Standard name
Human Ortholog
Description RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.06 0.05 0.09 – 0.06 0.07 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0.05 0 0.07 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.05 0.06 0.06 0.07 – 0.05 0.06 0.05 0.06 0.08 0.17 0.23 0.08 0.06 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.09 0.13 0.09 0.06 0.05 0.05 0 – 0 0 0 0 0.05 0.09 0.19 0.29 0.31 0.35 0.11 0.09 0.12 0.08 0.11 0.15
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0.06 0 0 0 0.1 0 – 0.24 0.14 0.49 0.23 0 0 0 0.09 0.09 0.07 0 0 0 0 0 0
Nucleus 0.25 0.38 0.38 0.26 0.2 0.14 0.14 – 0.12 0.13 0.13 0.14 0.36 0.22 0.2 0.18 0.16 0.19 0.07 0.15 0.1 0.05 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.46 0.44 0.12 0.33 0.35 0.37 0.25 – 0.28 0.28 0.26 0.21 0.33 0.41 0.25 0.26 0.28 0.28 0.33 0.35 0.34 0.39 0.32 0.28
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.47 0.32 0.63 0.69 0.72 0.65 0.76 – 0.66 0.68 0.48 0.66 0.6 0.59 0.55 0.63 0.56 0.59 0.36 0.28 0.32 0.36 0.36 0.35
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 2 2 – 1 2 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 1 0 0 1 2 6 11 – 12 7 16 15 0 0 0 1 0 0 1 0 1 0 0 1
Bud Neck 2 0 9 6 18 16 33 – 18 21 17 21 5 5 6 1 0 0 2 1 3 0 2 4
Bud Site 3 0 0 3 4 4 11 – 14 12 25 17 0 0 0 0 1 2 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 9 9 6 12 18 20 25 – 16 18 17 21 16 54 71 15 8 15 1 3 0 2 2 4
Endoplasmic Reticulum 0 2 0 0 0 2 0 – 0 0 0 0 0 0 0 5 3 5 0 0 0 0 1 1
Endosome 26 20 19 14 13 15 12 – 6 5 3 15 11 28 59 54 40 59 36 18 31 6 21 28
Golgi 0 2 0 1 0 0 0 – 0 0 0 0 0 0 0 1 0 0 2 2 1 0 0 0
Mitochondria 49 9 2 8 5 32 14 – 69 40 179 81 2 0 1 17 11 12 8 4 3 0 4 5
Nucleus 69 59 78 57 57 45 51 – 35 37 47 48 73 69 63 33 20 32 22 29 26 4 13 7
Nuclear Periphery 6 1 0 0 0 1 1 – 1 3 0 0 2 0 0 0 0 0 3 1 0 0 2 1
Nucleolus 125 68 25 73 100 121 95 – 81 80 93 75 68 131 77 49 35 47 104 66 89 33 66 54
Peroxisomes 0 0 0 0 0 2 0 – 2 0 4 2 0 3 3 0 1 7 1 0 1 0 0 0
SpindlePole 128 49 127 152 205 212 283 – 192 195 174 236 122 186 169 116 71 101 115 54 84 30 73 66
Vac/Vac Membrane 2 3 2 1 3 0 0 – 0 0 2 0 2 2 6 6 4 7 8 5 12 3 10 9
Unique Cell Count 274 154 203 219 284 324 373 292 287 363 355 203 316 310 185 127 170 319 192 265 86 204 192
Labelled Cell Count 421 222 268 328 426 478 538 447 420 577 531 301 478 455 299 195 287 319 192 265 86 204 192


