Standard name | |
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Human Ortholog | |
Description | NAD(+)-dependent glutamate dehydrogenase; degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels; genetically interacts with GDH3 by suppressing stress-induced apoptosis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 5 | 4 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 3 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 1 | 4 | 3 | 18 | 13 | 15 | 17 | 19 | 32 | 0 | 0 | 4 | 6 | 6 | 13 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 79 | 188 | 293 | 268 | 333 | 328 | 382 | 383 | 281 | 270 | 247 | 250 | 214 | 251 | 234 | 61 | 42 | 61 | 73 | 179 | 289 | 148 | 204 | 249 |
Endoplasmic Reticulum | 0 | 1 | 3 | 0 | 7 | 16 | 41 | 41 | 16 | 22 | 35 | 38 | 1 | 5 | 1 | 5 | 0 | 3 | 0 | 2 | 8 | 2 | 8 | 18 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 4 | 0 | 1 | 1 | 1 | 4 | 0 | 0 | 0 | 3 | 0 | 1 | 10 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 2 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 2 | 2 | 0 | 0 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 0 | 3 | 2 | 1 | 1 | 4 | 3 | 4 | 3 |
Unique Cell Count | 79 | 190 | 298 | 268 | 345 | 342 | 429 | 435 | 310 | 304 | 295 | 318 | 217 | 255 | 240 | 74 | 52 | 79 | 76 | 187 | 314 | 167 | 238 | 301 |
Labelled Cell Count | 79 | 192 | 300 | 269 | 346 | 347 | 443 | 442 | 321 | 317 | 308 | 339 | 220 | 257 | 242 | 78 | 58 | 84 | 76 | 187 | 314 | 167 | 238 | 301 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 187.4 | 175.1 | 146.0 | 149.1 | 145.4 | 127.8 | 118.8 | 101.6 | 90.0 | 87.4 | 81.0 | 73.1 | 167.8 | 150.8 | 130.9 | 266.1 | 294.6 | 310.9 | 182.7 | 164.6 | 185.1 |
Std Deviation (1e-4) | 46.0 | 42.6 | 40.8 | 38.5 | 40.0 | 33.2 | 30.6 | 30.9 | 29.5 | 24.9 | 22.1 | 21.5 | 52.2 | 40.0 | 35.9 | 73.3 | 105.8 | 82.3 | 54.5 | 52.8 | 46.5 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.01 | -0.19 | -0.3 | -0.52 | -0.7 | -0.74 | -0.85 | -1.0 | 0.2 | 0.05 | -0.16 | 0.87 | 1.01 | 1.09 | 0.32 | 0.17 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 3.2 | 2.6 | 3.5 | 3.8 | 4.1 | 5.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.1 | -1.4 | -1.8 | -4.8 | -5.1 | -4.1 | -4.7 | -6.2 | -7.6 | 0.3 | 0.1 | -0.7 | -5.7 | -5.8 | -7.0 |
Endoplasmic Reticulum | 0 | 0 | 2.7 | 4.8 | 4.7 | 2.9 | 3.8 | 5.4 | 5.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.0158 | 19.7678 | 16.9113 | 19.7561 | 13.8152 | 17.5277 | 14.6878 | 21.0788 | 18.4719 | 16.6614 | 17.8009 | 17.727 | 15.4665 | 24.5547 | 18.0097 | 17.4911 | 16.4462 | 19.926 |
Actin | 0.0332 | 0.0004 | 0.0182 | 0.0045 | 0.0097 | 0.0034 | 0.0088 | 0.0002 | 0.0037 | 0.0014 | 0.0125 | 0.0022 | 0.0063 | 0.0013 | 0.0076 | 0.0005 | 0.0056 | 0.0093 |
Bud | 0.0021 | 0.0015 | 0.0022 | 0.0021 | 0.0016 | 0.0009 | 0.0009 | 0.0005 | 0.0018 | 0.0018 | 0.0123 | 0.0008 | 0.0016 | 0.0015 | 0.0017 | 0.0015 | 0.0008 | 0.0005 |
Bud Neck | 0.0013 | 0.0005 | 0.0027 | 0.0008 | 0.0034 | 0.0019 | 0.0031 | 0.0002 | 0.0007 | 0.001 | 0.0007 | 0.0011 | 0.0004 | 0.0004 | 0.0008 | 0.0006 | 0.0008 | 0.0014 |
Bud Periphery | 0.0029 | 0.0007 | 0.0026 | 0.0018 | 0.0028 | 0.0011 | 0.0006 | 0.0001 | 0.0023 | 0.001 | 0.0077 | 0.0015 | 0.0027 | 0.0006 | 0.0007 | 0.0005 | 0.0004 | 0.0003 |
