Standard name | |
---|---|
Human Ortholog | |
Description | Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 4 |
Bud | 0 | 0 | 3 | 1 | 0 | 2 | 2 | 1 | 4 | 4 | 6 | 8 | 0 | 0 | 0 | – | 0 | 0 | 3 | 1 | 2 | 3 | 8 | 16 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 6 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 2 | 2 | 7 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 65 | 89 | 67 | 61 | 92 | 98 | 155 | 144 | 70 | 143 | 77 | 145 | 142 | 251 | 368 | – | 117 | 83 | 53 | 72 | 48 | 95 | 182 | 119 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | – | 10 | 8 | 3 | 0 | 1 | 0 | 3 | 5 |
Endosome | 0 | 0 | 0 | 2 | 7 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 6 | 1 | 16 | – | 5 | 6 | 3 | 2 | 2 | 1 | 2 | 5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 4 |
Mitochondria | 37 | 30 | 37 | 56 | 56 | 143 | 242 | 223 | 170 | 265 | 209 | 341 | 17 | 20 | 12 | – | 9 | 9 | 19 | 7 | 14 | 12 | 18 | 24 |
Nucleus | 6 | 17 | 12 | 12 | 19 | 27 | 25 | 39 | 17 | 27 | 21 | 34 | 44 | 42 | 20 | – | 48 | 39 | 20 | 43 | 27 | 49 | 42 | 19 |
Nuclear Periphery | 1 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 0 | 1 | 3 | 0 | 1 | 0 | – | 4 | 2 | 1 | 1 | 2 | 11 | 12 | 7 |
Vac/Vac Membrane | 25 | 64 | 18 | 16 | 54 | 27 | 22 | 29 | 16 | 17 | 15 | 26 | 97 | 164 | 200 | – | 77 | 45 | 43 | 63 | 40 | 55 | 66 | 104 |
Unique Cell Count | 109 | 167 | 111 | 127 | 191 | 241 | 365 | 349 | 221 | 353 | 253 | 434 | 275 | 437 | 551 | 231 | 162 | 163 | 199 | 149 | 242 | 357 | 328 | |
Labelled Cell Count | 136 | 202 | 138 | 150 | 229 | 301 | 454 | 444 | 284 | 460 | 332 | 565 | 306 | 480 | 617 | 272 | 193 | 163 | 199 | 149 | 242 | 357 | 328 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 3.9 | 3.8 | 3.7 | 3.9 | 3.2 | 2.6 | 3.2 | 2.9 | 2.6 | 2.7 | 2.6 | 4.4 | 4.2 | 4.2 | 4.1 | 5.1 | 5.4 | 4.2 | 4.4 | 5.0 |
Std Deviation (1e-4) | 0.9 | 0.9 | 1.6 | 1.3 | 2.0 | 1.3 | 1.0 | 1.5 | 1.2 | 1.4 | 0.9 | 1.3 | 1.3 | 1.0 | 1.2 | 1.0 | 1.1 | 1.0 | 1.5 | 1.5 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.05 | -0.22 | -0.54 | -0.26 | -0.37 | -0.52 | -0.46 | -0.51 | 0.23 | 0.15 | 0.15 | 0.12 | 0.43 | 0.52 | 0.16 | 0.23 | 0.4 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 3.3 | 2.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.1804 | 0.008 | 0.0456 | -0.0808 | -0.1964 | -0.1834 | -0.0493 | 0.2583 | 0.1822 | -0.381 | 0.2768 | -0.025 | 1.6932 | 1.9835 | 1.9881 | 1.9357 | 1.6577 | 1.6286 |
Actin | 0.0189 | 0.0008 | 0.0137 | 0.053 | 0.0213 | 0.0082 | 0.0159 | 0.0004 | 0.0092 | 0.0004 | 0.0571 | 0.0033 | 0.0289 | 0.0008 | 0.0087 | 0.0025 | 0.0087 | 0.0007 |
Bud | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0013 | 0.0006 | 0 | 0.0001 | 0 | 0.0004 | 0.0003 | 0.0073 | 0.0026 | 0.0038 | 0.0059 | 0.0026 | 0.0016 |
Bud Neck | 0.0054 | 0.0007 | 0.0037 | 0.0011 | 0.0004 | 0.0067 | 0.0034 | 0.0003 | 0.0004 | 0.0005 | 0.0007 | 0.0033 | 0.0061 | 0.0007 | 0.0006 | 0.0013 | 0.0007 | 0.0072 |
Bud Periphery | 0.0011 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0005 | 0.0011 | 0 | 0.0002 | 0 | 0.0006 | 0.0007 | 0.0022 | 0.0006 | 0.0015 | 0.0056 | 0.0108 | 0.0022 |
Bud Site | 0.0032 | 0.003 | 0.0011 | 0.0006 | 0.0002 | 0.0003 | 0.0046 | 0.0005 | 0.001 | 0.0001 | 0.0009 | 0.0004 | 0.0037 | 0.0021 | 0.0057 | 0.0025 | 0.0016 | 0.0005 |
