Standard name
Human Ortholog
Description Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to replication fork barrier (RFB), and rDNA repeat segregation; related to retroviral integrases
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.07 0 0.06 – 0.06 0.05 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0 0.16 0.17 0.06 0.05 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.07 0.07 0.08 0 0 0.05 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0 0 0 – 0.05 0 0.07 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.16 0.14 0.19 0.07 0.05 0.07 0.07 – 0.06 0.05 0.08 0.06 0.16 0 0.06 0.06 0.07 0 0.05 0.06 0.07 0 0.06 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.81 0.77 0.7 0.62 0.55 0.51 0.56 – 0.53 0.49 0.59 0.58 0.74 0.55 0.62 0.77 0.78 0.78 0.42 0.43 0.44 0.38 0.31 0.29
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.4 0.48 0.73 0.76 0.81 0.83 0.84 – 0.8 0.84 0.7 0.8 0.58 0.7 0.62 0.6 0.56 0.59 0.43 0.36 0.37 0.51 0.49 0.49
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0
Bud 1 0 0 0 1 1 5 – 2 7 5 5 0 0 0 0 1 1 2 1 0 0 0 1
Bud Neck 13 1 3 10 18 10 28 – 14 18 21 18 4 1 3 1 3 1 3 4 1 0 0 2
Bud Site 8 0 0 1 2 5 4 – 9 8 16 14 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 1 2 9 7 5 9 – 6 6 6 6 4 25 23 7 9 14 0 0 0 0 0 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 1 0 2 0 0 0 0 0 0
Endosome 6 9 5 4 9 7 14 – 4 6 2 6 3 8 6 8 14 13 12 8 7 0 4 7
Golgi 0 0 1 0 1 0 0 – 0 1 0 0 0 0 1 1 0 0 0 0 1 0 0 0
Mitochondria 26 2 0 0 0 6 1 – 12 5 22 13 1 0 0 6 8 4 0 1 1 0 1 1
Nucleus 48 24 24 18 14 21 34 – 14 17 24 23 17 7 8 7 14 2 17 12 10 0 5 2
Nuclear Periphery 1 1 1 0 0 0 2 – 0 0 0 0 1 0 1 1 2 2 2 2 0 0 0 0
Nucleolus 250 137 87 151 149 157 275 – 125 169 183 238 78 88 84 84 155 124 139 83 67 12 28 35
Peroxisomes 3 3 0 0 1 3 5 – 2 1 8 2 0 0 0 1 4 2 0 1 0 0 0 0
SpindlePole 122 85 90 185 220 257 412 – 188 289 217 332 61 111 85 65 112 93 140 70 56 16 45 59
Vac/Vac Membrane 0 2 0 1 1 1 0 – 0 1 0 0 0 1 1 0 2 3 3 0 1 0 2 2
Unique Cell Count 307 177 124 242 270 308 490 236 344 312 413 106 159 136 109 199 158 330 194 153 33 94 122
Labelled Cell Count 481 265 213 379 423 473 789 376 528 504 657 169 242 213 182 325 262 330 194 153 33 94 122


