Standard name
Human Ortholog
Description Highly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; regulated by phosphorylation mediated by TORC1, protein kinase A, Sch9p, casein kinase 2; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.05 0 0 0 0 0 0.06 0.11 0.15 0.19 0.2 0.25 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0.08 0.12 0.05 0.16 0.16 0.16 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.84 0.89 0.95 0.97 0.95 0.9 0.85 0.9 0.82 0.83 0.82 0.82 0.94 0.87 0.81 0.53 0.45 0.44 0.82 0.86 0.89 0.81 0.79 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0.11 0.12 0.17 0.18 0.23 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.16 0 0 0 0 0 0 0 0 0 0 0 0 0 0.35 0.39 0.35 0.05 0.08 0 0.07 0.08 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1
Bud 0 0 0 0 0 0 3 1 2 2 1 2 0 0 0 0 0 0 7 1 3 4 11 17
Bud Neck 0 0 0 0 0 0 0 2 2 0 1 2 0 0 0 0 0 0 0 0 0 0 2 2
Bud Site 0 0 0 0 1 3 6 12 10 15 20 23 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 2 6 7 12 10 25 10 7 9 8 13 20 56 78 25 36 26 7 2 2 9 12 11
Endoplasmic Reticulum 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 7 11 8 0 0 1 0 0 2
Endosome 7 0 1 0 0 1 1 1 0 1 0 0 0 2 2 3 2 5 4 0 1 0 9 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 2 0 0 0 2 0
Mitochondria 24 4 1 4 2 29 66 26 66 80 97 82 0 0 0 3 7 3 6 0 2 0 7 7
Nucleus 265 103 271 235 336 326 466 456 343 417 504 701 339 444 409 68 81 47 283 108 264 176 441 358
Nuclear Periphery 5 0 2 2 2 1 10 11 3 4 3 14 0 0 0 0 0 1 0 0 0 0 0 2
Nucleolus 4 0 3 2 4 20 61 62 70 91 138 193 0 4 4 1 0 1 4 1 2 0 10 15
Peroxisomes 0 0 0 0 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 1 1 2 1 2 6 0 0 0 5 0 4 4 11 12
Vac/Vac Membrane 26 18 5 0 2 1 1 1 1 1 2 2 5 2 8 45 70 37 17 9 10 14 43 72
Unique Cell Count 316 116 286 242 355 363 547 504 419 504 613 858 362 508 504 129 179 106 344 126 298 217 557 513
Labelled Cell Count 341 127 290 250 360 391 641 582 504 621 775 1034 365 512 509 153 209 128 344 126 298 217 557 513


