Standard name
Human Ortholog
Description Protein that functions in the cell wall integrity pathway; functions upstream of Pkc1p; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.21 0.19 0.14 0.06 0.15 0.1 0.09 0.06 0 0 0 0 – 0 0 0.05 0.07 0.05 0.12 0.12 0.08 0.13 0.14 0.1
Bud 0.11 0.11 0.05 0 0.07 0 0.07 0 0.07 0.07 0.06 0.07 – 0 0 0 0 0 0.11 0.09 0.06 0.08 0.23 0.08
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.12 0.15 0.15 0.12 0.13 0.07
Bud Site 0 0 0.09 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0.06 0.09 0 0.13 0 0 0 0 0 0 0.05 0.07 – 0.12 0.12 0.18 0.14 0.06 0 0 0 0 0 0
Cytoplasm 0.73 0.73 0.86 0.9 0.79 0.8 0.75 0.81 0.75 0.73 0.64 0.71 – 0.97 0.97 0.83 0.81 0.85 0.54 0.51 0.44 0.56 0.38 0.65
Endoplasmic Reticulum 0 0 0 0 0 0.08 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.1 0.07 0.16 0.23 0.46 0.26 – 0 0 0 0 0 0 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0.06 0 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0.07 0 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 66 53 3 11 58 31 52 32 8 10 9 13 – 1 1 4 9 8 38 37 6 4 19 14
Bud 34 30 1 5 27 9 39 23 17 30 21 35 – 3 0 0 6 6 38 30 4 2 31 11
Bud Neck 1 1 0 2 1 1 5 10 5 4 1 4 – 0 0 0 3 0 39 47 12 4 17 10
Bud Site 8 5 2 1 4 12 15 5 2 9 4 9 – 0 0 0 0 1 – – – – – –
Cell Periphery 20 25 0 23 12 10 20 16 6 13 18 35 – 15 16 14 19 9 8 6 2 0 0 0
Cytoplasm 227 202 19 162 297 254 419 469 182 319 221 350 – 123 125 65 109 128 181 162 35 18 51 93
Endoplasmic Reticulum 6 10 0 4 4 24 11 24 10 6 5 4 – 0 1 3 3 4 8 4 0 0 0 0
Endosome 1 0 0 0 0 2 4 0 1 5 1 3 – 0 0 1 2 2 3 7 2 2 2 3
Golgi 1 2 0 0 0 0 1 0 0 0 0 0 – 0 0 1 3 0 2 4 0 0 0 0
Mitochondria 8 4 0 0 1 12 54 42 39 99 157 131 – 1 1 1 5 6 7 5 7 0 2 2
Nucleus 0 1 0 0 0 0 5 11 1 3 2 1 – 1 0 3 1 1 0 0 1 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 1 0 2 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 1 0 1 – 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 10 0 0 0 4 3 3 1 2 2 0 5 – 0 1 0 0 1 0 0 0 0 0 0
SpindlePole 5 10 1 3 23 14 35 16 2 13 2 22 – 1 0 0 4 3 2 2 0 0 2 0
Vac/Vac Membrane 1 2 0 0 1 1 2 0 4 11 7 6 – 0 1 0 9 3 2 6 3 0 4 3
Unique Cell Count 311 276 22 180 375 318 558 580 243 436 345 495 127 129 78 135 151 337 318 81 33 136 143
Labelled Cell Count 389 345 26 211 432 373 665 651 279 527 448 619 145 146 92 174 172 337 318 81 33 136 143


