Standard name
Human Ortholog
Description Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.14 0.2 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0.1 0 0.08 0.06 0.29 0.38 0.31 0.38 0.55 0.54 0.49 0 0 0 0 0.08 0 0 0 0 0 0 0
Nucleus 0.46 0.53 0.34 0.35 0.48 0.42 0.39 0.39 0.41 0.41 0.38 0.37 0.42 0.35 0.41 0.41 0.41 0.48 0.28 0.48 0.18 0.47 0.43 0.24
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.68 0.64 0.71 0.67 0.52 0.47 0.48 0.44 0.38 0.37 0.39 0.37 0.59 0.53 0.41 0.53 0.56 0.46 0.67 0.42 0.69 0.41 0.36 0.42
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0.07 0.13 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 5 3 5 2 4 2 0 0 1 0 2 2 1 1 1 0 0 2
Bud Neck 0 0 0 0 0 0 0 1 0 0 1 1 1 0 0 0 0 0 0 0 1 0 0 1
Bud Site 0 0 0 0 2 4 6 16 12 14 15 15 0 0 1 0 1 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 2 7 5 12 7 17 10 9 5 10 11 16 32 60 6 7 10 0 0 0 1 0 1
Endoplasmic Reticulum 0 0 1 1 0 0 1 0 0 0 0 1 0 1 0 2 1 5 0 0 0 0 0 0
Endosome 0 0 5 0 4 4 3 26 7 3 2 7 1 6 7 3 10 2 0 0 8 1 2 6
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 1
Mitochondria 10 6 2 17 19 96 165 153 116 248 265 178 0 3 0 9 19 7 0 0 0 0 0 4
Nucleus 52 31 89 71 151 138 170 192 124 187 188 135 74 83 120 84 100 88 31 34 51 66 80 63
Nuclear Periphery 1 0 7 2 2 9 11 7 8 4 13 7 0 0 1 1 0 1 0 0 5 0 0 4
Nucleolus 76 38 182 137 163 156 208 217 115 169 193 134 105 125 121 110 135 83 76 30 195 59 66 110
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 4 0 3 9 7 22 10 18 8 15 1 9 12 3 4 3 1 1 6 3 4 9
Vac/Vac Membrane 0 0 4 3 8 5 4 6 9 7 6 11 2 2 1 9 15 8 2 2 7 10 24 52
Unique Cell Count 112 59 258 204 313 331 431 494 304 453 492 361 177 236 295 206 241 182 114 73 282 143 184 263
Labelled Cell Count 140 77 301 237 365 428 597 653 415 657 705 517 200 263 327 228 294 210 114 73 282 143 184 263


