Standard name
Human Ortholog
Description Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.95 0.89 0.93 0.86 0.74 0.69 0.65 0.66 0.61 0.6 0.95 0.96 0.93 0.92 0.91 0.92 0.95 0.94 0.86 0.84 0.89 0.94
Endoplasmic Reticulum 0 0 0 0.08 0 0.08 0.19 0.2 0.24 0.15 0.2 0.22 0.05 0 0 0 0 0.05 0 0 0.05 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.06 0.09 0.11 0.15 0.14 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.06 0.07 0.05 0 0 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 6 3 11 8 6 11 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 2 1 1 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 5 4 3 3 4 5 6 6 3 4 16 1 4 2 2 5 2 0 0 0 0 0 0
Cytoplasm 475 271 144 135 156 113 141 117 129 84 80 98 40 125 119 171 177 143 477 260 137 51 102 86
Endoplasmic Reticulum 8 7 4 12 6 10 37 34 47 19 26 36 2 5 5 5 6 7 1 5 8 1 2 0
Endosome 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0
Golgi 0 0 3 3 2 4 11 16 22 19 19 16 1 0 2 6 5 5 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 0 2 4 3 1 13 6 0 0 0 1 0 1 0 0 1 0 1 0
Nucleus 2 0 0 0 1 0 1 0 0 0 1 0 0 1 1 0 0 0 1 1 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0
Vac/Vac Membrane 5 1 6 2 1 2 1 1 3 7 3 2 0 0 3 11 13 7 3 5 4 3 1 1
Unique Cell Count 488 277 152 151 167 132 191 170 198 127 132 162 42 130 128 185 194 155 501 278 159 61 115 92
Labelled Cell Count 495 284 161 156 170 134 204 181 221 141 152 185 45 135 133 196 207 165 501 278 159 61 115 92


