Standard name
Human Ortholog
Description Dihydrolipoyl transsuccinylase; component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.06
Bud 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.06 0.05 0.12 0.08 0.1 0.07 0.1 0.09 0 0 0.08 0.1 0.06 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.12 0.06 0.06 0 0.05 0 0 0 0 0 0 0 0.08 0.2 0.27 0.23 0.3 0.23 0.22 0.15 0.12 0.16 0.19 0.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0.12 0.05 0.07 0.1 0.08 0.09 0.08
Mitochondria 0.81 0.81 0.87 0.86 0.87 0.89 0.78 0.8 0.8 0.84 0.85 0.89 0.78 0.63 0.53 0.05 0.06 0.05 0.38 0.45 0.49 0.39 0.34 0.29
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0.05 0 0.07 0.1 0.12 0.11 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.1 0 0 0.08 0.09 0.16 0.13 0.1 0.09 0.16 0 0 0 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0.25 0.1 0.15 0.1 0.08 0.06 0 0 0 0 0 0 0.16 0.32 0.31 0.47 0.37 0.38 0.23 0.13 0.21 0.26 0.23 0.21
SpindlePole 0.1 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0.08 0.07 0.26 0.26 0.23 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0.07 0.05 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 2 6 9 1 5 5 17
Bud 10 2 4 2 0 3 3 1 2 3 2 2 2 7 6 10 3 3 1 1 0 0 0 6
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 5
Bud Site 2 4 0 0 0 0 0 1 0 0 0 1 2 0 0 3 1 1 – – – – – –
Cell Periphery 4 5 1 0 0 3 0 1 0 0 0 1 4 6 4 4 3 1 0 0 0 0 0 0
Cytoplasm 12 4 3 4 5 8 16 12 7 7 11 11 8 17 28 18 7 4 4 3 0 0 1 3
Endoplasmic Reticulum 0 3 0 0 0 1 0 0 0 0 0 0 1 2 3 2 2 1 0 0 0 1 1 2
Endosome 43 8 4 1 4 0 3 2 1 0 0 0 15 76 98 42 37 17 83 19 8 29 37 60
Golgi 1 0 1 0 1 0 0 1 0 1 1 1 1 3 3 9 14 9 19 9 7 14 18 25
Mitochondria 293 101 59 50 68 131 105 121 56 87 93 107 142 238 193 9 7 4 143 61 36 71 66 88
Nucleus 0 2 0 0 0 0 3 2 0 2 0 0 1 1 0 0 0 0 0 0 0 0 0 1
Nuclear Periphery 3 3 3 3 3 10 14 18 8 7 6 4 3 3 2 2 0 0 2 0 1 1 1 1
Nucleolus 6 5 7 0 3 12 12 24 9 10 10 19 3 1 2 7 6 2 0 1 0 1 1 2
Peroxisomes 91 13 10 6 6 9 4 6 0 3 0 1 29 123 112 87 46 28 86 17 15 47 45 64
SpindlePole 37 3 2 3 0 2 1 3 1 1 4 0 11 32 27 49 32 17 7 1 0 5 5 12
Vac/Vac Membrane 15 7 3 1 4 3 4 2 1 3 2 1 11 24 22 8 9 4 8 7 2 1 3 3
Unique Cell Count 361 124 68 58 78 148 135 151 70 104 109 120 182 379 363 186 125 74 373 135 75 184 197 302
Labelled Cell Count 517 160 97 70 94 182 165 194 85 125 129 148 235 533 500 250 167 93 373 135 75 184 197 302


