Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.07 0.1 0.09 0.2 0.14 0.12 – 0.13 0.15 0.12 0.16 0.15 0.19 0.24 0.17 0.2 0.19 0 0 0 0.07 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0.05 0 0.07 0 – 0 0 0 0 0 0 0 0.07 0.1 0.09 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0 0 0.08 0 0.21 0.2 – 0.39 0.35 0.64 0.49 0 0 0 0.09 0 0.06 0 0 0 0 0.05 0.05
Nucleus 0.83 0.78 0.81 0.72 0.54 0.48 0.64 – 0.59 0.55 0.44 0.45 0.8 0.78 0.68 0.57 0.56 0.53 0.76 0.68 0.63 0.63 0.61 0.53
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0 – 0 0 0 0 0 0 0 0 0.06 0 0.05 0.06 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.13 0.17 0.1 0.1 – 0.05 0.05 0 0.05 0 0 0 0 0.05 0 0 0 0.06 0 0.05 0.06
Vac/Vac Membrane 0.05 0.13 0.05 0 0.07 0.08 0.06 – 0.07 0.08 0.1 0.09 0 0 0 0.17 0.11 0.17 0.1 0.1 0.17 0.17 0.17 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 0 0 1 1 3 6 – 1 3 5 0 0 0 0 1 1 0 2 7 3 2 1 4
Bud Neck 0 0 2 3 3 2 4 – 2 4 0 3 0 0 0 0 0 0 0 1 2 1 0 4
Bud Site 0 0 0 3 0 4 7 – 4 7 10 3 0 0 0 0 0 1 – – – – – –
Cell Periphery 1 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 14 20 23 19 50 53 49 – 45 49 47 46 22 48 63 69 64 66 5 10 6 15 13 13
Endoplasmic Reticulum 0 1 0 1 0 0 0 – 1 0 0 0 1 0 2 16 11 18 0 0 1 0 1 3
Endosome 1 7 5 10 10 26 3 – 5 2 3 3 0 2 6 31 32 32 4 9 4 2 2 7
Golgi 1 0 0 0 0 0 1 – 0 0 0 0 0 0 0 1 0 2 0 2 1 1 0 0
Mitochondria 52 8 4 17 11 81 84 – 136 113 248 137 1 0 0 37 10 20 1 4 5 5 15 22
Nucleus 236 226 182 152 138 187 263 – 207 178 171 127 117 196 181 234 180 181 229 223 174 146 200 266
Nuclear Periphery 0 0 1 0 2 1 1 – 0 0 0 0 0 0 0 1 1 1 0 0 1 0 0 3
Nucleolus 3 6 2 5 10 25 17 – 13 4 14 3 0 0 4 12 19 9 14 18 10 5 14 24
Peroxisomes 0 1 0 0 0 0 0 – 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0
SpindlePole 3 8 10 27 42 40 40 – 17 16 7 13 2 6 12 9 15 15 7 12 15 5 15 28
Vac/Vac Membrane 13 37 11 8 18 30 26 – 26 25 39 26 6 7 11 70 36 58 30 33 47 40 54 103
Unique Cell Count 284 289 224 210 254 390 414 352 324 387 282 146 251 268 414 321 339 301 330 279 233 326 499
Labelled Cell Count 324 315 240 246 285 453 501 457 401 544 361 149 259 280 483 369 403 301 330 279 233 326 499


