Standard name
Human Ortholog
Description Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.53 0.45 0.41 0.4 0.27 0.44 0.45 – 0.48 0.44 0.49 0.46 0.31 0.2 0.16 0.14 0.23 0.32 0.2 0.12 0 0 0 0
Bud 0.08 0.09 0.06 0.05 0 0.1 0.06 – 0.08 0.09 0.05 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.56 0.78 0.76 0.7 0.56 0.47 0.56 – 0.48 0.56 0.51 0.56 0.91 0.92 0.91 0.81 0.81 0.79 0.71 0.76 0.87 0.9 0.94 0.86
Cytoplasm 0.06 0.1 0.09 0.09 0.1 0.16 0.1 – 0.06 0.1 0.07 0.06 0 0 0 0.08 0.05 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0 0 0.07 0 0.08 0.09 – 0.12 0.05 0.11 0.09 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.12 0 0 0 0.35 0 0.2 – 0 0.14 0.11 0.16 0 0 0 0.12 0.16 0.08 0 0 0 0 0 0
Mitochondria 0.32 0.19 0.07 0.18 0 0.39 0.11 – 0.46 0.28 0.45 0.35 0.09 0.08 0.07 0.11 0.11 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 120 73 95 81 78 117 159 – 157 161 147 176 64 74 48 33 61 52 50 22 11 7 12 11
Bud 18 15 14 11 9 27 23 – 27 32 16 34 9 10 6 2 7 3 3 1 3 3 3 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 3 2 2 0 3
Bud Site 0 0 1 0 0 1 0 – 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 126 127 175 141 159 125 200 – 159 202 150 215 187 341 279 190 213 129 181 146 236 204 451 305
Cytoplasm 14 16 20 19 28 43 37 – 20 35 22 22 7 11 9 18 13 13 1 1 1 3 1 4
Endoplasmic Reticulum 13 3 6 14 5 22 32 – 40 17 32 33 5 13 10 16 4 11 10 3 3 1 5 13
Endosome 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Golgi 27 1 5 6 100 3 71 – 13 51 33 61 1 2 3 28 43 13 2 3 0 0 0 0
Mitochondria 73 31 15 37 7 105 39 – 151 100 133 133 18 29 23 26 29 20 1 4 5 1 3 3
Nucleus 0 0 0 1 1 1 0 – 1 2 1 1 0 0 0 1 1 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 1 – 3 0 1 0 0 0 0 0 0 0 0 0 1 0 0 1
Nucleolus 0 0 0 0 1 0 0 – 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 13 1 0 5 1 1 3 – 3 8 0 2 9 4 7 0 2 1 0 0 0 0 0 0
SpindlePole 0 2 7 7 16 1 2 – 0 6 0 2 1 0 0 0 1 3 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 6 16 1 5 – 0 7 0 4 0 0 0 1 2 1 2 1 1 0 1 2
Unique Cell Count 226 162 230 201 285 268 357 330 362 297 382 206 371 308 234 262 163 257 193 271 227 483 355
Labelled Cell Count 406 269 338 328 422 447 572 574 622 536 684 301 484 386 315 376 246 257 193 271 227 483 355