Cell Periphery | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0002 |
Cytoplasm | 0.1799 | 0.1893 | 0.1424 | 0.1127 | 0.1236 | 0.126 | 0.141 | 0.1983 | 0.1344 | 0.0846 | 0.1878 | 0.135 | 0.1684 | 0.1725 | 0.1325 | 0.0941 | 0.1302 | 0.1843 |
Cytoplasmic Foci | 0.0129 | 0.0078 | 0.0124 | 0.0029 | 0.0138 | 0.0061 | 0.0361 | 0.004 | 0.0121 | 0.0024 | 0.0108 | 0.0053 | 0.0111 | 0.0031 | 0.0097 | 0.0123 | 0.0051 | 0.0061 |
Eisosomes | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0018 | 0.0002 | 0.0004 | 0.0005 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0077 | 0.0072 | 0.0035 | 0.002 | 0.0015 | 0.0038 | 0.011 | 0.0057 | 0.0045 | 0.0017 | 0.0018 | 0.0022 | 0.0041 | 0.0029 | 0.0063 | 0.0013 | 0.0026 | 0.0025 |
Endosome | 0.0157 | 0.0036 | 0.008 | 0.0032 | 0.0042 | 0.0058 | 0.0361 | 0.0037 | 0.0116 | 0.0024 | 0.005 | 0.0033 | 0.0076 | 0.003 | 0.0069 | 0.0017 | 0.004 | 0.0036 |
Golgi | 0.0036 | 0.0002 | 0.003 | 0.0019 | 0.0022 | 0.0027 | 0.016 | 0.0001 | 0.0028 | 0.0001 | 0.0049 | 0.0035 | 0.0035 | 0.0001 | 0.0016 | 0.0006 | 0.0009 | 0.0001 |
Lipid Particles | 0.0157 | 0.002 | 0.0043 | 0.0008 | 0.0063 | 0.005 | 0.0291 | 0.0005 | 0.0036 | 0.0004 | 0.0404 | 0.0011 | 0.0097 | 0.0003 | 0.003 | 0.0065 | 0.0007 | 0.0005 |
Mitochondria | 0.0056 | 0.0006 | 0.0026 | 0.0012 | 0.001 | 0.0013 | 0.0131 | 0.0005 | 0.0013 | 0.0013 | 0.0044 | 0.0028 | 0.0046 | 0.001 | 0.003 | 0.0127 | 0.0113 | 0.0014 |
None | 0.3712 | 0.338 | 0.3719 | 0.2374 | 0.4843 | 0.244 | 0.473 | 0.5587 | 0.4789 | 0.3801 | 0.5373 | 0.4691 | 0.3957 | 0.4155 | 0.4133 | 0.3741 | 0.4461 | 0.3148 |
Nuclear Periphery | 0.0249 | 0.0235 | 0.0147 | 0.0123 | 0.0081 | 0.0088 | 0.0176 | 0.0119 | 0.0096 | 0.0173 | 0.0041 | 0.0065 | 0.0162 | 0.0093 | 0.0125 | 0.0098 | 0.0183 | 0.0104 |
Nucleolus | 0.0055 | 0.0041 | 0.0037 | 0.0047 | 0.0021 | 0.0094 | 0.0043 | 0.002 | 0.002 | 0.0063 | 0.0012 | 0.0054 | 0.0044 | 0.0033 | 0.0025 | 0.0082 | 0.0032 | 0.0069 |
Nucleus | 0.2742 | 0.3567 | 0.388 | 0.5332 | 0.3041 | 0.5076 | 0.1359 | 0.1965 | 0.3073 | 0.4233 | 0.1134 | 0.3357 | 0.2858 | 0.3628 | 0.3587 | 0.3697 | 0.309 | 0.4184 |
Peroxisomes | 0.0125 | 0.0004 | 0.0072 | 0.0004 | 0.015 | 0.0023 | 0.0233 | 0.0001 | 0.006 | 0.0001 | 0.0198 | 0.001 | 0.0144 | 0.0002 | 0.0032 | 0.0134 | 0.0005 | 0.0004 |
Punctate Nuclear | 0.0347 | 0.059 | 0.0171 | 0.0296 | 0.0104 | 0.0573 | 0.0276 | 0.0139 | 0.013 | 0.0775 | 0.0072 | 0.0196 | 0.021 | 0.0167 | 0.0236 | 0.0751 | 0.0416 | 0.0351 |
Vacuole | 0.0044 | 0.0021 | 0.0017 | 0.0017 | 0.0007 | 0.0023 | 0.0067 | 0.0021 | 0.0015 | 0.0008 | 0.0012 | 0.0012 | 0.0023 | 0.0019 | 0.0017 | 0.0013 | 0.0013 | 0.0026 |
Vacuole Periphery | 0.001 | 0.0004 | 0.0005 | 0.0003 | 0.0002 | 0.0004 | 0.0025 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0007 | 0.0003 | 0.0004 | 0.0005 | 0.0006 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.3827 | 3.5661 | 8.4678 | 10.3217 | 8.2754 | 8.7713 | 7.2113 | 5.5972 | 9.2732 | 10.8163 |
Translational Efficiency | 0.7285 | 0.7498 | 0.435 | 0.3773 | 0.443 | 0.5785 | 0.5853 | 0.6318 | 0.4732 | 0.4874 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|