Standard name | |
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Human Ortholog | |
Description | Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF2 has a paralog, DNF1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 172 | 125 | 82 | 42 | 44 | 51 | 19 | 26 | – | 17 | 7 | 21 | 121 | 123 | 87 | 154 | 143 | 55 | 139 | 84 | 77 | 144 | 127 | 166 |
Bud | 56 | 39 | 29 | 15 | 23 | 33 | 35 | 30 | – | 31 | 14 | 32 | 51 | 51 | 39 | 50 | 72 | 22 | 127 | 85 | 34 | 177 | 147 | 167 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 70 | 36 | 25 | 121 | 80 | 97 |
Bud Site | 35 | 39 | 19 | 5 | 6 | 16 | 6 | 13 | – | 10 | 7 | 8 | 6 | 7 | 2 | 52 | 33 | 10 | – | – | – | – | – | – |
Cell Periphery | 57 | 31 | 37 | 12 | 15 | 12 | 11 | 16 | – | 16 | 18 | 14 | 70 | 70 | 79 | 70 | 52 | 39 | 49 | 12 | 27 | 21 | 43 | 61 |
Cytoplasm | 33 | 24 | 28 | 25 | 29 | 58 | 83 | 118 | – | 161 | 66 | 94 | 48 | 48 | 70 | 128 | 109 | 55 | 8 | 3 | 1 | 6 | 6 | 17 |
Endoplasmic Reticulum | 69 | 33 | 24 | 19 | 26 | 34 | 15 | 15 | – | 11 | 5 | 8 | 69 | 72 | 85 | 18 | 25 | 13 | 42 | 20 | 22 | 47 | 55 | 74 |
Endosome | 4 | 2 | 1 | 0 | 3 | 3 | 4 | 8 | – | 3 | 3 | 3 | 1 | 1 | 3 | 1 | 4 | 4 | 7 | 7 | 3 | 5 | 1 | 5 |
Golgi | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 3 | 3 | 2 | 3 | 1 | 1 | 4 | 2 | 2 | 2 | 0 | 3 |
Mitochondria | 22 | 41 | 6 | 18 | 14 | 60 | 67 | 59 | – | 98 | 43 | 91 | 0 | 0 | 2 | 44 | 30 | 10 | 11 | 12 | 9 | 21 | 20 | 24 |
Nucleus | 8 | 2 | 7 | 0 | 3 | 2 | 4 | 8 | – | 7 | 4 | 3 | 0 | 0 | 0 | 26 | 16 | 8 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | – | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 4 |
Nucleolus | 4 | 1 | 2 | 0 | 0 | 1 | 2 | 7 | – | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 13 | 5 | 8 | 4 | 8 | 9 | 2 | 2 | – | 3 | 0 | 3 | 3 | 3 | 2 | 3 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 5 | 1 | 0 | 1 | 2 | 2 | – | 1 | 2 | 1 | 6 | 4 | 5 | 19 | 19 | 8 | 1 | 0 | 0 | 1 | 0 | 1 |
Vac/Vac Membrane | 7 | 9 | 3 | 0 | 6 | 1 | 12 | 9 | – | 6 | 2 | 8 | 5 | 3 | 12 | 9 | 12 | 7 | 9 | 12 | 2 | 15 | 11 | 16 |
Unique Cell Count | 328 | 219 | 165 | 90 | 121 | 198 | 203 | 238 | 287 | 131 | 212 | 263 | 264 | 285 | 391 | 358 | 157 | 480 | 284 | 214 | 573 | 502 | 651 | |
Labelled Cell Count | 487 | 351 | 252 | 141 | 178 | 282 | 266 | 315 | 367 | 173 | 290 | 383 | 385 | 388 | 577 | 525 | 232 | 480 | 284 | 214 | 573 | 502 | 651 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.6 | 8.6 | 8.0 | 7.7 | 7.2 | 5.9 | 5.6 | 5.7 | – | 4.8 | 4.9 | 4.9 | 9.9 | 9.9 | 10.2 | 7.9 | 8.7 | 9.7 | 9.3 | 9.6 | 9.8 |
Std Deviation (1e-4) | 2.1 | 1.9 | 1.8 | 1.9 | 2.0 | 1.7 | 1.4 | 1.6 | – | 1.0 | 1.1 | 1.2 | 2.4 | 2.4 | 2.7 | 1.9 | 2.0 | 2.4 | 1.8 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.15 | -0.43 | -0.51 | -0.48 | – | -0.74 | -0.7 | -0.71 | 0.3 | 0.3 | 0.35 | -0.02 | 0.13 | 0.28 | 0.22 | 0.26 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.5 | -2.2 | -4.7 | -8.6 | -8.6 | – | 0 | -8.3 | 0 | -0.7 | -0.6 | -4.0 | -2.2 | -2.1 | -2.7 |
