ORF | |
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Human Ortholog | |
Description | Protein with similarity to ABC transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 4 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 4 | 1 | 3 | 3 | 4 | 5 | 9 |
Bud | 1 | 2 | 0 | 12 | 5 | 7 | 12 | 14 | 10 | 20 | 5 | 3 | 3 | 1 | 1 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 2 | 5 | 7 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 14 | 12 | 5 | 4 | 0 | 2 | 3 | 0 | 4 | 0 | 3 | 4 | 6 | 7 | 8 | 5 | 4 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 3 | 0 | 6 | 6 | 3 | 9 | 8 | 3 | 3 | 2 | 4 | 5 | 8 | 7 | 14 | 10 | 3 | 0 | 1 | 0 | 3 | 0 | 0 |
Endoplasmic Reticulum | 2 | 3 | 3 | 4 | 0 | 3 | 4 | 2 | 1 | 1 | 1 | 2 | 0 | 2 | 2 | 3 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Endosome | 1 | 3 | 5 | 5 | 20 | 7 | 9 | 18 | 0 | 1 | 1 | 3 | 3 | 3 | 4 | 6 | 5 | 1 | 5 | 7 | 10 | 3 | 12 | 8 |
Golgi | 0 | 13 | 24 | 6 | 5 | 0 | 2 | 5 | 2 | 0 | 0 | 1 | 7 | 12 | 29 | 43 | 64 | 19 | 2 | 20 | 15 | 11 | 18 | 14 |
Mitochondria | 126 | 220 | 285 | 232 | 257 | 382 | 475 | 427 | 316 | 362 | 177 | 167 | 138 | 165 | 192 | 6 | 10 | 4 | 110 | 194 | 242 | 107 | 129 | 143 |
Nucleus | 2 | 1 | 1 | 1 | 0 | 4 | 5 | 5 | 2 | 1 | 1 | 2 | 1 | 0 | 1 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 10 | 14 | 16 | 9 | 16 | 45 | 61 | 58 | 25 | 37 | 23 | 14 | 7 | 5 | 5 | 6 | 1 | 0 | 0 | 1 | 1 | 1 | 2 | 1 |
Nucleolus | 12 | 4 | 5 | 6 | 10 | 32 | 44 | 42 | 25 | 29 | 23 | 13 | 2 | 1 | 4 | 4 | 4 | 1 | 0 | 0 | 0 | 1 | 2 | 2 |
Peroxisomes | 0 | 6 | 7 | 6 | 13 | 6 | 13 | 7 | 0 | 2 | 2 | 3 | 2 | 4 | 10 | 5 | 7 | 0 | 4 | 11 | 24 | 8 | 17 | 15 |
SpindlePole | 1 | 1 | 0 | 1 | 5 | 3 | 16 | 12 | 0 | 4 | 0 | 2 | 0 | 1 | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Vac/Vac Membrane | 15 | 32 | 20 | 2 | 25 | 26 | 33 | 21 | 9 | 11 | 10 | 7 | 13 | 18 | 20 | 15 | 26 | 9 | 2 | 9 | 2 | 1 | 3 | 9 |
Unique Cell Count | 140 | 248 | 302 | 252 | 298 | 419 | 540 | 494 | 328 | 384 | 187 | 187 | 150 | 189 | 227 | 97 | 109 | 37 | 133 | 258 | 304 | 146 | 199 | 220 |
Labelled Cell Count | 177 | 317 | 382 | 296 | 366 | 520 | 691 | 629 | 394 | 478 | 246 | 228 | 185 | 227 | 283 | 118 | 144 | 46 | 133 | 258 | 304 | 146 | 199 | 220 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.5 | 8.0 | 7.4 | 6.7 | 7.0 | 6.3 | 5.7 | 6.2 | 6.1 | 5.4 | 5.6 | 5.8 | 7.4 | 7.7 | 7.9 | 11.0 | 12.7 | 13.9 | 7.9 | 7.8 | 8.7 |
