Standard name | |
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Human Ortholog | |
Description | Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; N-terminally propionylated in vivo; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 9 | 8 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 7 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 3 | 2 | 1 | 2 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 3 | 2 | 0 | 0 | 1 | 3 | 3 | 2 | 2 | 2 | 1 | 0 | 2 | 4 | 8 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 157 | 211 | 290 | 221 | 199 | 151 | 207 | 216 | 284 | 309 | 190 | 213 | 350 | 445 | 479 | 417 | 217 | 229 | 203 | 208 | 264 | 312 | 399 | 436 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 2 | 3 | 1 | 0 | 0 | 24 | 11 | 3 | 4 | 0 | 1 | 1 | 1 | 2 |
Endosome | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 1 | 0 | 0 | 1 | 0 | 5 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 4 | 5 | 0 | 0 | 4 | 4 | 3 | 13 | 109 | 81 | 96 | 84 | 0 | 0 | 0 | 23 | 7 | 3 | 4 | 5 | 6 | 2 | 2 | 1 |
Nucleus | 12 | 0 | 0 | 1 | 3 | 1 | 0 | 2 | 2 | 2 | 3 | 5 | 0 | 0 | 1 | 1 | 1 | 5 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 4 |
Vac/Vac Membrane | 0 | 0 | 3 | 0 | 1 | 0 | 1 | 3 | 4 | 3 | 3 | 1 | 0 | 0 | 1 | 7 | 6 | 7 | 1 | 1 | 1 | 2 | 3 | 4 |
Unique Cell Count | 174 | 214 | 292 | 221 | 204 | 154 | 213 | 227 | 339 | 369 | 254 | 277 | 351 | 447 | 480 | 435 | 229 | 238 | 225 | 223 | 286 | 330 | 420 | 464 |
Labelled Cell Count | 182 | 219 | 299 | 224 | 211 | 162 | 216 | 242 | 416 | 415 | 304 | 315 | 352 | 448 | 484 | 481 | 252 | 252 | 225 | 223 | 286 | 330 | 420 | 464 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 3.9 | 5.6 | 5.4 | 4.6 | 3.9 | 4.5 | 4.6 | 3.7 | 4.3 | 4.3 | 4.1 | 5.9 | 5.8 | 5.0 | 5.5 | 7.8 | 7.5 | 6.4 | 6.6 | 6.6 |
Std Deviation (1e-4) | 0.6 | 0.5 | 1.0 | 0.9 | 1.5 | 0.9 | 1.4 | 1.4 | 1.0 | 1.4 | 1.4 | 0.9 | 1.4 | 1.2 | 1.0 | 1.2 | 1.7 | 1.6 | 1.6 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.28 | -0.52 | -0.32 | -0.3 | -0.61 | -0.39 | -0.37 | -0.46 | 0.08 | 0.04 | -0.16 | -0.03 | 0.48 | 0.42 | 0.2 | 0.25 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2084 | 4.0417 | 3.4505 | 3.6522 | 3.3148 | 3.5702 | 7.8588 | 8.8879 | 8.8794 | 7.9456 | 7.8187 | 8.7517 | 4.7849 | 5.268 | 4.7099 | 5.047 | 5.0877 | 5.1435 |
Actin | 0.0015 | 0.0002 | 0.0012 | 0.0017 | 0.0004 | 0.0005 | 0.0101 | 0.0003 | 0.0007 | 0.0009 | 0.0004 | 0.0008 | 0.0313 | 0.0039 | 0.0172 | 0.006 | 0.017 | 0.0079 |
Bud | 0.0002 | 0.0004 | 0.0004 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0009 | 0.0005 | 0.0005 | 0.0017 | 0.002 | 0.0002 | 0.0002 |
Bud Neck | 0.0003 | 0.0004 | 0.0006 | 0.0012 | 0.0007 | 0.0024 | 0.001 | 0.0008 | 0.0005 | 0.0005 | 0.0009 | 0.0011 | 0.0152 | 0.0016 | 0.0006 | 0.0017 | 0.001 | 0.0018 |
Bud Periphery | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0009 | 0.0006 | 0.0002 | 0.0019 | 0.0011 | 0.0002 | 0.0001 |
Bud Site | 0.0008 | 0.0016 | 0.0059 | 0.0008 | 0.0001 | 0.0001 | 0.0012 | 0.0074 | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0032 | 0.0038 | 0.0011 | 0.0123 | 0.0006 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 |
