Standard name | |
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Human Ortholog | |
Description | Ubiquitin hydrolase; deubiquitinates intralumenal vesicle (ILVs) cargo proteins; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins destined for the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | – | 1 | 4 | 0 | 2 | 2 | 2 | 0 | 2 | 0 | 1 | 1 | 3 | 3 | 31 | 14 | 28 | 4 | 0 | 3 | 1 | 6 | 7 |
Bud | 2 | – | 4 | 15 | 12 | 21 | 41 | 64 | 33 | 53 | 29 | 34 | 2 | 10 | 26 | 3 | 1 | 7 | 0 | 0 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | – | 2 | 0 | 6 | 1 | 3 | 6 | 3 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 1 | 1 | 2 | 1 |
Bud Site | 0 | – | 0 | 0 | 0 | 0 | 0 | 8 | 5 | 9 | 5 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 63 | – | 83 | 42 | 29 | 31 | 78 | 77 | 52 | 78 | 35 | 55 | 67 | 106 | 189 | 213 | 124 | 212 | 21 | 6 | 47 | 55 | 40 | 27 |
Endoplasmic Reticulum | 13 | – | 17 | 15 | 2 | 11 | 6 | 1 | 2 | 3 | 2 | 4 | 2 | 7 | 6 | 24 | 10 | 28 | 2 | 1 | 1 | 1 | 3 | 4 |
Endosome | 107 | – | 0 | 15 | 81 | 22 | 61 | 63 | 14 | 16 | 7 | 15 | 61 | 144 | 66 | 120 | 104 | 165 | 166 | 13 | 71 | 44 | 151 | 221 |
Golgi | 2 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 6 | 0 | 0 | 0 | 2 | 5 |
Mitochondria | 6 | – | 35 | 79 | 80 | 151 | 164 | 182 | 173 | 265 | 186 | 249 | 2 | 1 | 24 | 7 | 6 | 6 | 26 | 4 | 26 | 8 | 22 | 27 |
Nucleus | 0 | – | 1 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 0 | – | 0 | 0 | 2 | 5 | 3 | 3 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Peroxisomes | 0 | – | 0 | 8 | 18 | 9 | 45 | 29 | 13 | 27 | 8 | 11 | 1 | 4 | 1 | 0 | 8 | 0 | 2 | 0 | 0 | 0 | 1 | 2 |
SpindlePole | 30 | – | 7 | 39 | 94 | 57 | 107 | 139 | 47 | 101 | 52 | 67 | 9 | 16 | 5 | 46 | 48 | 59 | 11 | 0 | 9 | 6 | 13 | 9 |
Vac/Vac Membrane | 3 | – | 0 | 1 | 7 | 4 | 8 | 4 | 3 | 3 | 5 | 7 | 6 | 6 | 10 | 15 | 23 | 20 | 9 | 3 | 16 | 16 | 24 | 28 |
Unique Cell Count | 199 | 135 | 170 | 265 | 255 | 395 | 442 | 267 | 418 | 263 | 357 | 138 | 262 | 294 | 396 | 272 | 444 | 258 | 31 | 184 | 140 | 283 | 347 | |
Labelled Cell Count | 235 | 150 | 218 | 333 | 315 | 520 | 581 | 347 | 562 | 331 | 452 | 152 | 301 | 331 | 465 | 341 | 530 | 258 | 31 | 184 | 140 | 283 | 347 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | – | 3.6 | 3.7 | 3.9 | 3.6 | 3.4 | 3.6 | 3.3 | 3.3 | 3.3 | 3.1 | 5.5 | 5.5 | 4.8 | 6.6 | 7.3 | 7.6 | 5.1 | 5.0 | 5.6 |
