Standard name
Human Ortholog
Description Ubiquitin chain assembly factor (E4); cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 0.9 0.74 0.82 0.68 0.68 0.59 0.54 0.48 – 0.48 0.4 0.68 0.69 0.55 0.72 0.6 0.69 0.72 0.62 0.77 0.66 0.54 0.44
Endoplasmic Reticulum 0.1 0 0.06 0.06 0.06 0.08 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0 0.09 0.06 0 0.12
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.06 0.11 0.12 0.25 – 0.33 0.35 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.05 0.05 – 0.05 0.05 0 0 0 0.05 0 0 0.05 0.15 0 0.13 0.15 0.14
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.15 0.26 0.13 0.36 0.25 0.36 0.37 0.34 – 0.22 0.38 0.32 0.41 0.53 0.37 0.52 0.41 0 0.09 0.05 0.08 0.12 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 2 0 0 0 0 1 0 0 1 0 1 0 2 1
Bud 0 0 1 1 1 1 2 1 4 – 1 5 0 0 1 0 0 0 1 1 1 0 8 8
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 1 0 2 3
Bud Site 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 1 4 5 5 6 – 11 8 0 0 0 4 5 4 0 0 0 0 0 0
Cytoplasm 176 122 184 177 233 245 247 261 190 – 161 161 95 158 128 279 170 220 175 94 217 98 114 93
Endoplasmic Reticulum 23 2 14 12 20 27 15 8 5 – 8 5 2 4 1 8 8 5 21 3 24 8 7 24
Endosome 1 1 0 0 1 0 0 1 0 – 0 0 0 0 0 1 2 1 2 2 2 2 4 8
Golgi 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 2 3 1 4 0 0 0 1 2
Mitochondria 0 0 0 10 6 23 46 60 97 – 112 142 5 1 1 6 1 2 1 1 1 0 0 1
Nucleus 3 1 1 4 2 5 14 22 21 – 18 20 2 3 9 20 11 14 11 22 11 19 31 29
Nuclear Periphery 0 0 0 1 1 0 3 1 0 – 2 5 1 1 0 0 0 0 10 5 3 1 4 5
Nucleolus 0 0 1 0 0 0 0 0 1 – 0 0 1 1 0 0 0 0 0 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 0 0 1 6
Vac/Vac Membrane 43 21 64 29 123 90 152 182 134 – 74 152 44 94 123 144 149 131 8 13 14 11 26 22
Unique Cell Count 221 136 249 217 345 358 422 487 394 338 402 139 228 234 390 284 319 245 153 283 150 214 213
Labelled Cell Count 246 147 267 235 388 395 484 541 459 389 498 150 262 263 465 349 378 245 153 283 150 214 213


