Standard name
Human Ortholog
Description 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0
Bud Site 0 0
Cell Periphery 0 0 0 0 0 0 0 0
Cytoplasm 0.24 0.95 0.08 0.61 0 0 0.18 0.77
Endoplasmic Reticulum 0 0 0 0 0 0.47 0 0
Endosome 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.06 0.28 0.13
Nucleus 0.34 0 0.77 0.26 0.99 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0.09 0
Peroxisomes 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.06
Vac/Vac Membrane 0.29 0 0 0.07 0 0 0.17 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0
Bud 0 0 3 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0
Bud Site 0 0
Cell Periphery 0 0 0 0 0 0 0 0
Cytoplasm 10 39 7 54 0 0 0 0
Endoplasmic Reticulum 1 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0
Mitochondria 1 0 0 0 0 0 1 0
Nucleus 14 0 69 23 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0
Nucleolus 3 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0
Vac/Vac Membrane 12 1 3 6 0 0 0 0
Unique Cell Count 41 41 90 89 1 2 4 1
Labelled Cell Count 41 41 90 89 1 2 4 1


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 44.2 83.2
Std Deviation (1e-4) 15.1 18.0
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0438 0 0.0004 0 0.0002 0 0.0167 0 0.0035 0.0004 0 0.0001
Bud 0.0014 0 0 0.001 0.0003 0.0001 0.0004 0.0002 0.0019 0.0001 0.0001 0.0016
Bud Neck 0.0016 0 0.0001 0.0001 0.0006 0.0007 0.0008 0.0001 0.0006 0.0002 0.0006 0.0035
Bud Periphery 0.0011 0 0 0.0003 0.0003 0.0001 0.0001 0 0.0008 0.0001 0.0001 0.0013
Bud Site 0.0076 0 0 0.0001 0.0003 0.0001 0.0015 0.0003 0.0019 0 0.0001 0.0004
Cell Periphery 0.0019 0.0001 0 0.0002 0.0009 0.0003 0.0004 0.0001 0.0001 0 0.0002 0.0003
Cytoplasm 0.0078 0.0008 0.0003 0.0026 0.0015 0.0002 0.4051 0.0211 0.0277 0.004 0.0006 0.0036
Cytoplasmic Foci 0.0598 0 0.0001 0 0.0148 0 0.0055 0.0007 0.0014 0 0 0
Eisosomes 0.0005 0 0.0001 0 0.0001 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0029 0 0 0 0.0001 0 0.0176 0.0001 0.0004 0.0008 0 0.0001
Endosome 0.0199 0 0.0003 0 0.0004 0 0.0082 0.0001 0.0016 0.0002 0 0.0001
Golgi 0.0208 0 0.0002 0 0.0001 0 0.0012 0 0.0006 0 0 0
Lipid Particles 0.0934 0 0.0018 0 0.0024 0 0.0013 0.0001 0 0 0 0
Mitochondria 0.018 0.0001 0.0011 0.0001 0.0047 0.0002 0.0019 0.0002 0.005 0.0006 0.0003 0.0002
None 0.0056 0 0.0001 0.0001 0.0026 0 0.0098 0.0001 0.0002 0.0001 0 0
Nuclear Periphery 0.0056 0.0053 0.0072 0.005 0.0055 0.001 0.1378 0.0141 0.0095 0.0338 0.0047 0.0072
Nucleolus 0.2829 0.0046 0.0043 0.0049 0.175 0.0554 0.0021 0.0052 0.0051 0.0016 0.0497 0.0359
Nucleus 0.3069 0.9888 0.9828 0.9843 0.7791 0.9404 0.3576 0.9554 0.9349 0.9564 0.9424 0.9399
Peroxisomes 0.0766 0 0.0004 0 0.0003 0 0.0002 0 0.0008 0 0 0
Punctate Nuclear 0.0264 0 0.0004 0.0002 0.004 0.0004 0.0224 0.0002 0.0009 0.0003 0.0002 0.0004
Vacuole 0.0079 0.0001 0.0001 0.0009 0.0032 0.0007 0.0059 0.0015 0.0021 0.0007 0.0006 0.0047
Vacuole Periphery 0.0076 0.0001 0.0001 0.0002 0.0037 0.0003 0.0032 0.0004 0.0009 0.0006 0.0004 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 153.6637 132.0486 142.7041 146.6482 156.8146 149.6153 155.0198 145.7533 151.6906 141.2011
Translational Efficiency 1.4217 1.6251 1.483 1.7443 1.5071 1.5719 1.3337 1.2816 1.2987 1.2573

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
Localization
Cell Percentages cytoplasm (28%), nucleus (3%), mixed (67%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Aro3

Aro3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aro3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available