Standard name | |
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Human Ortholog | |
Description | Inositol hexakisphosphate and inositol heptakisphosphate kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response, RNA polymerase I-mediated rRNA transcription and telomere maintenance; inositol hexakisphosphate is also known as IP6; inositol heptakisphosphate is also known as IP7 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 |
Bud | 0 | 0 | 3 | 1 | 4 | 2 | 4 | 12 | 12 | 7 | 5 | 11 | 1 | 1 | 17 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 4 | 2 | 2 | 7 | 6 | 11 | 14 | 3 | 11 | 8 | 11 | 8 | 14 | 25 | 12 | 8 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 168 | 150 | 224 | 296 | 421 | 370 | 603 | 695 | 306 | 386 | 192 | 329 | 320 | 524 | 569 | 112 | 107 | 145 | 162 | 145 | 220 | 149 | 306 | 358 |
Endoplasmic Reticulum | 1 | 1 | 0 | 3 | 2 | 3 | 5 | 3 | 6 | 8 | 5 | 0 | 1 | 2 | 2 | 6 | 3 | 8 | 0 | 0 | 2 | 1 | 6 | 15 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 3 | 6 |
Golgi | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 3 |
Mitochondria | 1 | 0 | 7 | 25 | 14 | 63 | 137 | 208 | 172 | 185 | 120 | 233 | 0 | 2 | 22 | 6 | 4 | 6 | 1 | 0 | 5 | 2 | 2 | 3 |
Nucleus | 0 | 1 | 0 | 0 | 2 | 2 | 0 | 4 | 0 | 3 | 1 | 0 | 0 | 2 | 0 | 5 | 4 | 4 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 8 | 0 | 2 | 1 | 2 | 0 | 2 | 1 | 2 | 8 | 11 | 0 | 3 | 6 | 1 | 7 | 4 |
Unique Cell Count | 172 | 153 | 227 | 306 | 426 | 388 | 649 | 804 | 386 | 483 | 272 | 453 | 321 | 532 | 581 | 126 | 118 | 163 | 173 | 154 | 241 | 161 | 342 | 400 |
Labelled Cell Count | 175 | 156 | 236 | 328 | 451 | 446 | 764 | 948 | 500 | 607 | 334 | 587 | 330 | 551 | 640 | 146 | 136 | 180 | 173 | 154 | 241 | 161 | 342 | 400 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.7 | 6.9 | 5.2 | 5.1 | 5.3 | 4.7 | 4.5 | 4.5 | 4.0 | 3.9 | 4.0 | 3.8 | 7.7 | 7.5 | 6.3 | 9.4 | 10.2 | 10.3 | 7.7 | 7.2 | 8.2 |
Std Deviation (1e-4) | 1.0 | 1.0 | 0.6 | 0.7 | 0.9 | 0.6 | 0.8 | 0.8 | 0.7 | 0.7 | 0.6 | 0.6 | 0.9 | 1.2 | 1.0 | 1.6 | 1.7 | 1.8 | 1.1 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.02 | -0.16 | -0.22 | -0.21 | -0.39 | -0.41 | -0.4 | -0.46 | 0.56 | 0.53 | 0.26 | 0.85 | 0.97 | 0.98 | 0.56 | 0.46 | 0.66 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 |
Cytoplasm | -1.4 | 0.2 | -2.2 | -3.3 | -5.2 | -6.8 | -6.7 | -8.4 | -8.2 | 1.4 | -0.2 | -0.7 | -4.1 | -3.6 | -4.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.4 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0 | 0 | 0.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.9879 | 4.6943 | 4.205 | 4.0386 | 4.2173 | 4.4613 | 4.5916 | 6.0143 | 5.8379 | 5.5647 | 5.0937 | 5.2076 | 2.4342 | 3.5236 | 2.8031 | 3.1119 | 2.4783 | 2.8463 |
Actin | 0.0912 | 0.0005 | 0.0096 | 0.0004 | 0.0061 | 0.0018 | 0.0435 | 0.0015 | 0.0017 | 0.0142 | 0.0134 | 0.0031 | 0.0076 | 0.0016 | 0.0023 | 0.0001 | 0.0007 | 0.0012 |
Bud | 0.0026 | 0.001 | 0.0007 | 0.0004 | 0.0019 | 0.0004 | 0.0014 | 0.0004 | 0.0005 | 0.0006 | 0.0015 | 0.0002 | 0.0015 | 0.0007 | 0.0004 | 0.0003 | 0.0003 | 0.0001 |
Bud Neck | 0.0473 | 0.0006 | 0.0005 | 0.0033 | 0.0008 | 0.002 | 0.0016 | 0.0002 | 0.0007 | 0.0013 | 0.0012 | 0.0007 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0004 | 0.0004 |
