Standard name
Human Ortholog
Description ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.12 0.07 0.07 0.09 0.18 0.19 0.22 0.18 0.19 0.24 0.09 0.05 0.05 0 0 0 0.1 0.17 0.11 0.07 0.09 0.08
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.26 0.12 0.16 0.21 0.32 0.24 0.24 0.22 0.33 0.33 0.37 0.12 0.05 0.07 0.27 0.28 0.22 0.05 0.07 0 0 0 0
Endoplasmic Reticulum 0.4 0.39 0.17 0.16 0.22 0.34 0.36 0.36 0.32 0.26 0.24 0.28 0.23 0.18 0.21 0.13 0.09 0.1 0.05 0.07 0 0.07 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0
Golgi 0.42 0.35 0.71 0.74 0.54 0.24 0.27 0.27 0.31 0.31 0.27 0.23 0.66 0.76 0.7 0.51 0.53 0.63 0.64 0.48 0.76 0.74 0.76 0.72
Mitochondria 0 0 0.17 0 0 0 0 0 0 0 0.05 0 0 0 0 0.17 0.19 0.11 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0 0.07 0.05 0 0.06 0 0.06 0.07 0 0.06 0.07 0 0 0 0.11 0.06 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 2 5 8 10 11 39 51 46 33 25 47 11 11 14 0 3 2 16 41 8 8 16 19
Bud 1 5 1 3 4 2 9 12 6 7 6 6 2 2 4 2 2 1 2 3 1 2 1 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 0 0 1 0 5 5 1 2 3 4 1 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 11 32 5 18 29 40 51 65 45 59 43 72 15 12 20 35 33 26 8 15 2 0 2 5
Endoplasmic Reticulum 39 49 7 18 31 42 77 95 66 47 31 54 30 41 59 17 11 12 8 16 1 8 6 8
Endosome 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 8 18 1 5 8 9
Golgi 41 44 29 85 76 30 57 73 64 56 35 45 84 178 193 65 63 75 104 117 54 93 151 182
Mitochondria 0 0 7 1 3 5 9 3 9 2 6 7 1 1 0 22 23 13 1 2 0 1 2 1
Nucleus 0 0 0 0 0 0 1 1 0 0 1 0 0 0 1 0 0 0 0 2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 12 1 3 6 6 7 6 16 14 4 8 14 3 5 6 14 7 7 1 0 0 0 1 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 2
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 0 3 0 0 1 1 1 2 4 7 0 4 4 6 5 5 6 3 4 11 0 2 2 6
Unique Cell Count 97 125 41 115 140 124 213 267 205 179 130 196 128 233 275 128 119 119 162 243 72 126 198 253
Labelled Cell Count 109 137 57 139 161 138 256 323 255 217 158 254 151 256 303 160 148 139 162 243 72 126 198 253


