Standard name
Human Ortholog
Description 3-hydroxyisobutyryl-CoA hydrolase; member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.13 0.16 0 0 0 0 0 0
Bud 0 0 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0.08 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0 0 0 0.07 0.07 0.06 0.08 0.07 0.06
Golgi 0 0.07 0 0 0 0 0 0 0 0 0 0 0.26 0.32 0.25 0.7 0.79 0.73 0.09 0.12 0.08 0.1 0.11 0.06
Mitochondria 0.95 0.79 0.95 0.94 0.86 0.93 0.89 0.87 0.94 0.93 0.95 0.92 0.79 0.76 0.73 0.12 0.2 0.07 0.7 0.61 0.67 0.69 0.63 0.65
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0 0.06 0.07 0.05 0.09 0.09 0.06 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0.05 0.07 0.1 0.1 0.09 0.1 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0 0 0 0 0 0 0 0 0 0.06 0.06 0.09 0 0 0 0.05 0 0.12 0.06 0.07 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.2 0 0 0.14 0.05 0.05 0.07 0 0.05 0 0 0.13 0.09 0.11 0.19 0.21 0.21 0 0.06 0 0 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 3 1 1 1 2 1 2 4 1 1 2 2 2 39 49 51 5 25 9 6 19 16
Bud 4 4 11 28 4 13 14 21 11 13 13 7 8 7 1 6 2 2 0 7 1 2 1 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 7
Bud Site 0 1 2 5 1 4 6 6 5 3 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 22 7 4 7 2 1 1 3 4 1 4 30 15 22 26 28 14 0 0 1 0 0 1
Cytoplasm 1 8 7 4 7 8 4 9 4 8 8 10 15 13 10 16 17 20 0 1 0 0 1 1
Endoplasmic Reticulum 1 5 2 2 4 6 0 4 4 7 4 11 12 11 7 8 18 10 0 2 0 1 2 1
Endosome 0 25 8 9 13 9 13 12 4 4 0 1 31 37 42 2 1 2 12 44 19 32 37 33
Golgi 1 35 5 3 9 2 2 2 2 6 5 2 155 202 153 225 291 235 15 74 26 41 63 32
Mitochondria 167 416 321 233 255 362 399 462 380 384 300 340 468 489 452 37 73 22 120 373 223 295 354 336
Nucleus 0 7 0 0 4 3 4 3 0 0 1 0 0 1 0 4 1 3 0 4 0 0 3 1
Nuclear Periphery 9 13 13 6 11 23 33 28 36 36 19 36 9 11 6 2 3 0 1 3 2 4 8 6
Nucleolus 12 13 9 10 14 26 44 53 36 42 16 22 7 3 4 14 11 12 0 4 1 2 1 1
Peroxisomes 0 15 19 10 7 3 9 8 4 3 1 0 33 37 57 4 1 1 8 19 41 26 41 22
SpindlePole 0 7 2 5 2 2 9 3 3 4 2 0 3 6 3 3 5 6 1 3 1 2 3 4
Vac/Vac Membrane 17 103 12 6 42 18 21 39 14 19 7 15 76 55 68 62 79 68 4 39 2 7 15 43
Unique Cell Count 175 525 339 249 298 390 447 529 404 414 315 369 591 640 616 321 370 323 171 608 334 429 561 521
Labelled Cell Count 213 680 421 326 381 482 561 652 508 537 379 450 849 889 827 448 579 446 171 608 334 429 561 521


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 8.9 6.8 6.1 7.1 6.8 6.7 7.0 7.2 7.1 6.6 7.3 8.4 8.2 8.0 20.8 25.8 24.7 6.8 6.9 7.7