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.8 5.8 5.6 5.8 4.9 4.9 – 4.3 4.3 3.8 4.0 6.1 5.8 5.8 7.9 7.9 7.2 5.4 5.5 5.8
Std Deviation (1e-4) 0.7 0.8 1.0 1.4 1.7 1.4 1.1 – 1.0 0.8 0.7 0.7 0.9 0.9 1.0 1.8 1.7 1.6 1.0 1.1 1.2
Intensity Change (Log2) – – – -0.07 -0.0 -0.26 -0.26 – -0.45 -0.43 -0.61 -0.53 0.05 -0.0 0.0 0.44 0.43 0.3 -0.1 -0.07 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 2.9 1.2 0.6 – 0.9 1.7 0.7 2.0 1.4 2.7 3.9 2.2 3.0 2.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 3.0 0 3.5
Endosome 1.4 1.1 0 0 – 0 0 0 0 0 0 0 2.7 3.5 3.4
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.1 1.6 0 0 – 0 0 0 0 0 0 0 3.5 1.0 2.4
Nucleus -2.2 -6.2 -8.1 -4.7 – -5.6 -6.4 -8.9 -8.3 -0.3 -0.9 -3.4 -6.3 -6.1 -6.8
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 2.1 0 0 – 0 0 0 0 0 0 0 0 3.0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 3.1 4.2 0 0 – 0 0 0 0 0 -1.3 0 -1.6 0.1 0
Vacuole -0.6 1.0 1.3 0.7 – 1.2 1.3 2.2 1.8 -0.4 -1.2 -0.4 4.3 2.6 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0046 -0.2161 -0.5991 -0.7804 -1.1363 -0.4502 0.8687 0.5786 0.82 1.2327 0.3283 0.6213 0.8333 0.567 0.4933 0.3842 0.4234 0.7254
Actin 0.0701 0.0008 0.0008 0.0002 0.0012 0.0041 0.0275 0.0009 0.013 0.0123 0.0292 0.003 0.0293 0.0014 0.0004 0.0036 0.0214 0.0025
Bud 0.0013 0.0016 0.0002 0 0.0001 0.0003 0.0006 0.0002 0.0006 0.0024 0.0004 0.0002 0.0001 0.0078 0.0001 0.0002 0.0005 0.001
Bud Neck 0.0107 0.0011 0.0131 0.0003 0.0027 0.0081 0.0127 0.0007 0.0114 0.0065 0.003 0.0098 0.0031 0.0009 0.0012 0.0014 0.0012 0.0076
Bud Periphery 0.0021 0.0003 0.0002 0 0.0005 0.0006 0.0013 0.0002 0.001 0.0126 0.001 0.0005 0.0002 0.0013 0.0002 0.0002 0.0011 0.0003
Bud Site 0.0111 0.0043 0.0025 0 0.0003 0.0005 0.0091 0.0022 0.0075 0.0037 0.0018 0.0012 0.0005 0.0033 0.0006 0.0003 0.0014 0.0002
Cell Periphery 0.0006 0.0002 0.0001 0 0.0002 0.0001 0.0006 0.0001 0.0003 0.0006 0.0002 0.0001 0.0001 0.0001 0 0 0.0001 0
Cytoplasm 0.0402 0.0356 0.0277 0.0084 0.0509 0.0666 0.0309 0.0298 0.0162 0.0219 0.0354 0.0256 0.0269 0.0167 0.0211 0.0158 0.02 0.0181
Cytoplasmic Foci 0.0217 0.0063 0.0073 0.0072 0.0041 0.0048 0.0204 0.0013 0.0111 0.0228 0.0232 0.0024 0.0144 0.0016 0.002 0.0017 0.0227 0.0024
Eisosomes 0.0005 0.0001 0 0 0.0001 0 0.0006 0 0.0002 0.0002 0.0003 0.0001 0.0002 0.0001 0 0 0.0002 0
Endoplasmic Reticulum 0.0062 0.0035 0.0015 0.0011 0.0022 0.002 0.0069 0.0025 0.0025 0.0042 0.0024 0.0015 0.0059 0.0017 0.0016 0.0046 0.0045 0.0012
Endosome 0.0505 0.0069 0.0136 0.0033 0.0228 0.0087 0.0237 0.0027 0.0141 0.0491 0.0237 0.0052 0.0096 0.0026 0.0023 0.0087 0.0204 0.0053
Golgi 0.0193 0.0014 0.005 0.0012 0.0108 0.0082 0.0046 0.0003 0.0039 0.0173 0.0142 0.0017 0.0023 0.001 0.0002 0.0006 0.0056 0.0011
Lipid Particles 0.0222 0.0076 0.0021 0.0407 0.0273 0.0097 0.01 0.0006 0.0073 0.0394 0.0316 0.0046 0.0033 0.0011 0.0011 0.0004 0.0137 0.0055
Mitochondria 0.0147 0.0113 0.0111 0.0007 0.0171 0.0061 0.0055 0.0006 0.0045 0.0108 0.0074 0.0059 0.0008 0.0035 0.0005 0.0008 0.0063 0.0007
None 0.0633 0.0978 0.0591 0.043 0.0684 0.0356 0.0654 0.0469 0.066 0.0987 0.0539 0.0664 0.034 0.0163 0.0172 0.0152 0.018 0.0323
Nuclear Periphery 0.0422 0.0742 0.0331 0.0323 0.0201 0.0283 0.0432 0.0253 0.0316 0.0236 0.0172 0.0183 0.0305 0.0295 0.0468 0.0423 0.0368 0.0143
Nucleolus 0.0183 0.0235 0.0338 0.0059 0.0088 0.0234 0.0186 0.0285 0.0426 0.0116 0.021 0.0406 0.021 0.0356 0.0342 0.0432 0.0217 0.0472
Nucleus 0.4946 0.6298 0.6027 0.8181 0.6716 0.713 0.5722 0.733 0.6214 0.4576 0.6107 0.7103 0.6574 0.7415 0.6979 0.7583 0.6634 0.7472
Peroxisomes 0.0147 0.0069 0.0004 0.0177 0.0007 0.0017 0.0189 0.0002 0.0076 0.0249 0.0203 0.0016 0.0015 0.0003 0.0002 0.0001 0.0325 0.001
Punctate Nuclear 0.0855 0.0834 0.1832 0.0192 0.0817 0.0758 0.1194 0.1222 0.1348 0.1651 0.0999 0.0985 0.1565 0.132 0.1708 0.1006 0.1046 0.1107
Vacuole 0.0068 0.0023 0.0017 0.0003 0.0042 0.0016 0.0054 0.0015 0.0018 0.0107 0.002 0.002 0.0015 0.0013 0.001 0.001 0.0017 0.0011
Vacuole Periphery 0.0035 0.001 0.0007 0.0003 0.0042 0.0008 0.0024 0.0004 0.0008 0.0041 0.0012 0.0006 0.0009 0.0005 0.0005 0.0009 0.0021 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.0692 6.0421 6.3516 6.6037 7.3028 5.0664 7.7638 9.9646 6.8157 9.4028
Translational Efficiency 0.8731 0.4804 0.7016 0.7173 0.5825 1.1837 0.572 0.491 0.7821 0.6153

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages nucleus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Apc4

Apc4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apc4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available