Bud Site | 0.0042 | 0.0019 | 0.0068 | 0.0011 | 0.0076 | 0.0004 | 0.0056 | 0.0006 | 0.002 | 0.003 | 0.0019 | 0.0003 | 0.0021 | 0.0025 | 0.0014 | 0.0019 | 0.0011 | 0.0001 |
Cell Periphery | 0.0026 | 0.002 | 0.0014 | 0.0006 | 0.0006 | 0.0005 | 0.0006 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0006 | 0.0006 | 0.0006 | 0.0002 | 0.0001 | 0.0002 |
Cytoplasm | 0.7204 | 0.928 | 0.8479 | 0.7604 | 0.6438 | 0.8965 | 0.8039 | 0.9573 | 0.8668 | 0.7699 | 0.8517 | 0.9013 | 0.842 | 0.8876 | 0.8962 | 0.8645 | 0.8418 | 0.9133 |
Cytoplasmic Foci | 0.012 | 0.0045 | 0.007 | 0.0148 | 0.0385 | 0.0093 | 0.0278 | 0.0052 | 0.0138 | 0.0239 | 0.0145 | 0.0078 | 0.0168 | 0.0274 | 0.0165 | 0.0139 | 0.0275 | 0.0079 |
Eisosomes | 0.001 | 0 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0472 | 0.01 | 0.0198 | 0.0065 | 0.0228 | 0.0229 | 0.0267 | 0.0042 | 0.0123 | 0.0085 | 0.0049 | 0.0183 | 0.0346 | 0.0031 | 0.0129 | 0.0313 | 0.0201 | 0.0128 |
Endosome | 0.0333 | 0.0056 | 0.0185 | 0.0216 | 0.0537 | 0.0139 | 0.0458 | 0.006 | 0.0328 | 0.0845 | 0.0426 | 0.0283 | 0.0266 | 0.0275 | 0.0175 | 0.0478 | 0.0631 | 0.0315 |
Golgi | 0.007 | 0.0006 | 0.0022 | 0.0017 | 0.0082 | 0.0026 | 0.0093 | 0.0008 | 0.0045 | 0.0044 | 0.0048 | 0.0055 | 0.0073 | 0.0031 | 0.0028 | 0.0028 | 0.006 | 0.0055 |
Lipid Particles | 0.0071 | 0.0001 | 0.0013 | 0.005 | 0.0189 | 0.0004 | 0.0021 | 0 | 0.0019 | 0.001 | 0.0069 | 0.0014 | 0.0107 | 0.0023 | 0.0032 | 0.0005 | 0.003 | 0.0001 |
Mitochondria | 0.0179 | 0.0003 | 0.0081 | 0.013 | 0.066 | 0.0081 | 0.0026 | 0.0003 | 0.0077 | 0.0028 | 0.0018 | 0.0029 | 0.0043 | 0.002 | 0.0011 | 0.0005 | 0.0072 | 0.0054 |
None | 0.012 | 0.0007 | 0.0009 | 0.0047 | 0.0105 | 0.0005 | 0.0052 | 0.0005 | 0.0012 | 0.0023 | 0.0017 | 0.0006 | 0.005 | 0.0011 | 0.0036 | 0.0007 | 0.0031 | 0.0005 |
Nuclear Periphery | 0.0124 | 0.0018 | 0.0041 | 0.0064 | 0.0091 | 0.0025 | 0.0093 | 0.0018 | 0.0062 | 0.0063 | 0.0041 | 0.0027 | 0.0044 | 0.0009 | 0.0028 | 0.005 | 0.0047 | 0.001 |
Nucleolus | 0.0005 | 0.0001 | 0.0021 | 0.0109 | 0.0024 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0012 | 0.0001 | 0.0001 | 0 |
Nucleus | 0.0058 | 0.0021 | 0.0039 | 0.0046 | 0.0066 | 0.0022 | 0.0045 | 0.0016 | 0.0028 | 0.0083 | 0.0017 | 0.002 | 0.0029 | 0.0024 | 0.0023 | 0.0016 | 0.002 | 0.0011 |
Peroxisomes | 0.0032 | 0.0001 | 0.0005 | 0.0013 | 0.0088 | 0.0025 | 0.0024 | 0.0001 | 0.0048 | 0.0007 | 0.0021 | 0.0003 | 0.0084 | 0.0004 | 0.0004 | 0.0001 | 0.0013 | 0.0002 |
Punctate Nuclear | 0.0011 | 0.0001 | 0.0003 | 0.0009 | 0.007 | 0.0001 | 0.0073 | 0 | 0.001 | 0.0029 | 0.0025 | 0.0016 | 0.0008 | 0.0002 | 0.0018 | 0.0002 | 0.001 | 0.0001 |
Vacuole | 0.0599 | 0.0363 | 0.0439 | 0.1183 | 0.065 | 0.0239 | 0.028 | 0.0189 | 0.03 | 0.0655 | 0.02 | 0.0183 | 0.0192 | 0.0312 | 0.0222 | 0.0212 | 0.0074 | 0.0064 |
Vacuole Periphery | 0.0128 | 0.0027 | 0.0054 | 0.0189 | 0.0126 | 0.0063 | 0.0046 | 0.0014 | 0.0034 | 0.0099 | 0.0051 | 0.0026 | 0.003 | 0.0035 | 0.0026 | 0.0045 | 0.0028 | 0.0023 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 43.9141 | 27.9759 | 22.5451 | 23.3694 | 18.9788 | 48.0938 | 28.8947 | 31.3058 | 31.5576 | 32.1669 |
Translational Efficiency | 0.9378 | 0.8103 | 0.6282 | 0.7192 | 0.7289 | 0.8589 | 0.9349 | 0.6573 | 0.6572 | 0.7013 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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