Standard name
Human Ortholog
Description Catalytic subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form active complex, Psy4p may provide substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.83 0.85 0.93 0.79 0.94 0.89 0.88 0.84 0.73 0.79 0.66 0.7 0.82 0.93 0.98 0.79 0.69 0.7 0.8 0.78 0.85 0.76 0.62 0.53
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0.1 0.14 0 0 0 0 0 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.05 0.08 0.34 0.19 0.34 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0 0.08 0.09 0.11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0.08 0.1
Vac/Vac Membrane 0.21 0.19 0.13 0.27 0 0.11 0.1 0.13 0.08 0.08 0.12 0.11 0.24 0.11 0 0.25 0.41 0.28 0.05 0.05 0 0 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 4 2 1 3 2
Bud 0 1 0 0 0 0 0 1 3 1 5 3 0 0 0 0 0 0 5 2 1 1 15 16
Bud Neck 0 0 0 0 0 0 2 2 0 1 0 1 0 0 0 1 0 0 3 3 0 2 12 7
Bud Site 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 1 2 2 0 0 0 0 0 0
Cytoplasm 418 363 263 184 273 334 393 309 329 297 327 294 376 327 407 351 266 270 441 362 263 229 361 222
Endoplasmic Reticulum 2 1 3 0 0 0 1 0 0 2 0 1 0 0 0 32 18 13 0 0 0 1 5 5
Endosome 3 1 3 2 0 4 5 1 0 2 7 7 24 6 2 35 37 54 10 8 4 6 23 28
Golgi 1 0 1 0 0 0 0 0 0 0 0 0 0 1 1 0 1 2 3 0 2 0 5 2
Mitochondria 2 12 0 3 10 17 21 28 153 71 170 105 1 0 1 18 4 1 3 9 7 7 8 8
Nucleus 7 3 0 1 3 8 11 7 17 16 11 19 1 1 0 1 6 5 36 35 8 25 52 45
Nuclear Periphery 0 1 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 2 2
Nucleolus 3 0 0 0 1 0 0 0 1 1 0 1 1 0 0 5 3 5 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 3 5 5 0 0 0 0 2 0
SpindlePole 5 0 0 1 1 0 2 1 2 2 0 0 3 2 1 16 19 23 14 4 6 9 44 40
Vac/Vac Membrane 105 80 36 62 11 41 46 48 36 31 62 46 108 40 9 110 159 108 25 25 6 11 33 22
Unique Cell Count 503 425 284 232 290 377 445 369 448 377 497 423 458 351 416 445 387 387 553 464 310 303 587 419
Labelled Cell Count 546 462 306 253 299 404 481 397 544 425 582 481 516 377 421 573 520 488 553 464 310 303 587 419


