Standard name | |
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Human Ortholog | |
Description | Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 2 |
Bud | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 5 | 3 | 8 | 2 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 1 | 0 | 1 | 2 | 0 | 1 | 2 | 1 | 0 | 0 | 1 | 2 | 2 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 266 | 246 | 163 | 156 | 229 | 155 | 155 | 294 | 185 | 257 | 86 | 100 | 293 | 422 | 395 | 362 | 220 | 141 | 258 | 230 | 159 | 185 | 224 | 211 |
Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 15 | 15 | 5 | 3 | 0 | 0 | 0 | 2 | 1 |
Endosome | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 3 | 3 | 3 | 3 | 0 | 1 | 3 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 15 | 4 | 6 | 17 | 22 | 29 | 77 | 106 | 85 | 143 | 58 | 63 | 0 | 0 | 2 | 12 | 4 | 2 | 4 | 3 | 5 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 2 | 0 | 1 | 1 | 1 | 3 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 4 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Vac/Vac Membrane | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 1 | 2 | 6 | 8 | 5 | 2 | 7 | 2 | 0 | 1 | 2 |
Unique Cell Count | 275 | 252 | 170 | 161 | 241 | 162 | 192 | 337 | 217 | 321 | 112 | 126 | 295 | 424 | 403 | 372 | 233 | 151 | 288 | 253 | 174 | 193 | 242 | 228 |
Labelled Cell Count | 283 | 253 | 177 | 177 | 259 | 187 | 235 | 415 | 277 | 416 | 149 | 169 | 297 | 425 | 406 | 405 | 257 | 164 | 288 | 253 | 174 | 193 | 242 | 228 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 5.0 | 3.9 | 3.8 | 4.1 | 3.4 | 2.5 | 3.0 | 3.0 | 2.7 | 2.9 | 2.8 | 5.2 | 4.9 | 4.9 | 5.4 | 6.8 | 6.9 | 4.9 | 5.4 | 5.6 |
Std Deviation (1e-4) | 0.7 | 0.8 | 0.7 | 1.3 | 1.4 | 1.6 | 0.4 | 0.9 | 0.9 | 0.9 | 1.0 | 1.2 | 1.0 | 1.0 | 1.3 | 1.6 | 1.9 | 1.9 | 1.0 | 1.8 | 1.0 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.05 | -0.2 | -0.67 | -0.38 | -0.41 | -0.54 | -0.46 | -0.49 | 0.39 | 0.32 | 0.3 | 0.46 | 0.79 | 0.8 | 0.32 | 0.45 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | -0.4 | -0.1 | -4.4 | -3.1 | -3.4 | -4.7 | -4.9 | -4.5 | 2.6 | 3.3 | 1.5 | 0.9 | -0.7 | -1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 3.0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.2 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0644 | 0.0029 | 0.0002 | 0.0035 | 0.0003 | 0.0007 | 0.0238 | 0.0009 | 0.0141 | 0.0043 | 0.0008 | 0.0094 | 0.1215 | 0.0005 | 0.0009 | 0.0001 | 0.0001 | 0.0033 |
Bud | 0.0022 | 0.0004 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0103 | 0.0001 | 0.0021 | 0.0041 | 0.0003 | 0.0004 | 0.0012 | 0 | 0.0001 | 0 | 0 | 0.0029 |
Bud Neck | 0.0028 | 0.0002 | 0.0001 | 0.0081 | 0.0003 | 0.0062 | 0.0012 | 0.0001 | 0.0007 | 0.0016 | 0.0002 | 0.0008 | 0.0021 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 |
Bud Periphery | 0.0064 | 0.0005 | 0.0001 | 0.0002 | 0.0005 | 0.0004 | 0.0037 | 0.0001 | 0.002 | 0.0092 | 0.0007 | 0.0004 | 0.001 | 0 | 0 | 0 | 0 | 0.0039 |
Bud Site | 0.0131 | 0.0021 | 0.0001 | 0.0379 | 0.0005 | 0.0001 | 0.0095 | 0.0007 | 0.0114 | 0.0035 | 0.0001 | 0.0005 | 0.0045 | 0.0003 | 0.0002 | 0 | 0 | 0.0001 |