Std Deviation (1e-4) | 1.4 | 1.4 | 1.3 | 1.3 | 1.4 | 1.1 | 1.3 | 1.3 | 1.2 | 1.3 | 1.1 | 1.7 | 1.5 | 1.4 | 1.4 | 3.1 | 2.9 | 3.1 | 2.4 | 2.0 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.08 | -0.24 | -0.37 | -0.24 | -0.28 | -0.44 | -0.39 | -0.35 | 0.0 | 0.06 | 0.1 | 0.57 | 0.78 | 0.91 | 0.1 | 0.08 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 3.8 | 0 | 0 | 0 | 3.0 | 0 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.4 | -2.0 | -4.1 | -3.9 | -3.4 | -3.6 | -2.5 | 0 | -1.5 | -0.7 | -0.5 | -0.5 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 3.1 | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -2.9 | -3.6 | -5.9 | -6.1 | -5.1 | -4.6 | -5.6 | -4.0 | -3.6 | -1.3 | -0.7 | 1.8 | 8.3 | 11.1 | 0 |
Mitochondria | -1.1 | -3.4 | -1.6 | -3.0 | -3.5 | 1.2 | -0.1 | 0.1 | -2.1 | -1.0 | -2.8 | -3.7 | -17.0 | -16.9 | -13.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.0 | 0 | 2.6 | 2.9 | 3.0 | 1.2 | 2.1 | 2.8 | 1.0 | -0.3 | -1.4 | -1.8 | 0.3 | 0 | 0 |
Nucleolus | 0.6 | 1.3 | 3.6 | 3.9 | 4.0 | 3.5 | 3.5 | 4.9 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1.4 | -0.9 | 0.1 | -0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -3.5 | 0.8 | -0.2 | -0.3 | -1.5 | -2.3 | -2.4 | -0.6 | -1.4 | 0.8 | 1.2 | 0.9 | 2.7 | 4.9 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0542 | 0.0276 | 0.0221 | 0.0008 | 0.0011 | 0.007 | 0.0562 | 0.0095 | 0.0237 | 0.0016 | 0.0046 | 0.0032 | 0.1132 | 0.0766 | 0.0464 | 0.0007 | 0.0034 | 0.0254 |
Bud | 0.0004 | 0.0016 | 0.0061 | 0.0002 | 0.0002 | 0.0007 | 0.001 | 0.0005 | 0.0011 | 0.0002 | 0.0043 | 0.0002 | 0.0003 | 0.0005 | 0.0009 | 0.0001 | 0.0003 | 0.0001 |
Bud Neck | 0.0004 | 0.0004 | 0.0033 | 0.0001 | 0.0002 | 0.0009 | 0.0011 | 0.0003 | 0.0014 | 0 | 0.0003 | 0.0002 | 0.0004 | 0.0006 | 0.0009 | 0.0006 | 0.0001 | 0.0005 |
Bud Periphery | 0.0009 | 0.0018 | 0.0229 | 0.0005 | 0.0005 | 0.003 | 0.0018 | 0.0011 | 0.0014 | 0.0011 | 0.0273 | 0.0004 | 0.0004 | 0.0007 | 0.0031 | 0.0001 | 0.0012 | 0.0003 |
Bud Site | 0.0013 | 0.0195 | 0.0117 | 0.0002 | 0.0004 | 0.0004 | 0.0056 | 0.003 | 0.0344 | 0.0002 | 0.0009 | 0.0002 | 0.0008 | 0.0017 | 0.0033 | 0.0001 | 0.0002 | 0.0002 |
Cell Periphery | 0.0003 | 0.0003 | 0.0122 | 0.0001 | 0.0002 | 0.0005 | 0.0008 | 0.0004 | 0.0027 | 0.0003 | 0.0019 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0002 | 0.0002 | 0.0002 |
Cytoplasm | 0.0021 | 0.001 | 0.0013 | 0 | 0.0001 | 0.0003 | 0.0057 | 0.0003 | 0.0045 | 0.0011 | 0.0005 | 0.0018 | 0 | 0.0006 | 0.0007 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasmic Foci | 0.0054 | 0.0138 | 0.0034 | 0.001 | 0.0034 | 0.0031 | 0.0604 | 0.0239 | 0.0281 | 0.0027 | 0.0146 | 0.0163 | 0.0017 | 0.0093 | 0.0061 | 0.0272 | 0.0007 | 0.0038 |
