Standard name
Human Ortholog
Description Chaperone that facilitates the assembly of cytochrome c oxidase; integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Bud 0 0 0.08 0.06 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.09 0.1 0 0 0 0 0 0
Cytoplasm 0.07 0.13 0.1 0.06 0.09 0 – 0 0 0 0 0 0 0.05 0.1 0.14 0.29 0.21 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0.08 0.11 0 0 0 0 0 0 0
Endosome 0 0.08 0 0 0.15 0 – 0 0 0 0 0 0 0.05 0.16 0.11 0.11 0 0.07 0.06 0.07 0.06 0.07 0.07
Golgi 0 0.07 0 0 0 0 – 0 0 0 0 0 0.21 0.17 0.12 0.35 0.17 0.26 0 0 0 0.05 0.12 0.07
Mitochondria 0.81 0.63 0.92 0.93 0.78 0.94 – 0.9 0.94 0.94 0.96 0.96 0.77 0.74 0.61 0.11 0.17 0.17 0.73 0.72 0.77 0.74 0.64 0.6
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.07 0 – 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0.05 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.16 0.28 0.05 0 0.12 0.08 – 0.06 0.06 0 0 0 0.15 0.15 0.12 0.19 0.23 0.28 0.07 0.07 0 0 0.05 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 7 2 1 0 0 0 – 0 0 0 0 0 3 3 0 2 1 4 8 7 1 1 5 8
Bud 7 1 23 15 6 13 – 32 19 11 9 17 1 1 1 1 0 2 3 2 0 0 0 3
Bud Neck 0 0 1 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 4 2 2 0 2 2
Bud Site 0 0 4 0 0 3 – 10 9 10 5 15 0 0 0 0 0 1 – – – – – –
Cell Periphery 11 9 3 4 2 2 – 4 6 2 2 3 4 5 5 2 3 7 0 0 1 0 1 0
Cytoplasm 29 31 26 16 23 15 – 30 14 12 10 9 7 9 13 5 10 15 6 4 2 0 1 2
Endoplasmic Reticulum 11 5 4 2 1 2 – 2 2 6 1 0 8 9 3 3 4 2 1 3 1 1 0 1
Endosome 16 18 1 1 39 4 – 8 1 1 0 0 7 9 20 4 4 3 32 18 18 6 15 17
Golgi 6 15 0 2 0 0 – 0 0 0 0 0 38 30 15 13 6 19 11 11 5 5 25 16
Mitochondria 329 146 249 252 200 383 – 834 518 489 352 445 141 134 78 4 6 12 337 215 208 77 143 148
Nucleus 4 3 2 1 4 12 – 22 7 3 7 12 0 0 0 0 0 1 1 1 0 0 1 1
Nuclear Periphery 1 3 0 0 2 12 – 15 21 25 12 17 4 0 0 0 0 0 4 1 1 0 1 0
Nucleolus 2 1 0 1 4 9 – 45 24 31 15 20 1 1 0 1 1 0 1 0 1 1 1 1
Peroxisomes 1 5 1 4 17 2 – 24 0 3 0 2 1 0 10 1 0 2 8 2 8 4 11 7
SpindlePole 3 4 7 2 8 8 – 27 10 8 4 14 1 5 0 0 1 1 3 2 1 1 1 0
Vac/Vac Membrane 65 65 14 5 32 33 – 57 33 16 16 15 28 27 15 7 8 20 30 21 11 2 10 31
Unique Cell Count 404 230 272 271 257 409 924 549 521 366 463 183 181 128 37 35 72 462 299 270 105 226 246
Labelled Cell Count 492 308 336 305 338 498 1110 664 617 433 569 245 233 160 43 44 89 462 299 270 105 226 246


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.4 4.1 4.5 5.3 4.4 – 4.5 4.0 4.2 4.2 4.0 6.5 6.5 6.2 7.8 8.4 7.9 6.0 6.5 6.9
Std Deviation (1e-4) 0.8 1.4 1.2 2.0 1.7 1.6 – 1.4 1.2 1.0 1.2 1.0 1.2 1.2 1.0 1.4 2.0 1.7 1.5 1.2 1.6
Intensity Change (Log2) – – – 0.14 0.39 0.11 – 0.13 -0.01 0.05 0.04 -0.02 0.69 0.68 0.61 0.94 1.05 0.96 0.57 0.68 0.76


