Standard name | |
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Human Ortholog | |
Description | Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 1 |
Bud | 0 | 2 | 0 | 0 | 0 | 2 | 1 | – | 1 | 1 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 8 | 3 | 0 | 7 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 0 | 2 | 0 | 2 | 5 | 4 | – | 6 | 9 | 6 | 12 | 0 | 4 | 1 | 8 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 318 | 266 | 177 | 244 | 244 | 286 | 349 | – | 205 | 222 | 271 | 310 | 242 | 400 | 387 | 232 | 177 | 183 | 297 | 246 | 174 | 149 | 266 | 278 |
Endoplasmic Reticulum | 3 | 5 | 0 | 0 | 0 | 0 | 1 | – | 1 | 3 | 0 | 3 | 1 | 0 | 0 | 12 | 2 | 6 | 0 | 0 | 1 | 0 | 2 | 1 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 0 | 0 | 1 | 0 |
Golgi | 1 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1 | 0 | 0 | 1 | 1 | 23 | 9 | – | 46 | 55 | 64 | 94 | 1 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 1 | 2 | 3 | 1 | 0 | 7 | – | 8 | 9 | 9 | 14 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 3 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 2 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 | 0 |
Vac/Vac Membrane | 3 | 2 | 3 | 14 | 17 | 20 | 67 | – | 37 | 39 | 31 | 35 | 0 | 2 | 0 | 3 | 0 | 1 | 2 | 3 | 3 | 0 | 1 | 1 |
Unique Cell Count | 325 | 273 | 183 | 259 | 259 | 315 | 415 | 268 | 305 | 341 | 421 | 243 | 401 | 387 | 236 | 178 | 184 | 328 | 270 | 193 | 151 | 292 | 305 | |
Labelled Cell Count | 331 | 278 | 185 | 263 | 267 | 336 | 439 | 306 | 343 | 389 | 478 | 244 | 406 | 388 | 256 | 185 | 197 | 328 | 270 | 193 | 151 | 292 | 305 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.8 | 9.3 | 9.6 | 9.6 | 11.6 | 7.5 | 8.7 | – | 6.9 | 7.0 | 6.7 | 6.6 | 9.9 | 9.3 | 10.6 | 35.2 | 34.4 | 34.5 | 9.2 | 9.6 | 10.4 |
Std Deviation (1e-4) | 2.9 | 3.0 | 2.8 | 2.3 | 2.7 | 2.2 | 2.2 | – | 1.9 | 1.7 | 1.7 | 1.6 | 3.0 | 3.2 | 3.1 | 7.8 | 7.8 | 9.4 | 2.7 | 3.2 | 3.6 |
Intensity Change (Log2) | – | – | – | -0.0 | 0.27 | -0.35 | -0.15 | – | -0.47 | -0.46 | -0.52 | -0.53 | 0.05 | -0.05 | 0.15 | 1.87 | 1.84 | 1.84 | -0.07 | 0.0 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.2 | -1.2 | -2.5 | -4.4 | – | -5.9 | -6.6 | -5.3 | -6.6 | 2.3 | 3.1 | 3.6 | 1.1 | 1.9 | 1.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.7 | 0 | – | 5.9 | 6.1 | 6.3 | 7.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.0 | 2.5 | 2.4 | 5.1 | – | 4.5 | 4.3 | 3.3 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.2229 | 3.4063 | 2.6153 | 1.8747 | 1.8774 | 3.1658 | 3.721 | 7.4137 | 5.6846 | 5.3304 | 5.3666 | 6.0065 | 6.4128 | 7.0857 | 6.1715 | 7.4392 | 6.8744 | 7.1967 |
Actin | 0.0046 | 0.0003 | 0.0007 | 0.0006 | 0.0008 | 0.0035 | 0.0186 | 0.0012 | 0.0072 | 0.0004 | 0.0112 | 0.0015 | 0.022 | 0.0136 | 0.0504 | 0.0007 | 0.0388 | 0.0134 |
Bud | 0.0005 | 0.0008 | 0.0003 | 0.0002 | 0.0002 | 0.0007 | 0.0003 | 0.0012 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0005 | 0.0024 | 0.002 | 0.0004 | 0.0014 | 0.0007 |
Bud Neck | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0011 | 0.0008 | 0.0005 | 0.0002 | 0.0006 | 0.0003 | 0.0005 | 0.0016 | 0.0012 | 0.0003 | 0.0004 | 0.001 | 0.0011 | 0.001 |
Bud Periphery | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.001 | 0.0019 | 0.0003 | 0.001 | 0.0006 |