Eisosomes | 0.0012 | 0.0005 | 0.0014 | 0.0001 | 0.0001 | 0.0003 | 0.0008 | 0.0005 | 0.001 | 0.0003 | 0.0003 | 0.0002 | 0.0017 | 0.0005 | 0.0015 | 0.0001 | 0.0003 | 0.0003 |
Endoplasmic Reticulum | 0.0035 | 0.0007 | 0.0006 | 0.0001 | 0.0002 | 0.0008 | 0.0457 | 0.0002 | 0.0003 | 0.0007 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 |
Endosome | 0.0168 | 0.024 | 0.0049 | 0.0089 | 0.0231 | 0.0202 | 0.1005 | 0.0088 | 0.0263 | 0.0154 | 0.0084 | 0.018 | 0.0036 | 0.0086 | 0.0082 | 0.0066 | 0.0119 | 0.0057 |
Golgi | 0.0167 | 0.0207 | 0.0101 | 0.008 | 0.022 | 0.0172 | 0.0596 | 0.0147 | 0.0191 | 0.0037 | 0.0161 | 0.032 | 0.027 | 0.0393 | 0.0308 | 0.0153 | 0.0168 | 0.0184 |
Lipid Particles | 0.0169 | 0.0384 | 0.011 | 0.0013 | 0.0302 | 0.0096 | 0.0567 | 0.0533 | 0.0136 | 0.0017 | 0.0625 | 0.0311 | 0.0033 | 0.0015 | 0.001 | 0.0508 | 0.0022 | 0.0086 |
Mitochondria | 0.8109 | 0.798 | 0.8642 | 0.9724 | 0.8947 | 0.9047 | 0.5136 | 0.8559 | 0.7794 | 0.959 | 0.7754 | 0.8441 | 0.8396 | 0.8331 | 0.8782 | 0.798 | 0.9544 | 0.9203 |
None | 0.0068 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0045 | 0.0001 | 0.0007 | 0.0018 | 0.0004 | 0.0112 | 0.0001 | 0.0001 | 0.0011 | 0 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0184 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0017 | 0.0304 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 |
Nucleolus | 0.0008 | 0.0008 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Nucleus | 0.0045 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0028 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Peroxisomes | 0.025 | 0.0453 | 0.0164 | 0.0025 | 0.0154 | 0.022 | 0.0349 | 0.0248 | 0.0529 | 0.0056 | 0.0777 | 0.0369 | 0.0065 | 0.0258 | 0.0143 | 0.0968 | 0.0011 | 0.013 |
Punctate Nuclear | 0.0062 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0095 | 0.0006 | 0.0002 | 0 | 0.0007 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 |
Vacuole | 0.0029 | 0.0026 | 0.0032 | 0.0012 | 0.0032 | 0.0026 | 0.0039 | 0.0008 | 0.0037 | 0.0023 | 0.0017 | 0.0008 | 0.0002 | 0.0002 | 0.0007 | 0.0011 | 0.0018 | 0.0008 |
Vacuole Periphery | 0.0043 | 0.0024 | 0.0037 | 0.0022 | 0.0042 | 0.0044 | 0.004 | 0.0007 | 0.0048 | 0.0018 | 0.0016 | 0.0013 | 0.0006 | 0.0004 | 0.0015 | 0.0018 | 0.0044 | 0.0019 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.9412 | 18.2662 | 18.5242 | 19.2755 | 12.8049 | 21.1028 | 27.0878 | 28.878 | 24.5089 | 23.8271 |
Translational Efficiency | 0.5351 | 0.8275 | 0.5444 | 0.6254 | 0.8241 | 0.5074 | 0.6944 | 0.5124 | 0.6439 | 0.6135 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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