Standard name | |
---|---|
Human Ortholog | |
Description | Anti-silencing protein; causes derepression of silent loci when overexpressed |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 1 | 2 | 5 |
Bud | 0 | 4 | 4 | 3 | 2 | 9 | 7 | 15 | 8 | 7 | 2 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 2 | 4 |
Bud Neck | 0 | 1 | 1 | 1 | 5 | 2 | 2 | 10 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 1 | 1 | 0 | 5 | 0 | 2 | 6 | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 82 | 64 | 64 | 71 | 91 | 111 | 141 | 123 | 124 | 95 | 33 | 85 | 51 | 115 | 121 | 42 | 10 | 8 | 57 | 55 | 34 | 56 | 73 | 74 |
Endoplasmic Reticulum | 3 | 1 | 4 | 1 | 3 | 0 | 0 | 2 | 0 | 0 | 3 | 1 | 0 | 3 | 4 | 10 | 4 | 1 | 6 | 1 | 1 | 2 | 5 | 1 |
Endosome | 6 | 4 | 1 | 3 | 21 | 4 | 2 | 1 | 4 | 1 | 0 | 0 | 12 | 39 | 55 | 50 | 16 | 9 | 72 | 27 | 17 | 13 | 30 | 12 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 3 |
Mitochondria | 156 | 112 | 50 | 58 | 76 | 185 | 274 | 241 | 216 | 197 | 84 | 125 | 19 | 40 | 14 | 25 | 13 | 4 | 49 | 33 | 26 | 19 | 37 | 26 |
Nucleus | 2 | 5 | 4 | 3 | 2 | 6 | 9 | 9 | 11 | 5 | 1 | 6 | 22 | 24 | 9 | 20 | 8 | 6 | 5 | 10 | 6 | 1 | 1 | 1 |
Nuclear Periphery | 3 | 4 | 0 | 1 | 0 | 0 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 11 | 5 | 4 | 0 | 1 | 0 |
Nucleolus | 2 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 5 | 0 | 0 | 1 | 9 | 4 | 3 | 6 | 3 | 2 | 2 | 3 | 0 | 1 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 5 | 3 | 3 | 10 | 27 | 15 | 27 | 21 | 14 | 3 | 0 | 6 | 6 | 12 | 12 | 17 | 5 | 3 | 51 | 43 | 19 | 27 | 37 | 18 |
Vac/Vac Membrane | 30 | 39 | 6 | 8 | 22 | 5 | 9 | 15 | 6 | 12 | 1 | 6 | 24 | 66 | 48 | 31 | 25 | 6 | 21 | 20 | 16 | 8 | 9 | 20 |
Unique Cell Count | 244 | 177 | 119 | 136 | 215 | 293 | 407 | 367 | 312 | 269 | 116 | 195 | 122 | 265 | 228 | 147 | 63 | 32 | 309 | 221 | 149 | 139 | 219 | 181 |
Labelled Cell Count | 290 | 237 | 138 | 160 | 249 | 343 | 475 | 444 | 398 | 322 | 129 | 240 | 145 | 307 | 269 | 203 | 84 | 39 | 309 | 221 | 149 | 139 | 219 | 181 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 3.1 | 3.3 | 3.4 | 3.3 | 3.0 | 2.6 | 2.9 | 2.8 | 2.5 | 2.6 | 2.6 | 4.4 | 4.1 | 4.2 | 5.1 | 5.2 | 5.8 | 4.4 | 4.3 | 4.8 |
Std Deviation (1e-4) | 0.5 | 0.6 | 0.8 | 1.0 | 1.0 | 1.2 | 0.8 | 0.8 | 1.3 | 0.8 | 0.7 | 1.2 | 1.0 | 1.0 | 1.2 | 1.8 | 1.2 | 0.8 | 2.2 | 1.8 | 1.7 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.03 | -0.14 | -0.33 | -0.17 | -0.23 | -0.41 | -0.31 | -0.35 | 0.42 | 0.33 | 0.35 | 0.64 | 0.65 | 0.82 | 0.44 | 0.38 | 0.57 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.8 | 5.9 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.2061 | 2.6226 | 2.4179 | 2.2133 | 1.4821 | 2.1055 | -0.2476 | 0.2246 | 0.2212 | -0.4448 | -0.308 | -0.2733 | 1.2405 | 1.4102 | 1.274 | 0.867 | 1.227 | 0.8977 |
Actin | 0.0366 | 0.002 | 0.0124 | 0.0064 | 0.0012 | 0.0018 | 0.0214 | 0.0013 | 0.0382 | 0.0034 | 0.0017 | 0.0029 | 0.0742 | 0.0022 | 0.0269 | 0.0035 | 0.0215 | 0.0051 |
Bud | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0006 | 0.0115 | 0 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 |
Bud Neck | 0.0022 | 0.0054 | 0.0028 | 0.012 | 0.0083 | 0.0087 | 0.0011 | 0.0007 | 0.0019 | 0.0013 | 0.0009 | 0.0038 | 0.0022 | 0.0008 | 0.0015 | 0.0011 | 0.004 | 0.0069 |
Bud Periphery | 0.0007 | 0.0003 | 0.0003 | 0.0006 | 0.0001 | 0.0002 | 0.0008 | 0.0001 | 0.0008 | 0.0036 | 0 | 0.0002 | 0.0014 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0006 |
