Standard name
Human Ortholog
Description Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.1 0.07 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.93 0.98 0.97 0.92 0.88 – 0.78 0.74 0.64 0.72 1.0 0.99 0.99 0.89 0.84 0.9 0.9 0.97 0.94 0.93 0.92 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.07 0 0 0 0.19 0.12 – 0.24 0.34 0.49 0.37 0 0 0 0.07 0.15 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 1 0 1 – 1 0 3 4 0 0 0 0 0 1 0 0 0 0 0 2
Bud 2 0 1 0 0 4 1 – 4 4 11 7 1 0 0 0 0 0 0 0 0 1 3 0
Bud Neck 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 0 0 3 6 2 6 – 5 6 3 7 8 13 17 8 8 4 0 0 0 0 0 1
Cytoplasm 245 72 64 182 250 173 295 – 125 165 169 254 343 478 563 132 66 53 266 90 80 283 359 399
Endoplasmic Reticulum 0 0 2 0 2 0 1 – 2 1 0 6 1 1 3 9 5 1 11 0 1 4 8 7
Endosome 4 0 0 0 0 0 3 – 0 4 6 2 0 1 1 1 1 0 4 0 0 1 1 3
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 4 5 2
Mitochondria 11 5 1 7 1 36 39 – 39 76 129 129 0 2 0 10 12 1 5 1 0 5 5 6
Nucleus 2 0 2 1 0 2 1 – 4 2 5 3 0 0 0 5 3 2 0 0 0 1 0 0
Nuclear Periphery 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 2 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 2 – 0 1 1 2 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 10 0 0 1 2 0 14 – 3 4 9 10 0 4 0 7 4 4 3 1 0 1 1 3
Unique Cell Count 261 74 69 185 257 189 337 161 222 264 351 344 484 566 148 79 59 298 93 86 306 390 436
Labelled Cell Count 280 77 71 195 264 217 364 183 263 338 425 353 500 584 174 99 67 298 93 86 306 390 436


