Standard name | |
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Human Ortholog | |
Description | Pentafunctional arom protein; catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 8 | 9 | 4 | 17 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 0 | 2 | 4 | 1 | 10 | 10 | 24 | 19 | 20 | 16 | 21 | 0 | 2 | 3 | 1 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 316 | 128 | 153 | 159 | 194 | 166 | 217 | 288 | 212 | 227 | 124 | 171 | 95 | 202 | 218 | 129 | 90 | 121 | 305 | 131 | 146 | 84 | 141 | 164 |
Endoplasmic Reticulum | 5 | 4 | 0 | 1 | 7 | 3 | 18 | 34 | 21 | 26 | 24 | 19 | 2 | 1 | 1 | 5 | 6 | 9 | 6 | 0 | 3 | 4 | 6 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 7 | 12 | 11 | 26 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 1 | 2 |
Unique Cell Count | 321 | 133 | 153 | 159 | 201 | 170 | 239 | 335 | 248 | 272 | 168 | 227 | 95 | 203 | 219 | 130 | 93 | 122 | 320 | 140 | 153 | 94 | 157 | 185 |
Labelled Cell Count | 327 | 134 | 155 | 165 | 203 | 182 | 252 | 357 | 271 | 299 | 184 | 263 | 97 | 205 | 223 | 136 | 101 | 132 | 320 | 140 | 153 | 94 | 157 | 185 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 81.2 | 60.9 | 43.6 | 44.5 | 41.5 | 35.1 | 31.6 | 29.1 | 27.8 | 25.8 | 25.0 | 24.2 | 65.3 | 60.3 | 54.3 | 67.3 | 81.8 | 82.5 | 65.1 | 63.6 | 69.6 |
Std Deviation (1e-4) | 18.3 | 13.4 | 9.2 | 12.4 | 10.8 | 8.0 | 7.4 | 7.4 | 6.9 | 6.5 | 6.9 | 6.7 | 13.6 | 11.1 | 9.3 | 20.1 | 23.7 | 18.5 | 16.2 | 17.9 | 20.9 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.07 | -0.31 | -0.46 | -0.58 | -0.65 | -0.76 | -0.8 | -0.85 | 0.58 | 0.47 | 0.32 | 0.63 | 0.91 | 0.92 | 0.58 | 0.54 | 0.67 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 2.2 | 0 | 2.7 | 2.8 | 2.7 | 3.2 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | -2.3 | -1.9 | -3.9 | -4.9 | -4.9 | -5.3 | -6.8 | -6.7 | 0 | -0.9 | -0.8 | -1.1 | -2.2 | -1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3.5 | 4.1 | 3.7 | 3.9 | 4.9 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 57.0218 | 83.9 | 74.2645 | 78.2528 | 71.3698 | 74.5059 | 72.8912 | 97.9468 | 90.3695 | 85.0061 | 79.5683 | 91.6436 | 71.5368 | 97.9165 | 88.2833 | 82.7394 | 76.262 | 89.2349 |
Actin | 0.0219 | 0.0005 | 0.0012 | 0.0001 | 0.0002 | 0.0016 | 0.0063 | 0.0001 | 0.0002 | 0.003 | 0.0006 | 0.0004 | 0.0129 | 0.0007 | 0.0042 | 0.0152 | 0.0776 | 0.0025 |
Bud | 0.003 | 0.0006 | 0.0019 | 0.0008 | 0.0012 | 0.0054 | 0.0014 | 0.0002 | 0.0004 | 0.0006 | 0.0009 | 0.0012 | 0.0016 | 0.0002 | 0.0008 | 0.0042 | 0.0014 | 0.0015 |
Bud Neck | 0.0012 | 0.0007 | 0.0009 | 0.0011 | 0.0028 | 0.0061 | 0.0004 | 0.0003 | 0.0005 | 0.0009 | 0.0005 | 0.0008 | 0.0009 | 0.0002 | 0.0007 | 0.0016 | 0.0028 | 0.0009 |
Bud Periphery | 0.0031 | 0.0004 | 0.0016 | 0.0003 | 0.0007 | 0.0073 | 0.0011 | 0.0001 | 0.0005 | 0.0005 | 0.0009 | 0.0012 | 0.0012 | 0.0001 | 0.0005 | 0.0017 | 0.001 | 0.001 |
Bud Site | 0.009 | 0.0008 | 0.0017 | 0.0001 | 0.0002 | 0.0007 | 0.0013 | 0.0004 | 0.0003 | 0.0007 | 0.0003 | 0.0002 | 0.0011 | 0.0003 | 0.0038 | 0.0102 | 0.0147 | 0.0001 |
Cell Periphery | 0.0134 | 0.0215 | 0.0053 | 0.0017 | 0.0053 | 0.0131 | 0.0093 | 0.0072 | 0.0075 | 0.0064 | 0.006 | 0.0068 | 0.004 | 0.0009 | 0.0021 | 0.0014 | 0.001 | 0.0011 |
