Standard name
Human Ortholog
Description Component of transport protein particle (TRAPP) complex III; TRAPPIII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating endosome-Golgi traffic and required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Cytoplasm 0.86 0.93 0.94 0.92 0.9 0.79 0.8 0.8 0.69 0.7 0.58 0.68 0.97 0.98 0.98 0.84 0.95 0.83 0.63 0.75 0.69 0.53 0.52 0.59
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.08 0 0 0.1 0.16 0.11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.08 0 0 0.05 0.24 0.22 0.14 0.44 0.42 0.52 0.29 0 0 0 0 0 0 0.07 0.09 0.19 0.17 0.16 0.13
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.22 0 0.08 0 0.1 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 11 2 2 10 11 1 11 9 4 3 1 7 0 0 0 1 1 0 9 1 3 5 10 8
Bud 2 5 2 1 8 11 17 10 25 25 33 17 3 6 4 0 0 0 1 1 0 2 5 5
Bud Neck 0 0 3 1 6 3 2 3 2 0 2 1 0 0 0 0 0 0 9 2 8 6 8 9
Bud Site 0 0 0 0 0 0 0 1 2 2 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 27 15 11 7 5 7 4 8 7 8 4 6 6 7 2 0 0 0 1 1 2 3
Cytoplasm 249 246 371 316 441 441 575 487 376 403 302 302 421 561 614 75 53 15 209 221 319 132 214 236
Endoplasmic Reticulum 9 7 14 4 11 7 10 13 5 2 2 6 5 3 4 5 2 0 27 4 8 26 66 44
Endosome 9 0 0 1 0 5 5 7 4 3 0 8 1 3 2 5 0 0 17 3 4 3 5 4
Golgi 2 0 0 0 0 0 1 0 0 0 0 0 0 1 0 2 0 0 7 0 1 2 4 0
Mitochondria 22 20 0 14 25 133 160 83 238 245 271 129 8 6 2 1 0 0 23 27 86 42 66 51
Nucleus 0 0 0 0 1 0 5 7 5 4 4 8 0 0 0 0 1 1 0 1 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 1 0
SpindlePole 1 0 0 2 1 2 8 13 2 1 1 8 0 0 0 0 0 0 3 1 0 0 2 1
Vac/Vac Membrane 1 3 0 5 3 19 8 25 6 10 31 11 2 1 3 4 1 4 13 23 19 24 19 25
Unique Cell Count 288 265 395 342 488 556 722 608 544 577 524 447 432 572 625 89 56 18 330 294 462 251 414 399
Labelled Cell Count 308 283 419 369 518 629 807 666 673 707 657 508 444 588 635 100 60 20 330 294 462 251 414 399


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.1 6.1 5.6 6.0 4.7 5.0 5.3 4.3 4.2 4.1 4.8 5.9 5.9 6.1 9.5 9.4 10.1 5.3 5.6 5.8
Std Deviation (1e-4) 0.7 0.8 0.6 0.8 1.2 0.7 0.9 0.7 0.7 0.7 0.8 0.8 0.9 0.8 1.0 1.9 2.0 1.7 0.9 1.4 1.2
Intensity Change (Log2) – – – -0.13 -0.02 -0.38 -0.29 -0.22 -0.5 -0.53 -0.58 -0.33 -0.05 -0.05 0.0 0.63 0.63 0.73 -0.19 -0.11 -0.07


