Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0.06 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 1.0 0.98 0.92 – 0.78 0.83 0.78 0.78 0.74 0.99 0.99 0.99 0.99 0.99 0.98 0.98 0.92 0.96 0.92 0.95 0.96
Endoplasmic Reticulum 0 0 0 0 0 0.06 – 0.19 0.13 0.16 0.13 0.14 0 0 0 0.06 0.05 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 – 3 4 8 11 16 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 2 1 1 1 – 1 2 0 4 2 3 4 3 0 0 0 0 1 0 0 3 8
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 7 14 – 12 14 21 19 20 2 3 6 3 0 1 0 0 0 0 0 0
Cytoplasm 316 137 130 217 284 307 – 364 276 270 245 272 472 806 825 218 353 336 303 129 117 114 403 574
Endoplasmic Reticulum 0 0 0 0 2 20 – 90 44 54 42 52 2 5 9 13 18 26 0 1 0 0 1 2
Endosome 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 1 0 – 1 3 5 5 9 1 0 0 0 1 2 0 0 0 0 0 0
Mitochondria 0 0 0 0 1 0 – 1 5 3 11 21 0 0 0 0 2 6 0 0 1 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 1 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 1 0 0 0 3 1 0 1 2 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 0 0 0 – 1 0 0 0 0 0 1 0 1 2 1 2 2 2 4 7 5
Unique Cell Count 318 138 130 217 290 333 466 334 345 314 368 475 814 834 221 356 342 311 141 122 125 425 601
Labelled Cell Count 318 138 132 219 296 343 474 348 362 337 395 481 819 844 237 376 372 311 141 122 125 425 601


