Standard name | |
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Human Ortholog | |
Description | Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress; human CDC34 functionally complements the thermosensitivity of the cdc34-2 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 12 | 2 | 1 | 4 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 3 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 56 | 63 | 14 | 6 | 19 | 23 | 43 | 36 | 25 | 39 | 20 | 28 | 30 | 34 | 51 | 24 | 23 | 17 | 14 | 23 | 13 | 4 | 9 | 9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 11 | 5 | 6 | 0 | 0 | 0 | 0 | 1 | 4 |
Endosome | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 3 | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 2 | 3 | 4 | 1 | 1 | 0 | 4 |
Golgi | 1 | 2 | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 4 | 1 | 2 | 4 | 0 | 3 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3 | 12 | 3 | 63 | 60 | 57 | 57 | 0 | 0 | 1 | 4 | 4 | 0 | 0 | 2 | 1 | 0 | 1 | 0 |
Nucleus | 117 | 152 | 63 | 58 | 102 | 80 | 126 | 69 | 98 | 138 | 69 | 79 | 59 | 61 | 57 | 136 | 96 | 58 | 195 | 347 | 119 | 45 | 96 | 111 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 2 | 0 | 0 | 1 | 2 | 1 | 1 | 8 | 2 | 2 | 4 | 0 | 1 | 0 | 2 | 0 | 2 | 2 | 2 | 2 | 4 | 12 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 4 |
Vac/Vac Membrane | 136 | 264 | 69 | 19 | 45 | 56 | 93 | 38 | 60 | 66 | 26 | 50 | 46 | 63 | 72 | 57 | 69 | 68 | 10 | 34 | 11 | 4 | 13 | 25 |
Unique Cell Count | 237 | 396 | 133 | 80 | 149 | 147 | 251 | 134 | 207 | 281 | 150 | 186 | 129 | 147 | 172 | 204 | 175 | 135 | 242 | 436 | 157 | 62 | 137 | 183 |
Labelled Cell Count | 311 | 483 | 151 | 83 | 166 | 166 | 278 | 151 | 254 | 325 | 178 | 221 | 144 | 161 | 187 | 233 | 202 | 155 | 242 | 436 | 157 | 62 | 137 | 183 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.4 | 13.1 | 13.3 | 12.4 | 12.3 | 8.8 | 8.0 | 7.7 | 6.2 | 6.7 | 5.7 | 6.3 | 14.2 | 15.1 | 14.0 | 13.0 | 16.4 | 18.4 | – | 14.2 | 15.1 |
Std Deviation (1e-4) | 2.2 | 2.4 | 2.3 | 2.3 | 2.4 | 1.6 | 1.3 | 1.4 | 1.2 | 1.1 | 0.9 | 1.1 | 3.4 | 2.9 | 2.5 | 3.1 | 4.9 | 5.0 | – | 3.2 | 3.3 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.12 | -0.59 | -0.74 | -0.79 | -1.11 | -0.99 | -1.22 | -1.07 | 0.09 | 0.18 | 0.07 | -0.04 | 0.3 | 0.47 | – | 0.09 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.7 | 0.6 | 1.3 | 1.7 | 3.4 | 0.4 | 1.0 | 0.7 | 1.2 | 2.8 | 2.8 | 4.0 | 0.4 | 0.7 | 0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 7.0 | 5.8 | 8.0 | 7.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 3.6 | 3.6 | 1.2 | 0.5 | 0.7 | 0 | 0.3 | -0.2 | -0.9 | -0.3 | -1.0 | -2.5 | 3.5 | 1.3 | -0.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.0 | -3.7 | -2.3 | -2.8 | -3.9 | -4.2 | -5.8 | -6.1 | -4.6 | -2.6 | -1.5 | -1.7 | -4.4 | -2.2 | -0.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.6613 | 11.6106 | 10.3441 | 9.165 | 8.0165 | 10.086 | 9.6165 | 11.9569 | 11.1888 | 9.9541 | 9.5661 | 11.0246 | 8.5174 | 10.7558 | 9.6637 | 9.257 | 8.8275 | 9.5198 |
Actin | 0.016 | 0 | 0.0077 | 0.0104 | 0.004 | 0 | 0.0357 | 0 | 0.0113 | 0.0244 | 0 | 0.0001 | 0.0028 | 0.0002 | 0.0005 | 0 | 0 | 0 |
Bud | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.001 | 0 | 0.0004 | 0 | 0.0011 | 0.0008 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0011 | 0.0004 | 0.002 | 0.0006 | 0.0015 | 0.0013 | 0.0011 | 0.0002 | 0.0006 | 0.0007 | 0.0001 | 0.0011 | 0.0014 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0009 |