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 5.1 6.5 6.2 4.9 4.9 4.9 5.1 3.9 4.4 4.2 4.4 6.6 6.3 5.6 5.5 6.7 7.3 6.6 6.8 6.9
Std Deviation (1e-4) 0.9 0.8 1.1 1.7 1.4 1.3 1.3 1.4 0.7 0.8 0.7 0.7 1.5 1.6 1.2 1.4 1.5 1.7 1.4 1.7 2.0
Intensity Change (Log2) – – – -0.06 -0.41 -0.41 -0.41 -0.35 -0.74 -0.56 -0.63 -0.57 0.03 -0.05 -0.21 -0.23 0.04 0.17 0.02 0.07 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -4.4 0.7 -1.7 -1.9 -3.4 -6.4 -4.9 -8.4 -7.4 -4.0 0.3 3.4 -5.0 -7.5 -7.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.8 2.6 1.9
Endosome 0 0 0 0 0 0 0 0 0 3.0 0 0 4.0 4.6 5.9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.6 3.7 4.7 0 0 0 0 0 0 0 3.4 0 0
Nucleus 0 0 0 0 0 3.3 3.5 0 3.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 3.2 3.8 4.2
Vacuole 4.0 -3.9 -0.7 -1.0 0.1 -2.1 -1.9 -0.1 -0.7 3.7 -0.5 -5.6 4.0 8.0 4.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9324 2.8335 2.4801 2.1356 2.0653 2.5344 7.3082 6.947 7.0277 7.2779 5.9692 6.7082 3.5735 4.355 4.2129 4.2713 4.3998 4.1659
Actin 0.0284 0.0005 0.0049 0.001 0.0686 0.0007 0.0287 0.001 0.0184 0.0007 0.0013 0.0026 0.0007 0.0011 0.0011 0.0008 0.0139 0.0006
Bud 0.0008 0.0002 0.0011 0.0002 0.0005 0.0006 0.0032 0.0013 0.003 0.0004 0.0005 0.0008 0.0001 0.0012 0.0005 0.0002 0.0004 0.0003
Bud Neck 0.0036 0.0004 0.0009 0.0009 0.0009 0.0048 0.0171 0.0006 0.0022 0.0008 0.001 0.0085 0.0007 0.0013 0.0011 0.0013 0.0011 0.005
Bud Periphery 0.0014 0.0001 0.0008 0.0001 0.0006 0.0004 0.0054 0.0005 0.0056 0.0007 0.0003 0.0015 0.0001 0.0011 0.0003 0.0001 0.0003 0.0002
Bud Site 0.0048 0.0016 0.0016 0.0003 0.0017 0.0006 0.01 0.0096 0.0108 0.0007 0.0002 0.0011 0.0006 0.0043 0.0016 0.0002 0.0008 0.0003
Cell Periphery 0.0004 0.0001 0.0002 0.0001 0.0001 0 0.0005 0.0001 0.0003 0.0016 0 0.0001 0 0.0001 0 0 0 0
Cytoplasm 0.3974 0.6484 0.5986 0.6088 0.6649 0.5116 0.3467 0.6232 0.5488 0.5736 0.7617 0.4263 0.3896 0.4858 0.4569 0.5198 0.495 0.3504
Cytoplasmic Foci 0.0387 0.0053 0.0103 0.0051 0.0161 0.0066 0.0437 0.0107 0.0098 0.0095 0.0088 0.0073 0.0083 0.006 0.0074 0.0057 0.0189 0.0066
Eisosomes 0.001 0 0.0001 0 0.0003 0 0.0002 0.0005 0.0002 0 0 0 0 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0101 0.0083 0.0087 0.0061 0.0055 0.0042 0.0096 0.0044 0.0074 0.0056 0.0052 0.0059 0.0027 0.0027 0.0025 0.0034 0.0046 0.0016
Endosome 0.017 0.0045 0.0099 0.0026 0.0093 0.0054 0.0367 0.0088 0.0079 0.0053 0.0047 0.0145 0.0058 0.0038 0.0039 0.0031 0.0077 0.0045
Golgi 0.0047 0.0002 0.0015 0.0001 0.0056 0.0002 0.0128 0.0026 0.005 0.0002 0.0005 0.0026 0.0002 0.0002 0.0001 0.0001 0.0043 0.0012
Lipid Particles 0.015 0.0003 0.0048 0.0003 0.0053 0.0002 0.0109 0.0004 0.0006 0.0004 0.0002 0.0014 0.0007 0.0001 0.0001 0.0001 0.0015 0.0005
Mitochondria 0.0099 0.0004 0.003 0.0005 0.0022 0.0006 0.0146 0.0015 0.0036 0.0007 0.0493 0.0031 0.0006 0.0008 0.0009 0.0007 0.0031 0.0008
None 0.2305 0.175 0.2083 0.2278 0.1607 0.1124 0.1422 0.1578 0.1777 0.2488 0.1137 0.0733 0.2406 0.1342 0.2315 0.2701 0.2979 0.142
Nuclear Periphery 0.0172 0.0089 0.0114 0.0056 0.0034 0.0082 0.0253 0.0079 0.013 0.0078 0.0104 0.0161 0.0084 0.0091 0.0085 0.009 0.0082 0.0053
Nucleolus 0.0021 0.0007 0.0009 0.0003 0.0002 0.0011 0.002 0.0008 0.0013 0.0006 0.0002 0.0028 0.0012 0.0014 0.001 0.0006 0.0011 0.002
Nucleus 0.175 0.136 0.1097 0.135 0.0325 0.3262 0.2298 0.1536 0.1549 0.1187 0.0312 0.3962 0.3122 0.3178 0.258 0.1664 0.1043 0.4454
Peroxisomes 0.029 0.0001 0.0054 0.0001 0.0161 0.0002 0.0243 0.0022 0.0019 0.0003 0.0002 0.0011 0.0008 0.0003 0.0007 0.0002 0.0161 0.0013
Punctate Nuclear 0.0088 0.0064 0.0152 0.0035 0.0042 0.0135 0.0239 0.0088 0.024 0.0176 0.0079 0.0303 0.0251 0.0262 0.022 0.0161 0.0196 0.0309
Vacuole 0.0027 0.0025 0.0023 0.0013 0.0012 0.0022 0.0096 0.0031 0.0031 0.0054 0.0021 0.0035 0.0015 0.0022 0.0015 0.0015 0.0011 0.0012
Vacuole Periphery 0.0014 0.0003 0.0006 0.0002 0.0003 0.0003 0.0028 0.0005 0.0007 0.0005 0.0006 0.0009 0.0002 0.0003 0.0003 0.0003 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.0447 11.7046 20.5289 13.4207 10.8029 9.5171 11.8345 8.3505 8.9073 14.808
Translational Efficiency 1.0522 1.165 0.7139 0.8966 1.2828 1.5645 1.6139 1.8809 1.2026 1.1087

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Catalytic subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form active complex, Psy4p may provide substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
Localization
Cell Percentages cytoplasm (56%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pph3

Pph3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pph3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available