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 196.0 241.8 108.9 156.9 128.2 98.6 – 73.4 64.5 59.2 54.3 49.8 205.8 207.6 186.2 262.7 240.1 250.3 187.4 211.2 225.8
Std Deviation (1e-4) 42.1 52.4 25.5 32.3 32.1 22.7 – 17.9 15.9 13.0 12.7 12.5 50.7 46.1 43.1 86.9 91.2 82.2 47.1 59.3 63.9
Intensity Change (Log2) – – – 0.53 0.24 -0.14 – -0.57 -0.75 -0.88 -1.0 -1.13 0.92 0.93 0.77 1.27 1.14 1.2 0.78 0.96 1.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 2.9 2.9 2.7 0 0 0 0 0 0
Cytoplasm 0 -1.7 -3.3 – -5.9 -5.1 -5.8 -5.8 -6.5 -0.9 -1.1 -1.2 -1.3 -1.0 -1.5
Endoplasmic Reticulum 0 0 2.9 – 5.4 4.3 4.8 4.4 4.5 0 0 0 0 0 3.2
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 2.8 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 160.8752 285.6369 222.2298 172.1388 211.4679 233.9378 192.9565 277.8344 233.928 212.5582 229.0459 190.0723 210.1999 285.7337 289.8093 257.5035 280.1833 211.3374
Actin 0.0103 0.0004 0.002 0.0014 0.0054 0.0077 0.022 0.0003 0.0018 0.0077 0.0017 0.0021 0.0301 0.0023 0.0008 0.0013 0.0006 0.0034
Bud 0.0076 0.001 0.0025 0.0041 0.0077 0.0048 0.0037 0.0007 0.0036 0.003 0.0026 0.0011 0.0042 0.002 0.0033 0.007 0.0014 0.0026
Bud Neck 0.0027 0.0009 0.0014 0.0014 0.002 0.0045 0.0016 0.0009 0.0013 0.0018 0.0014 0.0032 0.0014 0.0006 0.0014 0.0014 0.0006 0.0011
Bud Periphery 0.0078 0.0006 0.0019 0.0052 0.0063 0.0069 0.0057 0.0004 0.0043 0.0013 0.0013 0.0019 0.0033 0.0007 0.0056 0.0051 0.0002 0.0007
Bud Site 0.0047 0.0013 0.0023 0.0023 0.0055 0.0012 0.0045 0.0011 0.0025 0.0015 0.0008 0.0009 0.0083 0.0108 0.0009 0.0016 0.0001 0.0004
Cell Periphery 0.0735 0.0574 0.0545 0.0537 0.0332 0.02 0.0806 0.0469 0.0347 0.0558 0.0107 0.0411 0.0291 0.0321 0.0197 0.0352 0.0016 0.0029
Cytoplasm 0.4047 0.7947 0.5962 0.3124 0.5705 0.6266 0.4856 0.8529 0.7733 0.3928 0.7181 0.5178 0.5453 0.8545 0.8254 0.5401 0.9115 0.5954
Cytoplasmic Foci 0.0291 0.0005 0.0224 0.0364 0.021 0.0117 0.0141 0.0005 0.0026 0.0034 0.0205 0.0065 0.0174 0.0021 0.0012 0.0048 0.0015 0.0415
Eisosomes 0.0017 0 0.0007 0.0022 0.0025 0.0001 0.0012 0.0001 0.001 0 0.0012 0.0004 0.0027 0.0001 0.0001 0.0004 0 0.0005
Endoplasmic Reticulum 0.0316 0.0041 0.0097 0.0057 0.0038 0.0175 0.0171 0.0028 0.0024 0.0027 0.0054 0.042 0.0169 0.0012 0.0018 0.0016 0.0003 0.004
Endosome 0.0185 0.0007 0.0097 0.0557 0.0127 0.0306 0.008 0.0004 0.0016 0.004 0.0056 0.0053 0.0125 0.0007 0.0014 0.0026 0.0008 0.0433
Golgi 0.0029 0.0001 0.0028 0.0038 0.0019 0.0053 0.0026 0.0001 0.0003 0.0004 0.0009 0.001 0.0034 0.0003 0.0002 0.0003 0.0001 0.0044
Lipid Particles 0.0102 0.0014 0.0054 0.0117 0.013 0.0085 0.0068 0.0011 0.0035 0.0038 0.0057 0.004 0.0081 0.0012 0.0007 0.0016 0.0003 0.0352
Mitochondria 0.0248 0.0009 0.0172 0.0203 0.0198 0.0149 0.0207 0.0009 0.0115 0.0011 0.0126 0.0168 0.0291 0.0029 0.0128 0.0193 0.0021 0.0161
None 0.0219 0.0006 0.013 0.0281 0.0081 0.0018 0.0109 0.001 0.0039 0.0007 0.0132 0.0284 0.0199 0.0015 0.0014 0.0181 0.0015 0.0113
Nuclear Periphery 0.0677 0.0102 0.0197 0.0224 0.0158 0.0339 0.1039 0.0101 0.0161 0.0408 0.0288 0.0743 0.064 0.0069 0.0109 0.016 0.0141 0.0598
Nucleolus 0.0321 0.002 0.0167 0.0382 0.0116 0.0031 0.0094 0.0025 0.0212 0.0068 0.0253 0.0463 0.0189 0.0029 0.0019 0.0116 0.0054 0.0891
Nucleus 0.0357 0.0102 0.0203 0.0223 0.0209 0.0307 0.0529 0.0125 0.0225 0.0281 0.0442 0.0967 0.0656 0.0122 0.0136 0.0393 0.0295 0.0458
Peroxisomes 0.0014 0.0001 0.0011 0.0015 0.0035 0.0037 0.0011 0.0001 0.0005 0.0003 0.001 0.0003 0.0014 0.0002 0.0002 0.0006 0.0001 0.002
Punctate Nuclear 0.0019 0.0001 0.0011 0.0013 0.0009 0.0036 0.0073 0.0001 0.0004 0.0004 0.0025 0.0044 0.0031 0.0001 0.0001 0.0002 0.0003 0.0109
Vacuole 0.1734 0.1047 0.1649 0.2587 0.1848 0.1094 0.1116 0.0596 0.0738 0.3227 0.0673 0.0768 0.0842 0.0572 0.0793 0.2096 0.0137 0.0152
Vacuole Periphery 0.0358 0.0081 0.0345 0.1114 0.0488 0.0534 0.0287 0.0049 0.0173 0.1208 0.0294 0.0285 0.0312 0.0076 0.0173 0.0821 0.0142 0.0144

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8275.5333 10205.1996 14995.2567 7777.3471 14333.6327 14619.0843 8934.3602 10247.9185 14689.8232 7075.598
Translational Efficiency 2.5456 2.2162 1.3227 3.7872 1.581 1.2385 1.9004 2.0393 1.2259 2.1895

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rpl41a

Rpl41a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl41a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available