Bud Site | 0.0097 | 0.0075 | 0.0034 | 0.0074 | 0.0003 | 0.0007 | 0.0015 | 0.0017 | 0.0117 | 0.0017 | 0.0001 | 0.0003 | 0.0035 | 0.0024 | 0.002 | 0.0005 | 0.0006 | 0.0007 |
Cell Periphery | 0.0003 | 0.0034 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.001 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 |
Cytoplasm | 0.0826 | 0.0956 | 0.0732 | 0.0262 | 0.0546 | 0.0865 | 0.1027 | 0.1173 | 0.0535 | 0.0725 | 0.0896 | 0.076 | 0.0745 | 0.0909 | 0.0752 | 0.089 | 0.1 | 0.1063 |
Cytoplasmic Foci | 0.0689 | 0.0248 | 0.0294 | 0.0431 | 0.0455 | 0.0408 | 0.0584 | 0.0183 | 0.0396 | 0.0338 | 0.0235 | 0.0532 | 0.0811 | 0.1029 | 0.0282 | 0.0416 | 0.0464 | 0.036 |
Eisosomes | 0.0006 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0002 | 0.0013 | 0.0004 | 0.0003 | 0.0002 | 0.001 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0002 |
Endoplasmic Reticulum | 0.0097 | 0.0175 | 0.0119 | 0.0056 | 0.0062 | 0.0081 | 0.0181 | 0.0198 | 0.0121 | 0.0086 | 0.0193 | 0.0185 | 0.0142 | 0.0173 | 0.0145 | 0.0122 | 0.0139 | 0.0115 |
Endosome | 0.0286 | 0.0262 | 0.0211 | 0.0335 | 0.0284 | 0.0242 | 0.0363 | 0.0276 | 0.0511 | 0.0119 | 0.0343 | 0.0448 | 0.0518 | 0.0464 | 0.0317 | 0.0462 | 0.062 | 0.0256 |
Golgi | 0.0054 | 0.0017 | 0.0031 | 0.0061 | 0.0016 | 0.0015 | 0.0047 | 0.002 | 0.0084 | 0.0022 | 0.0007 | 0.0025 | 0.0105 | 0.0034 | 0.0041 | 0.0037 | 0.0066 | 0.0035 |
Lipid Particles | 0.101 | 0.0252 | 0.0479 | 0.0159 | 0.0365 | 0.0211 | 0.0177 | 0.012 | 0.0394 | 0.036 | 0.0122 | 0.0495 | 0.0451 | 0.0407 | 0.0332 | 0.0535 | 0.0683 | 0.0309 |
Mitochondria | 0.0039 | 0.0008 | 0.0011 | 0.0026 | 0.0012 | 0.0012 | 0.0046 | 0.0321 | 0.0235 | 0.0056 | 0.0006 | 0.0025 | 0.0053 | 0.0011 | 0.0016 | 0.0017 | 0.0048 | 0.0042 |
None | 0.2329 | 0.228 | 0.1702 | 0.0814 | 0.1943 | 0.1262 | 0.3659 | 0.3263 | 0.1524 | 0.162 | 0.4875 | 0.2209 | 0.212 | 0.1516 | 0.1437 | 0.0982 | 0.151 | 0.1398 |
Nuclear Periphery | 0.1291 | 0.1249 | 0.1413 | 0.1409 | 0.0991 | 0.0955 | 0.0874 | 0.1176 | 0.2174 | 0.0708 | 0.105 | 0.1291 | 0.1056 | 0.126 | 0.1936 | 0.1268 | 0.1478 | 0.0881 |
Nucleolus | 0.0093 | 0.0195 | 0.0184 | 0.0556 | 0.0141 | 0.0307 | 0.0125 | 0.0191 | 0.0174 | 0.0148 | 0.0031 | 0.0287 | 0.0071 | 0.0089 | 0.0232 | 0.0103 | 0.0167 | 0.0251 |
Nucleus | 0.0435 | 0.1878 | 0.1656 | 0.2389 | 0.1442 | 0.2358 | 0.0834 | 0.1402 | 0.1325 | 0.1903 | 0.0835 | 0.1393 | 0.0741 | 0.1018 | 0.1596 | 0.234 | 0.0714 | 0.1574 |
Peroxisomes | 0.0191 | 0.0026 | 0.0068 | 0.0039 | 0.0047 | 0.0031 | 0.0016 | 0.0009 | 0.0084 | 0.0427 | 0.001 | 0.0031 | 0.0187 | 0.0057 | 0.0116 | 0.0041 | 0.0114 | 0.0039 |
Punctate Nuclear | 0.2092 | 0.219 | 0.2857 | 0.3108 | 0.3558 | 0.3081 | 0.1742 | 0.1544 | 0.1817 | 0.3214 | 0.1252 | 0.2119 | 0.2091 | 0.2916 | 0.2422 | 0.2662 | 0.2664 | 0.3467 |
Vacuole | 0.0045 | 0.0059 | 0.003 | 0.0045 | 0.0022 | 0.0038 | 0.0041 | 0.0059 | 0.0037 | 0.003 | 0.0098 | 0.0085 | 0.0043 | 0.0038 | 0.0035 | 0.0041 | 0.0029 | 0.0046 |
Vacuole Periphery | 0.002 | 0.0016 | 0.0019 | 0.0036 | 0.0014 | 0.0016 | 0.0023 | 0.0022 | 0.004 | 0.0018 | 0.0013 | 0.0038 | 0.0034 | 0.0021 | 0.0029 | 0.0028 | 0.0031 | 0.0022 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.1239 | 6.8759 | 5.1022 | 9.3917 | 24.0249 | 28.6531 | 13.5884 | 8.7447 | 10.7993 | 19.5913 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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