Std Deviation (1e-4) | 0.6 | – | 0.4 | 1.2 | 0.9 | 0.7 | 0.7 | 0.7 | 0.9 | 1.0 | 0.9 | 0.9 | 1.4 | 1.3 | 1.0 | 0.9 | 1.5 | 1.3 | 1.5 | 1.7 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.1 | -0.01 | -0.07 | -0.02 | -0.14 | -0.15 | -0.12 | -0.2 | 0.6 | 0.61 | 0.42 | 0.86 | 1.01 | 1.08 | 0.5 | 0.47 | 0.64 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 2.6 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -6.5 | -10.6 | -10.2 | -9.1 | -10.0 | -8.4 | -9.5 | -10.0 | -10.2 | -2.1 | -4.0 | 0.6 | -1.6 | -3.0 | -2.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.6 | -3.9 | 0 | -2.5 | -3.4 | -2.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8.8 | 10.8 | 0 | 7.3 | 8.3 | 8.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -5.9 | -8.4 | 0 | -9.0 | -7.5 | -9.7 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0.4 | 0 | 2.2 | 3.5 | 2.6 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.5571 | 1.6804 | 1.1872 | 1.1345 | 1.2107 | 1.2133 | 1.146 | 1.9058 | 1.6271 | 1.6328 | 1.557 | 1.5821 | -0.2337 | 0.287 | 0.2592 | 0.3331 | 0.152 | 0.1785 |
Actin | 0.0381 | 0.005 | 0.0085 | 0.0017 | 0.0289 | 0.0016 | 0.0229 | 0.0011 | 0.022 | 0.0134 | 0.03 | 0.0076 | 0.0044 | 0.0003 | 0.0045 | 0.0001 | 0.0074 | 0.0014 |
Bud | 0.0075 | 0.0082 | 0.0004 | 0.0002 | 0.0008 | 0.0007 | 0.0009 | 0.0001 | 0.0009 | 0.0003 | 0.0009 | 0.0003 | 0.0001 | 0.0003 | 0.0007 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0042 | 0.0004 | 0.001 | 0.0005 | 0.0017 | 0.0037 | 0.006 | 0.0002 | 0.0004 | 0.0002 | 0.0008 | 0.001 | 0.0003 | 0.0005 | 0.0004 | 0.0001 | 0.0006 | 0.0005 |
Bud Periphery | 0.0043 | 0.0047 | 0.0005 | 0.0002 | 0.0015 | 0.0012 | 0.0016 | 0.0001 | 0.0012 | 0.0002 | 0.002 | 0.0003 | 0.0001 | 0.0003 | 0.0028 | 0.0001 | 0.0001 | 0.0002 |
Bud Site | 0.0171 | 0.0161 | 0.0022 | 0.0006 | 0.0027 | 0.0007 | 0.0091 | 0.0006 | 0.0031 | 0.0004 | 0.0022 | 0.0004 | 0.0006 | 0.0046 | 0.0012 | 0.0004 | 0.0009 | 0.0001 |
Cell Periphery | 0.0016 | 0.0007 | 0.0005 | 0.0002 | 0.0005 | 0.0002 | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0001 | 0 | 0.0002 |
Cytoplasm | 0.1604 | 0.26 | 0.2311 | 0.1084 | 0.1901 | 0.2421 | 0.201 | 0.37 | 0.3298 | 0.1999 | 0.2764 | 0.2627 | 0.2206 | 0.1931 | 0.2055 | 0.1873 | 0.356 | 0.2292 |
Cytoplasmic Foci | 0.1957 | 0.2143 | 0.1907 | 0.2413 | 0.1752 | 0.2843 | 0.1752 | 0.1749 | 0.1783 | 0.1995 | 0.2203 | 0.2615 | 0.1585 | 0.2862 | 0.1464 | 0.2402 | 0.1202 | 0.2017 |
Eisosomes | 0.0014 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0116 | 0.0055 | 0.0085 | 0.0019 | 0.0069 | 0.0056 | 0.0076 | 0.0037 | 0.0051 | 0.0028 | 0.0031 | 0.006 | 0.0056 | 0.0032 | 0.0034 | 0.0038 | 0.0017 | 0.0038 |
