Standard name | |
---|---|
Human Ortholog | |
Description | Subunit of SEACAT, a subcomplex of the SEA complex; Mtc1p, along with Rtc1p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; relative distribution to the vacuolar membrane decreases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 1 | 2 | 4 | 3 | 9 | 16 | 13 | – | 13 | 10 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 3 | – | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 17 | 20 | 25 | 62 | 174 | 196 | 377 | 447 | 295 | – | 230 | 290 | 39 | 74 | 111 | 80 | 47 | 62 | 0 | 2 | 4 | 5 | 7 | 7 |
Endoplasmic Reticulum | 4 | 4 | 7 | 6 | 0 | 4 | 0 | 8 | 0 | – | 1 | 1 | 0 | 7 | 7 | 13 | 4 | 6 | 0 | 0 | 2 | 1 | 1 | 2 |
Endosome | 64 | 7 | 1 | 1 | 35 | 0 | 1 | 4 | 1 | – | 0 | 1 | 10 | 27 | 17 | 29 | 12 | 23 | 86 | 17 | 22 | 15 | 38 | 76 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 49 | 30 | 79 | 101 | 136 | 204 | 290 | 408 | 232 | – | 213 | 337 | 10 | 17 | 54 | 17 | 10 | 12 | 11 | 6 | 10 | 1 | 6 | 4 |
Nucleus | 13 | 1 | 5 | 1 | 3 | 5 | 1 | 4 | 1 | – | 0 | 2 | 3 | 6 | 3 | 7 | 0 | 6 | 2 | 2 | 1 | 1 | 4 | 7 |
Nuclear Periphery | 12 | 9 | 0 | 2 | 0 | 0 | 1 | 3 | 0 | – | 0 | 0 | 1 | 3 | 2 | 0 | 1 | 2 | 14 | 3 | 3 | 1 | 7 | 9 |
Nucleolus | 26 | 4 | 1 | 3 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 2 | 2 | 0 | 1 | 1 | 1 | 3 | 1 | 0 | 0 | 5 | 1 |
Peroxisomes | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 15 | 3 | 0 | 1 | 3 | 1 | 1 | 3 | 2 | – | 3 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 6 | 1 | 0 | 1 | 6 | 4 |
Vac/Vac Membrane | 131 | 89 | 48 | 53 | 142 | 63 | 67 | 39 | 21 | – | 12 | 19 | 129 | 201 | 176 | 141 | 53 | 109 | 121 | 104 | 83 | 98 | 140 | 175 |
Unique Cell Count | 227 | 128 | 123 | 160 | 360 | 342 | 558 | 726 | 402 | 330 | 460 | 169 | 286 | 303 | 241 | 102 | 184 | 252 | 146 | 134 | 132 | 230 | 300 | |
Labelled Cell Count | 332 | 167 | 167 | 232 | 499 | 476 | 750 | 936 | 568 | 473 | 665 | 195 | 339 | 372 | 292 | 128 | 225 | 252 | 146 | 134 | 132 | 230 | 300 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 4.8 | 3.8 | 3.8 | 4.3 | 3.7 | 3.6 | 3.6 | 3.4 | – | 3.3 | 3.0 | 5.7 | 5.6 | 5.0 | 6.4 | 6.6 | 7.0 | 5.3 | 5.4 | 5.8 |
Std Deviation (1e-4) | 0.8 | 0.9 | 0.7 | 0.8 | 1.4 | 1.0 | 1.2 | 0.9 | 1.3 | – | 1.1 | 0.7 | 1.4 | 1.1 | 1.3 | 1.5 | 1.6 | 1.7 | 1.2 | 1.5 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.18 | -0.03 | -0.08 | -0.09 | -0.17 | – | -0.2 | -0.36 | 0.57 | 0.56 | 0.38 | 0.74 | 0.78 | 0.87 | 0.46 | 0.5 | 0.6 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.5 | 1.9 | 3.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.503 | 3.4965 | 3.48 | 2.9923 | 2.5802 | 3.1839 | 1.6879 | 2.2403 | 1.9198 | 2.2192 | 1.6093 | 1.8419 | -0.1796 | 0.3639 | -0.1637 | -0.3282 | -0.2494 | -0.129 |
Actin | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.001 | 0.0405 | 0.0001 | 0.0128 | 0.0425 | 0.0037 | 0.0017 | 0.0513 | 0.0259 | 0.0087 | 0.0001 | 0.0018 | 0.0011 |
Bud | 0.0003 | 0.0007 | 0.0008 | 0.0008 | 0.0003 | 0.0006 | 0.0008 | 0.0005 | 0.0009 | 0.0028 | 0.0002 | 0.0007 | 0.0005 | 0.0006 | 0.0004 | 0.0034 | 0.0004 | 0.0002 |
Bud Neck | 0.0004 | 0.0006 | 0.0053 | 0.0004 | 0.0004 | 0.0009 | 0.0013 | 0.0005 | 0.0062 | 0.0021 | 0.0003 | 0.0009 | 0.0019 | 0.0008 | 0.0003 | 0.0007 | 0.0003 | 0.0006 |
Bud Periphery | 0.0005 | 0.0018 | 0.0016 | 0.0018 | 0.0007 | 0.0013 | 0.0011 | 0.0005 | 0.0011 | 0.0032 | 0.0004 | 0.0009 | 0.0006 | 0.0007 | 0.0005 | 0.0085 | 0.0012 | 0.0002 |
