |
Cytoplasmic Foci | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0162 | 0 | 0.0026 | 0 | 0 | 0.0009 | 0.0015 | 0 | 0.0024 | 0 | 0.0032 | 0 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0022 | 0 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0.0053 | 0 | 0.0001 | 0 | 0 | 0.0014 | 0.0025 | 0 | 0.0009 | 0 | 0.0004 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0.015 | 0 | 0.0029 | 0 | 0 | 0.0125 | 0.0072 | 0 | 0.001 | 0 | 0.0019 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0.0029 | 0 | 0.0011 | 0 | 0 | 0.0031 | 0.0013 | 0 | 0.0007 | 0 | 0.001 | 0 |
Lipid Particles | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0049 | 0 | 0.0036 | 0 | 0 | 0.0013 | 0.0014 | 0 | 0.0003 | 0 | 0.0012 | 0 |
Mitochondria | 0.0045 | 0.0012 | 0.0038 | 0.0041 | 0.0089 | 0.0018 | 0.0172 | 0.003 | 0.0103 | 0.0016 | 0.0172 | 0.0159 | 0.0058 | 0.0042 | 0.0133 | 0.0038 | 0.0187 | 0.0015 |
None | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0078 | 0 | 0.0004 | 0 | 0 | 0.0047 | 0.0014 | 0 | 0.0012 | 0 | 0.0002 | 0 |
Nuclear Periphery | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0154 | 0.0026 | 0.0006 | 0.0001 | 0 | 0.0137 | 0.0176 | 0.0012 | 0.0011 | 0.0005 | 0.0006 | 0 |
Nucleolus | 0.0746 | 0.0333 | 0.0458 | 0.058 | 0.1894 | 0.0836 | 0.1011 | 0.0396 | 0.0466 | 0.03 | 0.294 | 0.0699 | 0.0684 | 0.044 | 0.0559 | 0.0884 | 0.2691 | 0.0789 |
Nucleus | 0.917 | 0.9653 | 0.9499 | 0.9374 | 0.8009 | 0.9136 | 0.7429 | 0.9513 | 0.9121 | 0.9682 | 0.6876 | 0.8469 | 0.8539 | 0.9489 | 0.904 | 0.9048 | 0.6749 | 0.9189 |
Peroxisomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0087 | 0 | 0.0098 | 0 | 0 | 0.0006 | 0.0007 | 0 | 0.0002 | 0 | 0.0005 | 0 |
Punctate Nuclear | 0 | 0 | 0 | 0 | 0 | 0 | 0.0067 | 0 | 0.0006 | 0 | 0 | 0.0019 | 0.0131 | 0 | 0.0059 | 0 | 0.0001 | 0 |
Vacuole | 0.0009 | 0 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0043 | 0.0004 | 0.0012 | 0 | 0.0001 | 0.002 | 0.0028 | 0.0002 | 0.0015 | 0.0003 | 0.0062 | 0.0001 |
Vacuole Periphery | 0.0007 | 0.0001 | 0.0003 | 0.0003 | 0.0005 | 0.0003 | 0.0025 | 0.003 | 0.0037 | 0.0001 | 0.0008 | 0.0022 | 0.0016 | 0.0013 | 0.0013 | 0.002 | 0.0025 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1129.9822 | 1189.4762 | 1166.4304 | 1080.6605 | 893.1599 | 1422.4067 | 1485.7182 | 1291.5973 | 1301.1755 | 1140.4771 |
Translational Efficiency | 1.0265 | 1.0302 | 0.9834 | 1.0794 | 1.0491 | 1.0571 | 1.066 | 1.134 | 0.9857 | 1.0678 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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