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 6.0 6.7 6.1 8.2 7.9 8.0 9.1 10.8 10.6 10.9 12.7 5.8 5.7 5.6 6.5 7.8 8.2 6.0 6.1 6.9
Std Deviation (1e-4) 1.5 2.0 1.8 2.0 3.5 2.6 2.5 2.8 3.5 5.3 5.0 5.9 1.5 2.0 1.5 1.9 2.0 1.9 3.2 2.3 3.3
Intensity Change (Log2) – – – -0.12 0.3 0.24 0.26 0.44 0.7 0.68 0.72 0.93 -0.21 -0.23 -0.25 -0.04 0.23 0.3 -0.14 -0.12 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 1.7 0 0 0 1.4 1.2 0 0 0 1.3 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0.6 2.8 3.8 3.1 3.8 2.9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 2.4 0
Mitochondria -0.1 0.1 0.4 -1.5 -1.2 -1.1 -0.6 -0.3 0.5 -1.5 -3.9 -5.2 -13.1 -11.4 -9.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1.4 1.7 1.5 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 -0.5 -0.3 1.1 0.5 -0.1 -0.2 1.1 0 0 0 0 0 0
Peroxisomes -0.7 -1.4 -2.1 0 0 0 -2.9 0 0 0.2 2.9 2.7 4.7 3.2 3.1
SpindlePole 0 0 0 0 0 0 0 0 0 0 1.6 0 4.1 3.9 3.5
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2784 6.5935 6.0577 5.8921 6.9732 5.7673 6.9634 7.1303 7.212 6.429 6.508 6.7683 9.9811 9.0599 8.9858 9.1398 7.9816 9.5446
Actin 0.0352 0.0227 0.0185 0.0003 0.0027 0.0192 0.0412 0.0159 0.0128 0.0003 0.0231 0.0264 0.0486 0.012 0.0083 0.015 0.0519 0.0103
Bud 0.0004 0.0004 0.0001 0 0.0001 0.0006 0.0003 0.0005 0.0003 0 0.0008 0.0002 0.0006 0.0003 0.0002 0.0002 0.0004 0.0001
Bud Neck 0.0076 0.0004 0.0001 0 0 0.0002 0.0003 0.0002 0.0002 0 0.0004 0.0002 0.0085 0.0001 0.0001 0.0001 0.0004 0.0001
Bud Periphery 0.0007 0.0005 0.0002 0 0.0001 0.0013 0.0005 0.0007 0.0004 0 0.0011 0.0003 0.0011 0.0005 0.0003 0.0003 0.0009 0.0001
Bud Site 0.0032 0.0022 0.0003 0.0001 0.0001 0.0004 0.0011 0.0047 0.001 0 0.0023 0.0008 0.0126 0.001 0.0006 0.0003 0.0009 0.0001
Cell Periphery 0.0019 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0012 0.0002 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0132 0.0001 0 0 0 0 0.0002 0.0001 0.0012 0 0.0001 0.0001 0.0029 0.0001 0.0001 0.0002 0.0002 0.0001
Cytoplasmic Foci 0.0333 0.0122 0.0057 0.0022 0.0005 0.0041 0.0169 0.0165 0.0092 0.0006 0.024 0.0062 0.0142 0.013 0.0053 0.0037 0.007 0.0024
Eisosomes 0.0006 0.0003 0.0002 0.0001 0.0002 0.0003 0.0008 0.0002 0.0002 0.0001 0.0002 0.0003 0.0005 0.0003 0.0003 0.0003 0.0007 0.0004
Endoplasmic Reticulum 0.0017 0.0001 0 0 0 0.0001 0.0002 0.0001 0.0003 0 0.0001 0.0002 0.0019 0 0.0001 0.0004 0.0007 0.0002
Endosome 0.0378 0.019 0.0081 0.0006 0.002 0.0077 0.0222 0.0227 0.0086 0.0002 0.0308 0.0106 0.0257 0.0097 0.0071 0.0105 0.0139 0.006
Golgi 0.0392 0.0551 0.0437 0.0018 0.0052 0.0526 0.0625 0.0712 0.0426 0.0006 0.1183 0.0463 0.0337 0.0463 0.037 0.0356 0.0914 0.0329
Lipid Particles 0.0701 0.0379 0.0246 0.0121 0.037 0.047 0.0798 0.0182 0.0299 0.0095 0.072 0.0368 0.0881 0.0581 0.0373 0.0086 0.0159 0.0232
Mitochondria 0.6209 0.7632 0.8547 0.9499 0.9419 0.7816 0.6553 0.746 0.8347 0.8964 0.5346 0.817 0.6742 0.7704 0.8441 0.8929 0.7908 0.885
None 0.0174 0.0001 0 0 0 0.0001 0.0004 0 0.0006 0 0.0001 0.0002 0.0025 0 0.0001 0.0008 0.0006 0.0002
Nuclear Periphery 0.0041 0.0004 0.0002 0 0 0.0001 0.0005 0.0001 0.0006 0 0.0001 0.0002 0.0127 0 0.0001 0.0018 0.0018 0.001
Nucleolus 0.0125 0.0005 0.0002 0.0001 0.0002 0.0164 0.0003 0.0001 0.0001 0.0001 0.0004 0.0001 0.0012 0.0006 0.0001 0.0001 0.0001 0.0002
Nucleus 0.0028 0.0001 0.0001 0 0.0001 0.0005 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0018 0.0001 0.0001 0.0001 0.0002 0.0001
Peroxisomes 0.0775 0.0768 0.0325 0.0325 0.0073 0.0639 0.1142 0.0975 0.0508 0.0917 0.1882 0.0509 0.0495 0.0815 0.0559 0.0268 0.0194 0.031
Punctate Nuclear 0.0101 0.0001 0 0 0 0.0001 0.0003 0.0001 0.0036 0 0.0003 0.0008 0.0058 0.0001 0.0002 0.001 0.0017 0.0001
Vacuole 0.0039 0.0018 0.002 0.0001 0.0005 0.0004 0.0009 0.0023 0.0006 0.0001 0.001 0.0005 0.0044 0.0027 0.0005 0.0004 0.0003 0.0005
Vacuole Periphery 0.0059 0.0058 0.0086 0.0002 0.002 0.0034 0.0019 0.0028 0.002 0.0003 0.0019 0.0017 0.0083 0.0028 0.0022 0.001 0.0008 0.0062

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 66.1884 58.1055 54.1589 61.5297 63.2059 70.5091 58.2131 57.3028 68.6578 65.616
Translational Efficiency 2.0288 1.9668 2.1034 2.2961 2.2605 2.2154 2.03 2.2957 2.0872 2.0856

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Dihydrolipoyl transsuccinylase; component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated
Localization
Cell Percentages mitochondrion (84%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Kgd2

Kgd2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kgd2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available