Bud | -0.2 | 0.3 | -0.2 | -0.1 | -1.4 | – | 0 | -1.7 | 0 | 0.5 | 0.5 | -1.1 | -1.5 | 0.7 | -0.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | -1.6 | -1.9 | -1.1 | -3.2 | -2.2 | – | 0 | -1.9 | 0 | -4.0 | -3.7 | -5.2 | 0.6 | -0.8 | -1.6 |
Cell Periphery | -1.8 | -2.2 | -4.5 | -4.8 | -4.6 | – | 0 | -1.9 | 0 | 1.0 | 1.0 | 1.2 | -1.2 | -2.2 | 0.5 |
Cytoplasm | 2.0 | 1.5 | 2.7 | 5.0 | 6.7 | – | 8.1 | 6.1 | 5.6 | 0.3 | 0.3 | 1.9 | 3.8 | 3.3 | 3.7 |
Endoplasmic Reticulum | 1.3 | 1.5 | 0.7 | -2.2 | -2.8 | – | 0 | -3.1 | 0 | 2.9 | 3.1 | 3.6 | -4.1 | -2.8 | -1.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.3 | 2.6 | 6.6 | 7.0 | 5.7 | – | 0 | 6.7 | 0 | 0 | 0 | 0 | 2.9 | 2.0 | 1.1 |
Nucleus | 0 | 0 | 0 | 0 | -0.5 | – | -1.1 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0.1 | 0.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0.6 | -0.1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 | 1.2 | 0.9 |
Vacuole | 0 | 0 | 0 | 2.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.7727 | 4.8054 | 5.4941 | 4.9185 | 5.7301 | 5.286 | 7.6532 | 7.9309 | 8.0043 | 7.9621 | 7.926 | 8.0684 | 7.7519 | 6.9226 | 7.8315 | 7.4037 | 7.4229 | 7.0822 |
Actin | 0.122 | 0.1329 | 0.119 | 0.1976 | 0.1887 | 0.1499 | 0.109 | 0.0579 | 0.0831 | 0.0452 | 0.2983 | 0.1043 | 0.0542 | 0.005 | 0.154 | 0.1094 | 0.4264 | 0.1349 |
Bud | 0.0348 | 0.0237 | 0.2632 | 0.0861 | 0.1183 | 0.0133 | 0.0303 | 0.0395 | 0.2158 | 0.0745 | 0.0497 | 0.0105 | 0.0019 | 0.0056 | 0.2339 | 0.1032 | 0.1115 | 0.0055 |
Bud Neck | 0.0747 | 0.0208 | 0.0115 | 0.0512 | 0.0117 | 0.4831 | 0.0521 | 0.009 | 0.0127 | 0.0094 | 0.0641 | 0.5914 | 0.1924 | 0.0156 | 0.0009 | 0.0009 | 0.002 | 0.5991 |
Bud Periphery | 0.1083 | 0.0326 | 0.3658 | 0.4338 | 0.4913 | 0.0498 | 0.0962 | 0.066 | 0.4527 | 0.733 | 0.293 | 0.0657 | 0.0103 | 0.0048 | 0.4732 | 0.4104 | 0.4125 | 0.0314 |
Bud Site | 0.0784 | 0.4204 | 0.0719 | 0.0142 | 0.0249 | 0.0189 | 0.2254 | 0.5626 | 0.1101 | 0.0161 | 0.0145 | 0.0349 | 0.0603 | 0.6443 | 0.0509 | 0.0358 | 0.0105 | 0.0086 |
Cell Periphery | 0.1059 | 0.0259 | 0.0105 | 0.035 | 0.0748 | 0.034 | 0.082 | 0.0288 | 0.0124 | 0.0727 | 0.0312 | 0.0599 | 0.0714 | 0.0105 | 0.0018 | 0.003 | 0.0112 | 0.0378 |
Cytoplasm | 0.1785 | 0.1852 | 0.0301 | 0.0392 | 0.0423 | 0.1092 | 0.1067 | 0.1536 | 0.0492 | 0.012 | 0.0275 | 0.0845 | 0.0151 | 0.1327 | 0.0266 | 0.008 | 0.0043 | 0.0793 |
Cytoplasmic Foci | 0.0224 | 0.0353 | 0.0268 | 0.0338 | 0.0015 | 0.0278 | 0.0515 | 0.0157 | 0.0154 | 0.0003 | 0.0284 | 0.0064 | 0.1076 | 0.0153 | 0.0033 | 0.0373 | 0.001 | 0.0272 |