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 6.6 6.6 6.2 6.7 5.9 5.8 5.9 5.6 5.4 5.3 5.6 7.9 7.9 7.5 8.9 8.6 9.9 7.2 7.3 7.9
Std Deviation (1e-4) 0.8 1.2 1.5 1.5 1.7 1.3 1.5 1.5 1.2 1.2 1.2 1.2 1.3 1.7 1.7 1.9 2.1 16.2 1.0 1.5 1.6
Intensity Change (Log2) – – – -0.11 0.02 -0.18 -0.19 -0.17 -0.26 -0.3 -0.32 -0.24 0.24 0.26 0.18 0.42 0.37 0.57 0.11 0.15 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2.9 3.1 2.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 1.0 0.5 1.8 -0.1 -0.4 -0.3 0 0 2.2 4.5 5.9 6.2 6.6 7.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.6 5.9 3.6 6.8 6.9 6.9 5.2 0 0 0 0 0 0
Nucleus 1.3 -0.1 -2.3 -4.1 -2.1 -5.0 -4.8 -5.1 -5.3 -0.6 -3.3 -5.3 -10.2 -12.1 -11.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.1 5.2 5.5 6.7 7.1 8.2 8.3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 9.6 10.7 9.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5021 3.5295 3.1633 3.0757 2.7628 3.1076 2.3644 2.8514 2.2194 2.296 2.2443 2.3399 2.0357 2.192 1.6561 1.8468 1.5036 1.4524
Actin 0.0201 0.0002 0 0 0.0001 0.0001 0.0631 0 0.0006 0.0038 0.0001 0.0007 0.0381 0.0001 0.0033 0.0114 0.0183 0.0001
Bud 0.0001 0.0001 0 0 0 0 0.0015 0 0.0004 0.0011 0.0001 0.0004 0.0008 0.0001 0.0001 0.0014 0.0001 0.0002
Bud Neck 0.0004 0.0005 0.0001 0.0001 0.0003 0.0024 0.0107 0.0001 0.0001 0.0005 0.0006 0.0015 0.0078 0.0001 0.0018 0.0023 0.0007 0.0012
Bud Periphery 0.0001 0 0 0 0 0.0001 0.0024 0 0.0004 0.0036 0.0001 0.0006 0.0019 0.0001 0.0001 0.0014 0.0002 0.0001
Bud Site 0.0001 0.0007 0.0001 0 0.0001 0.0001 0.0257 0.0001 0.0016 0.0011 0.0001 0.0011 0.0035 0.0001 0.0055 0.0141 0.0002 0.0004
Cell Periphery 0 0 0 0 0 0 0.0011 0 0 0.0002 0 0.0001 0.0004 0 0.0001 0.0003 0 0
Cytoplasm 0.0049 0.0949 0.0344 0.0259 0.0247 0.012 0.007 0.0359 0.0122 0.0125 0.0027 0.0085 0.0026 0.0142 0.0052 0.002 0.0033 0.0051
Cytoplasmic Foci 0.0008 0.0009 0.0002 0.0001 0.0003 0.0003 0.0451 0.0001 0.0026 0.0007 0 0.0023 0.0273 0.0001 0.0013 0.0032 0.0033 0.0026
Eisosomes 0.0002 0 0 0 0 0 0.0006 0 0 0.0001 0 0 0.0003 0 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0003 0.0007 0.001 0.0004 0.0016 0.0003 0.0086 0.0005 0.0002 0.0007 0.0003 0.0005 0.0041 0.0004 0.0002 0.0011 0.0005 0.0001
Endosome 0.0012 0.0004 0.0002 0.0005 0.0019 0.0004 0.0664 0.0002 0.0029 0.0106 0.0001 0.0069 0.0218 0.0002 0.0011 0.0082 0.0049 0.0021
Golgi 0.0011 0 0 0 0 0 0.0186 0 0.0023 0.0052 0 0.0061 0.0105 0 0.0013 0.0044 0.0074 0.0006
Lipid Particles 0.0008 0 0 0 0.0001 0 0.0199 0 0.0027 0.0022 0 0.0115 0.0167 0 0.0007 0.0075 0.0129 0.0003
Mitochondria 0.0003 0.0002 0.0001 0.0003 0.0003 0.0001 0.0127 0.0002 0.0026 0.0073 0.0006 0.0043 0.013 0.001 0.0015 0.0073 0.0013 0.0006
None 0.0166 0.0165 0.0124 0.0017 0.0149 0.0068 0.0041 0.0021 0.0007 0.0003 0.0002 0.0003 0.0019 0.0001 0.0007 0.0003 0.0004 0.0001
Nuclear Periphery 0.0027 0.0042 0.0049 0.0032 0.0291 0.0033 0.0204 0.0043 0.0045 0.0042 0.0099 0.0091 0.0188 0.0048 0.0013 0.0033 0.0029 0.0012
Nucleolus 0.0048 0.004 0.0033 0.0018 0.0069 0.0103 0.0113 0.0026 0.0025 0.0025 0.0091 0.0071 0.0046 0.0031 0.0025 0.0033 0.0086 0.0065
Nucleus 0.929 0.8644 0.9389 0.9634 0.905 0.9504 0.6142 0.9504 0.9557 0.9355 0.974 0.9321 0.7953 0.9675 0.9705 0.923 0.929 0.976
Peroxisomes 0.0004 0 0 0 0 0 0.0239 0 0.0044 0.0004 0 0.0016 0.0138 0 0.0002 0.0017 0.0034 0.0008
Punctate Nuclear 0.0159 0.0117 0.0038 0.0022 0.0135 0.0128 0.0114 0.0028 0.0029 0.0022 0.001 0.0033 0.0107 0.0066 0.0021 0.0015 0.0021 0.0017
Vacuole 0.0001 0.0005 0.0003 0.0003 0.0007 0.0004 0.0209 0.0003 0.0005 0.0033 0.0006 0.0012 0.0041 0.0007 0.0002 0.0015 0.0002 0.0003
Vacuole Periphery 0.0001 0.0001 0.0001 0.0002 0.0004 0.0001 0.0105 0.0001 0.0002 0.0021 0.0003 0.0007 0.0022 0.0006 0.0001 0.0006 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.0402 23.2479 12.9382 18.3993 19.2675 14.8524 17.6295 23.5141 20.4077 23.702
Translational Efficiency 0.9579 0.7359 1.1812 0.8098 0.8919 1.6515 1.3839 0.9421 1.0256 1.0396

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Highly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; regulated by phosphorylation mediated by TORC1, protein kinase A, Sch9p, casein kinase 2; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Maf1

Maf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Maf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available