Endosome | 0.2277 | 0.2934 | 0.2477 | 0.4636 | 0.3149 | 0.275 | 0.2439 | 0.2214 | 0.2715 | 0.3904 | 0.2719 | 0.3342 | 0.1823 | 0.1661 | 0.1333 | 0.3319 | 0.1749 | 0.2322 |
Golgi | 0.0264 | 0.0155 | 0.0223 | 0.0259 | 0.0492 | 0.0149 | 0.0177 | 0.0096 | 0.0168 | 0.0311 | 0.0338 | 0.0188 | 0.0074 | 0.0078 | 0.0065 | 0.0121 | 0.0048 | 0.0098 |
Lipid Particles | 0.0555 | 0.0251 | 0.0176 | 0.0123 | 0.0432 | 0.0297 | 0.0132 | 0.003 | 0.0117 | 0.0318 | 0.0629 | 0.0064 | 0.0229 | 0.0043 | 0.0113 | 0.0044 | 0.0109 | 0.0246 |
Mitochondria | 0.0368 | 0.0028 | 0.0157 | 0.0484 | 0.0559 | 0.006 | 0.023 | 0.0066 | 0.0087 | 0.0045 | 0.0399 | 0.0045 | 0.0015 | 0.001 | 0.0032 | 0.0017 | 0.0003 | 0.0076 |
None | 0.1511 | 0.1224 | 0.2269 | 0.0514 | 0.0919 | 0.0998 | 0.2345 | 0.196 | 0.1242 | 0.0976 | 0.0237 | 0.0759 | 0.3575 | 0.3134 | 0.4548 | 0.1991 | 0.2639 | 0.271 |
Nuclear Periphery | 0.0114 | 0.0011 | 0.0017 | 0.0015 | 0.0038 | 0.0019 | 0.0032 | 0.0006 | 0.0017 | 0.0017 | 0.0046 | 0.002 | 0.0022 | 0.0006 | 0.0009 | 0.002 | 0.001 | 0.0007 |
Nucleolus | 0.0013 | 0.0003 | 0.0003 | 0.0005 | 0.001 | 0.0002 | 0.0016 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0005 | 0.0003 | 0.0002 | 0.0002 | 0.0002 |
Nucleus | 0.0048 | 0.0012 | 0.0009 | 0.0013 | 0.0012 | 0.001 | 0.0017 | 0.0005 | 0.0009 | 0.0005 | 0.0008 | 0.0007 | 0.0011 | 0.0009 | 0.0007 | 0.0012 | 0.0004 | 0.0004 |
Peroxisomes | 0.0191 | 0.0048 | 0.0104 | 0.0027 | 0.0104 | 0.0175 | 0.021 | 0.0016 | 0.0122 | 0.0151 | 0.0147 | 0.0077 | 0.0198 | 0.0063 | 0.014 | 0.0035 | 0.0257 | 0.009 |
Punctate Nuclear | 0.0108 | 0.0018 | 0.0018 | 0.0009 | 0.0055 | 0.0028 | 0.0033 | 0.0005 | 0.0014 | 0.0009 | 0.0017 | 0.0012 | 0.0061 | 0.0019 | 0.003 | 0.001 | 0.0269 | 0.0012 |
Vacuole | 0.0103 | 0.0146 | 0.0091 | 0.0301 | 0.0106 | 0.0092 | 0.0092 | 0.0081 | 0.0077 | 0.007 | 0.0054 | 0.0066 | 0.0071 | 0.0074 | 0.0054 | 0.0088 | 0.0033 | 0.0048 |
Vacuole Periphery | 0.0027 | 0.002 | 0.0019 | 0.0062 | 0.0037 | 0.002 | 0.0022 | 0.001 | 0.0019 | 0.0024 | 0.0037 | 0.0019 | 0.0011 | 0.0008 | 0.001 | 0.002 | 0.0006 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.3321 | 7.803 | 5.2638 | 11.0502 | 6.6875 | 6.7413 | 10.669 | 13.0704 | 11.1816 | 12.3189 |
Translational Efficiency | 0.5244 | 0.4976 | 0.7183 | 0.3956 | 0.6557 | 0.9738 | 0.6503 | 0.4658 | 0.6307 | 0.5602 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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