Std Deviation (1e-4) 1.5 2.1 1.4 1.3 1.6 1.5 1.7 1.7 1.6 1.7 1.6 1.7 1.7 1.7 1.7 7.4 9.7 9.5 1.4 1.6 1.7
Intensity Change (Log2) – – – -0.17 0.05 -0.01 -0.02 0.04 0.08 0.06 -0.06 0.1 0.3 0.27 0.23 1.61 1.92 1.86 -0.01 0.02 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 5.9 6.3 7.0
Bud 3.9 -1.6 0.1 -0.1 0.6 -0.4 -0.1 0.6 -1.1 -2.0 -2.4 0 -1.1 -2.7 -2.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.2 0 0 0 0 0 0 -1.1 2.3 0.3 1.3 3.6 3.4 1.7
Cytoplasm 0 0.2 0 0 -0.4 -1.2 -0.1 0.4 0.6 0.5 0 -0.5 2.0 1.9 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 2.4 1.7 0 0 0 3.4 2.4
Endosome 0.9 1.4 0 0.5 -0.1 -1.5 -1.5 0 0 2.1 2.4 3.0 0 0 0
Golgi 0 1.3 0 0 0 0 0 0 0 9.6 11.0 9.3 18.5 20.8 19.1
Mitochondria -0.6 -3.9 -1.0 -2.7 -3.6 -0.4 -1.1 0.3 -1.4 -6.3 -7.2 -8.0 -21.4 -20.1 -22.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.0 -0.1 1.3 2.1 1.0 2.8 2.7 1.3 3.1 -2.2 -2.0 -3.0 -2.8 -2.7 -3.6
Nucleolus 0.9 1.4 2.5 4.0 4.1 3.6 4.1 1.6 2.1 -1.7 0 0 1.2 0.3 0.8
Peroxisomes -0.9 -2.1 -3.8 -2.7 -3.4 -3.6 -4.0 -3.9 -4.6 0 0.1 2.0 -3.1 -4.3 -4.0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.8 4.8 0.7 0.8 2.3 -0.1 0.7 -1.0 0.4 4.7 3.0 4.0 6.4 7.1 6.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.0798 9.0604 9.3565 9.3571 8.4907 9.1194 9.7672 8.9394 8.9162 8.0154 7.7627 9.0742 9.1347 9.2459 9.736 10.1544 9.8682 9.8921
Actin 0.0109 0.0162 0.0184 0.0008 0.0105 0.0064 0.0236 0.0222 0.0245 0.003 0.0422 0.0051 0.0048 0.0175 0.0053 0.0032 0.003 0.0057
Bud 0.0003 0.0046 0.0011 0.0003 0.0004 0.0002 0.0007 0.0012 0.0008 0.0004 0.0019 0.0002 0.0004 0.0018 0.0003 0.0027 0.0003 0.0029
Bud Neck 0.0003 0.0003 0.0009 0.0004 0.0002 0.0002 0.0003 0.0002 0.0003 0.0003 0.0005 0.0002 0.0004 0.0006 0.0002 0.0002 0.0001 0.0002
Bud Periphery 0.0009 0.0024 0.002 0.0011 0.0006 0.0004 0.002 0.002 0.001 0.0009 0.0028 0.0004 0.0005 0.0033 0.0006 0.0061 0.0011 0.0007
Bud Site 0.0011 0.0087 0.0084 0.0003 0.0009 0.0003 0.001 0.0015 0.0053 0.0007 0.0085 0.0002 0.0056 0.0044 0.0009 0.0028 0.0001 0.0003
Cell Periphery 0.0005 0.0009 0.0006 0.0004 0.0001 0.0002 0.0003 0.0002 0.0003 0.0003 0.0004 0.0001 0.0004 0.0005 0.0002 0.0006 0.0001 0.0002
Cytoplasm 0.0011 0.002 0.0008 0.0001 0.0024 0.0006 0.0006 0.0004 0.0006 0.0003 0.0005 0.0013 0.0013 0.0005 0.0003 0.0002 0.0001 0.0006
Cytoplasmic Foci 0.0096 0.014 0.0131 0.0014 0.011 0.0067 0.0109 0.0151 0.0071 0.0052 0.0068 0.0132 0.0126 0.0124 0.0093 0.0112 0.0053 0.0053
