Standard name | |
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Human Ortholog | |
Description | Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 14 | 35 | 48 |
Bud | 0 | – | 5 | 3 | 3 | 3 | 5 | 7 | 15 | 14 | 9 | 13 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 10 | 36 | 56 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 23 | 27 |
Bud Site | 0 | – | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | – | 0 | 0 | 2 | 4 | 3 | 3 | 1 | 2 | 2 | 5 | 1 | 2 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 3 | 3 |
Cytoplasm | 47 | – | 144 | 300 | 626 | 499 | 825 | 886 | 586 | 670 | 334 | 567 | 136 | 268 | 290 | 286 | 168 | 216 | 56 | 22 | 142 | 30 | 69 | 152 |
Endoplasmic Reticulum | 0 | – | 1 | 2 | 1 | 2 | 0 | 3 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 16 | 10 | 8 | 2 | 0 | 1 | 0 | 0 | 2 |
Endosome | 0 | – | 0 | 0 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 4 | 4 | 2 | 0 | 1 | 3 | 5 | 5 |
Golgi | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 3 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 7 | – | 8 | 37 | 41 | 98 | 193 | 274 | 277 | 333 | 241 | 355 | 1 | 0 | 21 | 10 | 1 | 5 | 2 | 0 | 4 | 1 | 5 | 5 |
Nucleus | 0 | – | 0 | 6 | 2 | 1 | 3 | 3 | 8 | 3 | 2 | 4 | 2 | 3 | 4 | 11 | 9 | 6 | 0 | 3 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 6 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 1 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 2 | 4 |
Vac/Vac Membrane | 1 | – | 2 | 14 | 14 | 10 | 11 | 21 | 13 | 16 | 3 | 18 | 11 | 20 | 37 | 61 | 45 | 55 | 0 | 1 | 5 | 1 | 6 | 8 |
Unique Cell Count | 54 | 150 | 336 | 655 | 535 | 908 | 1027 | 722 | 825 | 467 | 760 | 145 | 289 | 330 | 329 | 205 | 267 | 74 | 29 | 165 | 74 | 193 | 320 | |
Labelled Cell Count | 56 | 161 | 363 | 694 | 620 | 1045 | 1204 | 904 | 1048 | 603 | 965 | 154 | 296 | 356 | 391 | 238 | 300 | 74 | 29 | 165 | 74 | 193 | 320 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | – | 4.0 | 4.0 | 4.0 | 3.7 | 3.3 | 3.5 | 3.0 | 3.0 | 3.0 | 2.8 | 5.8 | 5.8 | 4.9 | 6.0 | 6.6 | 7.3 | 5.7 | 5.5 | 6.1 |
Std Deviation (1e-4) | 0.7 | – | 1.2 | 0.5 | 0.7 | 0.9 | 0.7 | 0.8 | 0.8 | 0.7 | 0.8 | 0.6 | 1.1 | 1.1 | 0.9 | 1.4 | 1.5 | 1.7 | 1.6 | 1.3 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.01 | -0.13 | -0.27 | -0.2 | -0.42 | -0.42 | -0.4 | -0.52 | 0.53 | 0.53 | 0.3 | 0.6 | 0.72 | 0.88 | 0.52 | 0.46 | 0.6 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.4 | -0.2 | -1.2 | -2.1 | -3.4 | -4.5 | -4.5 | -6.2 | -5.8 | -0.9 | -1.4 | -2.8 | -3.0 | -4.0 | -4.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | -1.2 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 2.5 | 3.7 | 5.2 | 5.7 | 5.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7362 | 2.1789 | 1.9313 | 1.3635 | 1.5263 | 1.9685 | 2.9354 | 3.2269 | 3.0316 | 2.8847 | 2.776 | 2.848 | 0.4322 | 1.0616 | 0.9123 | 1.2357 | 0.4961 | 0.7833 |
Actin | 0.0355 | 0.002 | 0.0128 | 0.0238 | 0.0258 | 0.0116 | 0.048 | 0.002 | 0.0403 | 0.0007 | 0.0105 | 0.0025 | 0.019 | 0.0013 | 0.0147 | 0.0042 | 0.0074 | 0.0019 |
Bud | 0.0009 | 0.0008 | 0.0017 | 0.0014 | 0.0007 | 0.0007 | 0.0052 | 0.0043 | 0.0018 | 0.0002 | 0.0007 | 0.0017 | 0.0009 | 0.0005 | 0.0001 | 0.0045 | 0.0059 | 0.0034 |
Bud Neck | 0.0095 | 0.001 | 0.0091 | 0.002 | 0.0017 | 0.0093 | 0.0093 | 0.0005 | 0.002 | 0.0006 | 0.0011 | 0.0039 | 0.0083 | 0.0006 | 0.0002 | 0.0018 | 0.0004 | 0.0023 |
