Standard name
Human Ortholog
Description Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.07 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0.1 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.55 0.56 0.64 0.53 0.34 0.44 0.49 0.56 0.47 0.56 0.45 0.55 0.69 0.65 0.67 0.53 0.47 0.55 0.35 0.32 0.39 0.71 0.74 0.67
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0
Endosome 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0.05 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0 0 0 0.11 0.12 0.09 0.09 0.35 0.23 0.32 0.31 0 0 0 0.09 0.05 0 0 0 0.06 0.06 0.06 0.07
Nucleus 0.06 0.09 0.07 0.12 0.16 0.2 0.17 0.13 0.1 0.11 0.13 0.12 0.27 0.28 0.23 0.11 0.12 0.07 0.09 0.1 0.1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.27 0.27 0.25 0.3 0.38 0.26 0.26 0.22 0.21 0.18 0.19 0.14 0 0 0 0.27 0.29 0.28 0.43 0.37 0.28 0.08 0 0
Vac/Vac Membrane 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.06 0.07 0.09 0 0.05 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 2 0 1 1 1 1 1 0 0 0 0 0 0 0 0 6 5 4 4 8
Bud 4 15 2 6 27 22 24 22 49 29 41 36 0 0 0 6 3 7 5 22 7 5 14 17
Bud Neck 0 8 14 4 36 23 26 32 30 17 23 27 0 0 1 2 2 6 1 4 2 1 4 1
Bud Site 0 4 0 2 5 9 3 0 15 9 15 14 0 0 0 10 6 1 – – – – – –
Cell Periphery 0 4 4 1 0 1 0 1 1 0 3 2 0 0 0 1 2 2 0 1 1 1 3 3
Cytoplasm 97 375 229 162 129 329 362 382 397 444 366 457 167 270 282 336 195 259 72 237 152 197 273 204
Endoplasmic Reticulum 0 1 1 0 0 1 0 1 1 0 0 0 1 0 0 33 25 30 0 0 2 0 0 5
Endosome 12 13 4 7 1 22 16 4 3 6 12 2 3 6 4 39 27 28 7 34 11 7 4 3
Golgi 0 0 0 0 0 1 1 0 0 0 0 0 1 1 0 1 0 2 0 2 1 3 5 11
Mitochondria 14 27 1 4 41 87 63 61 296 181 257 257 0 0 2 59 20 5 1 26 22 17 21 22
Nucleus 11 59 25 38 61 149 124 90 81 87 102 96 65 114 95 70 52 34 18 74 40 3 2 0
Nuclear Periphery 1 1 0 1 1 1 1 0 1 0 1 0 0 1 0 1 1 0 0 1 1 1 1 3
Nucleolus 10 5 0 4 5 9 8 7 9 8 7 4 0 1 0 11 11 12 0 2 2 0 0 1
Peroxisomes 1 3 1 3 0 5 3 0 6 2 5 1 0 0 2 19 4 7 0 2 0 1 1 1
SpindlePole 48 180 90 91 141 194 193 149 174 143 158 118 3 4 4 170 123 131 88 272 111 22 12 7
Vac/Vac Membrane 9 36 17 13 6 19 12 15 8 10 12 5 7 28 39 41 29 40 7 34 18 2 3 3
Unique Cell Count 176 665 358 308 375 751 741 682 838 788 814 833 243 414 418 639 417 468 207 738 391 278 368 307
Labelled Cell Count 207 731 389 336 455 872 837 765 1072 937 1003 1019 247 425 429 799 500 564 207 738 391 278 368 307