Bud Periphery | 0.0007 | 0 | 0.0007 | 0.0003 | 0.0027 | 0 | 0.0005 | 0 | 0.001 | 0.0012 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.004 | 0.0002 | 0.0063 | 0.0032 | 0.0013 | 0 | 0.0021 | 0.0002 | 0.0175 | 0.0024 | 0 | 0 | 0.0002 | 0.0009 | 0.0001 | 0 | 0 | 0 |
Cell Periphery | 0.0007 | 0.0001 | 0.0005 | 0.001 | 0.0008 | 0 | 0.0002 | 0 | 0.0001 | 0.0005 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0167 | 0.0468 | 0.012 | 0.0055 | 0.013 | 0.0018 | 0.0268 | 0.0235 | 0.0109 | 0.0224 | 0.0083 | 0.0035 | 0.0068 | 0.0057 | 0.0049 | 0.0027 | 0.0008 | 0.0023 |
Cytoplasmic Foci | 0.039 | 0.0002 | 0.0019 | 0.0031 | 0.0025 | 0 | 0.0269 | 0 | 0.013 | 0.0186 | 0 | 0.0007 | 0.0137 | 0 | 0.0005 | 0 | 0 | 0 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0.0022 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0012 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0062 | 0.0002 | 0.0002 | 0.0038 | 0.006 | 0.0001 | 0.007 | 0.0002 | 0.0038 | 0.0092 | 0.0001 | 0.0002 | 0.0025 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 |
Endosome | 0.0234 | 0.0003 | 0.0018 | 0.0012 | 0.0193 | 0 | 0.0116 | 0.0001 | 0.0073 | 0.0386 | 0.0001 | 0.0006 | 0.0104 | 0 | 0.001 | 0 | 0 | 0 |
Golgi | 0.0102 | 0 | 0.0011 | 0.0011 | 0.025 | 0 | 0.0023 | 0 | 0.0012 | 0.0073 | 0 | 0 | 0.001 | 0 | 0.0002 | 0 | 0 | 0 |
Lipid Particles | 0.0202 | 0 | 0.0013 | 0.0024 | 0.0036 | 0 | 0.0051 | 0 | 0.0032 | 0.0164 | 0 | 0.0001 | 0.0014 | 0 | 0.0005 | 0 | 0 | 0 |
Mitochondria | 0.0049 | 0.0001 | 0.004 | 0.0008 | 0.0397 | 0.0001 | 0.0013 | 0.0001 | 0.0008 | 0.029 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
None | 0.0044 | 0 | 0.0005 | 0.0159 | 0.0028 | 0 | 0.0133 | 0 | 0.0006 | 0.0348 | 0 | 0.0005 | 0.0027 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Nuclear Periphery | 0.0218 | 0.0048 | 0.0035 | 0.0036 | 0.0216 | 0.0026 | 0.014 | 0.0062 | 0.0058 | 0.0234 | 0.0072 | 0.0039 | 0.0094 | 0.0034 | 0.003 | 0.0039 | 0.0021 | 0.0035 |
Nucleolus | 0.0025 | 0.0012 | 0.0012 | 0.0008 | 0.0044 | 0.002 | 0.0021 | 0.0009 | 0.0008 | 0.0012 | 0.0006 | 0.0013 | 0.0011 | 0.0011 | 0.0008 | 0.0006 | 0.001 | 0.0013 |
Nucleus | 0.783 | 0.9412 | 0.9404 | 0.9423 | 0.8177 | 0.9914 | 0.8308 | 0.9677 | 0.9115 | 0.7261 | 0.9827 | 0.9827 | 0.9339 | 0.9866 | 0.9852 | 0.9923 | 0.9953 | 0.9908 |
Peroxisomes | 0.015 | 0 | 0.0101 | 0.0008 | 0.0006 | 0 | 0.0037 | 0 | 0.0009 | 0.0246 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 |
Punctate Nuclear | 0.0179 | 0.0004 | 0.0003 | 0.0007 | 0.0011 | 0.0002 | 0.0121 | 0.0005 | 0.0079 | 0.0101 | 0.0004 | 0.0048 | 0.0093 | 0.0011 | 0.0015 | 0.0002 | 0.0002 | 0.0003 |
Vacuole | 0.0093 | 0.0039 | 0.0038 | 0.0003 | 0.0229 | 0.0003 | 0.0021 | 0.0003 | 0.0005 | 0.0045 | 0.0002 | 0.0002 | 0.0008 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0005 |
Vacuole Periphery | 0.0023 | 0.0002 | 0.0003 | 0.0001 | 0.0083 | 0 | 0.0007 | 0.0001 | 0.0002 | 0.0026 | 0.0001 | 0.0001 | 0.001 | 0 | 0.0001 | 0 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 48.4603 | 69.9794 | 66.7642 | 64.5246 | 54.7757 | 80.7225 | 64.2982 | 61.021 | 73.0035 | 57.4073 |
Translational Efficiency | 1.6157 | 1.3543 | 1.2673 | 1.6634 | 1.2208 | 1.1054 | 1.264 | 1.376 | 1.197 | 1.2754 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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