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.2 12.7 11.9 11.7 15.2 11.6 14.7 – 14.2 15.0 14.6 15.1 16.1 18.4 19.6 24.9 25.2 25.1 11.9 12.8 13.3
Std Deviation (1e-4) 2.2 2.5 2.9 1.9 5.4 3.0 2.6 – 2.8 3.0 3.3 3.5 2.7 3.3 3.9 6.3 6.8 6.7 8.6 6.3 6.5
Intensity Change (Log2) – – – -0.03 0.35 -0.04 0.3 – 0.25 0.33 0.3 0.35 0.43 0.63 0.72 1.06 1.08 1.07 0.0 0.1 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 -3.3 0.5 0.8 – 1.5 0.8 1.9 1.2 -2.2 -5.7 -6.7 -6.6 -4.3 -1.9
Bud -0.3 -1.6 1.6 0.2 – 0.9 1.2 -0.3 1.3 -0.8 -2.1 -2.5 -3.1 -1.9 -2.0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 -4.8 -6.7 -4.9 – -6.6 -5.0 -6.0 -4.9 4.1 5.4 4.6 1.3 1.4 0.7
Cytoplasm 0.3 0.4 2.5 0.7 – -1.2 0.4 -0.5 -1.4 -2.3 -3.1 -2.9 -0.4 -1.7 -0.3
Endoplasmic Reticulum 2.1 0 2.7 3.1 – 4.0 1.3 3.6 3.0 -0.1 0.6 0.4 2.1 0 2.0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0.5 9.2 0 6.2 – 1.2 4.8 3.9 5.3 0 0 0 4.1 5.3 2.7
Mitochondria 3.8 -2.3 8.5 1.8 – 10.0 6.3 9.7 7.9 0.9 0.6 0.4 1.7 1.8 2.0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.3 1.4 0 0 – 0 -1.1 0 0 0 0 0 0 0 0
Vacuole 0 3.7 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.7868 15.9796 12.9774 11.7799 9.973 12.4227 11.8754 22.629 19.3929 14.6615 14.4062 19.4236 14.7741 24.4687 19.2648 20.7567 17.3916 21.8525
Actin 0.1269 0.0035 0.0073 0.0036 0.0515 0.0436 0.1118 0.0302 0.0241 0.0398 0.032 0.0502 0.0838 0.0453 0.0874 0.0047 0.069 0.0065
Bud 0.0014 0.0002 0.0003 0.0004 0.0013 0.0004 0.0011 0.001 0.0006 0.0003 0.0017 0.0005 0.0017 0.0004 0.0012 0.0003 0.0004 0.0006
Bud Neck 0.0046 0.0033 0.0066 0.003 0.0057 0.002 0.0038 0.0042 0.0054 0.0098 0.015 0.0031 0.0109 0.0034 0.0111 0.0094 0.0019 0.0049
Bud Periphery 0.0056 0.0013 0.0025 0.0087 0.0053 0.0047 0.0065 0.0025 0.0049 0.0027 0.0111 0.0055 0.0053 0.0011 0.0039 0.0037 0.0029 0.0066
Bud Site 0.0274 0.063 0.0267 0.0007 0.0117 0.0015 0.0165 0.1032 0.0348 0.0054 0.0155 0.002 0.0368 0.1484 0.0305 0.0057 0.0026 0.0054
Cell Periphery 0.3021 0.4348 0.4347 0.615 0.3214 0.4947 0.4064 0.3805 0.4567 0.4963 0.4442 0.4582 0.3296 0.4604 0.4026 0.6177 0.3834 0.5939
Cytoplasm 0.0071 0.0072 0.0066 0.0049 0.0049 0.0033 0.0045 0.0072 0.0034 0.0024 0.0049 0.002 0.0115 0.0076 0.0112 0.0039 0.0061 0.0026
Cytoplasmic Foci 0.0078 0.0001 0.0002 0.0001 0.0196 0.0013 0.0016 0.0001 0.0002 0.0002 0.0109 0.0015 0.0094 0.003 0.0161 0.0001 0.0104 0.001
Eisosomes 0.4269 0.4792 0.4904 0.3286 0.3833 0.4205 0.4279 0.4631 0.4591 0.425 0.3072 0.4616 0.3567 0.2793 0.3454 0.3417 0.4196 0.3439
Endoplasmic Reticulum 0.0091 0.0027 0.0056 0.0264 0.0043 0.0055 0.0036 0.0019 0.0041 0.0062 0.0045 0.003 0.0079 0.0021 0.0136 0.006 0.0029 0.0044
Endosome 0.0143 0 0.0001 0.0001 0.0164 0.0016 0.0007 0 0 0.0001 0.0289 0.0006 0.0259 0.0005 0.0064 0.0001 0.0014 0.0047
Golgi 0.0061 0.0001 0.0022 0.0007 0.0175 0.0027 0.0032 0.0002 0.0003 0.001 0.0142 0.0021 0.0156 0.0018 0.0055 0.0007 0.0061 0.0045
Lipid Particles 0.0046 0.0001 0.0011 0.0005 0.0256 0.0028 0.0018 0.0002 0.0003 0.0004 0.0394 0.0011 0.0131 0.0021 0.0136 0.0004 0.0089 0.0011
Mitochondria 0.0238 0.0016 0.012 0.0022 0.093 0.0109 0.004 0.0032 0.0023 0.0059 0.0231 0.0044 0.0413 0.0275 0.0287 0.0016 0.0113 0.0158
None 0.0041 0.0009 0.0008 0.0012 0.0065 0.0009 0.0011 0.0006 0.0008 0.0011 0.0029 0.0008 0.0056 0.0008 0.0083 0.001 0.0012 0.0007
Nuclear Periphery 0.0134 0.0008 0.0009 0.0021 0.0041 0.0008 0.0009 0.0006 0.001 0.001 0.0198 0.0007 0.0093 0.0007 0.0025 0.001 0.0005 0.0008
Nucleolus 0.0006 0.0001 0.0001 0.0001 0.0009 0.0001 0.0002 0.0001 0.0001 0.0001 0.0014 0.0001 0.0009 0.0001 0.0007 0.0001 0.0001 0.0001
Nucleus 0.0024 0.0001 0.0001 0.0001 0.0011 0.0002 0.0002 0.0001 0.0001 0.0001 0.0015 0.0001 0.0189 0.0001 0.0006 0.0001 0.0001 0.0001
Peroxisomes 0.0021 0.0001 0.0003 0 0.0112 0.0011 0.0005 0.0001 0.0002 0.0002 0.0069 0.0009 0.0038 0.0139 0.0055 0.0001 0.0701 0.0003
Punctate Nuclear 0.005 0 0 0 0.0084 0.0002 0.0007 0 0 0 0.0034 0.0001 0.0072 0.0001 0.0019 0 0.0004 0
Vacuole 0.003 0.0008 0.0012 0.0015 0.0051 0.0013 0.0028 0.0009 0.0015 0.0019 0.0048 0.0015 0.0033 0.0013 0.0029 0.0016 0.0006 0.0018
Vacuole Periphery 0.0017 0 0.0001 0.0001 0.0011 0.0001 0.0002 0 0.0001 0.0001 0.0068 0.0001 0.0016 0.0001 0.0007 0.0001 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 158.9494 153.6566 144.6703 157.9124 176.1306 169.9447 184.5968 187.7312 267.2033 213.2288
Translational Efficiency 2.7411 2.2176 2.2852 2.7339 2.4416 3.061 2.5126 2.8096 2.1075 2.6742

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (83%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-2

Pst2

Pst2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pst2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available