Standard name | |
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Human Ortholog | |
Description | Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 2 | 2 | 9 | – | 3 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud | 0 | 1 | 0 | 1 | 0 | 1 | 2 | – | 3 | 2 | 6 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 23 | 9 | 9 | 3 | 10 | 10 | – | 5 | 10 | 2 | 8 | 3 | 3 | 11 | 14 | 23 | 24 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 240 | 526 | 272 | 245 | 270 | 309 | 336 | – | 168 | 236 | 169 | 196 | 192 | 161 | 272 | 268 | 296 | 276 | 230 | 541 | 234 | 154 | 172 | 194 |
Endoplasmic Reticulum | 3 | 16 | 2 | 2 | 6 | 8 | 12 | – | 7 | 28 | 14 | 15 | 2 | 4 | 1 | 11 | 15 | 11 | 3 | 10 | 13 | 0 | 2 | 5 |
Endosome | 0 | 0 | 0 | 1 | 0 | 0 | 2 | – | 1 | 2 | 0 | 1 | 0 | 2 | 0 | 8 | 1 | 4 | 3 | 3 | 0 | 0 | 0 | 2 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 3 | 3 | 1 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 5 | 0 | 0 | 4 | 5 | 30 | 45 | – | 69 | 63 | 140 | 82 | 0 | 0 | 0 | 8 | 6 | 1 | 2 | 2 | 10 | 2 | 4 | 7 |
Nucleus | 0 | 2 | 1 | 0 | 4 | 3 | 2 | – | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 2 | 3 | 4 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 2 | 0 | 5 | 9 | 10 | – | 14 | 12 | 7 | 11 | 0 | 0 | 2 | 20 | 32 | 10 | 0 | 12 | 4 | 3 | 1 | 3 |
Unique Cell Count | 243 | 543 | 274 | 249 | 286 | 345 | 396 | 244 | 319 | 287 | 285 | 194 | 168 | 276 | 298 | 324 | 294 | 247 | 580 | 272 | 163 | 189 | 223 | |
Labelled Cell Count | 253 | 570 | 286 | 262 | 295 | 373 | 429 | 272 | 357 | 342 | 322 | 200 | 171 | 287 | 333 | 379 | 336 | 247 | 580 | 272 | 163 | 189 | 223 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 9.4 | 8.3 | 7.8 | 8.6 | 7.3 | 7.2 | – | 6.4 | 6.6 | 5.9 | 6.4 | 8.5 | 8.5 | 8.4 | 8.8 | 8.9 | 8.9 | 7.6 | 7.8 | 7.7 |
Std Deviation (1e-4) | 0.7 | 1.1 | 0.9 | 1.1 | 1.6 | 1.3 | 1.2 | – | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.1 | 1.6 | 1.6 | 1.6 | 0.9 | 1.0 | 1.0 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.04 | -0.18 | -0.2 | – | -0.38 | -0.32 | -0.49 | -0.37 | 0.04 | 0.03 | 0.02 | 0.08 | 0.1 | 0.09 | -0.13 | -0.09 | -0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.2 | -1.8 | -0.3 | -0.6 | – | -0.9 | -0.1 | -2.2 | -0.3 | 0 | 0 | 0.4 | 0.9 | 2.1 | 2.5 |
Cytoplasm | -0.9 | -3.3 | -5.0 | -6.3 | – | -9.7 | -8.8 | -11.7 | -9.8 | -0.3 | -2.5 | -0.8 | -4.9 | -4.4 | -3.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.0 | – | 0 | 4.5 | 3.0 | 3.1 | 0 | 0 | 0 | 2.4 | 2.9 | 2.4 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 5.0 | 5.8 | – | 9.5 | 7.8 | 13.3 | 9.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 3.3 | 2.4 | 0 | 2.5 | 0 | 0 | 0 | 3.7 | 4.8 | 2.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.1092 | 3.0233 | 2.6469 | 2.1464 | 2.1262 | 2.6609 | 2.7997 | 3.8009 | 3.4522 | 3.286 | 3.6019 | 3.6613 | 3.2894 | 4.122 | 3.637 | 3.3899 | 3.4182 | 3.6184 |
Actin | 0.015 | 0.0008 | 0.0121 | 0.0066 | 0.0116 | 0.0014 | 0.0168 | 0.0011 | 0.0065 | 0.0167 | 0.0058 | 0.0021 | 0.023 | 0.0004 | 0.0219 | 0.0052 | 0.004 | 0.0065 |
Bud | 0.0004 | 0.0004 | 0.0005 | 0.0003 | 0.0061 | 0.0002 | 0.0005 | 0.0013 | 0.0004 | 0.0007 | 0.0003 | 0.0002 | 0.0009 | 0.0003 | 0.0009 | 0.0004 | 0.0291 | 0.0002 |
Bud Neck | 0.0007 | 0.0003 | 0.0009 | 0.0004 | 0.002 | 0.0009 | 0.0026 | 0.0002 | 0.0004 | 0.001 | 0.0004 | 0.0006 | 0.0027 | 0.0002 | 0.0011 | 0.0033 | 0.0003 | 0.0011 |