Eisosomes | 0.0011 | 0.001 | 0.0003 | 0.0016 | 0.0003 | 0.0006 | 0.0019 | 0.0006 | 0.0003 | 0.0012 | 0.0012 | 0.0006 | 0.0045 | 0.0001 | 0.0003 | 0.0001 | 0.0007 | 0.0007 |
Endoplasmic Reticulum | 0.0891 | 0.0187 | 0.0174 | 0.0045 | 0.0232 | 0.0137 | 0.0115 | 0.0049 | 0.001 | 0.0073 | 0.0059 | 0.0049 | 0.0109 | 0.0043 | 0.0166 | 0.0033 | 0.0024 | 0.0097 |
Endosome | 0.0295 | 0.0358 | 0.0161 | 0.0345 | 0.0013 | 0.0298 | 0.0703 | 0.0188 | 0.0087 | 0.0006 | 0.0366 | 0.0045 | 0.2738 | 0.1065 | 0.0099 | 0.1147 | 0.002 | 0.0212 |
Golgi | 0.0216 | 0.0417 | 0.0238 | 0.0485 | 0.0024 | 0.0381 | 0.0502 | 0.0165 | 0.0107 | 0.0006 | 0.0524 | 0.0066 | 0.0312 | 0.0202 | 0.0081 | 0.0324 | 0.0069 | 0.0206 |
Lipid Particles | 0.0309 | 0.006 | 0.0048 | 0.0047 | 0.0006 | 0.0081 | 0.0303 | 0.0017 | 0.0017 | 0.0002 | 0.0143 | 0.0043 | 0.0821 | 0.0052 | 0.0008 | 0.0487 | 0.0005 | 0.0125 |
Mitochondria | 0.0029 | 0.0025 | 0.014 | 0.0032 | 0.0024 | 0.0065 | 0.0157 | 0.0032 | 0.0022 | 0.0012 | 0.0642 | 0.0015 | 0.0136 | 0.0015 | 0.0016 | 0.0105 | 0.003 | 0.0018 |
None | 0.002 | 0.002 | 0.0007 | 0.0004 | 0.0005 | 0.0006 | 0.0033 | 0.0106 | 0.0007 | 0.0006 | 0.0009 | 0.0064 | 0.0074 | 0.0004 | 0.0007 | 0.0028 | 0.0004 | 0.0005 |
Nuclear Periphery | 0.0046 | 0.0012 | 0.0019 | 0.0007 | 0.0029 | 0.0014 | 0.007 | 0.0007 | 0.0006 | 0.0014 | 0.0006 | 0.0006 | 0.0089 | 0.0012 | 0.0079 | 0.0265 | 0.0013 | 0.0004 |
Nucleolus | 0.001 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0027 | 0.0008 | 0.0005 | 0.0002 | 0.0005 | 0.0007 | 0.0027 | 0.0016 | 0.0001 | 0.0008 | 0.0001 | 0.0003 |
Nucleus | 0.0033 | 0.0011 | 0.001 | 0.0005 | 0.0009 | 0.0014 | 0.0026 | 0.0011 | 0.0011 | 0.0011 | 0.0007 | 0.0013 | 0.0025 | 0.0011 | 0.0038 | 0.0024 | 0.0005 | 0.0005 |
Peroxisomes | 0.001 | 0.0016 | 0.0064 | 0.0046 | 0.0003 | 0.0047 | 0.0283 | 0.0007 | 0.0056 | 0.0001 | 0.0065 | 0.004 | 0.0054 | 0.0016 | 0.0012 | 0.0224 | 0.0004 | 0.0038 |
Punctate Nuclear | 0.0534 | 0.0007 | 0.0008 | 0.0008 | 0.0001 | 0.001 | 0.0018 | 0.0004 | 0.0017 | 0.0001 | 0.0005 | 0.0021 | 0.0202 | 0.0004 | 0.0005 | 0.0216 | 0.0002 | 0.0008 |
Vacuole | 0.0314 | 0.0095 | 0.0113 | 0.004 | 0.0106 | 0.0066 | 0.0159 | 0.0063 | 0.012 | 0.0212 | 0.008 | 0.0045 | 0.016 | 0.0198 | 0.0031 | 0.0031 | 0.0019 | 0.0029 |
Vacuole Periphery | 0.0032 | 0.0007 | 0.0022 | 0.0009 | 0.0007 | 0.0011 | 0.0054 | 0.0006 | 0.0015 | 0.0011 | 0.0011 | 0.0003 | 0.0075 | 0.0023 | 0.0006 | 0.0027 | 0.0006 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 57.1198 | 41.3793 | 41.142 | 41.5761 | 47.1549 | 55.379 | 56.0595 | 50.0776 | 55.8745 | 56.4925 |
Translational Efficiency | 0.6319 | 0.5411 | 0.4586 | 0.5108 | 0.435 | 0.6202 | 0.4381 | 0.3808 | 0.3474 | 0.4443 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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