Early Golgi, Late Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 73.4 80.7 54.5 50.0 47.4 40.8 38.8 39.7 37.9 37.1 36.0 35.9 64.7 65.8 67.7 80.1 83.3 84.5 76.0 76.0 76.4
Std Deviation (1e-4) 15.5 14.7 12.5 14.6 11.3 12.1 10.2 10.5 10.3 10.1 9.6 8.4 15.4 15.4 15.2 24.6 27.4 22.6 17.7 18.5 22.7
Intensity Change (Log2) – – – -0.12 -0.2 -0.42 -0.49 -0.46 -0.52 -0.56 -0.6 -0.6 0.25 0.27 0.31 0.56 0.61 0.63 0.48 0.48 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0.9 1.1 1.5 1.0 1.0 1.7 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 1.2 2.5 1.7 1.7 1.4 2.6 2.6 3.1 0 0 0 2.0 2.0 1.3
Endoplasmic Reticulum -0.2 0.7 2.0 2.4 2.3 1.9 1.2 0.9 1.4 0.9 0.1 0.6 -0.6 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.4 -1.9 -5.4 -5.4 -5.5 -4.8 -4.7 -5.1 -6.0 -0.6 0.8 -0.1 -2.2 -2.0 -0.9
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0.3 -1.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 64.9031 82.653 76.1146 71.9429 61.5911 76.9498 60.3458 82.1146 79.7066 62.9625 58.8041 78.3149 82.2309 95.0883 89.6803 84.5903 81.9453 89.6754
Actin 0.1124 0.0485 0.0392 0.0585 0.037 0.0622 0.0635 0.099 0.1023 0.0048 0.0144 0.0482 0.0541 0.0564 0.0313 0.0032 0.0045 0.0231
Bud 0.0021 0.0017 0.0019 0.0009 0.0019 0.0005 0.0024 0.0012 0.0007 0.0011 0.0019 0.0002 0.0004 0.0012 0.0006 0 0.0002 0.0002
Bud Neck 0.0051 0.0029 0.0018 0.0024 0.0016 0.0048 0.0015 0.0024 0.0015 0.0006 0.002 0.001 0.0009 0.0022 0.0016 0.0002 0.0005 0.0008
Bud Periphery 0.0026 0.0019 0.0024 0.0026 0.0022 0.0008 0.0028 0.0011 0.0009 0.0012 0.0027 0.0004 0.0005 0.0012 0.0011 0.0001 0.0005 0.0003
Bud Site 0.0092 0.0109 0.0111 0.0011 0.0021 0.0005 0.0052 0.0055 0.0025 0.0036 0.0047 0.0002 0.001 0.0062 0.002 0.0001 0.0002 0.0006
Cell Periphery 0.0105 0.0102 0.0088 0.0099 0.0093 0.0036 0.007 0.0072 0.0125 0.0024 0.0034 0.0033 0.0052 0.0084 0.0081 0.002 0.0035 0.0014
Cytoplasm 0.0082 0.0039 0.0042 0.0008 0.0047 0.0006 0.0107 0.0103 0.0073 0.0299 0.0019 0.0006 0.0008 0.0026 0.0011 0.0004 0.0006 0.0002
Cytoplasmic Foci 0.0456 0.0393 0.0354 0.0131 0.0918 0.0132 0.0472 0.0302 0.0229 0.067 0.0327 0.0122 0.0217 0.0297 0.0172 0.0213 0.017 0.0106
Eisosomes 0.005 0.0013 0.0012 0.0019 0.0007 0.0018 0.0015 0.0008 0.0023 0.0002 0.0003 0.0009 0.0023 0.0012 0.0034 0.0004 0.0003 0.0008
Endoplasmic Reticulum 0.0147 0.0007 0.0063 0.0009 0.0012 0.0007 0.0125 0.0007 0.0032 0.0115 0.0005 0.0006 0.0005 0.0005 0.0005 0.0007 0.0007 0.0004
Endosome 0.0796 0.1058 0.0739 0.0733 0.1199 0.0475 0.0791 0.0862 0.0533 0.0612 0.0792 0.0629 0.0568 0.0779 0.0645 0.0741 0.0875 0.0409
Golgi 0.3814 0.4136 0.4511 0.4933 0.4021 0.6693 0.3711 0.4913 0.4589 0.3602 0.5853 0.6782 0.4598 0.4405 0.4829 0.4084 0.5946 0.6679
Lipid Particles 0.1435 0.2085 0.2233 0.1572 0.1831 0.1344 0.1852 0.1379 0.1791 0.3374 0.0662 0.0871 0.1744 0.1613 0.2211 0.3775 0.1851 0.1804
Mitochondria 0.0866 0.0485 0.0439 0.043 0.0385 0.0342 0.067 0.0397 0.0656 0.0375 0.1577 0.0626 0.1333 0.0994 0.0609 0.0276 0.038 0.05
None 0.0049 0.0002 0.001 0.0001 0.0051 0.0001 0.0057 0.0001 0.0019 0.0024 0.0001 0.0001 0.0003 0.0002 0.0001 0 0.0001 0
Nuclear Periphery 0.0064 0.0007 0.0017 0.0012 0.0008 0.0006 0.0075 0.0008 0.0015 0.006 0.0007 0.0005 0.0011 0.0012 0.0005 0.0005 0.015 0.0002
Nucleolus 0.017 0.0255 0.0201 0.0013 0.028 0.0016 0.0517 0.0334 0.0083 0.0184 0.0119 0.0013 0.036 0.0372 0.0242 0.0051 0.0038 0.0058
Nucleus 0.0028 0.0029 0.0042 0.0006 0.002 0.0004 0.0054 0.0032 0.0015 0.0025 0.0012 0.0002 0.0029 0.0042 0.0023 0.0004 0.0024 0.0003
Peroxisomes 0.0091 0.004 0.0053 0.0026 0.0039 0.0048 0.0096 0.006 0.0036 0.0102 0.0122 0.0011 0.0052 0.0067 0.0035 0.0027 0.0008 0.0015
Punctate Nuclear 0.0013 0.0004 0.0006 0.0001 0.0011 0.0001 0.0115 0.0002 0.0007 0.0034 0.0002 0.0001 0.0007 0.0005 0.0001 0.0001 0.0004 0.0001
Vacuole 0.0273 0.0387 0.0256 0.054 0.0283 0.0066 0.0215 0.0211 0.0237 0.0141 0.0107 0.0103 0.015 0.0234 0.0295 0.0482 0.0125 0.0025
Vacuole Periphery 0.0248 0.0298 0.0372 0.0813 0.0348 0.0115 0.0305 0.0219 0.0457 0.0244 0.01 0.028 0.0271 0.0379 0.0434 0.027 0.0317 0.0118

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 772.9894 760.5575 837.8362 767.2678 817.4926 731.1562 828.82 793.944 845.2746 757.4551
Translational Efficiency 1.3201 1.4058 1.2205 1.7461 1.3532 1.3769 1.4316 1.7049 1.2722 1.3206

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6
Localization
Cell Percentages cytoplasm (14%), nucleus (1%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Arf1

Arf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available