Standard name
Human Ortholog
Description Catalytically inactive alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p; ALT2 has a paralog, ALT1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.95 0.95 0.94 0.87 0.8 0.85 – 0.8 0.7 0.68 0.98 0.97 0.99 0.95 0.95 0.95 0.98 0.93 0.92 0.83 0.85 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.1 0.08 0.18 0.31 0.45 0.27 – 0.37 0.53 0.56 0 0 0 0 0 0 0 0 0 0 0 0.08
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud 0 0 0 0 1 5 7 7 – 10 15 20 1 0 1 0 1 0 0 0 0 0 4 9
Bud Neck 1 0 0 0 0 0 2 2 – 5 3 2 1 3 0 0 1 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 1 0 1 – 2 4 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 1 1 2 2 0 2 – 3 1 3 0 0 1 2 2 8 0 0 0 0 0 0
Cytoplasm 175 45 105 281 490 647 648 679 – 552 357 582 324 344 531 314 283 266 178 72 113 65 211 223
Endoplasmic Reticulum 0 0 0 0 0 1 2 0 – 0 0 2 2 0 1 14 14 13 0 0 2 1 1 3
Endosome 0 0 0 1 0 0 0 1 – 1 0 0 0 3 0 7 5 4 0 0 0 1 4 18
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 2 0 0 0 0 0 4
Mitochondria 2 2 11 23 92 228 365 213 – 256 270 477 0 0 1 10 8 2 2 1 1 2 4 25
Nucleus 1 2 1 1 3 3 6 5 – 1 2 3 2 0 3 0 2 1 0 0 0 0 2 2
Nuclear Periphery 0 1 0 0 1 0 4 0 – 0 3 2 0 0 1 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 3 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 2 12 – 13 9 15 0 1 0 2 6 1 0 0 0 0 3 12
Vac/Vac Membrane 0 0 0 5 7 8 3 2 – 6 1 1 6 10 9 8 10 14 0 0 2 2 8 10
Unique Cell Count 176 46 111 295 521 741 810 803 693 508 852 332 353 539 330 299 280 183 78 123 79 248 320
Labelled Cell Count 181 50 118 312 596 896 1039 927 849 665 1110 336 363 548 357 332 311 183 78 123 79 248 320


