Standard name
Human Ortholog
Description Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress; co-expression of human CDC7 and DBF4 complements single cdc7 or dbf4 null mutations or the cdc7 dbf4 double null mutation
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0 0.05 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.68 0.71 0.84 0.81 0.8 0.84 0.79 0.84 0.7 – 0.64 0.68 0.62 0.66 0.7 0.81 0.67 0.63 0.48 0.52 0.49 0.68 0.62 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.09 0 0 0 0.13 0.11 0.07 0.31 – 0.38 0.25 0.05 0 0 0 0.06 0 0.05 0 0.1 0.17 0.19 0.12
Nucleus 0.17 0.15 0.11 0.15 0.13 0.08 0.11 0.07 0.08 – 0.11 0.09 0.33 0.25 0.15 0.12 0.2 0.05 0.22 0.22 0.14 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0.07 0 0.05 0 0 0
Vac/Vac Membrane 0.09 0.11 0 0 0 0 0 0 0 – 0 0 0 0.1 0.14 0.19 0.16 0.26 0.1 0.11 0.12 0 0.06 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 2 0 2 0 0 – 0 1 0 0 0 0 0 0 2 3 5 0 7 6
Bud 0 0 0 0 2 1 6 1 7 – 9 7 1 0 0 0 0 0 5 5 3 4 4 10
Bud Neck 5 2 23 9 13 17 33 27 18 – 6 18 0 0 0 0 0 0 0 1 1 0 5 6
Bud Site 0 0 0 0 0 1 1 1 6 – 1 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 11 1 4 1 1 2 2 – 1 0 1 0 0 0 0 0 1 0 4 1 7 8
Cytoplasm 198 138 336 268 353 472 490 447 377 – 341 320 210 311 410 13 55 12 168 106 216 116 258 362
Endoplasmic Reticulum 1 1 1 1 1 0 2 0 0 – 0 1 2 0 2 0 5 1 1 0 1 2 4 4
Endosome 0 0 0 0 0 0 0 2 0 – 0 1 0 6 8 0 2 2 4 2 2 1 3 1
Golgi 0 0 0 2 0 0 0 1 0 – 0 0 0 0 1 0 0 0 3 1 0 1 1 1
Mitochondria 24 17 1 5 18 74 71 35 168 – 203 117 18 7 9 0 5 0 18 4 44 29 78 59
Nucleus 51 30 43 50 58 47 67 35 45 – 58 40 113 116 86 2 16 1 77 46 62 1 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 1 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 2 5 8 12 10 – 6 23 1 0 0 0 1 0 24 5 22 1 4 8
Vac/Vac Membrane 26 22 3 2 16 9 2 7 1 – 9 7 13 45 84 3 13 5 36 23 50 7 22 20
Unique Cell Count 292 195 398 331 442 564 620 532 540 537 469 341 468 582 16 82 19 352 206 439 171 414 505
Labelled Cell Count 306 211 420 338 469 627 683 570 635 634 540 360 486 601 18 98 21 352 206 439 171 414 505


