Standard name | |
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Human Ortholog | |
Description | Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | – | 0 | 0 | 0 | 0 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud | – | 1 | – | 0 | 0 | 1 | 0 | – | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 3 | – | 0 | 3 | 2 | 5 | – | 3 | 14 | 1 | 11 | 0 | 1 | 2 | 4 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 116 | – | 138 | 250 | 131 | 250 | – | 221 | 242 | 122 | 221 | 219 | 335 | 369 | 146 | 145 | 115 | 39 | 112 | 31 | 181 | 272 | 281 |
Endoplasmic Reticulum | – | 0 | – | 0 | 1 | 1 | 2 | – | 5 | 6 | 3 | 20 | 0 | 0 | 0 | 1 | 3 | 7 | 0 | 0 | 0 | 1 | 2 | 0 |
Endosome | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Golgi | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 1 |
Mitochondria | – | 1 | – | 1 | 2 | 6 | 4 | – | 16 | 26 | 26 | 31 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 3 | 0 | 0 | 0 | 0 |
Nucleus | – | 1 | – | 2 | 1 | 4 | 1 | – | 12 | 6 | 3 | 8 | 1 | 0 | 0 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | – | 0 | – | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | – | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | – | 1 | – | 2 | 6 | 2 | 29 | – | 31 | 41 | 24 | 24 | 1 | 0 | 0 | 5 | 3 | 4 | 0 | 0 | 0 | 1 | 2 | 1 |
Unique Cell Count | 119 | 141 | 257 | 140 | 278 | 275 | 309 | 169 | 291 | 220 | 335 | 369 | 153 | 148 | 121 | 42 | 122 | 33 | 192 | 285 | 294 | |||
Labelled Cell Count | 123 | 143 | 264 | 147 | 291 | 291 | 337 | 181 | 317 | 221 | 336 | 371 | 161 | 160 | 135 | 42 | 122 | 33 | 192 | 285 | 294 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 8.6 | – | 9.2 | 10.7 | 7.9 | 10.1 | – | 9.6 | 9.0 | 9.0 | 9.4 | 11.2 | 11.2 | 11.2 | 12.0 | 12.7 | 12.9 | 9.2 | 10.0 | 9.8 |
Std Deviation (1e-4) | – | 1.7 | – | 1.6 | 1.7 | 1.9 | 1.7 | – | 1.9 | 1.7 | 2.2 | 2.0 | 1.8 | 1.8 | 1.8 | 2.9 | 3.8 | 3.5 | 1.9 | 2.2 | 2.0 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.3992 | 11.7552 | 10.4921 | 9.6433 | 9.5609 | 10.0194 | 11.3343 | 15.9582 | 14.6119 | 12.6929 | 12.6346 | 14.7022 | 12.8951 | 16.0757 | 14.8286 | 13.8623 | 13.0597 | 14.2052 |
Actin | 0.0057 | 0.0002 | 0.0062 | 0.0002 | 0.0182 | 0.0003 | 0.0337 | 0.0001 | 0.0062 | 0.0182 | 0.0513 | 0.0001 | 0.0072 | 0.0003 | 0.0003 | 0.0007 | 0.0165 | 0.018 |
Bud | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0004 | 0.0005 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 |
Bud Neck | 0.0008 | 0.0001 | 0.0004 | 0.0004 | 0.0013 | 0.0009 | 0.0018 | 0.0001 | 0.0004 | 0.0008 | 0.0014 | 0.0005 | 0.0005 | 0.0001 | 0.0002 | 0.0004 | 0.0012 | 0.0012 |
Bud Periphery | 0.0004 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0006 | 0 | 0 | 0.0007 | 0.0008 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0.0005 | 0.0001 |
Bud Site | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0.0041 | 0 | 0.0004 | 0.0026 | 0.0013 | 0 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0005 | 0.0004 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 |