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -3.1 0 – 0 0 0 0 0 -3.7 -3.7 -3.0 0 0 -1.7
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -0.2 -3.2 – -4.3 -4.4 -4.5 -3.7 -4.7 -2.3 -1.8 0.2 0 0 2.6
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 2.2 0 0 0 0
Endosome 0 6.4 0 – 0 0 0 0 0 0 0 6.4 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 7.9 6.9 0 0 0 0
Mitochondria 0 -4.4 0 – 0 0 0 0 0 -4.3 -5.1 -7.4 -12.0 -11.0 -13.2
Nucleus 0 0 2.0 – 1.7 0 0 0 1.8 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 4.0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.4 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.1 3.0 1.5 – 0.6 0.5 -1.5 -0.5 -1.3 3.7 3.6 2.4 0 0 5.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7206 2.0402 1.8023 1.9345 1.3275 1.6852 2.0537 1.5204 1.7902 1.552 1.9004 1.5531 2.8046 2.3531 2.2806 2.5106 1.6555 2.7666
Actin 0.0699 0.0131 0.0221 0.0183 0.07 0.0088 0.0524 0.0154 0.0294 0.0296 0.0563 0.0175 0.0457 0.0302 0.0347 0.0727 0.0309 0.0462
Bud 0.001 0.0025 0.0015 0.0162 0.0017 0.0006 0.0012 0.0069 0.0024 0.0059 0.0018 0.0015 0.0011 0.0035 0.0017 0.0105 0.0022 0.001
Bud Neck 0.0016 0.0029 0.0013 0.003 0.001 0.0067 0.0022 0.0012 0.0023 0.0006 0.0013 0.0161 0.0025 0.0015 0.0021 0.0011 0.0008 0.0024
Bud Periphery 0.0017 0.0035 0.0027 0.0367 0.0044 0.0011 0.0025 0.01 0.0049 0.0241 0.0025 0.0038 0.0033 0.0047 0.0033 0.0243 0.0044 0.0028
Bud Site 0.0018 0.0244 0.0061 0.018 0.0022 0.0015 0.006 0.0319 0.0121 0.0042 0.0038 0.0019 0.0026 0.0499 0.0222 0.0029 0.002 0.0024
Cell Periphery 0.0007 0.0018 0.0007 0.0013 0.0028 0.0005 0.0015 0.0014 0.0006 0.0008 0.0004 0.0006 0.0017 0.0028 0.0011 0.0014 0.0007 0.0007
Cytoplasm 0.024 0.0373 0.016 0.0221 0.0081 0.0065 0.0189 0.0624 0.0165 0.0202 0.0066 0.0167 0.0109 0.0345 0.0275 0.0308 0.0031 0.0066
Cytoplasmic Foci 0.0551 0.061 0.0757 0.0892 0.0711 0.0461 0.0678 0.0673 0.0603 0.0451 0.0446 0.0415 0.0476 0.0719 0.0405 0.0264 0.0159 0.0302
Eisosomes 0.0007 0.0005 0.0008 0.0002 0.0005 0.0003 0.0017 0.0004 0.0004 0.0004 0.0003 0.0004 0.0046 0.0015 0.0009 0.0006 0.0004 0.0006
Endoplasmic Reticulum 0.0097 0.0098 0.0024 0.0013 0.0032 0.0053 0.0235 0.005 0.0023 0.0152 0.0056 0.0021 0.0148 0.005 0.0037 0.0006 0.0024 0.0186
Endosome 0.1401 0.1628 0.1252 0.1022 0.138 0.1266 0.1961 0.1633 0.1329 0.1046 0.1497 0.1106 0.1047 0.1926 0.1117 0.027 0.098 0.1266
Golgi 0.0944 0.1219 0.1236 0.0339 0.0678 0.087 0.1014 0.1217 0.1017 0.077 0.1162 0.0937 0.0755 0.1157 0.088 0.0316 0.0999 0.13
Lipid Particles 0.0681 0.0259 0.0233 0.0275 0.011 0.0205 0.0659 0.0226 0.0202 0.0152 0.024 0.0415 0.049 0.0201 0.0136 0.0111 0.0022 0.0275
Mitochondria 0.411 0.4646 0.4999 0.5542 0.5413 0.6298 0.3466 0.4387 0.5633 0.6025 0.4835 0.5952 0.4848 0.3842 0.5749 0.716 0.7128 0.5508
None 0.0195 0.0126 0.017 0.0038 0.0013 0.0013 0.0085 0.0149 0.0144 0.0068 0.0746 0.0051 0.035 0.0223 0.0414 0.0006 0.0008 0.0025
Nuclear Periphery 0.0244 0.005 0.0084 0.0012 0.0012 0.0066 0.0255 0.0014 0.0013 0.002 0.0017 0.0024 0.0218 0.0031 0.0005 0.0002 0.0004 0.0141
Nucleolus 0.0011 0.0016 0.0008 0.001 0.0003 0.0003 0.001 0.0006 0.0004 0.001 0.0005 0.0054 0.0016 0.0009 0.0003 0.0001 0.0001 0.0016
Nucleus 0.0119 0.002 0.0012 0.0037 0.0005 0.0011 0.0067 0.001 0.0008 0.0013 0.0007 0.0022 0.0233 0.0062 0.0004 0.0002 0.0003 0.0034
Peroxisomes 0.0352 0.0227 0.0538 0.0286 0.056 0.0373 0.0427 0.0157 0.0179 0.0274 0.0165 0.0268 0.0495 0.0214 0.0175 0.0374 0.0107 0.0164
Punctate Nuclear 0.0072 0.0016 0.0041 0.0057 0.0009 0.0009 0.0063 0.0005 0.001 0.0009 0.002 0.0008 0.0079 0.0011 0.0004 0.0003 0.0001 0.0023
Vacuole 0.0119 0.0158 0.0075 0.0237 0.0094 0.0049 0.0119 0.0119 0.0089 0.0103 0.0038 0.0083 0.0065 0.0177 0.0072 0.0021 0.0055 0.0065
Vacuole Periphery 0.009 0.0068 0.006 0.0082 0.0073 0.0061 0.0096 0.006 0.006 0.0049 0.0037 0.0058 0.0056 0.0092 0.0064 0.0021 0.0065 0.0068

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.3456 17.6139 28.3189 20.2098 8.5155 26.257 23.0038 27.0453 27.1891 27.0616
Translational Efficiency 1.7887 1.3904 1.1753 1.9172 4.6763 1.6272 1.409 1.4294 1.6625 1.6534

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Chaperone that facilitates the assembly of cytochrome c oxidase; integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
Localization
Cell Percentages mitochondrion (95%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Pet100

Pet100


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pet100-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available