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 6.7 6.0 6.7 6.7 5.7 5.6 5.6 5.0 4.9 4.5 4.9 – 6.7 6.6 8.7 9.3 7.8 – 6.7 6.9
Std Deviation (1e-4) 0.6 0.9 0.7 1.1 1.1 1.2 1.1 1.0 1.2 1.0 1.0 1.0 – 1.0 0.9 1.6 2.1 1.7 – 1.2 1.3
Intensity Change (Log2) – – – 0.16 0.16 -0.07 -0.08 -0.1 -0.27 -0.3 -0.41 -0.29 – 0.17 0.14 0.54 0.64 0.39 – 0.16 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm 0.5 -0.8 -0.7 -1.2 -0.6 -1.2 -1.4 -2.1 -1.6 – 2.1 2.2 -0.3 -0.6 -0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4257 1.4315 1.1546 1.237 1.0151 1.2614 1.2833 1.774 1.4536 1.201 1.6414 1.7211 -0.2276 0.5051 0.0838 0.381 -0.2607 0.0404
Actin 0.0551 0.0071 0.0354 0.0211 0.0549 0.0211 0.0371 0.0037 0.0313 0.1131 0.0014 0.0028 0.0775 0.0117 0.048 0.0625 0.004 0.0096
Bud 0.0105 0.0253 0.0839 0.0571 0.0135 0.0019 0.0106 0.0012 0.1026 0.0025 0.0005 0.002 0.0167 0.0073 0.0792 0.0365 0.0019 0.0003
Bud Neck 0.0212 0.0048 0.0023 0.0061 0.0018 0.296 0.011 0.0019 0.0095 0.0013 0.001 0.3545 0.0293 0.0049 0.0064 0.0011 0.0009 0.3577
Bud Periphery 0.0232 0.0248 0.1922 0.076 0.0243 0.0042 0.0156 0.0019 0.1765 0.0057 0.0011 0.0035 0.0478 0.0104 0.1416 0.0772 0.0073 0.0005
Bud Site 0.1024 0.0858 0.0221 0.022 0.0053 0.0021 0.0526 0.1141 0.0197 0.0018 0.0019 0.0033 0.0839 0.1582 0.0209 0.003 0.0005 0.0008
Cell Periphery 0.0078 0.0038 0.0053 0.0039 0.0007 0.0029 0.0083 0.0057 0.0036 0.0022 0.0004 0.0011 0.0087 0.0032 0.0045 0.0011 0.0013 0.0011
Cytoplasm 0.4039 0.6445 0.4021 0.5124 0.5546 0.5128 0.5466 0.6594 0.4314 0.7417 0.7016 0.501 0.4387 0.6305 0.4636 0.5205 0.6455 0.4951
Cytoplasmic Foci 0.0498 0.0098 0.0352 0.0377 0.0304 0.031 0.0149 0.0087 0.0155 0.0052 0.0096 0.0066 0.0422 0.0247 0.0164 0.0146 0.021 0.0177
Eisosomes 0.0019 0.0003 0.0004 0.0003 0.0002 0.0006 0.0017 0.0002 0.0003 0.0006 0.0002 0 0.0012 0.0005 0.0007 0.0006 0.0001 0.0003
Endoplasmic Reticulum 0.0094 0.0049 0.0046 0.0053 0.0116 0.002 0.0084 0.0029 0.0039 0.0137 0.0037 0.0028 0.0061 0.0035 0.0062 0.0045 0.0014 0.0009
Endosome 0.0334 0.0031 0.0111 0.0141 0.0648 0.0072 0.0212 0.0014 0.0048 0.0197 0.0453 0.0069 0.0276 0.0037 0.0215 0.0115 0.0036 0.0024
Golgi 0.0091 0.0011 0.0025 0.0038 0.0189 0.0021 0.0081 0.0005 0.003 0.0091 0.0047 0.0018 0.01 0.0015 0.0052 0.0085 0.0017 0.002
Lipid Particles 0.0125 0.001 0.0025 0.004 0.0253 0.0039 0.0179 0.0004 0.0135 0.0057 0.0233 0.0003 0.0139 0.0011 0.0019 0.0093 0.0144 0.001
Mitochondria 0.0206 0.0022 0.0079 0.0029 0.0126 0.0022 0.0092 0.0002 0.0024 0.0094 0.0021 0.0005 0.0161 0.0008 0.0024 0.0109 0.0004 0.0001
None 0.1473 0.1733 0.1748 0.1944 0.1216 0.0989 0.1849 0.1941 0.1633 0.0077 0.1523 0.1092 0.1377 0.1254 0.1677 0.2126 0.2679 0.1072
Nuclear Periphery 0.0174 0.0007 0.0013 0.0041 0.0155 0.001 0.0143 0.0004 0.0007 0.0349 0.0168 0.0004 0.007 0.0027 0.0034 0.0019 0.0004 0.0001
Nucleolus 0.0059 0.0008 0.0008 0.003 0.001 0.0004 0.0022 0.0002 0.0003 0.0008 0.0037 0.0001 0.0007 0.0004 0.0004 0.0003 0.0002 0.0001
Nucleus 0.0125 0.0017 0.0016 0.0063 0.0045 0.001 0.01 0.0009 0.0013 0.0072 0.007 0.0009 0.0056 0.0016 0.0025 0.0018 0.0006 0.0003
Peroxisomes 0.021 0.0005 0.004 0.0013 0.018 0.0044 0.0069 0.0001 0.01 0.005 0.0018 0.0002 0.0188 0.0023 0.0014 0.0051 0.0127 0.0018
Punctate Nuclear 0.0224 0.0019 0.006 0.0084 0.0116 0.0029 0.0137 0.0007 0.0025 0.0072 0.0157 0.0003 0.004 0.0019 0.0022 0.0017 0.0131 0.0004
Vacuole 0.0098 0.0024 0.0033 0.0138 0.0069 0.0012 0.0036 0.0014 0.0032 0.0037 0.0044 0.0016 0.0051 0.0032 0.0033 0.013 0.001 0.0005
Vacuole Periphery 0.0026 0.0002 0.0006 0.002 0.0022 0.0003 0.0011 0.0001 0.0004 0.0017 0.0016 0.0002 0.0013 0.0004 0.0007 0.0017 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.8515 12.6458 10.1657 15.7011 19.4875 10.0147 15.0504 18.3384 16.8926 18.5827
Translational Efficiency 0.885 1.0539 1.4234 0.7891 0.742 1.2543 0.8077 0.6847 0.6884 0.7227

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein that functions in the cell wall integrity pathway; functions upstream of Pkc1p; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
Localization
Cell Percentages cytoplasm (70%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ack1

Ack1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ack1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available