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.9 4.6 4.1 4.6 3.5 3.7 4.1 3.1 – 3.1 3.7 4.1 4.1 4.2 6.5 6.4 7.1 3.7 3.6 3.9
Std Deviation (1e-4) 0.5 1.3 0.7 0.9 1.2 0.7 0.7 1.1 0.7 – 0.7 1.0 1.2 1.0 1.3 2.6 2.0 2.5 1.5 1.5 1.3
Intensity Change (Log2) – – – -0.16 0.02 -0.4 -0.3 -0.17 -0.54 – -0.56 -0.31 -0.15 -0.16 -0.13 0.52 0.48 0.63 -0.3 -0.35 -0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.6741 -1.2041 -1.2859 -1.3979 -1.3775 -1.6585 -0.4564 -0.3414 0.0318 -0.209 0.2986 -0.4837 -0.5254 -0.5904 -0.2752 -0.2903 -0.4447 -0.7641
Actin 0.0391 0.0009 0.0042 0.0005 0.0008 0.0014 0.0261 0.0003 0.0127 0.0003 0.0138 0.0084 0.0165 0.0001 0.0087 0.0185 0.0015 0.0106
Bud 0.0025 0.0006 0.0012 0.0001 0 0.0005 0.0032 0.0026 0.0009 0.0001 0.0035 0.001 0.0013 0.0001 0.0004 0.0003 0.0004 0.0002
Bud Neck 0.0141 0.0042 0.0017 0.0006 0.0004 0.0023 0.0064 0.0004 0.0009 0.0009 0.0026 0.0031 0.0033 0.0002 0.0006 0.0025 0.0007 0.0008
Bud Periphery 0.0058 0.0006 0.0011 0.0001 0 0.0005 0.0041 0.0024 0.0016 0.0001 0.0081 0.0027 0.0033 0.0001 0.0004 0.0004 0.0019 0.0012
Bud Site 0.0054 0.0119 0.0071 0.0001 0 0.0002 0.0255 0.0036 0.0051 0.0005 0.0078 0.0006 0.0183 0.0006 0.0104 0.0086 0.0003 0.0001
Cell Periphery 0.0007 0.0002 0.0001 0 0 0 0.001 0.0003 0.0002 0 0.0006 0.0002 0.0013 0.0001 0.0002 0.0003 0.0001 0.0001
Cytoplasm 0.07 0.122 0.1163 0.162 0.1619 0.1199 0.1795 0.1955 0.1187 0.1073 0.1528 0.28 0.0794 0.1544 0.0818 0.0667 0.0997 0.1493
Cytoplasmic Foci 0.0311 0.0201 0.0122 0.0043 0.0171 0.0173 0.0314 0.0038 0.0119 0.014 0.0362 0.0185 0.0315 0.0034 0.0032 0.0016 0.0182 0.021
Eisosomes 0.0011 0.0002 0.0001 0.0001 0.0001 0.0001 0.0008 0.0001 0.0001 0 0.0003 0.0002 0.0016 0.0001 0.0001 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0083 0.0061 0.0035 0.0031 0.0016 0.0017 0.0097 0.002 0.0022 0.0026 0.005 0.0033 0.0048 0.0015 0.0015 0.0009 0.0017 0.0032
Endosome 0.0205 0.0044 0.0049 0.0032 0.0027 0.0026 0.0414 0.0019 0.013 0.0122 0.1232 0.0059 0.0389 0.0013 0.0026 0.0012 0.0136 0.0084
Golgi 0.0059 0.0004 0.0008 0.0001 0.0001 0.0002 0.008 0.0001 0.0081 0.0012 0.0726 0.002 0.0179 0.0001 0.0015 0.0004 0.0016 0.0041
Lipid Particles 0.0119 0.0013 0.0041 0.0006 0.0003 0.0006 0.0117 0.0002 0.0044 0.0004 0.0177 0.007 0.0355 0.0007 0.0013 0.0003 0.0026 0.0067
Mitochondria 0.0034 0.0004 0.0011 0.0008 0.0002 0.0006 0.0066 0.0006 0.0113 0.0013 0.034 0.0072 0.0359 0.0003 0.0011 0.0011 0.0061 0.0028
None 0.6881 0.6003 0.3447 0.4134 0.6937 0.7707 0.5468 0.5003 0.2142 0.1338 0.2268 0.5508 0.5973 0.4992 0.3587 0.1991 0.6185 0.6807
Nuclear Periphery 0.0135 0.0101 0.016 0.0139 0.0037 0.005 0.0125 0.0059 0.018 0.017 0.0135 0.0061 0.0104 0.0078 0.0214 0.0406 0.0137 0.0066
Nucleolus 0.0047 0.0029 0.0052 0.0046 0.0006 0.0008 0.0078 0.0022 0.0031 0.0077 0.0031 0.001 0.0048 0.0015 0.0053 0.0045 0.005 0.001
Nucleus 0.0382 0.1877 0.4229 0.3161 0.083 0.0622 0.0413 0.2631 0.5284 0.6708 0.2214 0.084 0.0469 0.3199 0.4805 0.6171 0.1507 0.0793
Peroxisomes 0.0055 0.0041 0.0032 0.0005 0.0006 0.0007 0.0057 0.0001 0.0061 0.001 0.0269 0.0096 0.0316 0.0001 0.0006 0.0003 0.0367 0.0116
Punctate Nuclear 0.021 0.0193 0.0462 0.073 0.0325 0.0113 0.01 0.0121 0.0325 0.0267 0.0138 0.0049 0.0108 0.0073 0.0183 0.0342 0.0215 0.0102
Vacuole 0.0069 0.0021 0.0029 0.0025 0.0004 0.0012 0.0173 0.0023 0.0054 0.0016 0.0113 0.0026 0.0062 0.0011 0.0011 0.0009 0.0031 0.0011
Vacuole Periphery 0.0023 0.0002 0.0006 0.0004 0.0001 0.0002 0.0034 0.0004 0.0015 0.0005 0.0051 0.0009 0.0026 0.0001 0.0004 0.0006 0.0023 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.6941 21.9195 18.6877 23.0603 24.6891 23.9851 19.6871 18.7775 18.9389 25.573
Translational Efficiency 1.1895 0.8632 0.7471 0.8865 0.8469 1.057 0.7395 0.774 0.7455 0.7941

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress; co-expression of human CDC7 and DBF4 complements single cdc7 or dbf4 null mutations or the cdc7 dbf4 double null mutation
Localization
Cell Percentages cytoplasm (24%), nucleus (7%), mixed (47%)
Cell Cycle Regulation Yes
cytoplasm - T/G1 (7.6e-05)
nucleus - S/G2 (6.5e-06)
Subcompartmental Group N/A

Dbf4

Dbf4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dbf4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available