Standard name
Human Ortholog
Description Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.08 0.13 0.2 0.36 0.27 0.43 0.38 0.45 0.41 0.36 0.41 0.4 0.2 0.13 0.13 0.12 0.12 0.33 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.08 0.05 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.88 0.91 0.9 0.93 0.84 0.68 0.65 0.51 0.65 0.54 0.69 0.6 0.94 0.95 0.94 0.79 0.73 0.86 0.92 0.93 0.95 0.94 0.92 0.93
Cytoplasm 0.08 0.05 0.11 0.07 0.13 0.15 0.15 0.17 0.19 0.18 0.18 0.14 0.06 0 0.06 0.18 0.24 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.11 0.13 0.1 0.36 0.35 0.43 0.41 0.45 0.38 0.5 0 0 0 0.07 0.05 0.17 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 45 42 46 65 52 119 115 138 107 112 115 140 39 30 27 12 11 12 3 5 0 5 4 7
Bud 6 1 1 3 7 10 16 26 14 22 22 13 2 1 2 2 3 0 6 3 1 1 2 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 2 0 1 0 2
Bud Site 0 0 0 0 0 1 2 1 1 4 0 0 1 0 0 1 0 0 – – – – – –
Cell Periphery 492 287 203 166 164 189 198 158 168 169 194 211 180 217 200 77 69 31 588 356 223 396 302 356
Cytoplasm 43 15 25 13 25 42 47 53 50 55 50 49 11 8 13 17 23 3 12 2 1 0 0 0
Endoplasmic Reticulum 27 11 1 1 4 7 10 5 8 8 7 11 2 4 2 8 5 0 13 6 3 5 8 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 8 14 25 23 20 100 108 132 107 139 105 173 3 0 2 7 5 6 3 2 0 8 4 3
Nucleus 2 1 0 0 1 0 0 1 0 2 0 2 1 1 1 0 4 0 0 0 0 0 0 0
Nuclear Periphery 2 0 0 0 0 5 1 3 5 1 1 3 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 2 9 5 6 4 2 0 8 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 2 0 3 0 1 0 2 1 0 2 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 1 0 1 0 1 0 1 0 0 0 0 1 0 2 2 0 0 1 0
Unique Cell Count 556 314 226 179 196 278 305 307 260 311 280 349 192 229 213 97 94 36 640 381 235 423 328 383
Labelled Cell Count 626 372 302 271 275 485 502 527 464 517 494 613 240 261 249 124 121 52 640 381 235 423 328 383