Bud Periphery | 0.0031 | 0.0004 | 0.0006 | 0.0004 | 0.0067 | 0.0003 | 0.0012 | 0.0002 | 0.0004 | 0.0005 | 0.0021 | 0.0002 | 0.0039 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0189 | 0.0113 | 0.0019 | 0.0026 | 0.0049 | 0.0009 | 0.0045 | 0.0083 | 0.0032 | 0.0035 | 0.0026 | 0.0001 | 0.0022 | 0.0045 | 0.001 | 0.0002 | 0.0002 | 0.0001 |
Cell Periphery | 0.0014 | 0.0005 | 0.0006 | 0.0001 | 0.0014 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.5976 | 0.9384 | 0.8981 | 0.8572 | 0.8296 | 0.9518 | 0.7415 | 0.9466 | 0.9164 | 0.8818 | 0.7595 | 0.9344 | 0.7841 | 0.9417 | 0.9387 | 0.867 | 0.898 | 0.9273 |
Cytoplasmic Foci | 0.0416 | 0.0072 | 0.0164 | 0.0341 | 0.0076 | 0.0084 | 0.0443 | 0.0136 | 0.021 | 0.0187 | 0.0381 | 0.013 | 0.0333 | 0.0178 | 0.0112 | 0.0158 | 0.0204 | 0.0112 |
Eisosomes | 0.0006 | 0 | 0 | 0 | 0.0002 | 0 | 0.0007 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0112 | 0.0018 | 0.0066 | 0.0032 | 0.0044 | 0.0033 | 0.0086 | 0.0018 | 0.0062 | 0.0017 | 0.0026 | 0.0137 | 0.0081 | 0.0009 | 0.0037 | 0.0014 | 0.008 | 0.0026 |
Endosome | 0.0514 | 0.0053 | 0.0149 | 0.0324 | 0.0288 | 0.0082 | 0.0473 | 0.0051 | 0.0151 | 0.0195 | 0.0467 | 0.012 | 0.016 | 0.0045 | 0.0069 | 0.022 | 0.0391 | 0.0084 |
Golgi | 0.0204 | 0.0011 | 0.004 | 0.0046 | 0.015 | 0.0015 | 0.0099 | 0.0007 | 0.005 | 0.0055 | 0.0089 | 0.0028 | 0.0036 | 0.0008 | 0.001 | 0.0008 | 0.0022 | 0.0022 |
Lipid Particles | 0.0161 | 0.0002 | 0.0028 | 0.0004 | 0.0221 | 0.0005 | 0.0063 | 0.0004 | 0.0009 | 0.0026 | 0.0088 | 0.0014 | 0.0072 | 0.0002 | 0.0008 | 0 | 0.0004 | 0.0012 |
Mitochondria | 0.0069 | 0.0062 | 0.0015 | 0.0004 | 0.0372 | 0.009 | 0.0072 | 0.0008 | 0.0016 | 0.0013 | 0.0149 | 0.0078 | 0.0015 | 0.0002 | 0.0002 | 0.0004 | 0.0004 | 0.0111 |
None | 0.0285 | 0.017 | 0.0166 | 0.0432 | 0.0172 | 0.008 | 0.0495 | 0.0155 | 0.0123 | 0.0094 | 0.0031 | 0.0043 | 0.0411 | 0.0222 | 0.0166 | 0.084 | 0.0252 | 0.0276 |
Nuclear Periphery | 0.0216 | 0.0003 | 0.0028 | 0.001 | 0.0041 | 0.0003 | 0.0069 | 0.0003 | 0.0009 | 0.0027 | 0.0215 | 0.0023 | 0.0116 | 0.0002 | 0.0005 | 0.0004 | 0.0011 | 0.0002 |
Nucleolus | 0.0017 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0028 | 0 | 0.0004 | 0.0004 | 0.0014 | 0 | 0.0009 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0045 | 0.0038 | 0.0022 | 0.0009 | 0.0014 | 0.0006 | 0.0039 | 0.0005 | 0.0015 | 0.0019 | 0.0267 | 0.0011 | 0.0368 | 0.0005 | 0.0006 | 0.0006 | 0.0006 | 0.0003 |
Peroxisomes | 0.0089 | 0.001 | 0.0127 | 0.0025 | 0.0028 | 0.0007 | 0.0064 | 0.0004 | 0.0018 | 0.0016 | 0.008 | 0.0003 | 0.0185 | 0.0006 | 0.0129 | 0.0002 | 0.0002 | 0.0046 |
Punctate Nuclear | 0.0116 | 0.0002 | 0.0018 | 0.0003 | 0.0009 | 0.0004 | 0.0038 | 0.0017 | 0.0011 | 0.028 | 0.0252 | 0.0006 | 0.017 | 0.0006 | 0.0003 | 0.0001 | 0.0004 | 0.0001 |
Vacuole | 0.0099 | 0.0027 | 0.0046 | 0.011 | 0.0045 | 0.0015 | 0.0067 | 0.0014 | 0.0081 | 0.004 | 0.0095 | 0.0013 | 0.0033 | 0.0021 | 0.002 | 0.0049 | 0.0017 | 0.001 |
Vacuole Periphery | 0.003 | 0.0002 | 0.0008 | 0.0015 | 0.0021 | 0.0003 | 0.0018 | 0.0002 | 0.001 | 0.0009 | 0.0044 | 0.0007 | 0.0008 | 0.0002 | 0.0003 | 0.001 | 0.0005 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.0462 | 22.2115 | 18.339 | 22.6684 | 24.5014 | 22.3846 | 24.514 | 25.1915 | 22.5664 | 30.5127 |
Translational Efficiency | 0.8632 | 0.9475 | 0.9005 | 0.6105 | 0.7498 | 0.849 | 0.6851 | 0.5693 | 0.5324 | 0.6206 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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