Cytoplasm | 0.8568 | 0.8911 | 0.8541 | 0.8312 | 0.8828 | 0.8926 | 0.8829 | 0.933 | 0.8879 | 0.9333 | 0.88 | 0.9073 | 0.6978 | 0.8502 | 0.849 | 0.9048 | 0.6626 | 0.8951 |
Cytoplasmic Foci | 0.0083 | 0.0065 | 0.01 | 0.0205 | 0.0259 | 0.0095 | 0.0121 | 0.006 | 0.0061 | 0.0035 | 0.0073 | 0.0049 | 0.0427 | 0.0157 | 0.0102 | 0.0121 | 0.0178 | 0.0101 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0009 | 0 | 0.0005 | 0.0001 |
Endoplasmic Reticulum | 0.0041 | 0.0015 | 0.0045 | 0.0033 | 0.0023 | 0.0025 | 0.0028 | 0.0013 | 0.0024 | 0.0025 | 0.0026 | 0.0014 | 0.0078 | 0.0021 | 0.0052 | 0.0062 | 0.0049 | 0.0036 |
Endosome | 0.0049 | 0.003 | 0.005 | 0.0065 | 0.0088 | 0.007 | 0.0057 | 0.0011 | 0.0043 | 0.0013 | 0.0022 | 0.0014 | 0.0321 | 0.0042 | 0.0112 | 0.0164 | 0.0313 | 0.0127 |
Golgi | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0006 | 0.0002 | 0.0015 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0105 | 0.0018 | 0.0018 | 0.0078 | 0.0063 | 0.0027 |
Lipid Particles | 0.0002 | 0 | 0.0002 | 0.0003 | 0.0009 | 0 | 0.0006 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0199 | 0.0011 | 0.0017 | 0.0002 | 0.0397 | 0.002 |
Mitochondria | 0.0003 | 0.0003 | 0.0006 | 0.0006 | 0.0003 | 0.0014 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0046 | 0.0005 | 0.0022 | 0.0008 | 0.0095 | 0.0014 |
None | 0.1019 | 0.0792 | 0.0903 | 0.0815 | 0.0598 | 0.0561 | 0.0625 | 0.0409 | 0.0753 | 0.0501 | 0.093 | 0.069 | 0.0877 | 0.0935 | 0.0622 | 0.0123 | 0.1363 | 0.0412 |
Nuclear Periphery | 0.003 | 0.0014 | 0.0029 | 0.0041 | 0.0024 | 0.0026 | 0.003 | 0.0006 | 0.002 | 0.0012 | 0.0018 | 0.0008 | 0.0106 | 0.0011 | 0.01 | 0.002 | 0.0082 | 0.0062 |
Nucleolus | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0009 | 0.0003 | 0.0004 | 0.0002 | 0.0011 | 0.0001 |
Nucleus | 0.0121 | 0.0117 | 0.0176 | 0.0325 | 0.0121 | 0.0197 | 0.0102 | 0.0058 | 0.0138 | 0.0043 | 0.0067 | 0.009 | 0.0064 | 0.0057 | 0.0112 | 0.0106 | 0.0259 | 0.0065 |
Peroxisomes | 0.0002 | 0.0002 | 0.0005 | 0.001 | 0.0003 | 0.0001 | 0.0013 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0185 | 0.0086 | 0.0027 | 0.0003 | 0.0071 | 0.0045 |
Punctate Nuclear | 0.0026 | 0.0008 | 0.0037 | 0.011 | 0.0014 | 0.0026 | 0.003 | 0.0011 | 0.0037 | 0.0008 | 0.0029 | 0.0015 | 0.0051 | 0.0039 | 0.0071 | 0.001 | 0.0269 | 0.0023 |
Vacuole | 0.0018 | 0.0012 | 0.0014 | 0.0016 | 0.0008 | 0.0016 | 0.0007 | 0.0004 | 0.0006 | 0.0005 | 0.0007 | 0.0003 | 0.0025 | 0.0009 | 0.001 | 0.0016 | 0.0017 | 0.0008 |
Vacuole Periphery | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0013 | 0.0001 | 0.0004 | 0.0003 | 0.001 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 1819.0946 | 1777.9902 | 1833.7192 | 2127.847 | 2024.4224 | 1503.1709 | 1808.8407 | 1668.5247 | 1573.6893 | 1804.0893 |
Translational Efficiency | 1.5851 | 1.7008 | 1.7592 | 1.0024 | 1.6503 | 2.0701 | 1.4444 | 1.5741 | 1.582 | 1.6193 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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