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.2 5.4 6.2 6.7 4.5 5.8 – 5.0 7.8 7.2 6.6 7.1 7.3 7.6 8.2 8.2 8.1 6.1 6.3 6.6
Std Deviation (1e-4) 0.9 0.6 0.4 1.3 1.5 1.4 1.5 – 1.1 14.0 15.7 9.3 1.0 1.2 1.3 1.9 2.4 1.8 1.1 1.5 1.3
Intensity Change (Log2) – – – 0.2 0.33 -0.25 0.1 – -0.12 0.54 0.43 0.28 0.39 0.44 0.49 0.61 0.6 0.6 0.18 0.22 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.3 1.8 -0.3 -1.2 – -2.7 -3.3 -4.6 -3.6 4.4 3.3 4.7 -0.8 -1.7 -0.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.6 – 4.2 5.4 7.2 5.8 0 0 0 0 2.9 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7418 0.0379 -0.1046 -0.4677 -0.4573 -0.2624 1.3469 2.0358 1.7497 1.601 1.6256 1.733 2.5942 3.6419 3.1853 3.0516 2.6771 3.259
Actin 0.0335 0.0028 0.0159 0.0004 0.0043 0.0005 0.0411 0.0024 0.0079 0.0046 0.0174 0.0114 0.0022 0.0003 0.0006 0.0009 0.0021 0.002
Bud 0.0005 0.0003 0.0006 0.0001 0.0031 0.0003 0.0016 0.015 0.0072 0.0066 0.0502 0.0012 0.0003 0.0011 0.0008 0.0014 0.0008 0.0001
Bud Neck 0.0032 0.0002 0.0004 0.0002 0.0029 0.0006 0.0024 0.0003 0.0014 0.0007 0.0007 0.0031 0.0041 0.0002 0.0005 0.0002 0.0003 0.0003
Bud Periphery 0.0005 0.0002 0.0004 0.0001 0.0022 0.0001 0.0011 0.0075 0.0041 0.0098 0.0332 0.0012 0.0005 0.0011 0.0024 0.0027 0.0008 0.0001
Bud Site 0.0028 0.0026 0.0032 0.0001 0.0164 0.0001 0.0166 0.0166 0.0194 0.0049 0.0067 0.0062 0.0019 0.0072 0.0021 0.0008 0.0005 0
Cell Periphery 0.0004 0.0005 0.0002 0.0001 0.0003 0.0001 0.0008 0.0006 0.0005 0.0004 0.0009 0.0001 0.0006 0.0003 0.0007 0.0002 0.0001 0.0001
Cytoplasm 0.5946 0.705 0.675 0.6376 0.7932 0.7693 0.5518 0.7636 0.7211 0.7106 0.5912 0.8136 0.5818 0.7231 0.6877 0.619 0.6818 0.7385
Cytoplasmic Foci 0.0258 0.0324 0.0247 0.0365 0.0254 0.0241 0.0766 0.011 0.017 0.0299 0.0307 0.0254 0.0166 0.0101 0.0155 0.022 0.0142 0.0068
Eisosomes 0.0002 0.0001 0.0001 0.0001 0.0001 0.0003 0.0004 0.0001 0.0003 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0107 0.0098 0.0076 0.0098 0.0062 0.0042 0.0112 0.0023 0.0034 0.0033 0.0026 0.0018 0.009 0.0029 0.0032 0.0032 0.0044 0.0033
Endosome 0.0499 0.0407 0.0535 0.0657 0.0296 0.0349 0.0341 0.0064 0.0332 0.0547 0.0216 0.0157 0.0109 0.0053 0.0115 0.0282 0.016 0.006
Golgi 0.0041 0.0026 0.0028 0.0011 0.0021 0.0015 0.0098 0.0005 0.0069 0.0063 0.0091 0.0056 0.0025 0.0004 0.0006 0.0017 0.0008 0.0004
Lipid Particles 0.0032 0.0013 0.0025 0.0009 0.0007 0.0005 0.0113 0.0002 0.003 0.0006 0.0135 0.0077 0.0033 0.0002 0.0004 0.0021 0.0003 0.0002
Mitochondria 0.0014 0.0008 0.0013 0.0003 0.0015 0.0008 0.0059 0.0036 0.0073 0.0203 0.0432 0.0038 0.003 0.0003 0.0003 0.0008 0.0003 0.0004
None 0.2429 0.1835 0.1941 0.2299 0.0972 0.154 0.1707 0.1641 0.1559 0.1246 0.1297 0.0871 0.3471 0.2426 0.2665 0.3077 0.2707 0.2368
Nuclear Periphery 0.0048 0.0016 0.0018 0.0022 0.0022 0.0008 0.0092 0.0005 0.0011 0.0015 0.001 0.0003 0.0009 0.0005 0.0006 0.0011 0.0006 0.0025
Nucleolus 0.0007 0.0002 0.0001 0.0001 0.0003 0.0001 0.0008 0.0002 0.0003 0.0003 0.0003 0 0.0002 0.0001 0.0001 0.0002 0.0001 0
Nucleus 0.005 0.0012 0.0011 0.0015 0.0033 0.001 0.0104 0.0011 0.0014 0.0018 0.0012 0.0005 0.0009 0.001 0.0008 0.001 0.0009 0.0005
Peroxisomes 0.001 0.0004 0.0003 0.0002 0.0007 0.0002 0.0239 0.0002 0.0019 0.0014 0.038 0.0127 0.0091 0.0001 0.0002 0.0004 0.0001 0.0001
Punctate Nuclear 0.0036 0.0011 0.0006 0.001 0.0014 0.0004 0.0133 0.0005 0.001 0.0018 0.0022 0.0005 0.0007 0.0005 0.0012 0.0005 0.0007 0.0003
Vacuole 0.0098 0.0114 0.0121 0.009 0.0061 0.0055 0.0054 0.0028 0.0047 0.0134 0.0052 0.0015 0.0036 0.0023 0.0037 0.0048 0.004 0.0012
Vacuole Periphery 0.0014 0.0013 0.0017 0.003 0.0011 0.001 0.0013 0.0005 0.001 0.0024 0.0011 0.0004 0.0004 0.0002 0.0004 0.0009 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.7028 10.2291 15.1808 14.6861 19.0748 11.4364 15.5139 12.9639 13.2738 12.5147
Translational Efficiency 0.3674 0.7964 0.509 0.5473 0.4081 0.9308 0.6157 0.6835 0.8006 0.8695

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Oca6

Oca6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Oca6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available