Standard name
Human Ortholog
Description NAD(+)-dependent glutamate dehydrogenase; degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels; genetically interacts with GDH3 by suppressing stress-induced apoptosis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0.1 0 0 0 0.08 0.12 0.16 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.98 1.0 0.97 0.96 0.89 0.88 0.91 0.89 0.84 0.79 0.99 0.98 0.98 0.82 0.81 0.77 0.96 0.96 0.92 0.89 0.86 0.83
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.1 0.09 0.05 0.07 0.12 0.12 0 0 0 0.07 0 0 0 0 0 0 0 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 5 5 4 13 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 2 0 0 0 1 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 1 1 4 3 18 13 15 17 19 32 0 0 4 6 6 13 0 0 0 0 0 0
Cytoplasm 79 188 293 268 333 328 382 383 281 270 247 250 214 251 234 61 42 61 73 179 289 148 204 249
Endoplasmic Reticulum 0 1 3 0 7 16 41 41 16 22 35 38 1 5 1 5 0 3 0 2 8 2 8 18
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 2 0 1 0 0 2 0 0 1 4 0 1 1 1 4 0 0 0 3 0 1 10
Mitochondria 0 0 0 0 0 0 0 1 1 1 0 0 0 0 1 3 0 1 0 0 1 1 1 2
Nucleus 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 1 0 1 0 1 0 2 1 0 2 2 0 0 2 3 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 2
Vac/Vac Membrane 0 1 0 0 0 0 0 0 0 0 1 0 2 0 1 0 3 2 1 1 4 3 4 3
Unique Cell Count 79 190 298 268 345 342 429 435 310 304 295 318 217 255 240 74 52 79 76 187 314 167 238 301
Labelled Cell Count 79 192 300 269 346 347 443 442 321 317 308 339 220 257 242 78 58 84 76 187 314 167 238 301