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 7.5 8.6 7.9 6.6 5.2 4.9 4.7 4.6 4.3 4.1 4.1 10.1 9.3 8.4 12.1 11.1 10.9 10.9 11.0 11.6
Std Deviation (1e-4) 1.1 1.4 1.8 1.6 1.5 1.3 1.7 1.4 1.2 1.2 1.3 0.9 2.6 2.4 2.2 3.2 3.4 3.4 2.8 2.5 2.3
Intensity Change (Log2) – – – -0.12 -0.39 -0.73 -0.81 -0.86 -0.91 -0.98 -1.06 -1.06 0.24 0.12 -0.02 0.5 0.38 0.35 0.34 0.37 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.2 0.7 -0.5 0.9 -0.6 0.2 0 -0.6 0.2 2.9 4.5 6.3 0.1 0.1 1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0.5 0.4 0 1.4 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.1 3.3 9.1 11.1 0 0 0 0 0 0 0 0 0 4.0 0
Nucleus 0.1 3.3 1.8 1.3 1.2 1.5 1.8 1.0 0.7 1.5 0.2 1.5 1.4 1.6 2.9
Nuclear Periphery 0 0 0 -0.1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.8 -4.5 -5.7 -5.7 0 0 0 0 0 -2.4 -4.0 -7.0 -3.8 -3.4 -5.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1.0 0 0 0 0 0 0 0 1.6 1.8 0 0 0
Vacuole 0 0.8 0 0 0 1.1 0 0 1.2 0 0 0 1.8 2.7 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7557 7.2604 6.1188 5.8291 4.2351 5.9494 5.4182 7.6292 7.2658 6.6197 5.8761 6.3023 4.7848 6.3059 6.261 3.761 4.5477 5.1206
Actin 0.0151 0.0018 0.0134 0.001 0.0949 0.0047 0.018 0 0.0002 0 0.0026 0 0.0215 0 0.0083 0.0152 0 0.0002
Bud 0.0005 0.0001 0.0002 0.0003 0.0005 0.0002 0.0004 0.0001 0.0001 0.0001 0.0004 0.0001 0.0004 0.0002 0.0004 0.0009 0 0.0001
Bud Neck 0.0036 0.0004 0.0008 0.0048 0.0006 0.0012 0.0005 0.0002 0.0001 0.0003 0.0003 0.0006 0.0006 0.0002 0.0004 0.0003 0.0003 0.0005
Bud Periphery 0.0007 0.0002 0.0003 0.0008 0.0016 0.0004 0.0006 0.0001 0.0001 0.0002 0.0005 0.0002 0.0008 0.0004 0.0007 0.0013 0.0001 0.0002
Bud Site 0.0041 0.0007 0.0057 0.0051 0.001 0.0011 0.0012 0.0003 0.0001 0.0002 0.0007 0.0002 0.001 0.0004 0.001 0.0027 0.0001 0.0001
Cell Periphery 0.0004 0.0002 0.0002 0.0018 0.0007 0.0002 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0004 0.0008 0.0002 0.0003 0.0001 0.0001
Cytoplasm 0.0086 0.0003 0.0089 0.0043 0.0008 0.0032 0.0023 0.0002 0.0001 0 0.0032 0 0.0051 0 0.002 0.0079 0 0.0005
Cytoplasmic Foci 0.0255 0.003 0.006 0.0113 0.0097 0.0042 0.0187 0.0029 0.0004 0 0.0048 0 0.0124 0.0001 0.004 0.0167 0.0001 0.0007
Eisosomes 0.0003 0 0.0002 0.0012 0.0022 0 0.0001 0 0 0 0 0 0.0005 0 0.0001 0.0003 0 0
Endoplasmic Reticulum 0.004 0.0001 0.0009 0.0007 0.022 0.001 0.0008 0 0.001 0 0.0032 0 0.0021 0 0.0012 0.0083 0 0.0002
Endosome 0.0264 0.0016 0.0047 0.0041 0.0132 0.0286 0.0079 0.0027 0.0137 0 0.0552 0 0.0083 0.0001 0.0084 0.1194 0 0.0075
Golgi 0.0057 0.0016 0.0042 0.0009 0.0229 0.009 0.0028 0.0004 0.0039 0 0.0044 0 0.0044 0 0.0015 0.0171 0 0.0039
Lipid Particles 0.014 0.0011 0.0046 0.0109 0.0099 0.0062 0.0081 0.0016 0.0003 0 0.0043 0 0.0127 0.0008 0.0019 0.0599 0.0003 0.008
Mitochondria 0.0034 0.0019 0.0052 0.0023 0.0843 0.0039 0.0033 0.0005 0.0036 0.0001 0.0071 0.0003 0.0038 0.0013 0.0099 0.0388 0.0005 0.0053
None 0.0104 0.0001 0.0062 0.0417 0.0015 0.0017 0.001 0.0001 0 0 0.0018 0 0.0126 0 0.0004 0.0032 0 0.0001
Nuclear Periphery 0.0103 0.0005 0.0014 0.0004 0.0029 0.0021 0.0107 0.0009 0.0065 0.0001 0.0575 0.0001 0.0171 0.0002 0.0119 0.0798 0.0005 0.0029
Nucleolus 0.4663 0.5559 0.6096 0.6317 0.3167 0.5989 0.5195 0.5779 0.5598 0.5636 0.4472 0.5081 0.5373 0.6022 0.6437 0.2092 0.6283 0.5977
Nucleus 0.3622 0.4235 0.3162 0.2635 0.3962 0.3239 0.3621 0.4088 0.4053 0.4347 0.3876 0.4894 0.3334 0.3865 0.2877 0.3724 0.3644 0.3571
Peroxisomes 0.0145 0.0034 0.0086 0.0046 0.0152 0.0012 0.0029 0.0004 0 0 0.001 0 0.0082 0.0001 0.0108 0.0039 0 0.0001
Punctate Nuclear 0.0187 0.0019 0.0019 0.0049 0.0009 0.0057 0.0328 0.0014 0.0002 0.0003 0.0064 0.0003 0.0128 0.0005 0.002 0.0183 0.0047 0.0112
Vacuole 0.0038 0.0013 0.0006 0.0033 0.0008 0.0017 0.0037 0.0009 0.0018 0.0003 0.004 0.0003 0.0023 0.0048 0.0017 0.0104 0.0002 0.0011
Vacuole Periphery 0.0016 0.0006 0.0003 0.0003 0.0015 0.0007 0.0023 0.0004 0.0025 0.0001 0.0075 0.0001 0.0021 0.0014 0.002 0.0139 0.0003 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 46.7941 35.1381 29.7988 24.3834 32.1554 26.1902 21.9936 21.4099 16.0931 26.5698
Translational Efficiency 2.0017 2.0354 2.2004 2.075 1.8313 1.8847 2.0754 1.3886 1.6417 1.9711

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1
Localization
Cell Percentages nucleolus (56%), nucleus (27%), mixed (16%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rrp8

Rrp8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rrp8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available