Bud Periphery | 0.0005 | 0.0003 | 0.0005 | 0.0001 | 0.008 | 0.0001 | 0.0007 | 0.0006 | 0.001 | 0.0007 | 0.0001 | 0.0002 | 0.0013 | 0.0001 | 0.0008 | 0.0002 | 0.0228 | 0.0002 |
Bud Site | 0.0016 | 0.0037 | 0.0041 | 0.0003 | 0.0066 | 0.0002 | 0.0062 | 0.0089 | 0.0008 | 0.0014 | 0.0003 | 0.0001 | 0.0052 | 0.0013 | 0.0038 | 0.0015 | 0.0027 | 0.0002 |
Cell Periphery | 0.0005 | 0.0004 | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0012 | 0.0004 | 0.0007 | 0.0003 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.0009 | 0.0003 | 0.0001 |
Cytoplasm | 0.7595 | 0.9162 | 0.8189 | 0.7966 | 0.8485 | 0.8931 | 0.739 | 0.9108 | 0.8694 | 0.666 | 0.7361 | 0.8877 | 0.7792 | 0.9562 | 0.8517 | 0.856 | 0.7997 | 0.9087 |
Cytoplasmic Foci | 0.0363 | 0.0124 | 0.0202 | 0.0407 | 0.0291 | 0.0202 | 0.0232 | 0.0142 | 0.0227 | 0.0435 | 0.0239 | 0.0191 | 0.0336 | 0.0118 | 0.0236 | 0.0304 | 0.0163 | 0.0143 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0272 | 0.0099 | 0.0066 | 0.003 | 0.0084 | 0.0046 | 0.0437 | 0.0123 | 0.0157 | 0.033 | 0.0109 | 0.0095 | 0.0365 | 0.0069 | 0.0124 | 0.0216 | 0.0252 | 0.0217 |
Endosome | 0.0613 | 0.0196 | 0.0425 | 0.105 | 0.0399 | 0.034 | 0.0507 | 0.0099 | 0.0249 | 0.0857 | 0.0685 | 0.0374 | 0.043 | 0.0127 | 0.0374 | 0.0522 | 0.0262 | 0.0265 |
Golgi | 0.0194 | 0.0024 | 0.0204 | 0.0054 | 0.0069 | 0.004 | 0.0083 | 0.0018 | 0.0075 | 0.0282 | 0.0253 | 0.0115 | 0.0074 | 0.0016 | 0.0047 | 0.0072 | 0.0043 | 0.0032 |
Lipid Particles | 0.0128 | 0.0002 | 0.0051 | 0.0064 | 0.0016 | 0.0004 | 0.0053 | 0.0007 | 0.0015 | 0.0199 | 0.0132 | 0.0005 | 0.0052 | 0.0001 | 0.0013 | 0.0009 | 0.0008 | 0.0017 |
Mitochondria | 0.0069 | 0.001 | 0.0225 | 0.0011 | 0.0057 | 0.0015 | 0.0055 | 0.0004 | 0.0069 | 0.0189 | 0.0047 | 0.0104 | 0.0031 | 0.0004 | 0.003 | 0.001 | 0.0537 | 0.002 |
None | 0.0332 | 0.0275 | 0.024 | 0.0038 | 0.0086 | 0.0231 | 0.062 | 0.029 | 0.0271 | 0.02 | 0.0061 | 0.0153 | 0.0313 | 0.004 | 0.0166 | 0.0088 | 0.0069 | 0.0045 |
Nuclear Periphery | 0.0052 | 0.0006 | 0.001 | 0.0014 | 0.0023 | 0.0104 | 0.0116 | 0.0008 | 0.0011 | 0.0075 | 0.0341 | 0.0009 | 0.0074 | 0.0008 | 0.0025 | 0.0017 | 0.0013 | 0.0046 |
Nucleolus | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0 | 0 | 0.0002 | 0.0002 | 0 | 0.0013 | 0 | 0.001 | 0 | 0.0001 | 0.0001 |
Nucleus | 0.0017 | 0.0008 | 0.0016 | 0.001 | 0.0029 | 0.0019 | 0.0059 | 0.001 | 0.0008 | 0.0018 | 0.0395 | 0.0009 | 0.0029 | 0.0007 | 0.0037 | 0.0012 | 0.0011 | 0.0011 |
Peroxisomes | 0.0084 | 0.0001 | 0.0048 | 0.0143 | 0.0007 | 0.0003 | 0.0011 | 0.0033 | 0.0083 | 0.0395 | 0.008 | 0.0003 | 0.0045 | 0.0001 | 0.0008 | 0.0002 | 0.0023 | 0.0006 |
Punctate Nuclear | 0.0019 | 0.0002 | 0.006 | 0.0019 | 0.0009 | 0.0003 | 0.0048 | 0.0002 | 0.0004 | 0.0043 | 0.0069 | 0.0003 | 0.0023 | 0.0001 | 0.0062 | 0.0004 | 0.0004 | 0.0007 |
Vacuole | 0.0051 | 0.0027 | 0.0055 | 0.0101 | 0.0071 | 0.002 | 0.0076 | 0.0027 | 0.003 | 0.0076 | 0.008 | 0.0018 | 0.0066 | 0.0017 | 0.0053 | 0.0056 | 0.0016 | 0.0013 |
Vacuole Periphery | 0.0021 | 0.0005 | 0.0012 | 0.0014 | 0.0023 | 0.0014 | 0.0017 | 0.0003 | 0.0007 | 0.0028 | 0.0074 | 0.0008 | 0.0015 | 0.0004 | 0.0012 | 0.0013 | 0.0006 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 25.1803 | 20.2334 | 23.6471 | 31.8745 | 22.3762 | 15.835 | 30.9426 | 24.1635 | 23.4244 | 33.9641 |
Translational Efficiency | 1.1084 | 1.4257 | 1.0962 | 0.7381 | 1.0946 | 1.9118 | 0.9536 | 1.4325 | 1.4098 | 0.8981 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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