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 43.8 49.1 36.0 48.8 49.4 48.1 42.4 40.7 42.0 42.3 37.5 40.0 39.1 39.9 39.4 50.3 51.8 51.7 30.1 36.3 38.2
Std Deviation (1e-4) 11.2 9.6 8.9 14.5 13.8 11.7 10.8 11.5 9.9 10.3 10.4 8.6 17.1 14.8 15.6 16.0 16.0 18.1 13.7 15.1 13.1
Intensity Change (Log2) – – – 0.44 0.46 0.42 0.24 0.18 0.22 0.24 0.06 0.15 0.12 0.15 0.13 0.48 0.53 0.52 -0.26 0.01 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.3 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 2.6 0 0 0 0 0 0
Cytoplasm -1.7 -0.5 -2.6 -5.1 -5.9 -6.6 -6.2 -7.0 -7.2 0.1 0.6 -0.6 -0.9 -1.2 -0.9
Endoplasmic Reticulum 2.1 0 1.9 4.7 4.8 5.5 3.7 4.7 5.2 0 0 0 0 0 0.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 1.8 2.8 3.3 4.0 3.9 2.9 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 4.0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0.6 0 0 0 0 0 0.8 1.1 0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 77.0347 112.8061 97.6568 91.4034 76.9486 92.3646 66.0685 108.7505 94.3481 93.5055 84.2666 89.3767 85.845 130.847 110.5077 103.8563 110.158 92.7712
Actin 0.0023 0.0003 0.0018 0.029 0.0054 0.003 0.0058 0.0009 0.001 0.0003 0.0082 0.0005 0.0205 0.0013 0.0101 0.0007 0.0007 0.0162
Bud 0.0019 0.0008 0.0012 0.0013 0.0028 0.003 0.0031 0.001 0.0023 0.003 0.0037 0.0013 0.0027 0.0016 0.0025 0.002 0.0007 0.0031
Bud Neck 0.0049 0.0006 0.0024 0.0006 0.0015 0.0019 0.002 0.0005 0.0031 0.0008 0.0014 0.0018 0.0014 0.0004 0.0007 0.0006 0.0006 0.0028
Bud Periphery 0.003 0.0008 0.0012 0.0017 0.0045 0.0104 0.0056 0.0008 0.0057 0.0041 0.0041 0.0009 0.0033 0.001 0.0014 0.0018 0.0002 0.0046
Bud Site 0.0025 0.0005 0.001 0.0005 0.0008 0.0005 0.0027 0.0009 0.0029 0.0007 0.0055 0.0002 0.0024 0.001 0.0012 0.0006 0.0001 0.0014
Cell Periphery 0.0202 0.0134 0.0273 0.0203 0.0072 0.0168 0.0209 0.0137 0.0201 0.0095 0.0053 0.0036 0.0255 0.0109 0.0075 0.0217 0.0015 0.03
Cytoplasm 0.5934 0.8745 0.6856 0.5644 0.4876 0.541 0.6025 0.8702 0.7584 0.5454 0.5505 0.7669 0.569 0.872 0.7183 0.4537 0.7774 0.4811
Cytoplasmic Foci 0.0156 0.0016 0.0091 0.0066 0.0382 0.01 0.0191 0.0018 0.0126 0.004 0.0337 0.0077 0.0232 0.0024 0.0139 0.0069 0.0164 0.0216
Eisosomes 0.0004 0 0.0001 0.0013 0.0002 0.0002 0.001 0.0001 0.0001 0.0001 0.0005 0 0.0014 0.0003 0.0004 0.0032 0 0.0025
Endoplasmic Reticulum 0.0361 0.0111 0.0195 0.0037 0.0111 0.1216 0.037 0.0147 0.0216 0.004 0.0171 0.0357 0.023 0.0039 0.0113 0.0027 0.0034 0.0189
Endosome 0.0196 0.0045 0.0145 0.0263 0.0497 0.0639 0.0203 0.0049 0.0167 0.0343 0.0377 0.0222 0.0269 0.0062 0.025 0.022 0.043 0.1347
Golgi 0.0037 0.002 0.0033 0.0068 0.01 0.0203 0.0059 0.0009 0.0032 0.004 0.0073 0.0029 0.007 0.004 0.0036 0.0015 0.0108 0.0448
Lipid Particles 0.0065 0.001 0.0033 0.0132 0.0111 0.0011 0.0085 0.0014 0.0049 0.0054 0.0165 0.001 0.0171 0.0006 0.0049 0.006 0.0031 0.0093
Mitochondria 0.0222 0.0013 0.0063 0.0446 0.0226 0.0155 0.0215 0.0047 0.0097 0.0149 0.0335 0.0067 0.029 0.0033 0.0237 0.0317 0.0009 0.0346
None 0.0039 0.0002 0.0018 0.003 0.0086 0.0046 0.0119 0.0003 0.0032 0.0006 0.0293 0.0012 0.0074 0.0004 0.0056 0.0165 0.0006 0.0037
Nuclear Periphery 0.0168 0.0028 0.0143 0.0052 0.0286 0.0057 0.0198 0.0022 0.0049 0.0071 0.0177 0.0061 0.0196 0.0018 0.005 0.0084 0.004 0.0309
Nucleolus 0.0067 0.0001 0.0037 0.0182 0.0254 0.0025 0.0168 0.0039 0.0032 0.0061 0.0069 0.0078 0.0039 0.0005 0.0059 0.0041 0.0005 0.0154
Nucleus 0.0076 0.0015 0.0037 0.0067 0.0153 0.0037 0.0136 0.0017 0.004 0.0076 0.0093 0.0066 0.0085 0.0018 0.004 0.0053 0.0023 0.0084
Peroxisomes 0.0008 0 0.0003 0.0034 0.001 0.0001 0.0021 0.0001 0.0015 0.0005 0.004 0.0002 0.0082 0.0001 0.0078 0.0004 0.0002 0.0031
Punctate Nuclear 0.0011 0 0.0055 0.001 0.0094 0.0001 0.0062 0 0.0008 0.0001 0.0009 0.0001 0.0108 0 0.0009 0.0003 0.0001 0.0027
Vacuole 0.1714 0.0749 0.1602 0.1826 0.1662 0.1135 0.1389 0.0658 0.1012 0.2662 0.1436 0.0795 0.1386 0.0715 0.1059 0.2962 0.0836 0.0572
Vacuole Periphery 0.0592 0.0078 0.0337 0.0595 0.093 0.0605 0.0349 0.0092 0.0187 0.0813 0.0633 0.0471 0.0507 0.0149 0.0405 0.1137 0.05 0.073

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3125.7136 3604.3963 4761.9399 3121.9047 3909.3301 3586.1746 3152.372 2765.9577 3369.6469 2489.4613
Translational Efficiency 0.813 0.8083 0.6354 1.0649 0.6725 0.6113 0.7719 0.7701 0.6166 0.7891

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Ssb1

Ssb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available