Cytoplasm | 0.5778 | 0.881 | 0.8045 | 0.7129 | 0.8087 | 0.7164 | 0.8224 | 0.9665 | 0.9065 | 0.8202 | 0.8802 | 0.9156 | 0.8121 | 0.9835 | 0.9187 | 0.8464 | 0.8136 | 0.8939 |
Cytoplasmic Foci | 0.0078 | 0.0006 | 0.0089 | 0.0007 | 0.0016 | 0.0025 | 0.0121 | 0.0005 | 0.0082 | 0.0026 | 0.0073 | 0.003 | 0.0114 | 0.0008 | 0.0018 | 0.0035 | 0.0114 | 0.0049 |
Eisosomes | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0.001 | 0.0001 | 0 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0556 | 0.035 | 0.0239 | 0.0018 | 0.0082 | 0.0314 | 0.0251 | 0.0043 | 0.0117 | 0.0068 | 0.0033 | 0.0043 | 0.0248 | 0.0028 | 0.0145 | 0.0013 | 0.0044 | 0.0332 |
Endosome | 0.0109 | 0.0012 | 0.0098 | 0.0032 | 0.0068 | 0.0093 | 0.0067 | 0.0007 | 0.0023 | 0.0043 | 0.0054 | 0.0066 | 0.0131 | 0.0009 | 0.0034 | 0.0057 | 0.0204 | 0.0143 |
Golgi | 0.0024 | 0.0002 | 0.0016 | 0.0001 | 0.0002 | 0.0009 | 0.0028 | 0.0001 | 0.0003 | 0.0008 | 0.0005 | 0.0006 | 0.0041 | 0.0004 | 0.0004 | 0.0008 | 0.0296 | 0.0022 |
Lipid Particles | 0.0041 | 0.002 | 0.0022 | 0.0001 | 0.0005 | 0.0011 | 0.0114 | 0.0006 | 0.0021 | 0.0051 | 0.0029 | 0.0003 | 0.0046 | 0.0001 | 0.0009 | 0.001 | 0.0022 | 0.0009 |
Mitochondria | 0.0103 | 0.0002 | 0.004 | 0.0001 | 0.0003 | 0.0026 | 0.0068 | 0 | 0.0005 | 0.0149 | 0.01 | 0.0037 | 0.0064 | 0.0001 | 0.004 | 0.0006 | 0.0034 | 0.0015 |
None | 0.0045 | 0.0002 | 0.0008 | 0.0001 | 0.0002 | 0.0004 | 0.0155 | 0.0001 | 0.0013 | 0.0044 | 0.0022 | 0.0006 | 0.003 | 0.0001 | 0.0003 | 0.0008 | 0.0003 | 0.0008 |
Nuclear Periphery | 0.0618 | 0.0051 | 0.0125 | 0.0079 | 0.0206 | 0.025 | 0.0105 | 0.0011 | 0.0027 | 0.0083 | 0.0232 | 0.002 | 0.0122 | 0.001 | 0.0036 | 0.0046 | 0.0011 | 0.0192 |
Nucleolus | 0.0084 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0006 | 0.0055 | 0 | 0.0004 | 0.0012 | 0.0007 | 0.0006 | 0.0016 | 0 | 0.0002 | 0.0005 | 0.0001 | 0.0003 |
Nucleus | 0.024 | 0.0027 | 0.005 | 0.0045 | 0.0057 | 0.0103 | 0.0077 | 0.0009 | 0.0021 | 0.0034 | 0.0125 | 0.0021 | 0.0046 | 0.0008 | 0.0018 | 0.0038 | 0.0012 | 0.0029 |
Peroxisomes | 0.0017 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0033 | 0 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.003 | 0 | 0.0003 | 0.001 | 0.0008 | 0.0001 |
Punctate Nuclear | 0.0035 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0012 | 0 | 0.0065 | 0.0004 | 0.0003 | 0 | 0.0013 | 0 | 0.0001 | 0.0009 | 0.0002 | 0.0023 |
Vacuole | 0.1449 | 0.0444 | 0.0921 | 0.2275 | 0.1126 | 0.1383 | 0.0422 | 0.0163 | 0.0426 | 0.1053 | 0.0301 | 0.0378 | 0.0611 | 0.0066 | 0.0316 | 0.0779 | 0.0087 | 0.0112 |
Vacuole Periphery | 0.0304 | 0.0028 | 0.0208 | 0.0371 | 0.0237 | 0.0268 | 0.0066 | 0.0007 | 0.0032 | 0.0089 | 0.0115 | 0.0123 | 0.0145 | 0.0005 | 0.0063 | 0.0165 | 0.0039 | 0.0051 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 128.5801 | 123.3229 | 122.8334 | 133.0275 | 142.551 | 130.2439 | 146.2407 | 146.2889 | 148.4481 | 160.3466 |
Translational Efficiency | 1.1483 | 1.1334 | 1.0223 | 0.997 | 0.9173 | 1.0071 | 0.9398 | 0.7542 | 0.778 | 0.7877 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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