Bud Periphery | 0.0015 | 0.0006 | 0.0018 | 0.002 | 0.0023 | 0.0009 | 0.0069 | 0.0028 | 0.0018 | 0.0001 | 0.0007 | 0.0022 | 0.0014 | 0.0003 | 0.0001 | 0.0049 | 0.0097 | 0.0067 |
Bud Site | 0.0082 | 0.0097 | 0.0155 | 0.0016 | 0.0008 | 0.0005 | 0.0145 | 0.0055 | 0.0048 | 0.0002 | 0.0004 | 0.0002 | 0.0086 | 0.036 | 0.0003 | 0.0472 | 0.0047 | 0.0007 |
Cell Periphery | 0.0006 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0006 | 0.0009 | 0.0001 | 0.0007 | 0.0002 | 0.0003 |
Cytoplasm | 0.7254 | 0.7795 | 0.7468 | 0.7992 | 0.7835 | 0.8131 | 0.5552 | 0.8081 | 0.7692 | 0.79 | 0.651 | 0.7997 | 0.6123 | 0.6863 | 0.613 | 0.6273 | 0.6177 | 0.6806 |
Cytoplasmic Foci | 0.0144 | 0.0105 | 0.0144 | 0.0095 | 0.0104 | 0.0099 | 0.0226 | 0.0092 | 0.0106 | 0.0074 | 0.0176 | 0.0082 | 0.0105 | 0.0029 | 0.0089 | 0.0143 | 0.0091 | 0.0029 |
Eisosomes | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0117 | 0.0077 | 0.0074 | 0.0125 | 0.0098 | 0.0079 | 0.0054 | 0.0032 | 0.002 | 0.0065 | 0.0089 | 0.0035 | 0.0031 | 0.0032 | 0.003 | 0.0083 | 0.0028 | 0.0027 |
Endosome | 0.0197 | 0.0045 | 0.008 | 0.0049 | 0.0146 | 0.0065 | 0.0128 | 0.002 | 0.0117 | 0.021 | 0.0196 | 0.0072 | 0.0122 | 0.0008 | 0.0022 | 0.0025 | 0.0046 | 0.0022 |
Golgi | 0.0084 | 0.0004 | 0.0022 | 0.0031 | 0.0037 | 0.0008 | 0.0059 | 0.0006 | 0.0048 | 0.002 | 0.0124 | 0.0022 | 0.0032 | 0.0001 | 0.0017 | 0.0028 | 0.0033 | 0.0002 |
Lipid Particles | 0.0038 | 0.0009 | 0.0042 | 0.0034 | 0.0046 | 0.0008 | 0.0239 | 0.0001 | 0.0006 | 0.0053 | 0.0055 | 0.0021 | 0.0025 | 0.0002 | 0.0043 | 0.0041 | 0.026 | 0.0001 |
Mitochondria | 0.0057 | 0.0003 | 0.0072 | 0.0104 | 0.0105 | 0.0009 | 0.0157 | 0.0011 | 0.0055 | 0.0007 | 0.0215 | 0.0151 | 0.0012 | 0.0001 | 0.0007 | 0.0025 | 0.0097 | 0.0003 |
None | 0.084 | 0.145 | 0.1305 | 0.083 | 0.0518 | 0.0616 | 0.1859 | 0.1153 | 0.1323 | 0.1419 | 0.2112 | 0.0409 | 0.2886 | 0.262 | 0.3389 | 0.213 | 0.2763 | 0.2854 |
Nuclear Periphery | 0.0107 | 0.0025 | 0.0055 | 0.0038 | 0.0089 | 0.0068 | 0.0234 | 0.0022 | 0.0009 | 0.0094 | 0.0039 | 0.0028 | 0.0023 | 0.0009 | 0.0007 | 0.0009 | 0.0017 | 0.001 |
Nucleolus | 0.0008 | 0.0004 | 0.0017 | 0.0002 | 0.0006 | 0.0006 | 0.0006 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0021 | 0.0001 | 0.0001 | 0.0014 | 0.0004 | 0.0001 |
Nucleus | 0.0447 | 0.0278 | 0.0147 | 0.0304 | 0.0515 | 0.0565 | 0.0149 | 0.0359 | 0.0054 | 0.0076 | 0.0142 | 0.102 | 0.0124 | 0.0022 | 0.0026 | 0.0024 | 0.0043 | 0.0073 |
Peroxisomes | 0.0032 | 0.0004 | 0.0071 | 0.0042 | 0.0014 | 0.0006 | 0.0316 | 0.0025 | 0.0029 | 0.0004 | 0.0151 | 0.0006 | 0.0018 | 0 | 0.007 | 0.0518 | 0.0126 | 0.0001 |
Punctate Nuclear | 0.0066 | 0.003 | 0.005 | 0.0021 | 0.0055 | 0.0079 | 0.0128 | 0.0035 | 0.0012 | 0.0032 | 0.0037 | 0.0031 | 0.003 | 0.0007 | 0.0007 | 0.0007 | 0.0014 | 0.0006 |
Vacuole | 0.0035 | 0.0025 | 0.0034 | 0.0016 | 0.0068 | 0.0026 | 0.0021 | 0.0007 | 0.0009 | 0.0019 | 0.0009 | 0.0015 | 0.0052 | 0.0008 | 0.0006 | 0.0042 | 0.0012 | 0.0011 |
Vacuole Periphery | 0.001 | 0.0002 | 0.0006 | 0.0003 | 0.0046 | 0.0004 | 0.0019 | 0.0001 | 0.0003 | 0.0008 | 0.0007 | 0.0005 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.4236 | 10.7448 | 13.5543 | 18.9335 | 13.7659 | 9.21 | 19.5554 | 18.2091 | 15.2463 | 20.9174 |
Translational Efficiency | 0.6005 | 0.8947 | 0.9019 | 0.6262 | 0.7544 | 1.2822 | 0.5924 | 0.6538 | 0.8665 | 0.6744 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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