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.6 2.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2.3 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 -3.3 0 -5.9 -4.9 0 0 0 0 -4.5 -4.9 -5.2 0.3 0 0
Cytoplasm -0.8 -1.9 -6.3 -6.3 -6.1 -9.3 -9.2 -12.3 -9.5 2.5 3.4 3.7 -3.1 0.2 -1.8
Endoplasmic Reticulum -2.1 -1.1 0 -2.4 -1.3 0 0 0 0 -2.3 -3.5 -3.4 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.1 4.6 0 10.1 7.7 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2.9 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.7 0 4.1 0 0 4.9 3.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6082 0.8923 0.6501 0.4392 0.6353 0.8907 0.9901 1.4706 1.3807 1.2214 0.6736 1.2836 0.4729 1.0277 0.8078 0.5531 0.3469 0.8291
Actin 0.0225 0.0025 0.025 0.0031 0.0186 0.0099 0.0258 0.0021 0.002 0.0044 0.0097 0.0038 0.0168 0.0013 0.0136 0.0004 0.0321 0.004
Bud 0.0013 0.0015 0.0028 0.0086 0.0006 0.0004 0.0012 0.0007 0.0032 0.0017 0.0022 0.0018 0.0008 0.0004 0.0013 0.0003 0.0016 0.0003
Bud Neck 0.0131 0.0011 0.0018 0.001 0.0006 0.0036 0.002 0.0004 0.0005 0.0019 0.0013 0.0022 0.0064 0.0003 0.0018 0.0014 0.0009 0.0039
Bud Periphery 0.0019 0.0013 0.0027 0.0031 0.0006 0.0007 0.002 0.0003 0.0025 0.0009 0.0024 0.0014 0.0011 0.0002 0.0013 0.0002 0.0023 0.0003
Bud Site 0.021 0.015 0.0119 0.003 0.0008 0.0006 0.0057 0.0144 0.0043 0.0014 0.0114 0.0004 0.0102 0.0036 0.0072 0.0004 0.0023 0.0003
Cell Periphery 0.0015 0.0009 0.0016 0.0007 0.0002 0.0003 0.0009 0.0004 0.0003 0.0002 0.0003 0.0001 0.0016 0.0003 0.0006 0.0002 0.0004 0.0002
Cytoplasm 0.5696 0.6809 0.5766 0.5916 0.516 0.6904 0.6471 0.8094 0.7966 0.7833 0.739 0.7821 0.6725 0.7806 0.7459 0.7839 0.5534 0.8035
Cytoplasmic Foci 0.0537 0.0439 0.0695 0.0438 0.0531 0.0455 0.0649 0.0489 0.0333 0.0573 0.0545 0.0489 0.0675 0.0442 0.0487 0.0277 0.0923 0.0392
Eisosomes 0.0005 0.0002 0.0006 0.0002 0.0003 0.0002 0.0002 0 0 0 0.0001 0 0.0002 0.0002 0.0004 0 0.0003 0.0001
Endoplasmic Reticulum 0.0135 0.0065 0.0123 0.0067 0.0086 0.0121 0.0063 0.0032 0.0032 0.0032 0.0044 0.0047 0.018 0.0042 0.0087 0.0062 0.0093 0.0076
Endosome 0.0649 0.0465 0.1138 0.0936 0.0763 0.0754 0.0792 0.0423 0.0525 0.0655 0.0849 0.062 0.081 0.0567 0.0867 0.1434 0.1115 0.0681
Golgi 0.0081 0.0053 0.015 0.0043 0.0101 0.0094 0.0201 0.0042 0.0062 0.0249 0.0133 0.0058 0.0147 0.0068 0.0103 0.0027 0.0176 0.0064
Lipid Particles 0.0072 0.0007 0.0057 0.0046 0.0023 0.0054 0.0143 0.0006 0.0028 0.0005 0.0026 0.0062 0.0087 0.001 0.0016 0.0003 0.0039 0.0006
Mitochondria 0.012 0.0025 0.0141 0.0087 0.0061 0.0059 0.0083 0.0011 0.002 0.0047 0.0063 0.0058 0.0137 0.0016 0.0044 0.0006 0.0247 0.0026
None 0.1681 0.1753 0.1211 0.1783 0.2691 0.1178 0.091 0.0642 0.0757 0.0371 0.0368 0.064 0.0683 0.0908 0.0499 0.0127 0.1145 0.0538
Nuclear Periphery 0.0118 0.0017 0.005 0.0106 0.0215 0.0058 0.0054 0.0006 0.0006 0.001 0.0034 0.0006 0.0018 0.0007 0.0017 0.001 0.0021 0.0029
Nucleolus 0.0009 0.0002 0.0003 0.0058 0.0005 0.0003 0.0004 0.0001 0.0001 0.0001 0.0002 0.0001 0.0008 0.0001 0.0002 0 0.0003 0.0001
Nucleus 0.0049 0.0023 0.0023 0.0074 0.0034 0.0016 0.0019 0.001 0.001 0.001 0.0015 0.0008 0.0011 0.0009 0.0015 0.0008 0.0013 0.001
Peroxisomes 0.0068 0.0007 0.0016 0.0009 0.0006 0.0057 0.0133 0.0012 0.0039 0.0016 0.0185 0.0033 0.0042 0.0003 0.0014 0.0001 0.0123 0.0008
Punctate Nuclear 0.0081 0.0011 0.006 0.0028 0.0026 0.0017 0.0013 0.0006 0.0004 0.0008 0.0017 0.0005 0.0021 0.0004 0.0032 0.0001 0.0009 0.0007
Vacuole 0.0069 0.0087 0.0079 0.0165 0.005 0.0055 0.0076 0.0037 0.0076 0.0068 0.0043 0.004 0.0071 0.0046 0.0079 0.0132 0.0129 0.0023
Vacuole Periphery 0.0018 0.0013 0.0021 0.0046 0.0031 0.0017 0.0012 0.0007 0.0013 0.0017 0.0013 0.0013 0.0013 0.0008 0.0017 0.0043 0.0029 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.576 7.0556 6.9808 9.6673 12.621 9.466 11.8899 11.2348 12.2316 12.8962
Translational Efficiency 0.5916 0.6379 0.7309 0.453 0.4248 0.5528 0.5136 0.3892 0.4285 0.4791

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of transport protein particle (TRAPP) complex III; TRAPPIII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating endosome-Golgi traffic and required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
Localization
Cell Percentages cytoplasm (77%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-3

Trs85

Trs85


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trs85-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available