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.3 9.8 8.1 7.8 9.0 8.7 8.0 7.8 7.5 – 7.1 6.9 9.2 10.1 9.0 10.5 11.8 12.3 9.6 9.5 10.6
Std Deviation (1e-4) 1.6 1.5 1.0 1.4 1.3 1.9 1.5 1.3 1.6 – 1.6 1.5 1.9 1.8 1.6 1.8 2.5 2.0 1.3 1.9 1.9
Intensity Change (Log2) – – – -0.06 0.15 0.09 -0.02 -0.07 -0.11 – -0.19 -0.23 0.18 0.32 0.15 0.36 0.54 0.6 0.23 0.22 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 2.4 0 0 0 0 0 0 0
Cytoplasm 2.0 -1.7 -1.5 -4.0 -5.3 -6.4 – -6.4 -8.4 -1.2 -1.1 -4.4 -0.7 -3.4 -1.3
Endoplasmic Reticulum 0 0.1 0.9 -1.3 -3.0 -3.2 – -2.1 -3.2 -2.0 -2.2 -3.3 -2.4 -1.6 -2.7
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.1 5.4 5.8 8.5 – 10.1 10.6 0 0 0 0 0 0
Nucleus 0 0 0 2.5 3.0 3.3 – 3.3 3.2 0 0 0 3.3 2.7 2.9
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole -3.3 2.6 -0.2 2.8 3.2 2.2 – -1.1 3.2 1.3 3.6 6.1 3.0 6.3 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.5379 10.3779 9.085 9.2464 9.0338 9.6827 8.7196 11.323 9.8615 9.7725 8.3976 9.9727 9.4204 10.187 10.0102 9.1006 9.6949 9.7116
Actin 0.0274 0.0005 0.0034 0.0007 0.0002 0.0032 0.0215 0.0002 0.0075 0.0064 0.0331 0.0068 0.0189 0.0006 0.0059 0.0006 0.0004 0.0002
Bud 0.0007 0.0002 0.0005 0.001 0.0001 0.0001 0.0004 0.0002 0.0002 0.0006 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001
Bud Neck 0.0012 0.0033 0.0016 0.0043 0.0017 0.0026 0.0015 0.0005 0.0011 0.0007 0.0011 0.0021 0.0006 0.0007 0.0004 0.0007 0.0008 0.002
Bud Periphery 0.0011 0.0003 0.0006 0.0036 0.0001 0.0001 0.0004 0 0.0001 0.0008 0.0002 0.0003 0.0001 0.0001 0.0003 0 0 0
Bud Site 0.0027 0.0033 0.0017 0.0007 0.0001 0.0001 0.0013 0.0029 0.0015 0.0023 0.0004 0.0003 0.0008 0.0006 0.0004 0.0001 0.0001 0
Cell Periphery 0.0003 0.0002 0.0002 0.0007 0.0002 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0001 0
Cytoplasm 0.1416 0.342 0.2095 0.2285 0.2982 0.1665 0.2566 0.4188 0.2998 0.2296 0.2722 0.2116 0.1646 0.3432 0.2962 0.3359 0.3685 0.2385
Cytoplasmic Foci 0.0196 0.0004 0.0061 0.0036 0.0005 0.0006 0.0147 0.0006 0.0065 0.0028 0.0118 0.0053 0.0005 0.0005 0.0012 0.0009 0.0013 0.0003
Eisosomes 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0008 0 0 0 0 0
Endoplasmic Reticulum 0.0153 0.0043 0.0188 0.0098 0.0126 0.0047 0.0141 0.0054 0.0078 0.0029 0.0058 0.0058 0.0057 0.0117 0.0041 0.0283 0.036 0.0068
Endosome 0.0582 0.0007 0.0224 0.02 0.0093 0.0027 0.0306 0.0008 0.0051 0.0194 0.0156 0.0139 0.0011 0.0012 0.0037 0.0024 0.0066 0.0011
Golgi 0.0149 0.0002 0.0047 0.0039 0.0009 0.0022 0.0041 0 0.0021 0.01 0.0111 0.0058 0.0007 0 0.0007 0 0.0001 0
Lipid Particles 0.0114 0.0001 0.0083 0.0295 0.003 0.0009 0.0044 0 0.0039 0.0022 0.0442 0.0053 0.0007 0 0.0007 0 0.0002 0
Mitochondria 0.026 0.0005 0.0101 0.0044 0.0164 0.0091 0.0031 0.0002 0.0024 0.0135 0.0104 0.0017 0.001 0.0003 0.0007 0.0003 0.0003 0.0002
None 0.0034 0.0003 0.0009 0.0006 0.0004 0.0002 0.0019 0.0007 0.0022 0.0006 0.0015 0.0008 0.0008 0.0009 0.002 0.0006 0.001 0.0004
Nuclear Periphery 0.1193 0.0674 0.0763 0.0698 0.0571 0.0585 0.0795 0.0389 0.0546 0.0447 0.0377 0.0546 0.1139 0.0897 0.0693 0.0879 0.0666 0.0703
Nucleolus 0.0009 0.0004 0.001 0.0022 0.0005 0.0005 0.001 0.0002 0.0005 0.0003 0.0007 0.0004 0.0002 0.0003 0.0003 0.0001 0.0002 0.0003
Nucleus 0.5291 0.5723 0.6229 0.583 0.5809 0.7426 0.5441 0.5253 0.5937 0.6557 0.513 0.6771 0.685 0.5446 0.6087 0.5351 0.51 0.675
Peroxisomes 0.0037 0.0001 0.0017 0.0008 0.0002 0.0006 0.0031 0 0.0043 0.0011 0.0136 0.0014 0.0012 0 0.0006 0 0 0
Punctate Nuclear 0.0082 0.0015 0.0029 0.003 0.0014 0.002 0.0092 0.0041 0.0047 0.0032 0.0242 0.0044 0.002 0.004 0.003 0.0039 0.0031 0.0031
Vacuole 0.01 0.0018 0.0049 0.0246 0.0119 0.0021 0.0065 0.0007 0.0012 0.0019 0.0018 0.0013 0.0008 0.0011 0.001 0.0023 0.0041 0.0013
Vacuole Periphery 0.0048 0.0003 0.0015 0.0054 0.0041 0.0006 0.002 0.0002 0.0006 0.0011 0.001 0.0006 0.0004 0.0003 0.0004 0.0006 0.0006 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.38 30.4184 28.4049 39.3983 28.2553 37.0655 37.2355 40.117 41.8207 38.7221
Translational Efficiency 0.876 0.9776 1.0168 0.7992 0.8514 0.8592 0.8493 0.7715 0.7425 0.7886

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ubiquitin chain assembly factor (E4); cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3
Localization
Cell Percentages cytoplasm (31%), ER (24%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ufd2

Ufd2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ufd2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available