Cytoplasm | 0.8671 | 0.9925 | 0.9784 | 0.9874 | 0.9218 | 0.9736 | 0.8485 | 0.996 | 0.9711 | 0.9122 | 0.8832 | 0.9862 | 0.9094 | 0.9833 | 0.9741 | 0.9476 | 0.89 | 0.9521 |
Cytoplasmic Foci | 0.0104 | 0.0009 | 0.0014 | 0.0012 | 0.0026 | 0.0027 | 0.0218 | 0.0004 | 0.0019 | 0.0072 | 0.0034 | 0.0007 | 0.0116 | 0.0005 | 0.0086 | 0.0096 | 0.0037 | 0.0033 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.001 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0055 | 0.0006 | 0.0027 | 0.0012 | 0.0086 | 0.0039 | 0.0061 | 0.0002 | 0.0015 | 0.006 | 0.002 | 0.0006 | 0.0086 | 0.0003 | 0.0003 | 0.0003 | 0.0014 | 0.0023 |
Endosome | 0.0354 | 0.0005 | 0.0013 | 0.0015 | 0.0175 | 0.0021 | 0.0146 | 0.0001 | 0.0011 | 0.0094 | 0.0188 | 0.0002 | 0.016 | 0.0001 | 0.0012 | 0.003 | 0.0099 | 0.0046 |
Golgi | 0.0041 | 0.0001 | 0.0004 | 0 | 0.0035 | 0.0001 | 0.0037 | 0 | 0.0002 | 0.0039 | 0.0054 | 0 | 0.0062 | 0 | 0.0007 | 0.0021 | 0.0277 | 0.0016 |
Lipid Particles | 0.0014 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0054 | 0 | 0.0003 | 0.0211 | 0.0074 | 0 | 0.0119 | 0 | 0.0081 | 0.0122 | 0.0056 | 0.0035 |
Mitochondria | 0.0071 | 0 | 0.0001 | 0.0001 | 0.0024 | 0.0001 | 0.0126 | 0 | 0.0085 | 0.0028 | 0.0009 | 0 | 0.0013 | 0.0121 | 0.0003 | 0.0004 | 0.0261 | 0.0014 |
None | 0.0067 | 0.0007 | 0.0009 | 0.0006 | 0.0008 | 0.0011 | 0.0088 | 0.0005 | 0.0011 | 0.0016 | 0.0015 | 0.0005 | 0.0043 | 0.0005 | 0.001 | 0.0005 | 0.0016 | 0.0009 |
Nuclear Periphery | 0.0177 | 0.0007 | 0.0014 | 0.0013 | 0.0104 | 0.0022 | 0.0105 | 0.0003 | 0.0008 | 0.0013 | 0.0068 | 0.0011 | 0.0054 | 0.0002 | 0.0004 | 0.0003 | 0.0011 | 0.0006 |
Nucleolus | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0 | 0.0009 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Nucleus | 0.0267 | 0.0033 | 0.0057 | 0.005 | 0.0063 | 0.0094 | 0.0135 | 0.0021 | 0.0046 | 0.0043 | 0.0051 | 0.0097 | 0.005 | 0.0018 | 0.0034 | 0.0025 | 0.0099 | 0.0061 |
Peroxisomes | 0.001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0045 | 0 | 0.001 | 0.0018 | 0.0048 | 0 | 0.0035 | 0 | 0.0009 | 0.0198 | 0.0018 | 0.0022 |
Punctate Nuclear | 0.0036 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0028 | 0.0055 | 0.0001 | 0.0002 | 0.0009 | 0.0039 | 0.0002 | 0.0062 | 0.0001 | 0.0003 | 0.0003 | 0.0005 | 0.0004 |
Vacuole | 0.003 | 0.0001 | 0.0004 | 0.0007 | 0.0024 | 0.0003 | 0.0018 | 0 | 0.0002 | 0.0017 | 0.0006 | 0.0001 | 0.001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0006 |
Vacuole Periphery | 0.0015 | 0 | 0.0001 | 0.0002 | 0.0009 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0005 | 0.0005 | 0 | 0.0004 | 0 | 0 | 0.0001 | 0.0004 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 108.2332 | 97.6101 | 99.116 | 138.2845 | 86.9296 | 74.6683 | 118.6054 | 108.0976 | 102.7869 | 128.8334 |
Translational Efficiency | 2.2945 | 2.3759 | 2.4106 | 1.3407 | 2.6279 | 3.8942 | 2.1326 | 1.7881 | 2.4297 | 2.2621 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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