Standard name
Human Ortholog
Description Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.08 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.99 0.98 0.94 0.9 0.85 0.69 0.74 0.59 0.69 0.99 0.96 0.99 0.9 0.91 0.94 0.93 0.93 0.86 0.95 0.91 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.09 0.05 0.05 0 0 0 0 0.05 0 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.11 0.28 0.2 0.49 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.07 0.1 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 2 2 9 3 2 1 1 1 1 0 0 0 2 0 1 0 0 0 1
Bud 0 1 0 1 0 1 2 3 2 6 7 1 0 0 0 0 0 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 4 23 9 9 3 10 10 5 10 2 8 3 3 11 14 23 24 0 0 0 0 0 0
Cytoplasm 240 526 272 245 270 309 336 168 236 169 196 192 161 272 268 296 276 230 541 234 154 172 194
Endoplasmic Reticulum 3 16 2 2 6 8 12 7 28 14 15 2 4 1 11 15 11 3 10 13 0 2 5
Endosome 0 0 0 1 0 0 2 1 2 0 1 0 2 0 8 1 4 3 3 0 0 0 2
Golgi 1 0 0 0 0 0 0 0 0 0 1 0 0 1 1 3 3 1 1 0 0 1 0
Mitochondria 5 0 0 4 5 30 45 69 63 140 82 0 0 0 8 6 1 2 2 10 2 4 7
Nucleus 0 2 1 0 4 3 2 0 0 3 0 1 0 0 2 3 4 0 1 0 0 0 1
Nuclear Periphery 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 0 1 2 0 5 9 10 14 12 7 11 0 0 2 20 32 10 0 12 4 3 1 3
Unique Cell Count 243 543 274 249 286 345 396 244 319 287 285 194 168 276 298 324 294 247 580 272 163 189 223
Labelled Cell Count 253 570 286 262 295 373 429 272 357 342 322 200 171 287 333 379 336 247 580 272 163 189 223