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 7.1 8.0 7.2 7.4 5.8 6.0 – 5.0 5.4 4.7 5.0 7.5 7.6 7.2 10.9 10.2 10.2 – 7.0 7.9
Std Deviation (1e-4) 1.3 1.5 1.3 1.5 1.7 1.3 1.3 – 1.4 1.2 1.0 1.1 1.4 1.8 1.7 3.2 2.6 2.4 – 1.7 1.5
Intensity Change (Log2) – – – -0.15 -0.12 -0.46 -0.41 – -0.69 -0.57 -0.78 -0.68 -0.09 -0.08 -0.16 0.45 0.35 0.34 – -0.19 -0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 1.7 0 1.5 – 1.5 1.3 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 4.0 4.2 0 0 2.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0.4 0.2 1.1 1.1 1.4
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus -3.4 -4.4 -3.9 -4.2 – -3.9 -4.9 -3.5 -4.8 -0.7 -4.0 -3.3 -2.9 -3.3 -5.2
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.5 -2.8 -3.6 -2.8 – -3.2 -4.0 0 -2.5 0.6 -2.5 -1.4 1.2 1.6 1.6
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.8 2.0 2.6 3.0 – 1.5 2.8 0 1.9 -2.4 -0.5 -1.7 -2.1 -2.9 -2.4
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1027 1.8492 1.8997 2.0291 1.5653 1.69 2.766 2.6522 2.388 1.6385 1.8621 2.4075 1.0755 0.4914 0.2469 0.3315 0.1879 0.3195
Actin 0.0143 0 0.0181 0.0117 0.0002 0.0047 0.0217 0 0.0089 0.0036 0 0.0009 0.0494 0.0001 0.0015 0.0028 0.1098 0.0004
Bud 0.0002 0.0006 0.0003 0.0016 0 0.0002 0.0003 0 0.0009 0.0013 0 0 0.0008 0.0001 0.0001 0.0003 0.0004 0.0007
Bud Neck 0.0009 0.0035 0.0012 0.0011 0.0006 0.0009 0.0018 0.0017 0.0014 0.0036 0.001 0.0009 0.0021 0.0013 0.0023 0.0197 0.005 0.0008
Bud Periphery 0.0002 0.0006 0.0004 0.0029 0.0001 0.0003 0.0004 0.0001 0.002 0.0021 0.0001 0 0.0013 0.0002 0.0001 0.0016 0.0006 0.0003
Bud Site 0.0025 0.0027 0.0015 0.0024 0.0001 0.0004 0.0028 0.0009 0.0098 0.0224 0.0002 0.0002 0.0024 0.0013 0.0011 0.0018 0.0014 0.0003
Cell Periphery 0.0002 0.0002 0.0001 0.0003 0.0001 0.0001 0.0003 0.0002 0.0004 0.0009 0.0001 0 0.0003 0.0002 0.0003 0.0029 0.0003 0.0001
Cytoplasm 0.005 0.0001 0.0066 0.0004 0.0001 0.0002 0.0051 0.0001 0.0052 0.0005 0.0001 0.0001 0.002 0 0.013 0.0007 0.0006 0.0049
Cytoplasmic Foci 0.0526 0.0082 0.0212 0.0505 0.0174 0.0201 0.0358 0.0168 0.0199 0.034 0.0142 0.0143 0.0389 0.0104 0.0143 0.0102 0.0191 0.0149
Eisosomes 0.0001 0 0.0001 0.0002 0 0 0.0002 0 0.0001 0.0002 0 0 0.0002 0 0 0.0025 0.0003 0
Endoplasmic Reticulum 0.0008 0 0.0015 0.0017 0.0002 0.0001 0.0019 0 0.0056 0.0006 0 0.0001 0.003 0 0.0009 0.001 0.0004 0.0001
Endosome 0.0072 0.0004 0.0057 0.014 0.0031 0.0077 0.0113 0.0008 0.0085 0.024 0.0009 0.0016 0.0384 0.0004 0.0017 0.0054 0.0149 0.0022
Golgi 0.0056 0.0006 0.0041 0.0109 0.0055 0.0097 0.0064 0.0007 0.002 0.0081 0.0009 0.0018 0.0187 0.0005 0.0018 0.0023 0.0177 0.0016
Lipid Particles 0.0995 0.0262 0.0488 0.1485 0.2171 0.0529 0.0671 0.0297 0.0361 0.1175 0.0363 0.035 0.0621 0.0149 0.0401 0.0343 0.1853 0.0317
Mitochondria 0.0033 0.0001 0.0027 0.0244 0.0015 0.0052 0.0028 0.0001 0.0096 0.0122 0.0002 0.0005 0.0092 0.0045 0.0006 0.0037 0.0199 0.0027
None 0.0084 0.0001 0.0046 0.0006 0.0001 0 0.0103 0 0.0021 0.0007 0.0001 0.0001 0.0016 0.0001 0.0012 0.0077 0.0007 0.0005
Nuclear Periphery 0.0021 0.0013 0.0016 0.016 0.0048 0.0003 0.005 0.001 0.004 0.0031 0.0009 0.0003 0.0038 0.0012 0.0004 0.0015 0.0009 0.0017
Nucleolus 0.4943 0.672 0.7009 0.519 0.344 0.4029 0.5777 0.6207 0.6952 0.374 0.4874 0.3796 0.5342 0.6728 0.6182 0.6923 0.2614 0.3042
Nucleus 0.0239 0.0418 0.032 0.0293 0.0205 0.0192 0.0294 0.0517 0.0297 0.017 0.0237 0.0177 0.0397 0.0597 0.0493 0.0651 0.0319 0.0222
Peroxisomes 0.007 0.0005 0.0032 0.0037 0.0039 0.0019 0.0093 0.0009 0.002 0.0062 0.0016 0.0058 0.0064 0.0006 0.0068 0.0026 0.0262 0.0048
Punctate Nuclear 0.2708 0.2405 0.1446 0.1564 0.3793 0.472 0.2082 0.2735 0.1548 0.3586 0.4321 0.5409 0.1809 0.2303 0.2452 0.1351 0.3007 0.6054
Vacuole 0.0007 0.0006 0.0005 0.0024 0.0004 0.0008 0.0016 0.0009 0.0014 0.0057 0.0002 0.0001 0.003 0.0007 0.0012 0.0055 0.0013 0.0003
Vacuole Periphery 0.0006 0.0001 0.0002 0.0021 0.0011 0.0005 0.0006 0.0001 0.0005 0.0036 0.0001 0 0.0015 0.0004 0.0002 0.0011 0.0012 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9196 9.858 12.9089 17.9497 14.7182 10.3731 13.0455 11.2781 15.1825 19.7611
Translational Efficiency 1.1941 1.0092 1.0113 0.7481 0.8988 1.2697 0.7032 0.8534 0.6948 0.8537

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to replication fork barrier (RFB), and rDNA repeat segregation; related to retroviral integrases
Localization
Cell Percentages nucleolus (33%), spindle pole (5%), mixed (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Fob1

Fob1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fob1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available