Eisosomes 0.0007 0.0004 0.0004 0.0001 0.0002 0.0002 0.0004 0.0004 0.0004 0.0001 0.0007 0.0002 0.0003 0.0004 0.0002 0.0002 0.0002 0.0002
Endoplasmic Reticulum 0.0012 0.0006 0.0006 0.0001 0.0025 0.0004 0.0007 0.0002 0.0011 0.0002 0.0017 0.0003 0.0002 0.0005 0.0005 0.0001 0.0004 0.0024
Endosome 0.0218 0.0355 0.0253 0.0063 0.0429 0.0176 0.0277 0.041 0.0239 0.0176 0.0431 0.0183 0.0314 0.0285 0.0274 0.0031 0.0351 0.0182
Golgi 0.0433 0.0476 0.0314 0.0117 0.0917 0.0267 0.0479 0.077 0.0398 0.0259 0.07 0.0524 0.0549 0.0546 0.0565 0.0054 0.0792 0.0499
Lipid Particles 0.0196 0.0146 0.0108 0.001 0.0074 0.0087 0.0285 0.0066 0.0107 0.0031 0.0128 0.0049 0.0216 0.0113 0.0063 0.0094 0.0023 0.0096
Mitochondria 0.8548 0.816 0.8427 0.957 0.8067 0.9002 0.831 0.8077 0.8559 0.9343 0.7855 0.8915 0.8435 0.8421 0.867 0.9288 0.8448 0.8834
None 0.0005 0.0002 0.0005 0 0.0007 0.0001 0.0014 0.0001 0.0002 0.0001 0.0008 0.0001 0.0003 0.0002 0.0001 0.0007 0.0001 0.0006
Nuclear Periphery 0.0005 0.0005 0.0018 0 0.0009 0.0004 0.0022 0.0001 0.0004 0.0001 0.0026 0.0001 0.0001 0.0004 0.0002 0.0001 0.0001 0.0018
Nucleolus 0.0002 0.0009 0.0005 0.0001 0.0001 0.0001 0.0008 0.0001 0.0002 0.0001 0.0006 0.0001 0.0007 0.0007 0.0004 0.004 0 0.0006
Nucleus 0.0003 0.0006 0.0004 0.0001 0.0002 0.0001 0.0007 0.0001 0.0001 0.0001 0.0007 0 0.0003 0.0003 0.0003 0.0007 0 0.0007
Peroxisomes 0.0238 0.0155 0.0276 0.0133 0.0152 0.0259 0.0085 0.0197 0.0241 0.0043 0.0094 0.0085 0.0132 0.012 0.0181 0.004 0.0222 0.0132
Punctate Nuclear 0.0018 0.0001 0.0002 0 0.0003 0.0001 0.0049 0.0001 0.0002 0 0.001 0 0.0001 0.0001 0 0.0015 0 0.0001
Vacuole 0.003 0.0107 0.0048 0.0024 0.0016 0.0011 0.0023 0.0014 0.0011 0.0017 0.0027 0.0005 0.0026 0.0034 0.0022 0.0116 0.0014 0.0011
Vacuole Periphery 0.004 0.0077 0.0075 0.0031 0.0034 0.0031 0.0037 0.0025 0.0022 0.0014 0.0048 0.0022 0.0048 0.0047 0.0037 0.0031 0.0041 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.3019 22.3132 23.3751 32.582 21.8923 25.6805 35.9979 34.2609 30.8585 37.1568
Translational Efficiency 1.1498 1.596 1.4538 0.9652 1.5343 1.6385 1.1677 1.1957 1.1795 1.1268

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description 3-hydroxyisobutyryl-CoA hydrolase; member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis
Localization
Cell Percentages mitochondrion (92%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Ehd3

Ehd3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ehd3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available