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 9.4 8.3 7.8 8.6 7.3 7.2 6.4 6.6 5.9 6.4 8.5 8.5 8.4 8.8 8.9 8.9 7.6 7.8 7.7
Std Deviation (1e-4) 0.7 1.1 0.9 1.1 1.6 1.3 1.2 1.0 1.0 1.0 1.0 1.0 1.0 1.1 1.6 1.6 1.6 0.9 1.0 1.0
Intensity Change (Log2) -0.08 0.04 -0.18 -0.2 -0.38 -0.32 -0.49 -0.37 0.04 0.03 0.02 0.08 0.1 0.09 -0.13 -0.09 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.2 -1.8 -0.3 -0.6 -0.9 -0.1 -2.2 -0.3 0 0 0.4 0.9 2.1 2.5
Cytoplasm -0.9 -3.3 -5.0 -6.3 -9.7 -8.8 -11.7 -9.8 -0.3 -2.5 -0.8 -4.9 -4.4 -3.5
Endoplasmic Reticulum 0 0 0 2.0 0 4.5 3.0 3.1 0 0 0 2.4 2.9 2.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.0 5.8 9.5 7.8 13.3 9.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.3 2.4 0 2.5 0 0 0 3.7 4.8 2.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1092 3.0233 2.6469 2.1464 2.1262 2.6609 2.7997 3.8009 3.4522 3.286 3.6019 3.6613 3.2894 4.122 3.637 3.3899 3.4182 3.6184
Actin 0.015 0.0008 0.0121 0.0066 0.0116 0.0014 0.0168 0.0011 0.0065 0.0167 0.0058 0.0021 0.023 0.0004 0.0219 0.0052 0.004 0.0065
Bud 0.0004 0.0004 0.0005 0.0003 0.0061 0.0002 0.0005 0.0013 0.0004 0.0007 0.0003 0.0002 0.0009 0.0003 0.0009 0.0004 0.0291 0.0002
Bud Neck 0.0007 0.0003 0.0009 0.0004 0.002 0.0009 0.0026 0.0002 0.0004 0.001 0.0004 0.0006 0.0027 0.0002 0.0011 0.0033 0.0003 0.0011
Bud Periphery 0.0005 0.0003 0.0005 0.0001 0.008 0.0001 0.0007 0.0006 0.001 0.0007 0.0001 0.0002 0.0013 0.0001 0.0008 0.0002 0.0228 0.0002
Bud Site 0.0016 0.0037 0.0041 0.0003 0.0066 0.0002 0.0062 0.0089 0.0008 0.0014 0.0003 0.0001 0.0052 0.0013 0.0038 0.0015 0.0027 0.0002
Cell Periphery 0.0005 0.0004 0.0005 0.0001 0.0004 0.0001 0.0012 0.0004 0.0007 0.0003 0.0001 0.0001 0.0008 0.0002 0.0002 0.0009 0.0003 0.0001
Cytoplasm 0.7595 0.9162 0.8189 0.7966 0.8485 0.8931 0.739 0.9108 0.8694 0.666 0.7361 0.8877 0.7792 0.9562 0.8517 0.856 0.7997 0.9087
Cytoplasmic Foci 0.0363 0.0124 0.0202 0.0407 0.0291 0.0202 0.0232 0.0142 0.0227 0.0435 0.0239 0.0191 0.0336 0.0118 0.0236 0.0304 0.0163 0.0143
Eisosomes 0.0002 0 0.0002 0 0 0 0.0005 0 0.0001 0.0002 0 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0272 0.0099 0.0066 0.003 0.0084 0.0046 0.0437 0.0123 0.0157 0.033 0.0109 0.0095 0.0365 0.0069 0.0124 0.0216 0.0252 0.0217
Endosome 0.0613 0.0196 0.0425 0.105 0.0399 0.034 0.0507 0.0099 0.0249 0.0857 0.0685 0.0374 0.043 0.0127 0.0374 0.0522 0.0262 0.0265
Golgi 0.0194 0.0024 0.0204 0.0054 0.0069 0.004 0.0083 0.0018 0.0075 0.0282 0.0253 0.0115 0.0074 0.0016 0.0047 0.0072 0.0043 0.0032
Lipid Particles 0.0128 0.0002 0.0051 0.0064 0.0016 0.0004 0.0053 0.0007 0.0015 0.0199 0.0132 0.0005 0.0052 0.0001 0.0013 0.0009 0.0008 0.0017
Mitochondria 0.0069 0.001 0.0225 0.0011 0.0057 0.0015 0.0055 0.0004 0.0069 0.0189 0.0047 0.0104 0.0031 0.0004 0.003 0.001 0.0537 0.002
None 0.0332 0.0275 0.024 0.0038 0.0086 0.0231 0.062 0.029 0.0271 0.02 0.0061 0.0153 0.0313 0.004 0.0166 0.0088 0.0069 0.0045
Nuclear Periphery 0.0052 0.0006 0.001 0.0014 0.0023 0.0104 0.0116 0.0008 0.0011 0.0075 0.0341 0.0009 0.0074 0.0008 0.0025 0.0017 0.0013 0.0046
Nucleolus 0.0002 0.0001 0.001 0.0001 0.0001 0.0001 0.001 0 0 0.0002 0.0002 0 0.0013 0 0.001 0 0.0001 0.0001
Nucleus 0.0017 0.0008 0.0016 0.001 0.0029 0.0019 0.0059 0.001 0.0008 0.0018 0.0395 0.0009 0.0029 0.0007 0.0037 0.0012 0.0011 0.0011
Peroxisomes 0.0084 0.0001 0.0048 0.0143 0.0007 0.0003 0.0011 0.0033 0.0083 0.0395 0.008 0.0003 0.0045 0.0001 0.0008 0.0002 0.0023 0.0006
Punctate Nuclear 0.0019 0.0002 0.006 0.0019 0.0009 0.0003 0.0048 0.0002 0.0004 0.0043 0.0069 0.0003 0.0023 0.0001 0.0062 0.0004 0.0004 0.0007
Vacuole 0.0051 0.0027 0.0055 0.0101 0.0071 0.002 0.0076 0.0027 0.003 0.0076 0.008 0.0018 0.0066 0.0017 0.0053 0.0056 0.0016 0.0013
Vacuole Periphery 0.0021 0.0005 0.0012 0.0014 0.0023 0.0014 0.0017 0.0003 0.0007 0.0028 0.0074 0.0008 0.0015 0.0004 0.0012 0.0013 0.0006 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.1803 20.2334 23.6471 31.8745 22.3762 15.835 30.9426 24.1635 23.4244 33.9641
Translational Efficiency 1.1084 1.4257 1.0962 0.7381 1.0946 1.9118 0.9536 1.4325 1.4098 0.8981

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (89%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Whi4

Whi4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Whi4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available