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 187.4 175.1 146.0 149.1 145.4 127.8 118.8 101.6 90.0 87.4 81.0 73.1 167.8 150.8 130.9 266.1 294.6 310.9 182.7 164.6 185.1
Std Deviation (1e-4) 46.0 42.6 40.8 38.5 40.0 33.2 30.6 30.9 29.5 24.9 22.1 21.5 52.2 40.0 35.9 73.3 105.8 82.3 54.5 52.8 46.5
Intensity Change (Log2) 0.03 -0.01 -0.19 -0.3 -0.52 -0.7 -0.74 -0.85 -1.0 0.2 0.05 -0.16 0.87 1.01 1.09 0.32 0.17 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.5 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.2 2.6 3.5 3.8 4.1 5.4 0 0 0 0 0 0
Cytoplasm 2.1 -1.4 -1.8 -4.8 -5.1 -4.1 -4.7 -6.2 -7.6 0.3 0.1 -0.7 -5.7 -5.8 -7.0
Endoplasmic Reticulum 0 0 2.7 4.8 4.7 2.9 3.8 5.4 5.4 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.0158 19.7678 16.9113 19.7561 13.8152 17.5277 14.6878 21.0788 18.4719 16.6614 17.8009 17.727 15.4665 24.5547 18.0097 17.4911 16.4462 19.926
Actin 0.0332 0.0004 0.0182 0.0045 0.0097 0.0034 0.0088 0.0002 0.0037 0.0014 0.0125 0.0022 0.0063 0.0013 0.0076 0.0005 0.0056 0.0093
Bud 0.0021 0.0015 0.0022 0.0021 0.0016 0.0009 0.0009 0.0005 0.0018 0.0018 0.0123 0.0008 0.0016 0.0015 0.0017 0.0015 0.0008 0.0005
Bud Neck 0.0013 0.0005 0.0027 0.0008 0.0034 0.0019 0.0031 0.0002 0.0007 0.001 0.0007 0.0011 0.0004 0.0004 0.0008 0.0006 0.0008 0.0014
Bud Periphery 0.0029 0.0007 0.0026 0.0018 0.0028 0.0011 0.0006 0.0001 0.0023 0.001 0.0077 0.0015 0.0027 0.0006 0.0007 0.0005 0.0004 0.0003
Bud Site 0.0042 0.0019 0.0068 0.0011 0.0076 0.0004 0.0056 0.0006 0.002 0.003 0.0019 0.0003 0.0021 0.0025 0.0014 0.0019 0.0011 0.0001
Cell Periphery 0.0026 0.002 0.0014 0.0006 0.0006 0.0005 0.0006 0.0001 0.0003 0.0005 0.0002 0.0004 0.0006 0.0006 0.0006 0.0002 0.0001 0.0002
Cytoplasm 0.7204 0.928 0.8479 0.7604 0.6438 0.8965 0.8039 0.9573 0.8668 0.7699 0.8517 0.9013 0.842 0.8876 0.8962 0.8645 0.8418 0.9133
Cytoplasmic Foci 0.012 0.0045 0.007 0.0148 0.0385 0.0093 0.0278 0.0052 0.0138 0.0239 0.0145 0.0078 0.0168 0.0274 0.0165 0.0139 0.0275 0.0079
Eisosomes 0.001 0 0.0003 0.0002 0.0005 0.0001 0.0001 0 0 0 0.0001 0 0.0001 0 0.0002 0 0 0
Endoplasmic Reticulum 0.0472 0.01 0.0198 0.0065 0.0228 0.0229 0.0267 0.0042 0.0123 0.0085 0.0049 0.0183 0.0346 0.0031 0.0129 0.0313 0.0201 0.0128
Endosome 0.0333 0.0056 0.0185 0.0216 0.0537 0.0139 0.0458 0.006 0.0328 0.0845 0.0426 0.0283 0.0266 0.0275 0.0175 0.0478 0.0631 0.0315
Golgi 0.007 0.0006 0.0022 0.0017 0.0082 0.0026 0.0093 0.0008 0.0045 0.0044 0.0048 0.0055 0.0073 0.0031 0.0028 0.0028 0.006 0.0055
Lipid Particles 0.0071 0.0001 0.0013 0.005 0.0189 0.0004 0.0021 0 0.0019 0.001 0.0069 0.0014 0.0107 0.0023 0.0032 0.0005 0.003 0.0001
Mitochondria 0.0179 0.0003 0.0081 0.013 0.066 0.0081 0.0026 0.0003 0.0077 0.0028 0.0018 0.0029 0.0043 0.002 0.0011 0.0005 0.0072 0.0054
None 0.012 0.0007 0.0009 0.0047 0.0105 0.0005 0.0052 0.0005 0.0012 0.0023 0.0017 0.0006 0.005 0.0011 0.0036 0.0007 0.0031 0.0005
Nuclear Periphery 0.0124 0.0018 0.0041 0.0064 0.0091 0.0025 0.0093 0.0018 0.0062 0.0063 0.0041 0.0027 0.0044 0.0009 0.0028 0.005 0.0047 0.001
Nucleolus 0.0005 0.0001 0.0021 0.0109 0.0024 0.0001 0.0007 0 0.0001 0.0005 0.0002 0.0002 0.0002 0.0003 0.0012 0.0001 0.0001 0
Nucleus 0.0058 0.0021 0.0039 0.0046 0.0066 0.0022 0.0045 0.0016 0.0028 0.0083 0.0017 0.002 0.0029 0.0024 0.0023 0.0016 0.002 0.0011
Peroxisomes 0.0032 0.0001 0.0005 0.0013 0.0088 0.0025 0.0024 0.0001 0.0048 0.0007 0.0021 0.0003 0.0084 0.0004 0.0004 0.0001 0.0013 0.0002
Punctate Nuclear 0.0011 0.0001 0.0003 0.0009 0.007 0.0001 0.0073 0 0.001 0.0029 0.0025 0.0016 0.0008 0.0002 0.0018 0.0002 0.001 0.0001
Vacuole 0.0599 0.0363 0.0439 0.1183 0.065 0.0239 0.028 0.0189 0.03 0.0655 0.02 0.0183 0.0192 0.0312 0.0222 0.0212 0.0074 0.0064
Vacuole Periphery 0.0128 0.0027 0.0054 0.0189 0.0126 0.0063 0.0046 0.0014 0.0034 0.0099 0.0051 0.0026 0.003 0.0035 0.0026 0.0045 0.0028 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.9141 27.9759 22.5451 23.3694 18.9788 48.0938 28.8947 31.3058 31.5576 32.1669
Translational Efficiency 0.9378 0.8103 0.6282 0.7192 0.7289 0.8589 0.9349 0.6573 0.6572 0.7013

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description NAD(+)-dependent glutamate dehydrogenase; degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels; genetically interacts with GDH3 by suppressing stress-induced apoptosis
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Gdh2

Gdh2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gdh2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available