Bud Site | 0.0009 | 0.0022 | 0.0016 | 0.0006 | 0.0006 | 0.0004 | 0.0038 | 0.0011 | 0.0023 | 0.0024 | 0.0004 | 0.0008 | 0.0025 | 0.0039 | 0.0014 | 0.0015 | 0.0005 | 0.0002 |
Cell Periphery | 0.0015 | 0.0007 | 0.0009 | 0.001 | 0.0015 | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0005 | 0.0005 | 0.0004 | 0.0005 | 0.0002 | 0.0001 |
Cytoplasm | 0.1131 | 0.1006 | 0.1216 | 0.03 | 0.0765 | 0.1278 | 0.0668 | 0.1077 | 0.0823 | 0.0515 | 0.044 | 0.0562 | 0.0552 | 0.0938 | 0.0859 | 0.0403 | 0.0576 | 0.08 |
Cytoplasmic Foci | 0.0363 | 0.0584 | 0.0508 | 0.0442 | 0.031 | 0.0444 | 0.0364 | 0.0236 | 0.0315 | 0.041 | 0.0358 | 0.024 | 0.0743 | 0.0393 | 0.06 | 0.0222 | 0.0594 | 0.0513 |
Eisosomes | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.012 | 0.0114 | 0.0123 | 0.009 | 0.016 | 0.0084 | 0.0064 | 0.0075 | 0.0089 | 0.003 | 0.0061 | 0.0036 | 0.007 | 0.0027 | 0.0101 | 0.0025 | 0.002 | 0.0038 |
Endosome | 0.3686 | 0.3834 | 0.41 | 0.4543 | 0.5013 | 0.3878 | 0.3257 | 0.2811 | 0.4357 | 0.3516 | 0.4375 | 0.3729 | 0.3107 | 0.3685 | 0.3831 | 0.471 | 0.4033 | 0.4666 |
Golgi | 0.0059 | 0.0063 | 0.0094 | 0.0124 | 0.008 | 0.0071 | 0.0175 | 0.0042 | 0.0093 | 0.0327 | 0.0177 | 0.0083 | 0.0095 | 0.0079 | 0.0096 | 0.0042 | 0.0144 | 0.0079 |
Lipid Particles | 0.017 | 0.0146 | 0.0088 | 0.0052 | 0.0041 | 0.0073 | 0.0209 | 0.0019 | 0.0055 | 0.0238 | 0.013 | 0.0029 | 0.0208 | 0.0159 | 0.0195 | 0.0095 | 0.0549 | 0.0209 |
Mitochondria | 0.0071 | 0.0092 | 0.0263 | 0.0184 | 0.0275 | 0.0149 | 0.019 | 0.0085 | 0.0119 | 0.0363 | 0.015 | 0.0075 | 0.0054 | 0.0117 | 0.0052 | 0.0076 | 0.0451 | 0.004 |
None | 0.0764 | 0.0266 | 0.0565 | 0.0308 | 0.015 | 0.0346 | 0.06 | 0.0209 | 0.0055 | 0.0012 | 0.0272 | 0.0075 | 0.0578 | 0.1685 | 0.149 | 0.0834 | 0.1263 | 0.0694 |
Nuclear Periphery | 0.015 | 0.0115 | 0.0063 | 0.0047 | 0.0066 | 0.0099 | 0.0154 | 0.0136 | 0.0131 | 0.0094 | 0.0189 | 0.0095 | 0.0127 | 0.0033 | 0.0149 | 0.0035 | 0.0024 | 0.0063 |
Nucleolus | 0.001 | 0.0015 | 0.0016 | 0.0008 | 0.0003 | 0.0007 | 0.0018 | 0.0015 | 0.0008 | 0.0008 | 0.0006 | 0.0005 | 0.0024 | 0.0017 | 0.0014 | 0.0008 | 0.0008 | 0.0007 |
Nucleus | 0.0032 | 0.004 | 0.0035 | 0.0027 | 0.0021 | 0.0039 | 0.0106 | 0.0054 | 0.0041 | 0.0036 | 0.0016 | 0.0044 | 0.0031 | 0.0033 | 0.0067 | 0.003 | 0.0022 | 0.0031 |
Peroxisomes | 0.0006 | 0.0018 | 0.0017 | 0.0067 | 0.0003 | 0.0009 | 0.0104 | 0.0004 | 0.0011 | 0.0121 | 0.0015 | 0.0009 | 0.0307 | 0.0023 | 0.0059 | 0.0013 | 0.0252 | 0.0018 |
Punctate Nuclear | 0.0009 | 0.0011 | 0.0007 | 0.0003 | 0.0003 | 0.0006 | 0.0022 | 0.0003 | 0.0005 | 0.0034 | 0.001 | 0.0003 | 0.0061 | 0.0007 | 0.003 | 0.0006 | 0.0011 | 0.0026 |
Vacuole | 0.2618 | 0.289 | 0.2142 | 0.2878 | 0.2222 | 0.2289 | 0.2892 | 0.395 | 0.2791 | 0.2933 | 0.2767 | 0.3408 | 0.2668 | 0.1883 | 0.1782 | 0.2767 | 0.1633 | 0.2115 |
Vacuole Periphery | 0.0775 | 0.0744 | 0.066 | 0.0881 | 0.0851 | 0.118 | 0.0694 | 0.1253 | 0.0869 | 0.0828 | 0.0978 | 0.1555 | 0.0798 | 0.0598 | 0.0557 | 0.0586 | 0.0376 | 0.0679 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 17.1257 | 23.4647 | 21.2339 | 25.2645 | 15.3561 | 17.2761 | 24.3038 | 25.6792 | 24.5151 | 24.5065 |
Translational Efficiency | 0.3475 | 0.2449 | 0.2967 | 0.2176 | 0.3116 | 0.341 | 0.2244 | 0.1878 | 0.2214 | 0.1869 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|