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.1 20.5 15.1 14.9 13.8 12.1 12.1 10.8 11.1 10.9 10.7 10.6 16.5 17.3 18.0 12.3 13.6 16.0 16.3 17.1 18.6
Std Deviation (1e-4) 3.8 3.9 2.9 3.1 3.4 3.2 3.1 2.7 3.0 2.8 2.7 2.8 3.3 3.9 5.2 3.0 3.7 3.5 3.2 3.4 3.5
Intensity Change (Log2) – – – -0.02 -0.13 -0.32 -0.32 -0.48 -0.45 -0.48 -0.5 -0.51 0.13 0.19 0.25 -0.3 -0.15 0.08 0.11 0.18 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.6 1.5 5.3 4.3 5.9 4.9 3.9 5.0 4.9 0 -2.1 -2.2 -1.7 -1.8 1.7
Bud 0 0 0 3.1 4.2 3.1 3.7 4.0 2.5 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.0 -1.9 -5.9 -6.6 -9.4 -6.5 -8.8 -5.6 -7.7 1.4 2.0 1.6 -2.5 -3.7 -0.7
Cytoplasm -1.3 0.5 1.3 1.4 2.0 2.5 2.1 2.1 1.0 -1.9 -3.1 -1.8 1.6 3.1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.6 -0.3 6.4 6.4 8.0 7.4 8.4 6.8 9.5 -3.9 -5.2 -4.4 -1.1 -1.6 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0379 0.0002 0.0057 0.0286 0.0282 0.0016 0.015 0 0.0129 0 0.0006 0.0002 0.0969 0.0032 0 0 0 0.0004
Bud 0.002 0.0002 0.0014 0.0005 0.0015 0.0002 0.0017 0.0001 0.0002 0.0001 0.0012 0.0002 0.0073 0.0004 0.0001 0 0.0001 0.0005
Bud Neck 0.0014 0.0007 0.0029 0.0078 0.0011 0.0005 0.0012 0.0002 0.0004 0.0003 0.001 0.0001 0.0067 0.0009 0.0019 0.0012 0.0008 0.0009
Bud Periphery 0.0156 0.0013 0.0099 0.0049 0.0046 0.0058 0.0177 0.0008 0.0021 0.0024 0.015 0.0034 0.049 0.0027 0.0017 0.0015 0.0034 0.006
Bud Site 0.017 0.0293 0.0062 0.0057 0.0105 0.0004 0.0072 0.01 0.0024 0.0002 0.0026 0.0001 0.0153 0.0157 0.0066 0.0005 0.0012 0.0014
Cell Periphery 0.7263 0.8819 0.8709 0.818 0.8825 0.9385 0.7721 0.9508 0.9198 0.9528 0.944 0.9574 0.1738 0.872 0.9323 0.9507 0.9622 0.9574
Cytoplasm 0.0142 0.0436 0.0412 0.0446 0.0192 0.0131 0.0109 0.0176 0.0143 0.0048 0.0111 0.0037 0.0044 0.0346 0.0404 0.0135 0.0155 0.0117
Cytoplasmic Foci 0.0202 0 0.0025 0.006 0.003 0.0008 0.0172 0 0.0023 0 0 0.0001 0.0019 0.0001 0 0 0 0
Eisosomes 0.0006 0.0021 0.0079 0.0006 0.0002 0.0002 0.0104 0.0004 0.0005 0.0001 0.0004 0.0007 0.0752 0.038 0 0.0001 0.0045 0.0002
Endoplasmic Reticulum 0.0161 0.027 0.016 0.0392 0.0254 0.016 0.0173 0.0102 0.0171 0.0251 0.009 0.0122 0.0081 0.0129 0.0067 0.0216 0.005 0.0136
Endosome 0.0193 0 0.0048 0.0049 0.0023 0.0012 0.0115 0 0.0012 0 0.0003 0.0025 0.0099 0.0002 0 0 0 0
Golgi 0.0103 0 0.0012 0.0026 0.0029 0.0019 0.0027 0 0.001 0 0.0002 0.0001 0.0206 0.0008 0 0 0 0
Lipid Particles 0.0325 0.0004 0.0022 0.0044 0.0013 0.0053 0.0052 0.0002 0.0012 0.0002 0.0002 0.0003 0.0049 0.0006 0.0002 0.0002 0.0002 0.0003
Mitochondria 0.0086 0.0001 0.0043 0.0006 0.0016 0.0005 0.0191 0 0.0009 0 0.0009 0.0067 0.4924 0.0019 0 0 0.0001 0
None 0.0031 0.0001 0.0011 0.0034 0.0001 0.0001 0.0213 0 0.0046 0 0.0001 0.0001 0.0022 0.002 0 0 0.0001 0
Nuclear Periphery 0.0217 0.0018 0.0036 0.0043 0.0015 0.0011 0.0184 0.0012 0.0016 0.0015 0.0006 0.0024 0.0014 0.006 0.0009 0.0012 0.0014 0.001
Nucleolus 0.0014 0.0001 0.0002 0.0007 0.0001 0 0.0006 0 0.0002 0 0.0005 0 0.0015 0.0003 0 0 0 0
Nucleus 0.0037 0.0002 0.0006 0.0015 0.0003 0.0001 0.0076 0.0001 0.0005 0.0001 0.0006 0.0004 0.002 0.0006 0.0002 0.0001 0.0001 0.0001
Peroxisomes 0.0157 0 0.0011 0.0016 0.0016 0.0018 0.0038 0 0.0051 0 0 0 0.0019 0.0001 0 0 0 0
Punctate Nuclear 0.0039 0 0.0031 0.0106 0.0005 0.0004 0.0055 0 0.001 0 0 0.0001 0.0001 0 0 0 0 0
Vacuole 0.0266 0.0107 0.0127 0.0092 0.0115 0.0104 0.0304 0.0083 0.0105 0.0123 0.0112 0.0091 0.0176 0.0066 0.0088 0.0094 0.0052 0.0064
Vacuole Periphery 0.0019 0.0001 0.0004 0.0002 0.0002 0.0001 0.0032 0 0.0002 0.0001 0.0005 0.0002 0.0068 0.0003 0.0001 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 92.685 200.2961 170.7196 170.7735 111.1217 113.5589 233.0077 223.4045 188.1597 134.3014
Translational Efficiency 0.6605 0.754 0.6681 0.6148 0.5774 0.6082 0.5922 0.4687 0.5016 0.4806

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
Localization
Cell Percentages cell periphery (97%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-1

Snq2

Snq2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snq2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available