Cytoplasm, Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.8 5.1 4.9 4.5 4.4 4.3 4.6 3.5 3.8 3.6 3.6 5.3 5.1 4.9 5.3 6.6 6.8 6.2 6.1 6.8
Std Deviation (1e-4) 0.5 0.6 1.2 2.1 1.6 1.8 1.4 1.5 1.1 1.1 0.9 0.7 1.5 1.0 1.3 6.3 9.6 7.0 3.5 4.0 4.7
Intensity Change (Log2) – – – -0.07 -0.2 -0.23 -0.26 -0.16 -0.56 -0.41 -0.52 -0.51 0.04 -0.01 -0.06 0.05 0.37 0.41 0.27 0.26 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.1 1.3 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0951 3.828 3.5457 3.601 4.0334 3.6584 10.1939 5.3195 6.5146 5.7624 5.9647 5.3483 2.2209 2.84 3.2418 3.4815 3.1963 2.917
Actin 0.0076 0.0005 0.0363 0.0001 0.0025 0.0016 0.2367 0.0019 0.0449 0.0004 0.0539 0.0125 0.0187 0.0004 0.0067 0.0003 0.0002 0.0018
Bud 0.0019 0.0015 0.0002 0 0.0001 0.0001 0.0031 0.0068 0.0003 0 0.0003 0.0012 0.0033 0.0004 0.0003 0.0001 0.0003 0.0002
Bud Neck 0.0016 0.0023 0.0006 0.0003 0.0006 0.002 0.0049 0.0009 0.0106 0.0018 0.0018 0.0074 0.0045 0.0009 0.001 0.0063 0.0039 0.0014
Bud Periphery 0.0027 0.0024 0.0004 0 0.0001 0.0001 0.004 0.007 0.0003 0 0.0004 0.0009 0.0052 0.0004 0.0003 0.0002 0.0008 0.0003
Bud Site 0.0049 0.0038 0.001 0.0001 0.0005 0.0001 0.0079 0.0271 0.0036 0.0002 0.0012 0.0003 0.0039 0.0032 0.0009 0.0009 0.0008 0.0002
Cell Periphery 0.0006 0.0002 0.0001 0 0 0 0.0007 0.0005 0.0002 0 0.0001 0.0001 0.0007 0.0001 0.0002 0.0001 0.0006 0
Cytoplasm 0.1148 0.0708 0.0446 0.0028 0.0008 0.0728 0.0583 0.0704 0.1217 0.0035 0.001 0.1301 0.1081 0.0663 0.0507 0.0084 0.0178 0.0553
Cytoplasmic Foci 0.0834 0.0383 0.0093 0.0304 0.1456 0.0571 0.0831 0.076 0.0674 0.0604 0.103 0.0609 0.0928 0.0507 0.0138 0.0201 0.07 0.0389
Eisosomes 0.0004 0.0001 0.0006 0 0 0 0.0012 0.0001 0.0002 0 0.0002 0.0001 0.0004 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0068 0.0024 0.0012 0.0001 0.0002 0.0008 0.0205 0.0027 0.0021 0.0001 0.0004 0.003 0.0075 0.001 0.0017 0.0002 0.0054 0.0009
Endosome 0.0177 0.0071 0.0016 0.0003 0.0193 0.0025 0.0945 0.0015 0.015 0.0004 0.0068 0.0068 0.0207 0.0019 0.0016 0.001 0.0108 0.0044
Golgi 0.0023 0.0003 0.0012 0.0003 0.0044 0.0005 0.0194 0.0004 0.0055 0.0001 0.0076 0.0005 0.0019 0.0003 0.0004 0.0002 0.0025 0.0015
Lipid Particles 0.0161 0.002 0.0015 0.0039 0.073 0.0103 0.0539 0.002 0.0177 0.0002 0.1252 0.0038 0.0184 0.0017 0.0016 0.0016 0.0989 0.0057
Mitochondria 0.0052 0.0008 0.002 0.0001 0.0003 0.0003 0.0069 0.0022 0.0013 0.0001 0.001 0.0007 0.0073 0.0005 0.0004 0.0005 0.0007 0.0005
None 0.4146 0.3391 0.1291 0.002 0.0011 0.1618 0.09 0.3233 0.0971 0.0012 0.0006 0.1738 0.3366 0.2263 0.1179 0.0065 0.0527 0.1798
Nuclear Periphery 0.0159 0.0071 0.0073 0.0029 0.0042 0.0055 0.0635 0.0077 0.0093 0.0043 0.0021 0.0075 0.0158 0.0058 0.0065 0.014 0.0073 0.0026
Nucleolus 0.007 0.0078 0.0068 0.0041 0.0025 0.0042 0.0107 0.0055 0.0029 0.0096 0.0334 0.0053 0.0091 0.0075 0.0081 0.03 0.0182 0.0048
Nucleus 0.0943 0.2734 0.6465 0.6745 0.001 0.117 0.0889 0.1862 0.5411 0.6684 0.0768 0.1642 0.1176 0.3534 0.5838 0.6124 0.0565 0.0748
Peroxisomes 0.008 0.0006 0.0034 0.0014 0.0189 0.006 0.0184 0.0023 0.0033 0.0012 0.0101 0.0013 0.0055 0.0031 0.0002 0.0011 0.0072 0.0023
Punctate Nuclear 0.1817 0.233 0.1047 0.2765 0.7241 0.5565 0.1065 0.2711 0.054 0.248 0.5738 0.4154 0.2088 0.2728 0.2023 0.2955 0.6319 0.6236
Vacuole 0.0104 0.0057 0.0011 0.0001 0.0006 0.0007 0.0218 0.0031 0.0011 0.0001 0.0002 0.0036 0.0108 0.0029 0.0014 0.0004 0.0124 0.0006
Vacuole Periphery 0.0022 0.0006 0.0003 0 0.0002 0.0001 0.0051 0.001 0.0004 0 0.0001 0.0008 0.0022 0.0003 0.0002 0.0001 0.0012 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.5959 15.764 5.0132 8.6004 17.9643 5.0356 17.0775 18.0576 14.0386 14.2638
Translational Efficiency 1.5392 1.6728 2.708 1.6004 1.0516 4.0403 1.4354 1.2442 1.1492 1.3825

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages spindle pole (23%), nucleus (21%), mixed (8%)
Cell Cycle Regulation Yes
spindle pole - MA (0.00099)
nucleus - S/G2 (1e-05)
Subcompartmental Group N/A

Fin1

Fin1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fin1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available