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.5 5.7 5.6 5.9 4.7 4.7 – 4.1 4.3 3.6 4.0 6.0 5.7 5.5 6.4 6.3 6.3 5.0 5.6 5.9
Std Deviation (1e-4) 0.5 0.8 0.9 1.5 1.2 0.9 1.1 – 1.4 1.1 0.8 1.1 1.0 1.0 1.3 1.3 1.0 1.2 1.0 1.2 1.3
Intensity Change (Log2) – – – -0.01 0.04 -0.28 -0.27 – -0.47 -0.4 -0.66 -0.5 0.06 0.01 -0.04 0.17 0.15 0.15 -0.19 -0.04 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck -0.9 0.9 0 0 – 0 0 0 0 -1.1 -2.0 -1.6 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.7 1.7 1.9 – 1.3 1.7 1.0 1.6 2.2 4.9 6.2 2.2 1.5 2.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome -1.1 -2.1 0 0 – 0 0 0 0 -1.5 -0.2 3.0 5.0 5.1 6.0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 3.7 3.5 3.1
Nucleus -2.7 -4.5 -6.5 -6.8 – -6.9 -6.6 -7.0 -6.8 -0.5 -4.1 -4.5 -4.5 -4.4 -4.1
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 5.1 5.7 0 0 – 0 0 0 0 5.1 7.1 3.5 3.5 3.5 3.7
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.5 2.2 0 0 – 0 0 0 0 -0.5 -0.8 -1.8 0 -1.2 -0.6
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9467 0.8791 0.5802 0.6165 0.5788 0.6663 0.652 0.3161 0.5727 0.4503 0.5056 0.4743 0.5484 0.2554 0.2228 0.5553 0.2604 -0.1094
Actin 0.0106 0.0001 0.0021 0.0026 0.0084 0.0019 0.0211 0.0004 0.0098 0.0013 0.0009 0.0025 0.0151 0.0001 0.0081 0.0026 0.0356 0.0011
Bud 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0.0003 0.0006 0.0003 0.0001 0.0001 0.0005 0.0003 0.0002 0.0001 0.0007 0.0001
Bud Neck 0.0027 0.0029 0.0014 0.0044 0.0018 0.0019 0.0058 0.0029 0.0035 0.0092 0.0065 0.0034 0.0041 0.0025 0.0063 0.0039 0.0064 0.0029
Bud Periphery 0.0004 0.0001 0.0001 0.0003 0.0003 0.0001 0.0009 0.0008 0.0014 0.0017 0.0003 0.0003 0.0024 0.0002 0.0003 0.0002 0.0014 0.0002
Bud Site 0.001 0.0012 0.0008 0.0022 0.0004 0.0002 0.0033 0.0041 0.0079 0.0014 0.0037 0.0008 0.003 0.0031 0.0017 0.0022 0.0019 0.0004
Cell Periphery 0.0001 0.0001 0.0001 0.0005 0.0001 0 0.0004 0.0006 0.0004 0.0009 0.0002 0.0001 0.0005 0.0002 0.0004 0.0003 0.0003 0.0001
Cytoplasm 0.0015 0.0009 0.0002 0.0001 0.0049 0.0002 0.0029 0.001 0.001 0.0002 0.0003 0.0004 0.0016 0.0033 0.0003 0.0007 0.0023 0.0002
Cytoplasmic Foci 0.0119 0.0032 0.0095 0.0145 0.0051 0.0044 0.0189 0.0117 0.0109 0.0068 0.0161 0.0087 0.0173 0.0089 0.0111 0.0147 0.0205 0.0063
Eisosomes 0.0003 0 0 0 0.0001 0.0001 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0003 0 0.0001 0.0001 0.0003 0
Endoplasmic Reticulum 0.0005 0.0001 0.0001 0 0.0018 0.0002 0.0029 0.0001 0.0006 0.0013 0.0001 0.0004 0.0019 0.0002 0.001 0.0012 0.002 0.0002
Endosome 0.0078 0.0023 0.0039 0.0035 0.0112 0.0023 0.035 0.0043 0.0108 0.0084 0.008 0.0082 0.018 0.0021 0.0077 0.0441 0.0586 0.0027
Golgi 0.0031 0.0006 0.0015 0.004 0.0062 0.0012 0.0128 0.0019 0.0046 0.0042 0.0061 0.0056 0.0087 0.0008 0.0046 0.0066 0.0337 0.002
Lipid Particles 0.0359 0.0091 0.023 0.0667 0.0213 0.0192 0.0571 0.0226 0.0315 0.0357 0.0941 0.0418 0.0818 0.0262 0.0521 0.0781 0.0708 0.0302
Mitochondria 0.0097 0.0002 0.0028 0.0015 0.0017 0.0008 0.004 0.0054 0.0194 0.0064 0.0028 0.0062 0.0169 0.0002 0.0208 0.0017 0.019 0.0008
None 0.0124 0.0054 0.0004 0.0001 0.0012 0.0003 0.0052 0.0113 0.0084 0.0008 0.0025 0.0007 0.0049 0.0122 0.0004 0.0006 0.0059 0.0002
Nuclear Periphery 0.0074 0.0051 0.0009 0.0004 0.0229 0.0033 0.0134 0.0026 0.0071 0.0117 0.004 0.0028 0.008 0.0076 0.005 0.005 0.0047 0.0052
Nucleolus 0.5623 0.5499 0.6056 0.69 0.3574 0.3799 0.4929 0.5614 0.5703 0.6018 0.324 0.4356 0.4231 0.6283 0.628 0.5549 0.359 0.4528
Nucleus 0.0888 0.1463 0.1177 0.0833 0.0596 0.0652 0.0901 0.1423 0.0922 0.0921 0.0656 0.072 0.0652 0.139 0.0887 0.0623 0.0803 0.0604
Peroxisomes 0.0065 0.0009 0.0028 0.022 0.0044 0.0017 0.0083 0.0086 0.0184 0.001 0.0307 0.0044 0.0169 0.0008 0.0181 0.0061 0.0092 0.002
Punctate Nuclear 0.2346 0.2691 0.224 0.1004 0.4895 0.5165 0.217 0.2104 0.1961 0.2076 0.432 0.4048 0.3032 0.1621 0.1416 0.2054 0.2802 0.4316
Vacuole 0.0014 0.0022 0.0027 0.003 0.0008 0.0003 0.0054 0.0063 0.0038 0.0053 0.0012 0.0008 0.0046 0.0015 0.0024 0.0068 0.0049 0.0004
Vacuole Periphery 0.0009 0.0004 0.0003 0.0004 0.0007 0.0002 0.002 0.0008 0.0014 0.0017 0.0004 0.0004 0.002 0.0003 0.001 0.0023 0.0023 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5268 8.6373 8.9729 12.0659 13.78 9.0129 13.0997 13.7533 14.871 14.1091
Translational Efficiency 0.8438 1.1081 0.9901 0.7722 0.6786 1.4013 0.8043 0.6776 0.6554 0.6784

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication
Localization
Cell Percentages nucleolus (71%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-1

Nsi1

Nsi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nsi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available