Bud Site | 0.0008 | 0.002 | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0011 | 0.0047 | 0.001 | 0.0002 | 0.0002 | 0.0001 | 0.0022 | 0.0048 | 0.0036 | 0.0004 | 0.0013 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.7563 | 0.914 | 0.8537 | 0.8015 | 0.7527 | 0.8909 | 0.839 | 0.9482 | 0.9158 | 0.9351 | 0.9038 | 0.9463 | 0.6862 | 0.828 | 0.69 | 0.7546 | 0.6613 | 0.857 |
Cytoplasmic Foci | 0.0159 | 0.0044 | 0.0068 | 0.0098 | 0.0079 | 0.0103 | 0.0268 | 0.0047 | 0.0072 | 0.0119 | 0.0083 | 0.0123 | 0.014 | 0.0055 | 0.0198 | 0.0132 | 0.0111 | 0.0054 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0.0002 | 0.0005 | 0 | 0.0005 | 0.0001 |
Endoplasmic Reticulum | 0.0065 | 0.0009 | 0.003 | 0.0083 | 0.0054 | 0.0017 | 0.0075 | 0.001 | 0.0025 | 0.0042 | 0.0046 | 0.0035 | 0.0058 | 0.0026 | 0.0034 | 0.0042 | 0.0025 | 0.0018 |
Endosome | 0.0081 | 0.0007 | 0.0042 | 0.0063 | 0.0108 | 0.0034 | 0.0058 | 0.0054 | 0.0032 | 0.0327 | 0.0141 | 0.0031 | 0.0178 | 0.0026 | 0.0198 | 0.0708 | 0.0081 | 0.0016 |
Golgi | 0.0015 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0018 | 0.0005 | 0.0013 | 0.0011 | 0.0347 | 0.0005 | 0.0047 | 0.0018 | 0.0062 | 0.0048 | 0.0044 | 0.0006 |
Lipid Particles | 0.0018 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0022 | 0.0001 | 0.0012 | 0.0005 | 0.0009 | 0.0001 | 0.0073 | 0.0006 | 0.0052 | 0.002 | 0.027 | 0.0003 |
Mitochondria | 0.0038 | 0.0001 | 0.0003 | 0.0003 | 0.0009 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0033 | 0.0003 | 0.0017 | 0.0008 | 0.0201 | 0.0035 | 0.0333 | 0.0003 |
None | 0.1782 | 0.0714 | 0.1195 | 0.1593 | 0.1641 | 0.0781 | 0.0785 | 0.0273 | 0.0501 | 0.0069 | 0.0099 | 0.0181 | 0.1805 | 0.1312 | 0.1445 | 0.1282 | 0.1182 | 0.113 |
Nuclear Periphery | 0.0082 | 0.0004 | 0.0025 | 0.0041 | 0.0256 | 0.001 | 0.0015 | 0.0005 | 0.0009 | 0.0009 | 0.0014 | 0.0011 | 0.0193 | 0.0007 | 0.0061 | 0.0026 | 0.0038 | 0.0005 |
Nucleolus | 0.0002 | 0 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0008 | 0.0001 | 0.0006 | 0.0002 | 0.0015 | 0 |
Nucleus | 0.0052 | 0.003 | 0.0042 | 0.0056 | 0.0214 | 0.0044 | 0.003 | 0.0019 | 0.0032 | 0.0022 | 0.003 | 0.0048 | 0.0141 | 0.0016 | 0.0038 | 0.0043 | 0.0205 | 0.0024 |
Peroxisomes | 0.0023 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0013 | 0.0023 | 0.0001 | 0.0029 | 0.0001 | 0.0004 | 0.0046 | 0.0044 | 0.0008 | 0.0095 | 0.0011 | 0.0276 | 0.0002 |
Punctate Nuclear | 0.0031 | 0.0003 | 0.0013 | 0.0019 | 0.0042 | 0.0015 | 0.0082 | 0.0009 | 0.0008 | 0.0003 | 0.0005 | 0.0009 | 0.015 | 0.0005 | 0.0054 | 0.0012 | 0.0351 | 0.0005 |
Vacuole | 0.0015 | 0.0007 | 0.0016 | 0.001 | 0.0024 | 0.0006 | 0.0016 | 0.0006 | 0.0008 | 0.0018 | 0.0026 | 0.0007 | 0.0011 | 0.0007 | 0.0055 | 0.005 | 0.0008 | 0.0003 |
Vacuole Periphery | 0.0006 | 0.0001 | 0.0004 | 0.0003 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0013 | 0.0013 | 0.0005 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.2162 | 33.0999 | 29.4738 | 25.8362 | 25.6038 | 27.1381 | 37.9295 | 33.1293 | 33.8004 | 29.0032 |
Translational Efficiency | 1.7462 | 1.2669 | 1.4164 | 1.4831 | 1.0553 | 2.5931 | 1.4347 | 2.1263 | 1.624 | 1.6173 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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