Cell Periphery | 0.006 | 0.0004 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 |
Cytoplasm | 0.2141 | 0.4555 | 0.4219 | 0.3915 | 0.5969 | 0.5542 | 0.2045 | 0.4182 | 0.3184 | 0.6149 | 0.2439 | 0.3548 | 0.0018 | 0.5316 | 0.4005 | 0.3179 | 0.5418 | 0.4778 |
Cytoplasmic Foci | 0.0094 | 0.009 | 0.0034 | 0.0073 | 0.0025 | 0.0191 | 0.0244 | 0.0043 | 0.0334 | 0.0091 | 0.0237 | 0.0103 | 0.2365 | 0.0023 | 0.0018 | 0.0017 | 0.0016 | 0.0225 |
Eisosomes | 0.0024 | 0.0002 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0 | 0.0003 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0075 | 0.0068 | 0.0021 | 0.0034 | 0.002 | 0.0018 | 0.0026 | 0.0014 | 0.0061 | 0.0017 | 0.0012 | 0.0019 | 0.0003 | 0.0011 | 0.0013 | 0.0014 | 0.0066 | 0.0013 |
Endosome | 0.0408 | 0.0463 | 0.0012 | 0.0038 | 0.0034 | 0.0062 | 0.0187 | 0.0006 | 0.0197 | 0.0017 | 0.0206 | 0.0076 | 0.0592 | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.0007 |
Golgi | 0.0063 | 0.0078 | 0.0001 | 0.0004 | 0.0002 | 0.0011 | 0.0068 | 0.0002 | 0.011 | 0.0008 | 0.0195 | 0.005 | 0.1652 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 |
Lipid Particles | 0.0155 | 0.0014 | 0.0002 | 0.0003 | 0.0001 | 0.0024 | 0.0282 | 0.0001 | 0.0056 | 0.0001 | 0.0138 | 0.0039 | 0.0481 | 0 | 0.0001 | 0 | 0.0001 | 0.0003 |
Mitochondria | 0.022 | 0.0284 | 0.0004 | 0.0004 | 0.0047 | 0.0027 | 0.0093 | 0.0001 | 0.0117 | 0.004 | 0.0454 | 0.0012 | 0.0563 | 0 | 0.0001 | 0 | 0 | 0.0002 |
None | 0.3566 | 0.424 | 0.5666 | 0.5264 | 0.3501 | 0.3855 | 0.6151 | 0.5703 | 0.5415 | 0.3404 | 0.6197 | 0.5943 | 0.0007 | 0.4625 | 0.5926 | 0.6769 | 0.4478 | 0.4829 |
Nuclear Periphery | 0.0446 | 0.0017 | 0.0006 | 0.0013 | 0.0028 | 0.0009 | 0.002 | 0.0004 | 0.003 | 0.0005 | 0.0007 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0004 | 0.0004 |
Nucleolus | 0.0133 | 0.0002 | 0.0001 | 0.0012 | 0.001 | 0.0002 | 0.0031 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 |
Nucleus | 0.0847 | 0.0019 | 0.0011 | 0.0052 | 0.0304 | 0.0061 | 0.0033 | 0.0008 | 0.0048 | 0.0013 | 0.0016 | 0.0007 | 0.0001 | 0.0005 | 0.0006 | 0.0004 | 0.0003 | 0.0007 |
Peroxisomes | 0.0112 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0039 | 0.0226 | 0.0005 | 0.006 | 0.0014 | 0.0058 | 0.007 | 0.2983 | 0 | 0 | 0 | 0 | 0.0009 |
Punctate Nuclear | 0.0148 | 0.0007 | 0.0004 | 0.0046 | 0.0007 | 0.0069 | 0.0046 | 0.0005 | 0.0048 | 0.0005 | 0.0006 | 0.0006 | 0.001 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0009 |
Vacuole | 0.0501 | 0.0083 | 0.0007 | 0.0024 | 0.0023 | 0.0009 | 0.0038 | 0.0003 | 0.002 | 0.0005 | 0.0008 | 0.0004 | 0.0006 | 0.0001 | 0.0002 | 0.0003 | 0.0005 | 0.0005 |
Vacuole Periphery | 0.0117 | 0.0012 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.001 | 0 | 0.0008 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0 | 0 | 0 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.6868 | 19.0515 | 12.3129 | 12.6925 | 18.7183 | 13.0549 | 19.8843 | 21.1794 | 16.9663 | 19.9562 |
Translational Efficiency | 0.7923 | 0.8239 | 0.6109 | 0.6059 | 0.7789 | 1.0018 | 0.7774 | 0.4901 | 0.6825 | 0.809 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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