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 4.5 3.7 3.8 3.7 3.2 2.7 3.0 – 2.7 2.6 2.5 5.7 5.4 5.5 5.5 5.6 5.8 5.6 6.3 6.3
Std Deviation (1e-4) 0.6 0.6 0.4 0.8 1.1 0.8 1.0 1.0 – 0.8 1.2 0.7 1.1 0.8 1.1 0.9 1.0 1.0 1.1 1.5 1.7
Intensity Change (Log2) – – – 0.04 0.01 -0.18 -0.46 -0.29 – -0.46 -0.47 -0.53 0.64 0.57 0.57 0.58 0.61 0.67 0.6 0.78 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6914 2.7227 2.1013 1.5264 1.6846 2.0766 1.9342 3.5566 2.86 2.6263 2.6212 2.7881 1.1428 2.3097 1.7048 1.5978 2.035 1.2632
Actin 0.0005 0.0007 0.0006 0.0007 0.0014 0.0008 0.0415 0.0026 0.0004 0.0005 0.002 0.0118 0.0106 0.0003 0.0004 0.0159 0.0005 0.0001
Bud 0.0011 0.0013 0.0072 0.0003 0.008 0.0007 0.0002 0.0002 0.0001 0.0001 0.0005 0.0005 0.0003 0.0001 0.0004 0.0012 0.0001 0.0003
Bud Neck 0.0005 0.0007 0.0011 0.0007 0.0012 0.0023 0.0023 0.0025 0.0004 0.0005 0.0017 0.0055 0.0004 0.0002 0.0004 0.0005 0.0008 0.0013
Bud Periphery 0.0003 0.0003 0.0009 0.0002 0.0012 0.0002 0.0003 0.0001 0 0.0001 0.0002 0.0003 0.0002 0 0.0001 0.0004 0 0.0002
Bud Site 0.0014 0.0058 0.0018 0.0002 0.0021 0.0002 0.0006 0.0162 0.0005 0.0002 0.0005 0.0007 0.0057 0.0003 0.0003 0.0014 0.0001 0.0003
Cell Periphery 0.0001 0.0001 0.0001 0 0.0002 0 0.0002 0.0001 0 0 0 0.0001 0.0001 0 0 0 0 0.0001
Cytoplasm 0.6335 0.6906 0.6575 0.6807 0.6722 0.6353 0.6577 0.8034 0.7786 0.7261 0.7209 0.7917 0.7574 0.9129 0.8826 0.7799 0.7703 0.8638
Cytoplasmic Foci 0.0189 0.0076 0.0105 0.0251 0.0097 0.0182 0.0168 0.0051 0.0048 0.0048 0.0068 0.0086 0.0273 0.0044 0.0144 0.0439 0.0248 0.0031
Eisosomes 0 0 0 0 0 0 0.0006 0 0 0 0 0.0001 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0038 0.0036 0.0026 0.0038 0.0041 0.003 0.0094 0.0019 0.003 0.0024 0.0041 0.003 0.0072 0.0084 0.0028 0.0032 0.0065 0.0026
Endosome 0.0059 0.0048 0.0044 0.0163 0.0049 0.0059 0.0091 0.0015 0.0014 0.0025 0.0035 0.0073 0.0245 0.0119 0.0116 0.082 0.0832 0.0076
Golgi 0.0004 0.0002 0.0002 0.0005 0.0001 0.0002 0.0033 0.0002 0.0001 0.0001 0.0003 0.0011 0.0066 0.0003 0.0016 0.0238 0.0066 0.0002
Lipid Particles 0.0008 0.0001 0.0002 0.0002 0.0001 0.0002 0.0034 0.0001 0.0001 0.0002 0.0001 0.0011 0.0106 0.0003 0 0.0078 0.007 0.0001
Mitochondria 0.0006 0.0005 0.0009 0.0009 0.0006 0.0006 0.0012 0.0002 0.0002 0.0004 0.0003 0.0005 0.0113 0.0002 0.0008 0.005 0.0007 0.0004
None 0.2774 0.1684 0.204 0.155 0.2067 0.2124 0.2076 0.136 0.153 0.2121 0.2363 0.1216 0.1109 0.0244 0.0444 0.0047 0.0027 0.0185
Nuclear Periphery 0.0051 0.0052 0.0045 0.006 0.0053 0.0052 0.0062 0.0014 0.0031 0.0028 0.0024 0.0039 0.0025 0.0038 0.004 0.0025 0.0054 0.0045
Nucleolus 0.0006 0.0005 0.0004 0.0003 0.0006 0.0006 0.0003 0.0001 0.0001 0.0002 0.0001 0.0002 0.0004 0 0 0.0001 0 0.0006
Nucleus 0.0407 0.1014 0.0898 0.0792 0.0628 0.0929 0.029 0.0256 0.0475 0.0428 0.0166 0.0333 0.0091 0.0294 0.0333 0.0171 0.0869 0.0648
Peroxisomes 0.0004 0.0002 0.0004 0.0007 0.0002 0.0005 0.0042 0.0002 0.0001 0.0002 0.0002 0.0034 0.0076 0 0.0003 0.0072 0.0013 0.0001
Punctate Nuclear 0.0064 0.0065 0.0115 0.0273 0.0173 0.0195 0.004 0.0018 0.006 0.003 0.0019 0.0041 0.0026 0.0009 0.001 0.0016 0.0019 0.0037
Vacuole 0.0015 0.0015 0.0012 0.0014 0.0011 0.0012 0.0015 0.0007 0.0006 0.0009 0.0012 0.001 0.004 0.0017 0.0012 0.0011 0.0007 0.0234
Vacuole Periphery 0.0003 0.0002 0.0002 0.0003 0.0002 0.0003 0.0007 0.0001 0.0001 0.0002 0.0002 0.0003 0.0004 0.0004 0.0003 0.0007 0.0004 0.0044

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.287 27.1836 28.8163 20.5548 27.6923 18.0904 24.5405 16.5632 18.3292 22.6336
Translational Efficiency 0.9962 1.0859 0.8591 1.1514 1.1278 1.6217 0.9182 1.0367 0.8627 0.8711

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Catalytically inactive alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p; ALT2 has a paralog, ALT1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (50%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Alt2

Alt2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available